As the investments from the state and society in universities increased rapidly, the scientific and technological resources gained huge promotion. However, the efficiency of some instruments remained low, and the repeated purchases of some instruments occurred frequently. Here the authors discussed about building a practical, reasonable and efficient system for lab management.

Objective:To investigate whether superantigen SEB,with the assistance of APCs,could induce apoptosis of thymocytes in vitro,and to study the mechanism involved.Methods:SEB was given to thymocytes which were cultured together with APCs.Apoptosis was detected by DNA electrophoresis and DNA fragmentation assay.Expressions of Fas and FasL were detected by flow cytometry.Results:The thymocytes treated with SEB had the characters of apoptosis,and the apoptotic rate and expressions of Fas and FasL was significantly increased compared with control.Conclusion:SEB,together with APCs,can induce apoptosis of thymocytes in vitro,high expressions of Fas and FasL on thymocyte may participate in the mediation of apoptosis.This may provide a method to study the negative selection of thymocytes.

Objective:To explore and improve the teaching model and method of basic courses of computer application in Military Medical University so as to enhance the teaching quality and effect of the courses.Methods: Through analysis and discussion for the facing problem in basic teaching course of computer application of military medical university, and adopting series of teaching methods included that confirmed teaching aim, innovated teaching idea, explored group instruction and emphasized curriculum development, and combining teaching experiences, some opinions and suggestions were proposed to strengthen and improve the teaching quality of basis course of computer application.Results:After improved the teaching methods for basic courses of computer application, the project assessment and actual measurement of students' ability showed that the students' knowledge level and ability were greatly improved than formerly students, and the teaching level of the basic course of computer application also were enhanced.Conclusion: Under the new situation, basic course teaching of computer application in military medical university is facing new challenges, and the corresponding measurements should be adopted to enhance the teaching quality so as to satisfy the requirement of compound talent cultivation for military medicine under the informatization age.

Objective To compare the curative efficacy and prognosis between laparoscopic and open surgery in treatment of adults abdominal wall hernia. Methods 129 patients of adult abdominal wall hernia from January 2011 to April 2014 were selected as research objects. Retrospectively analyzed their clinical data and then divided them into control group (n = 62) and observation group (n = 67) according to operation method. The control group was given open surgery of herniorrhaphy, while the observation group was given laparoscopic surgery of herniorrhaphy. Then, operation features, postoperative complications incidence, inflammatory factors levels and recurrence rate were record and compared. Results All patients underwent successful operation with herniorrhaphy. In comparison with the control group, the observation group had statistically less bleeding volume during surgery [(21.60 ± 5.80) vs (61.60 ± 10.30) ml], shorter pain time, evacuation active time and hospitalization time [(30.60 ± 6.30) vs (45.20 ± 7.40) h, (14.70 ± 2.50) vs (20.80 ± 4.60) h, (5.30 ± 0.90) vs (8.70 ± 1.30) d, all P < 0.05], while the operation time between the two groups were statistically same (P > 0.05). The total incidence of postoperative complications in the observation was statistically lower than that in control group (14.9 % vs 33.9 %, P < 0.05). As to inflammatory factors, 24 hours and 48 hours after the surgery, levels of IL-6 and CRP in the observation group was respectively statistically lower in comparison with control group (all P < 0.05). After the two-year follow-up, the recurrence rate in the observation group was statistically the same with that in control group (1.5 % vs 6.5 %, P > 0.05). Conclusion Open and laparoscopic surgery of herniorrhaphy are both effective operation methods for adult patients with abdominal wall hernia. However, laparoscopic surgery of herniorrhaphy has less surgical trauma, and it showed more advantages in reducing incidence of postoperative complications, alleviating inflammatory damage caused by operation.

Objective To optimize the application of the nested PCR method for the detection of malaria according to the working practice,so as to improve the efficiency of malaria detection. Methods Premixing solution of PCR,internal primers for further amplification and new designed primers that aimed at two Plasmodium ovale subspecies were employed to optimize the reaction system,reaction condition and specific primers of P. ovale on basis of routine nested PCR. Then the specificity and the sensitivity of the optimized method were analyzed. The positive blood samples and examination samples of malaria were detected by the routine nested PCR and the optimized method simultaneously,and the detection results were compared and analyzed. Re-sults The optimized method showed good specificity,and its sensitivity could reach the pg to fg level. The two methods were used to detect the same positive malarial blood samples simultaneously,the results indicated that the PCR products of the two methods had no significant difference,but the non-specific amplification reduced obviously and the detection rates of P. ovale subspecies improved,as well as the total specificity also increased through the use of the optimized method. The actual detection results of 111 cases of malarial blood samples showed that the sensitivity and specificity of the routine nested PCR were 94.57％and 86.96％,respectively,and those of the optimized method were both 93.48％,and there was no statistically significant dif-ference between the two methods in the sensitivity(P>0.05),but there was a statistically significant difference between the two methods in the specificity(P<0.05). Conclusion The optimized PCR can improve the specificity without reducing the sensi-tivity on the basis of the routine nested PCR,it also can save the cost and increase the efficiency of malaria detection as less ex-periment links.

Objective:To study the relation of angiogenesis with cell proliferative postenti al in human meningiomas.Methods:Expression of factor Ⅷ-related antigen (FⅧRAg) and proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry in 38 specimens of human meningiomas,including 14 benign,12 atypical and 12 anaplastic (malignant) menningiomas and the correlation of microvessel density (MVD) with PCNA labellin g index (PCNA LI) was analyzed.Results:There was no significant difference in MVD between benign and atypical m eningiomas (P>0.05).MVD was higher in analplastic meningiomas than in both benign and atypical tumors (P<0.01).PCNA LI was markedly higher in anaplast ic meningiomas than in benign and atypical tumors (P<0.01). A significant c orrelation between MVD and PCNA LI was not detected in benign tumors;whereas,MVD positively correlated with PCNA LI in both atypical (r=0.518,P<0.01) and a naplastic (r=0.358,P<0.01) meningiomas. Conclusion:Angiogenesis is linked to cell proliferative potential in both atypic al and anaplastic meningiomas.

Objective To analyse the effects of Word Bank Loan Schistosomiasis Control Project in Hunan Province from 1992 to 2000. Methods The administrative villages in Hunan endemic areas were divided into heavy, moderate and low zones respectively, based on human infection rates. Control strategies including a major approach of chemotherapy to inhabitants and bovines with praziquantel, complemented with health education as well as snail control by environment modification, were adopted in each endemic zone. Schistosomiasis prevalence surveillance was carried out in 50 moving pilots selected from endemic villages in each zone every year. Results At the beginning of the programm, there were total of 35 endemic counties (cities, districts, farms) and 471 endemic towns in Hunan Province. Up to 2000, 8 endemic counties (farms) reach the transmission interruption criteria, and 25 counties and 300 towns reach the transmission control criteria. Heavy endemic villages decreased from 762 to 271, while moderate endemic villages from 1728 to 1053, and low endemic villages increased from 1447 to 2716. Human infection rates dropped from 11.59% to 5.64%. Conclusion The schistosomiasis epidemic situation decreased obviously after implementing the project in Hunan Province.

Intrahepatic bile duct stones located at the upper part of the hepatic duct.The percentage of intrahepatic biliary cholesterol calculus is increasing in recent years,and the incidence of this type of bile duct stones is free from infection or obstruction.The formation of intrahepatic bile duct stones might not only related to the micro-environment changes in the biliary tract,but also related to the changes of metabolic function of hepatocytes or cholangiocytes.In this article,the mechanism of biliary hydrodynamics on the formation of intrahepatic bile duct stones was reviewed.

Objective:To Observe the effect of recombinant tissue-type plasminogen activator (rt-PA) in treatment of acute myocardial infarct.Methods:Two groups of patients were observed.The subjects in the control group (35 patients) were treated with routine intervention,while those in thrombolysis group (15 patients) were treated with rt-PA in addition to the routine intervention.Results:There is a better outcome in the thrombolysis group as compared with that in the control group,in terms of length of angina relief,degree and length of EKG changes as well as the time for serum enzyme changes.The mortality rate in the thrombolysis group was significantly lower than that in the control group (P＜0.01).Conclusion:The rt-PA for thrombolysis in acute myocardial infarct patients can reduce the infarct size and improve the cardiac function rapidly as well as increase the survival rate.

Objective To investigate the effects of exercise on memory impairment in demented mice and possible molecular mechanisms.Methods Thirty Kunming nice were divided into a normal control group,a dementia group and a dementia plus exercise training group (exercise training group),10 mice/group. Dementia models were established by intraperitoneal injection of scopolamine.Exercise training was begun 30 min after the injection and lasted for a 6-week period. A water maze test was performed after the exercise training. Brain-derived neurotrophic factor (BDNF) expression and protein kinase (Akt) phosphorylation in the hippocampus were detected by Western blot analysis.Nitric oxide (NO) content and superoxide dismutase (SOD) activity in the hippocampus were also measured.ResultsDementia models were successfully induced as indicated by prolonged escape latency in the water maze test.After exercise training the escape latency had shortened significantly and hippocanpal BDNF expression and Akt phosphorylation had increased.NO content increased and SOD activity decreased after the injections,but these effects were partially reversed after exercise training.Conclusions Exercise can improve spatial memory significantly after scopolanine-induced dementia in mice.The increased hippocampal BDNF expression and Akt phosphorylation,increased SOD activity and decreased NO content may partially explain the behavior improvement induced by exercise.