Objective To discuss the therapeutic effects and safety of external ultrasound liposuction to adipose tissue of lower extremities and to summarize the operative techniques. Methods According to the requests of 81 patients, external ultrasound liposuction were performed for them on thighs (n=60), and legs (n=21). It was that the volumes of anesthetic drugs and pure fat as well as the variations of body contour between preoperation and one to three months after operation in every area were recorded. Results Fat aspiration volume in thighs was 3673ml per person with the highest 6100ml. The scale of the circumference of thighs decrease (4.65?1.10)cm with the highest 12.5 cm. In legs, fat volume was 650ml per person with the highest 1200ml, scale decrease (3?1.02) cm with the highest 9cm. Patients had less blood loss, less pain and discomfort with even skin appearance and without severe complications. In the course of operation, knowing well the anatomical character of lower extremities, injecting the anesthetic drugs completely, using appropriate ultrasound time, and sucking in uniform layer were all crucial factors to guarantee the success of the operation. Conclusion External ultrasound liposuction is a safe, effective body contour remodeling operation in lower extremities. Skillful operator is a very important element to successful operation.

Objective:To observe the acoustic characteristics of glottal stops produced by patients with cleft palate.Methods:Twenty boys with cleft palate who were judged to be glottal-stop-producers and 20 normal peers were included. In their own way, all the children were asked to produce following sounds: /iu/ /pai/ /t′u/, /s/ /k′/ /p′u/ /t′u/, /tA/ /ku/ and /i/ /k′u/ /ti/ /suan/. Then the speech spectroanalysis and the comparison were performed by one investigator.Results:The acoustic characteristics of glottal stops changed a lot when they substitute or accompany with different oral stops, which included the emergence of spikes, changes of voice onset time, the formant transitions and spetroanalysis of spikes. Conclusion:The acoustic changes of glottal stops are complicated. Every single aspects of acoustic analysis should be considered in the identification of the acoustic characteristics.

The problems of some hospital in medical equipment maintenance management were introduced from the aspects of maintenance time, maintenance record, maintenance service and self maintenance. Some measures were proposed including simplifying maintenance process through PDCA, informatizing maintenance record, cooperating with third-party maintainer and establishing self maintenance team, so that precision and informatized medical equipment management can be realized to maximize the benefit of medical equipment management.

Objective To explore the value of autoantibodies and biochemical markers in the diagnosis of autoimmune hepatitis (AIH),and to provide the basis for clinical diagnosis.Methods AIH patients as AIH group(n=35),viral hepatitis as viral hepatitis group (n=30,including 9 cases of hepatitis A,15 cases of hepatitis B and 6 cases of hepatitis C),the other 33 healthy volunteers were selected as healthy control group,indirect immunofluorescence assay was used to detect ANA,ASMA,AMA.Western blot was used to detect SLA/LP,LC-1,LKM,AMA-M2 and biochemical examination means ALT,AST,ALP,gamma-GT,T-BIL,D-BIL,TBA.Results The test results of autoantibodies were tested by chi square test.The positive rate of ANA,ASMA,AMA and AMA-M2 in AIH group was significantly higher than that in viral hepatitis group and healthy control group (P<0.05).The results of biochemical test by rank sum test,ALP,gamma-GT in the AIH group was significantly higher than the other two groups (P<0.05).The results of D-BIL detection in viral hepatitis group were significantly higher than those in the other two groups (P<0.05).Conclusion The combination of autoantibodies and laboratory biochemistry could help clinical diagnosis of AIH.

ObjectiveTo investigate the efficacy of acupuncture point injection for fine motor functions in children with cerebral palsy.Methods57 children with cerebral palsy were treated by acupuncture point injection combined with occupational therapy. The efficacy was evaluated by the Peabody Developmental Motor Scale-Fine Motor (PDMS-FM).ResultsThe fine motor quotient (FMQ) of 34 children (59.7%) elevated. The raw scores of grasping subtest (Gr) elevated in 45 children (78.9%) and the raw scores of visual-motor integration (VI) elevated in 53 children (93.0%).ConclusionThe efficacy of acupuncture point injection combined with occupational therapy is significant in children with cerebral palsy.

10.3969/j.issn.2095-4344.2013.24.026

Objective To analyze the endoscopic and pathologic characters of acute graft versus host disease(aGVHD) after aUogeneie bone marrow transplantation.Methods The endoscopic and pathologic findings of 4 patients with acute aGVHD were retrospectively analyzed.Results The clinical manifestations of 4 patients were abdominal pain and diarrhea occurred 21～57 days after bone marrow transplantation.The eolonoseopy detected mucosa edema,erosion and multiple ulcers.The pathological findings included epithelial necrosis accompanied with infiltration of lympbocytes and plasmacytes,no cytomegalovirus was found in biopsies,and aGVHD was diagnosed.Three patients recovered after the treatment with corticosteroids,while the other one died.Conclusion The intestine is involved in aGVHD after allogeneie bone marrow transplantation,and the diagnosis depends on eolonoscopy and biopsy.

BACKGROUND: Injectable artificial bone composite has been reported to consider as a moulding filler of vertebrae at the histology level; however, the therapeutic effect needs to be further studied at the level of axial pressure capacity. OBJECTIVE: To explore the axial pressure capacity of dogs with thoracolumbar fractures treated by injectable artificial bone composite. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at the Animal Laboratory of Shenzhen Longgang Central Hospital from September 2008 to January 2009. MATERIALS: Type I and type II vertebral pedicle screw systems were provided by Tianjin Zhengtian Medical Device Company Limited; injectable artificial bone composite, which was made of coral-hydroxylapatite compound, recombinant human bone morphogenetic protein-2 (Academy of Military Medical Sciences), and 2% chitosan solution (the Second Military Medical University of Chinese PLA and Shanghai Qisheng Biomaterials Institute), was manufactured according to the methods provided by Yin et al. METHODS: A total of 20 1-year-old healthy dogs were randomly divided into treatment and control groups with 10 dogs in each group. The model of those dogs with thoracolumbar fractures was made by imitating falling accidents; thereafter, the dogs in the treatment group were treated with vertebral pedicle screw system internal fixation and vertebroplasty by filling the injured vertebrae with injectable artificial bones. The control group was treated with vertebral pedicle screw system internal fixation alone. MAIN OUTCOME MEASURES: The maximum pressure intensity of injured vertebra, upper and lower vertebra at vertebral body center was detected using micro-computer pressure testing system after three months. RESULTS: The maximum pressure intensity at vertebral body center was not significant differences in the treatment group (100% cases) between injured vertebral body and its neighboring vertebral body, and axial loading was recovered. While that of 60% cases in the control group was significantly different (P

Objective To identify the point mutation of Cu/Zn superoxide dismutase(SOD1) gene in an amyotrophic lateral sclerosis(ALS) family and observe the value of denaturing high performance liquid chromatography(DHPLC). Methods DHPLC and DNA sequencing were used to examine SOD1 gene of the ALS family which had not been found mutation by PCR-SSCP. Results DHPLC tests proved double peaks in one member(Ⅲ_1), Which indicated the possibility of mutation in SOD1 exon 4. DNA sequencing revealed that there was a heterozygote,with mutation of GAA to GGA in exon 4, and with a substitution of glutacid by glycine. Conclusion As compared with PCR-SSCP, DHPLC technique has proved to be a rapid and reliable method for screening mutation site in large samples.

Objective:To investigate YKL-40-mediated inflammation in human bronchial epithelial cells and analyzed the soluble factors secreted by bronchial epithelial cells exposed to YKL-40 that were responsible for increasing proliferation and migration of primary normal human bronchial smooth muscle cells (BSMCs).Methods:YKL-40-induced inflammation was assayed in two human bronchial epithelial cells (BEAS-2B cell line and primary human bronchial epithelial cells ,namely HBECs).In addition,we treated BEAS-2B cells and HBECs with YKL-40,and added the conditioned culture media ( YKL-40-BEAS-2B-CM) and ( YKL-40-HBECs-CM) to BSMCs.The proliferation and migration of BSMCs were determined by premixed WST-1 cell proliferation reagent and QCM chemotaxis migration assay ,respectively.Results: Bronchial epithelial cells treated with YKL-40 resulted in a significant increase of IL-8 production,but have no effect about RANTES ,Eotaxin and TNF-α.YKL-40-BEAS-2B-CM and YKL-40-HBECs-CM induced IL-8 was found to further stimulate proliferation and migration of BSMCs ,and the effects were inhibited after neutralizing IL-8.Conclusion:Through investigating the interaction of airway epithelium and smooth muscle ,our findings implicate that YKL-40 may be involved in the inflammation of asthma by induction of IL-8 from epithelium,subsequently contributing to BSMCs proliferation and migration.Moreover, inhibition of IL-8 signaling is a potential therapeutic target for YKL-40-induced inflammation and remodeling of asthma.