Animals ; Bone Regeneration ; Bone Resorption ; Cells, Cultured ; Disease Models, Animal ; Humans ; Jaw ; metabolism ; Osteoblasts ; Osteoclasts

Objective:To investigate the effect of genistein,a soybean-derived isoflavone,on thestimulating effect on bone resorption of IL-1?.Methods:The osteoclasts(OCs)were isolated with themethods of Yu Shifeng.The rat calvaria were cultured as an organ.The cells in the experiment weregrown in four respectively:Control(without genistein or IL-1?),10~(-6) mol/L genistein,10 ?g/L IL-1?and 10~(-6) mol/L genistein+10 ?g/L IL-1?.The area of bone resorption,the concentration of Ca~(2+) in thesupernatant liquid of OCs cultures and mice calvaria were tested.The contents of acid phosphatase(ACP)were also examined by biochemistry method.The index of bone resorption was counted as the ra-tio of the experiment average and control ones,which indicated the increase in bone resorption when itwas above 1.0.Results:The area of bone resorption of 10~(-6) mol/L genistein+10 ?g/L IL-1? increasedcompared with that of 10~(-6) mol/L genistein,while the concentration of Ca~(2+) in the supernatant liquid ofOCs cultures decreased significantly.The index of bone resorption of 10~(-6) mol/L genistein+10 ?g/L IL-1? lied between 10~(-6) mol/L genistein and 10 ?g/L IL-1?.In the organ culture,there was no differencein the content of ACP among all the groups,The index of bone resorption of 10~(-6) mol/L genistein+10?g/L IL-1? was below that of 10?g/L IL-1?,but both were above 1.0.The index of bone resorptionwas below 1.0 in the group of 10~(-6) mol/L genistein.Conclusion:Genistein can suppress obviously thebone resorption simulated by single IL-1?.

Biomarkers, Tumor ; analysis ; Humans ; Male ; Prostate-Specific Antigen ; analysis ; Prostatic Neoplasms ; metabolism

Adult ; Female ; Hair Follicle ; pathology ; Humans ; Pilomatrixoma ; Skin Neoplasms

p21-Activated kinases including p21-activated kinase 2 contributed to the regulation of actin cytoskeleton and cell dynamics. In order to investigate the function of PAK2 on the maturation of Xenopus oocyte, PAK2-NT(PAK2-N-terminal,PAK2-NT) and PAK2-NTm (PAK2-N-terminal mutation) mRNA were microinjected into Xenopus oocyte respectively. Under fluorescent microscopy germinal vesicle breakdown was observed during cytokinesis. To further observe the relationship of oocyte cytokinesis, polar body formation and Cdc42 activity, confocal microscopy with time-lapse was employed . As a result, occurrences of germinal vesicle breakdown in oocytes were similar to those oocytes injected with PAK2-NT mRNA or injected with PAK2-NTm mRNA,but no cytokinesis and polar body formation were observed in oocytes injected with PAK2-NT mRNA or PAK2-NTm mRNA. These results indicated that PAK2 involved in Xenopus oocytes cytokinesis and polar body formation independent of Cdc42 activity.

This research is to investigate the effect of NIP regime in the treatment of non small cell lung cancer (NSCLC) and its side effects.Thirty two patients with NSCLC (stageⅢ 28 cases, include Ⅲa 6 cases and Ⅲb 22 cases, stageⅣ 4 cases) received chemotherapy regime NIP.Vinorelbine(NVB)25 mg/m 2 V.D,first day and eighth day ;Ifosfomide(IFO) 1.6 g mg/m 2 V.D, from first day to third day; Cisplatin(DDP)25 mg/m 2 V.D,from first day to third day.Four weeks was a cycle, every patients was given 2 cycles. RR was 53.13%(CR 1/32,PR 16/32,SD 4/32,PD 9/32). Main side effect was mylosuppression.NIP regime is effective and safe for patients with advanced NSCLC.

Objective To explore the clinical features of drug abuse mother’ s infants and evaluate the effect of corresponding therapeutics.Methods The clinical data of 54 cases of drug abuse mother’s infants were analyzed. Result During 12 years from 1990 to 2002,54 cases of drug abuse mother’ s infants were treated in our department. In 54 cases,premature was found in 28 cases,LBW in 32 cases,neonatal asphyxia 14 cases, NRDS 12 cases,intracranial hemorrhage 8 cases,congenital anomaly 2 cases. Infants born to addicted mothers have many health problems in the neonatal period Infants born to mothers drug abusing more than 2 years were significantly less mature and lighter than those born to mothers drug abusing less than 2 years( P

Based on the teaching practice of BME in higher Medical University for years,this article expounded the features,the teaching intension,the moving mechanism,etc.It is expected taht this article will improve the development greatly of BME in higher Medical University.

Cone-Beam Computed Tomography ; methods ; Four-Dimensional Computed Tomography ; methods ; Humans ; Image Processing, Computer-Assisted ; methods ; Magnetic Resonance Imaging ; methods ; Multimodal Imaging ; methods ; Neoplasms ; diagnosis ; diagnostic imaging ; radiotherapy ; Positron-Emission Tomography ; Radiotherapy, Computer-Assisted ; methods ; Tomography, X-Ray Computed

Child ; Child, Preschool ; Humans ; Sleep Apnea, Obstructive