Objective:To detect and analyze the membrane estrogen receptor(mER)in primary cultured interstitial cells of Cajal(ICC).Methods:Interstitial Cells of Cajal(ICC)'s surface binding sites for 17-?estradiol(E2)were detected by cell-impermeant ligand using confocal microscopy.Radioligand binding assay and Scatchard software were used to analyzed the characteristics of mER.Results:Immunofluorescence shows the staining pattern of nonfixed,nonpermeabilized ICC incubated with E2BSAFITC.The radioligand binding assay were analyzed by Scatchard software,The Bmax of mER was 45.75 fmol/mg protein and the kD was 0.717 3 nmol/L.Conclusion:A form of the estrogen receptor is present within the cell membrane of ICC and maybe capable of mediating rapid effect of estrogen.

Objective To investigate the influence of trimetazidine combined with atorvastatin on heart func-tion and the BNP level in patients with cornary heart failure.Methods The clinical data of 400 patients with cornary heart disease were retrospectively analyzed.194 patients in the control group were treated with conventional treatment, 206 patients in the observation group were treated with trimetazidine combined with atorvastatin on the basis of the control group.The treatment effect,cardiac function and BNP level were observed and compared.Results The effec-tive rate of the observation group was 91.26%,which was significantly higer than 76.29% of the control group (χ2 =16.667,P <0.05).After treatment,the cardiac index,stroke volume,cardiac output,left ventricular ejection fraction and BNP level of the two groups were significantly higher than before treatment,and those in the observation group were significantly higher than the control group(t =2.505,2.851,3.308,3.452,all P <0.05).Conclusion Trime-tazidine combined with atorvastatin in treatment of cornary heart failure has good curative effect,it can reduce the serum level of BNP,improve cardiac function significantly,which is of great value for clinical use.

Objective To explore the distribution and characterization of class 1 integrons in E.coli from healthy feces,and to elucidate the status of gene-cassettes.Methods Routine method was used to isolate E.coli,antibiotics susceptibility was tested by the disk diffusion method;class 1 integron was detected by PCR assay;PCR products were sequenced and analyzed.Results Of 97 samples,76 isolates were identified,and 25 isolates were multiple-drug resistant.The antibiogram was sulfamethoxazole-trimethoprim,ampicillin,streptomycin,tetracycline,erythromycin.14 of 25 isolates carried class 1 integrons,and the size of integrons differed from 1 800 bp(10 strains) to 750 bp(4 strains).The sequenced PCR product demonstrated that the 1 800 bp integron laboured aadA1-dfrA14-orf gene cassette conferred the resistance to sulfamethoxazole-timethoprim,streptomycin and aminoglycoside;the 750 bp integron laboured dfrA14 gene cassette conferred the resistance to sulfamethoxazole-trimethoprim.Conclusion The different kinds of class 1 integrons exist in E.coli from the healthy students,and determine the multiple-resistant antibiotics.

Owing to the advanced age,limb hemiplegia,dehydration,and vessel wall injury,stroke may be easy to cause venous thromboembolism (VTE).VTE mainly include deep vein thrombosis (DVT) and pulmonary embolism (PE).DVT refers to abnormal blood clotting in the veins and impedes venous return.The dislodgement of emboli from the vessel wall can form a thrombotic embolism,and cause PE,myocardial infarction,and stroke; it can not only prolong hospitalization,but also increase the mortality.This article reviews the incidence,risk factors,treatment,and prevention of DVT after a stroke.

Glutathione S-transferases (GSTs) are large super-gene family.It is traditionally thought to be a kind of electrophilic detoxification substance.However,recent research showed that it has a powerful antioxidant role,but its gene polymorphism is also a risk factor of variety diseases.Researches verified that GSTs are widely found in the epithelial tissue in human and eye tissues,such as iris,ciliary,trabecular meshwork,lens,macula and retina.GSTs play strong preventive and treating roles for many oxidative-induced eye diseases,including primary open-angle glaucoma ( POAG),age-related cataract and age-related macular degeneration ( AMD ).The antioxidative mechanism of GSTs to eye diseases is in study.The current researches about the element concept,distributions in eye tissues,gene polymorphism of GSTs and its relationship with eye oxidative damage were reviewed.

Biosafety cabinets(BSC)is the primary containment barrier.It is very important to choose suitable control method for the protection ability of biosafety cabinet.This paper briefly introduces the key points and several control methods as well as the different characteristics of BSC control.

Objective To study the relationship of single nucleotide polymorphism of angiotensin converting enzyme 2 ( ACE2 ) with essential hypertension(EH) and antihypertensive response of valsartan.Methods A total of 120 case EH patients was received valsartan once daily for four weeks.Direct DNA sequencing was performed to detect the signgle nucleotide polymorphisms of ACE2 in 120 EH patients and 60 controls with normal blood pressure.ResultsThe genotyping data indicated that there were significant differences of G allele frequency between male or female EH group and controls( x2 =5.310,4.423,P ＜0.05 ).Treated with valsartan,patients with.G allele were not found to be associated with reduction in blood pressure( P ＞0.05).Conclusions Our data suggest that the ACE2 G8790A signgle nucleotide polymorphisms might be involved in the development of EH.The risk developing hypertension in the people carrying G allele is higher than that in those carrying other allele.Therefore,ACE2 gene might be a sensitive gene associated with EH.

The ubiquitin-proteasome system(UPS) containing ubiquitin-activating enzyme(E1),ubuquitin-conjugating enzyme(E2s),ubiquitin-ligase(E3s) and 26S proteasome,is involved in the degradation of most intracellular proteins.One major function of UPS is to prevent accumulation of non-functional and potentially toxic proteins.Moreover,it has become clear that the UPS fulfills an important function in most aspects of eukaryotic biology,such as inflammation,cell proliferation,intracellular signaling,transcriptional control,and apoptosis.Recent studies demonstrated that the UPS regulates the initiation and progression of atherosclerosis,ischemia-reperfusion injury,familial cardiomyopathies,hypertrophic and heart failure.This review briefly summarizes current knowledge about structure,function,regulation of UPS and its pathophysiological role in cardiovascular diseases.

Buthionine salfoximine (BSO) is a chemotherapeutic sensitizor under clinical trial. BSO can reverse multidrug resistance in vitro and in vivo. The author reviewed the mechanism, pharmacodynamics, pharmacokinetics and the progress in clinical research of BSO. BSO is a selective inhibitor of 7-glutamylcysteine synthetase, the rate-limiting enzyme in gluthionine synthesis. Depletion of intracellular gluthionine by BSO can reverse tumor resistance to chemotherapeutic a-gents such as platinating and alkylating agents. In tumor-bearing animals BSO enhanced the therapeutic index of chemotherapeutic agents. Animals treated with BSO had a longer life span. Pharmacokinetics of BSO was a two-compartment manner in mice, dogs and huamn beings. The efficacy of BSO in cancer patients is being investigated in clinical trial.

Objective:To establish an indirect immunofluorescence assay for detection of serum IgG antibody against HHV-6,and to detected antibodies of HHV-6 in Changchun city.Methods:Cord blood mononuclear cells infected by HHV-6 strain GS were used to prepare cell antigen slide so as to establish an IFA and was used detect the HHV-6 antibody level in serum for Changchun city population.Results:An indirect immunofluorescence assay was established for detection of serum IgG antibody against HHV-6,at the same time the HHV-6 antibody level in serum for Changchun city population have been detected,and positive rate of HHV-6 antibody was 65.2%.Conclusion:A specific IFA has been established successfully for the investigation of HHV-6 infection.