Objective To study the pharmacodynamics features of cisatracurium besylate in differ-ent age patients. Methods One hundred and eighty patients were randomized to group Ⅰ (50-60 years old),group Ⅱ (61-70 years old)and group Ⅲ (above 70 years old).Each group was subdivided according to the dose of cisatracurium besylate, 0.10 mg/kg (2ED95)or 0.15 mg/kg (3ED95) into group Ⅰ2, Ⅰ3, Ⅱ2, Ⅱ3,Ⅲ2, Ⅲ3(n = 30).ECG, SpO2, PETCO2, HR and neuromuscular blockade were monitored. Intubation conditions were assessed when T1 was zero after cisatracurium besylate administer ration using a 4-part scale.The onset time, TOF no reaction time, duration time, recovery lime and hemodynamic response time were recorded. Re-sults The onset times of group Ⅰ3, Ⅱ3 and Ⅲ3 were significantly shorter than those of group Ⅰ2, Ⅱ2 and Ⅲ2 (P < 0.05).The percentages of patients with excellent intubation condition in group Ⅰ3, Ⅱ3 and Ⅲ3 were 93%, 90%, and 80%, which were markedly higher than those in group Ⅰ2, Ⅱ2 and Ⅲ2 (67%, 60%, 60%). TOF no reaction time and duration time were longer in group Ⅰ3, Ⅱ3 and Ⅲ3 than those in group Ⅰ2, Ⅱ2 and Ⅲ2. There was no significant difference in muscle chalasis among the six groups. Conclusions Cisatracuri-um besylate 0.15 mg/kg produces quicker onset time and acceptable intubation conditions in the different age patients than 0.10 mg/kg. Recovery time is independent on the dosage and age. Aging has no influence on the phannacodynamics of single injection of cisatracurium besylate.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Arrhythmias, Cardiac ; therapy ; Female ; Humans ; Male ; Middle Aged ; Needles

Objective To investigate the effect of small interfering RNA(siRNA)-expressing plasmid targeting connective tissue growth factor(CTGF) on the expressions of its mRNA and protein in cultured human keloid fibroblasts(hKFs).Methods Constructed siRNA-expressing plasmids targeting CTGF were transfected into hKFs by Dosper package.Then the transfected cells were treated with transforming growth factor 1(TGF-?1,10 ng/ml).Normal hKFs cells and those with TGF-?1 treatment served as normal control and control groups respectively.The expression level of CTGF mRNA and protein were detected by real-time fluorescent quantitative PCR(RQ-PCR) and Western blotting respectively.Results The expression level of CTGF mRNA was only 56.6% of that of the normal control(P

Objective To study the effects of the expression of hypoxia inducible factor-1 alpha (HIF-1?) induced by FasL on the invasiveness of rectal cancer cells. Methods With molecular cloning method, eukaryotic expression vectors of pcDNA3.1 FasL containing total length of FasL cDNA sequence were transfected into human rectal cancer cells HR-8348. The cell invasiveness was examined. A hypoxia model of HR-8348 was reproduced, and its invasiveness was assessed with transwell methodology. Expression of HIF-1? was assayed by Western bloting. Results The number of HR-8348 cells transfected with FasL penetrated transwell filter membrane was larger (12.930?2.434) than that of empty vector transfected cells (7.670?2.093) and control cells (8.133?1.959) with statistically significant differences (P0.05), but it was significantly higher in FasL positive HR-8348 cells than in FasL negative cells at 12h and 24h after hypoxia (P0.05). Conclusions FasL is an effective factor inducing HIF-1? expression, and FasL expression correlates positively with HIF-1? expression. Enhanced FasL expression in rectal cancer cells could induce higher expression of HIF-1?, enable the tumor cells to adapt to hypoxia environment, and enhance the invasiveness of tumor cells.

Objective To explore the levels of serum angiotensin Ⅱand their clinical significance on pheochromocytomas. Methods Fifty-eight patients were randomly divided into three groups: Group Ⅰwith normal blood pressure; Group Ⅱ are essential hypertension; Group Ⅲ are pheochromocytomas. The levels of serum angiotensin Ⅱ (ATⅡ) in each group and at eleven time points: were measured. Results The levels of serum ATⅡ of groupⅡand Ⅲ were significantly higher than those of groupⅠ(P

Objective　To observe the effect of mycophenolate mofetil (MMF),a new type of immunosuppressant,on the immune function in patiens with systemic lupus erythematosus (SLE).Methods　The changes of serum lL-6,IL-8,TNF-α,sIL-2,ANA,A-dsDNA and subsets of lymphocytes were observed in patients with SLE before and after treatment with MMF and CTX by ELISA,indirect immunofluorescent assay and flow cytometry,and the effect of MMF and CTX on the functions of hematopoiesis,liver and kidney were also observed.Results　Three months after treatment with MMF,the serum levels of IL-6,IL-8,TNF-α,sIL-2,ANA,and A-dsDNA in patients with SLE were significantly decreased,CD3＋,CD4＋ and CD4＋CD45RA＋ cells were significantly increased,and CD8＋,CD4＋CD45RO＋,CD8＋CD45RA＋,CD8＋CD45RO＋ cells were significantly decreased.No remarkable injury was observed on the functions of hematopoiesis, liver and kidney after treatment with MMF.Conclusion　MMF can inhibit T and B lymphocytes selectvely and is a new type of immunosuppresant to treat SLE with less toxicity and side-effect.

Objective To evaluate the effect of biofeedback therapy for the treatment of myogenic fecal incontinence. MethodsA general assessment about anorectal function was made on 17 cases receiving biofeedback therapy including muscle power training,sensory training and coordination training. Results The clinical scores before and after biofeedback therapy were 1.66?0.23,3.80?0.42 respectively,with an effective rate of 82%. The anus maximum contracting pressure elevated,(73?20) mm Hg vs. (123?30) mm Hg; myoelectric amplitude increased,(122?32) ?V vs. (230?41) ?V;Contracting time prolonged,(4.1?2.0) s vs. (9.4?3.0) s; The sensory threshold was lowered,(50?12) ml vs. ( 20? 10) ml;The feel-contract time increased,(3.1?0.4) s vs. (1.2?0.3) s. Positive rectal contraction reflex was seen in 41% patients before therapy compared with 82% after therapy. Conclusions Biofeedback therapy increases contractility of sphincter,decreases threshold of rectal sensory,and is a therapy of choice for myogenic fecal incontinence.

Objective To investigate the effects of Huangkui Capsules on oxidative stress and micro-inflammation state in diabetic nephropathy patients with maintenance hemodialysis. Methods Forty-six patients with diabetic nephropathy who had received maintenance hemodialysis for over six month in Gansu Provincial People's Hospital were randomly divided into observation group (23 cases) and treatment group (23 cases) from January to December in 2014. Twenty healthy subjects who had physical examinations were selected as the control group at the same time. Observation group were given conventional hemodialysis for 4 hours, 3 times a week; treatment group were given conventional hemodialysis combined with Huangkui Capsules, orally, 2.5 g each time, 3 times a day. Both groups were treated for 8 weeks. Blood was collected before and after treatment for detection of hypersensitive C-reactive protein (hs-CRP), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), white blood cell (WBC), malondialdehyde (MDA), cycle of advanced oxidation protein products (AOPP), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels. Results The levels of IL-6, TNF-α, AOPP, WBC, MDA and hs-CRP were significantly higher in the observation group and the treatment group compared with the control group before treatment (P<0.01), SOD and GSH-Px levels decreased (P<0.01); there was no statistical significance between the observation group and the treatment group (P>0.05). After treatment, the levels of IL-6, hs-CRP, MDA and AOPP increased in the observation group (P<0.05), GSH-Px and SOD decreased (P<0.05), there were no statistical significance in TNF-αand WBC (P>0.05);The levels of AOPP, TNF-α, hs-CRP, WBC, MDA and IL-6 in treatment group significantly decreased (P<0.01), GSH-Px and SOD significantly increased (P<0.01); there was statistical significance between the observation group and the treatment group (P<0.01). Conclusion Micro-inflammation state and oxidative stress exist generally in diabetic nephropathy maintenance hemodialysis patients. Huangkui Capsules can improve oxidative stress and micro-inflammation state in diabetic nephropathy maintenance hemodialysis patients.

Objective To explore the expression and clinical significance of IL-12 in acute cardiac allograft rejection in rats.Methods Two groups of SD rats received cervical heterotopic heart transplantation from allogeneic Wistar or syngeneic SD rats.The cardiac grafts were harvested at 1,3,5,and 7 days after transplantation and detected for the expression of IL-12 mRNA by reverse transcriptional polymerase chain reaction(RT-PCR).Results With the posttransplantation day delayed,the expression of IL-12 mRNA was significantly increased in allogeneic transplant group(P

The pharmacokinetics of ketamine were studied in 8 preschool children,aged 3.5～6 years. The ketamine was administrated by I. V. infusion at a dose of 3mg?kg~(-1) in one hour constantly. The plasma concentrations of ketamine were measured by high performance liquid chromatography. The disposition of intravenous infusion with ketamine could be described by two-compartment open model, and the variables of pharmacokinetics of ketamine were as follows: T_(1/2?)=0.15?0.04 hours,T_(1/2?)=2.84?0.44 hours,Cl=0.34?0.06L?kg~(-1)?h~(-1), AUC=9.21?1.00mg/L?h, and V_d=0.66?0.29 L?kg~(-1). This suggests that intravenous infusion of ketamine at 3mg?kg~(-1)?h~(-1) is safe for the maintenance of general anesthesia in preschool children, attention must he paid to the children anesthetized with long period of ketamine infusion because recovery may be delayed due to the prolonged elimination half-life.