BACKGROUND:With the in-depth study of cancer stem cels, increasing evidence has shown that cancer stem cels are the reason for cancer metastasis and recurrence, and therefore it is of great significance to isolate and identify cancer stem cels that can elucidate the pathogenesis of cancer and development of anticancer drugs.
OBJECTIVE: To isolate and culture gastric cancer stem cels and then to detect its biological characteristics.
METHODS: Cancer tissues from 16 patients with gastric cancer were cultured using adherent method and enzymic digestion methods to isolate gastric cancer stem cels. The cel morphology was observed under inverted microscope to drawn out growth curves. Osteogenic and adipogenic ability of cels were also detected.
RESULTS AND CONCLUSION: Gastric cancer stem cels were isolated successfuly by both two methods. Under the microscope, the cels were fusiform- or polygon-shaped. At confluence, the cels grew in a vortex or radial manner. The cel growth curve showed that 1-3 days were latent period, 4-9 days were logarithmic growth phase, and over 10 days were growth plateau phase. Flow cytometry results showed that passage 3 gastric cancer stem cels highly expressed CD90, CD29, CD44, but lowly expressed CD34, CD45 and HLA-DR. After osteogenic induction, calcified nodules were clearly visible in the gastric cancer stem cels at passage 3; after adipogenic induction, bright and tiny fat droplets were seen in the cytoplasm. These findings indicate that cancer stem cels exist in the gastric cancer tissues, and have similar morphology, biological property and multipotent differentiation ability to normal cels, which are probably involved in gastric cancer occurrence and development.

Bilateral vasovasostomy was performed on 14 male rabbits which received a vasectomy 12 months ago. After 3 months, the rabbits were mated with female rabbits in a course of two months in order to observe the restoration of fertility. They were divided into vasovasostomy fertility group(VFG)and vasovasostomy infertility group(VIG), 7 rabbits, each according to their fertility. 8 rabbits that received a vasectomy only were designated as vasectomy group(VG), and 9 as sham-operation group(SOG), both (VG & SG) served as control. The results showed, (1)the value of sperm density in VFG was significantly lower than that in SOG, but markedly higher than that in VIG(P

AIM:To analyze the 34 cases of patients with diagnosis of lacrimal duct disease, to explore the cause and clinical characteristics of lacrimal duct inflammation, and to study the common misdiagnosis to improve the diagnosis rate, to provide clinical experience for medical workers.
METHODS: A total of 34 eyes of 34 patients were selected from 2011-06 to 2015-06 to our hospital for lacrimal duct incision. Clinical data of patients including age and gender distribution, eye and lesion location, duration, canaliculitis clinical symptoms and previous misdiagnosis were collected. The operation of lacrimal duct was carried out, and the secretion of lacrimal secretion was checked.
RESULTS:Patients over the age of 40 accounted for 77%of the population, men accounted for 23. 5%, women accounted for 76. 5%. Left eye in 15 cases, right eye in 19 cases, and were monocular onset, 26 cases ( 76%) of patients with lower lacrimal duct inflammation. Clinical symptoms: increased secretion, canthal conjunctival congestion, swelling, lesion location around the lacrimal puncta swelling and tenderness, slightly raised, epiphora, foreign body sensation. The culture results Showed 34 cases of discharge inspection. A total of 20 cases were fungal growth, 6 cases of bacterial growth rate were 59%, 18%, and 8 cases were not detected, 10 cases of lacrimal duct stone were found by pathological examination. There were 3 cases of Aspergillus, 5 cases of Candida, 2 cases of actinomycetes, 4 cases found in Gram staining microscope. Misdiagnosis of canaliculitis for other eye diseases, including 12 cases of chronic dacryocystitis, 8 cases of chronic conjunctivitis, 6 cases of acute conjunctivitis, stye in 4 cases, the misdiagnosis rate reached 88%.
CONCLUSION:In the middle and old aged people, the lacrimal duct is more than that of the male. The main pathogenic bacteria causing lacrimal duct infection may be fungi, which can be treated with anti-fungal drugs. For the removal of the lacrimal duct stone, we should distinguish the types of pathogenic bacteria for the treatment of the disease. In the case of misdiagnosis, the clinical manifestations of different clinical manifestations from the lacrimal duct infection and other diseases of the eye should be carefully examined and carefully treated.

OBJECTIVE:To uncover the potentiality of brand management in pharmaceutical industry and to explore its importance in pharmaceutical industry.METHODS:The developmental opportunity of brand management was analyzed be?ginning from the current circumstances of pharmaceutical industry.RESULTS&CONCLUSION:The pharmaceutical in?dustries are confronted with unprecedented development for brand management.With the ending of the products era,brand management has become an important resource for the pharmaceutical industry to obtain competitive advantages.

OBJECTIVE: To provide referrences for the state drug administrations strengthening supervision and administration, and also for the pharmaceutical enterprises develeping drug marketing.METHODS: To explain the concept of DTC marketing.And the present situation of drug DTC marketing in several foreign countries were discussed and its development process and existing problems in these countries were also analyzed. RESULTS: Drug DTC marketing was under development in the world and pharmaceutical enterprises hawe showed increasing interest in it, however, drug DTC marketing needed further standardization and improvement. CONCLUSION: Drug DTC marketing holds a promising prospect, the effective and standardized development of it will be a big challenge to state drug administrations and pharmaceutical companies in the world.

To study the underlying mechanism of vasovasotomy infertility, we set up the rabbit model and observed the levels of testosterone, IL-1 activity and TNFα, which represent the functions of monocytes and macrophages on the animal model of vasovasostomy, and probed into the in vitro effect of IL-1β on the production of testosterone by Leydig cells. Rabbit model was set up and divided into vasovasostomy fertility group (VFG) and vasovasostomy infertility group (VIG), and set up peer group of Vasectomy group (VG) and sham operation group (SOG) as controls. The results were as follows: The sperm density and total number is highest in SOG, with that in VFG markedly lower than that in SOG, but significantly higher than that in VIG. 　　1. VFG showed significant higher level of testosterone (Ts) than SOG, VG and VIG; Ts of VIG was significantly lower than SOG or VFG. In the vasovasostomy groups, sperm density is positively correlated to the level of testosterone. 　　2. The level of serum cortisol in VIG significantly higher than that in VFG, SOG, and VG. The level of serum cortisol negatively correlated to serum testosterone. 3. Serum estradiol(E2) was similar among all the groups (P＞0.05). E2/T ratio is significantly different among the groups, with that in VIG highest and that in VFG lowest. 4. Serum IL-1 activity in VIG was significantly higher than that in SOG and VFG(P＜0.05). 　　5. Serum TNFα and serum IL-1 activity share similar change, with TNFα in VG significantly higher than that in SOG(P＜0.01), VIG was the highest among the four groups(P＜0.01). Correlation test showed that serum IL-1 activity and TNFα level was negatively correlated to testosterone level; positively correlated to cortisol level and E2/T ratio. 6. Immuno-histo-chemistry of CD68 and TNFα in rabbits with vasovasostomy showed that in the testis tissue of SOG, interstitial tissue was normal with no filtration of inflammation cells including CD68 cells. In VFG and VIG, the tubules with resumed sperm production showed negative staining, and atrophic tubules and their surroundings were expression positive of CD68, with that markedly deeper in VIG than that in VFG. TNFα positive expressed granules was found only in atrophic convolted seminiferous tubules and their surroundings, with that deeper in VIG than that in VFG. 7. Culture Leydig cells were put in the following holes:(1) Control; (2) hCG; (3) IL-1; (4)IL-1 β+hCG; (5) IL-1 β+IL-1Ra; and (6) hCG+IL-1β+IL-1Ra. Determined the levels of testosterone by immuno-radiation technique. (1) IL-1 β showed no obvious effect on the Leydig cultured in vitro, but depressed the induction of Leydig cells by hCG to synthesize testosterone; (2) IL-1Ra blocked the depression of IL-1β Leydig cells' bio-synthesis of testosterone induced by hCG. In summary, one of the obvious changes in animals with vasovasotomy is low density of sperms, which is related to the lowering of testosterone. Low level of testosterone is related to the activation of monocytes and macrophages to produce IL-1 and TNFα, which negatively regulate the production of testosterone.

Objective To explore the resilience characteristics of the traumatic fracture patients and its influence on acute stress disorder(ASD),and provide the basis for improving the population's ability to cope with stressful events and resilience level for clinic.Methods 440 cases of traumatic fracture patients hospitalized in Tangshan Second Hospital were selected in this study.All subjects were assessed with SAS-RQ,CD-RISC,AIS-ISS,VAS and the general condition of investigations.Results The resilience level of traumatic fracture patients was at the middle,the resilience total score was(51.13±14.81) points,and lower than that of the national norm(t=-20.217,P＜0.01).Sex,age,personality and injury severity had the impact on resilience (P＜0.05).Resilience dimensions and total scores had negative correlation with SASRQ dimen sions and its total score (r=-0.587--0.739,P＜0.01).High-leveled resilience patients have lower ASD scores and lighter symptoms(P＜ 0.05).The multivariate analysis appeaed that resilience and the degree of pain were the main factors affecting ASD (P＜ 0.05).Conclusion The resilience level of the traumatic frac ture patients should be further improved and the construction of resilience should be strengthened in order to prevent the occurrence of ASD.

Objective To investigate the evaluation value of spatiotemporal image correlation (STIC) on fetal cardiac systolic function .Methods The fetal cardiac cycle images were collected by the four-dimensional(4D) STIC ultrasound technique in 160 normal singleton pregnancies .The virtual organ computer-aided analysis(VOCAL) technique was used to measure the ventricular end-systolic volume (VESV) and ventricular end -diastolic volumes(VEDV) .The stroke volume(SV) ,ejection fraction(EF) and cardiac output(CO) were calculated .Results VESV ,VEDV together with SV and CO in normal pregnancy were increased with gestation week increase .EF remained constant with advancing gestational week .VESV ,VEDV ,SV ,CO and EF of right ventricle were all larger than those of the left ventricle .Conclusion The fetal cardiac systolic function in normal pregnancy is increased with gestational age increase ,the right heart systolic function is superior to the left heart .

AIM: To compare the clinical efficacy and safety of phacoemulsification and small incision non -phacoemulsification cataract surgery, and provide better options for clinical cataract treatment.
METHODS: According to the different operation methods, 98 cases of simple senile cataract patients in our hospital were divided into control group and treatment group, 49 cases in each. The control group received ultrasonic emulsification operation treatment; treatment group were treated by small incision non -phacoemulsification. Visual acuity, astigmatism values, average operation time, and complications were compared between two groups before and after operation.
RESULTS: There was no significant difference in preoperative corneal astigmatism values of two groups at 3mo between two groups (P>0. 05). On other times, vision and corneal astigmatism were obviously better than those before operation (P<0. 05). The average vision, corneal astigmatism values and complications incidence of two group at operation time and different postoperative time had no statistical difference (P>0. 05). When the lens nucleus hardness was at Ⅰ~Ⅲ level, corneal endothelial cell count of two groups had no significant difference ( P>0.05). When the lens nucleus hardness was at Ⅳ ~ Ⅴlevel, there was statistical difference (P<0. 05).
CONCLUSION: Small incision non-phacoemulsification cataract surgery has the similarly efficacy compared with phacoemulsification. It should be based on the actual situation of the hardness of nuclear to select the appropriate surgical treatment.

Objective To explore the clinical and laboratory characteristics of langerhans cell histiocytosis (LCH) in children, so as to improve diagnosis level and decrease misdiagnosis rate.Methods Twenty-five cases of LCH from Jan.1996 to Feb.2006 were analyzed by retrospective study. The clinical data were collected and abstracted for information regarding clinical symptom, physical sign, laboratory examination, imaging,pathology,diagnosis and treatment.Results Some laboratory findings in hemogram, bone marrow examination and chest X-ray were non-specific.The X-ray characteristic of skeleton was osteolysis. Computerized tomography and magnetic resonance imaging were important in defining the extent of lesion in fundus cranii and sella.Seven cases were examined for anti-Epstein-Barr virus(EBV) IgM, 3 cases were positive;5 and 3 cases out of 10 cases showed humoral and cellular immunity abnormality,respectively. The misdiagnosis rate was 52%,1 case had been misdiagnosed for 7 years. Chemotherapy was effective in the future.Conclusions The clinical manifestations of LCH varies widely, leading to high rate of misdiagnosis.The etiology of LCH is unclear,and some of our patients show the evidence of EBV infection or immunity abnormality. Definitive diagnosis of LCH is based on pathology. Ultrastructure and immunophenotype should be done to improve diagnosis level.