The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

s:Primary chronic intestinal pseudo-obstruction (PCIPO)is a rare digestive syndrome characterized by derangement of gut propulsive motility which resembles mechanical obstruction, in the absence of any obstructive process. Treatment of intestinal pseudo-obstruction involves nutritional, pharmacological and surgical therapies, but it is often unsatisfactory and the long-term outcome is generally poor in the majority of cases. This article is aimed at reviewing the current knowledge on etiology, clinical features and management of patients affected by PCIPO.

Long non-coding RNA was initially identified as "noises" of gene transcriptions. However, with the developing researches of ENCODE, it was found that the long non-coding RNAs can regulate the genomic expressions in the form of RNAs in epigenetic, transcription, and post transcriptional levels, which is involved in the regulation of diverse cellular processes and has significant influences on occurrence and precaution of human diseases. This paper introduces functions and features of the long non-coding RNAs, and sums up the internal relation between long non-coding RNAs, diabetes and diabetic complications on the basis of existing researches. These advances can provide the basis for the further understanding of molecular medicine on occurrence and evolution of diabetes.
Diabetes Complications ; genetics ; Diabetes Mellitus ; genetics ; Humans ; RNA, Long Noncoding ; genetics

Objective To set up the filter mating method of plasmid conjugation experiment for Corynebacterium.Methods The first plasmid conjugation between different genus was performed by filter method.Three strains of highly erythromycin-resistance Enterococcus faecalis isolates[minimal inhibitory concentration(MIC)of erythromycin>256 mg/L,MIC of levofloxacin≤8 mg/L] which screened by agar dilution method were used as donators and 3 strains of Corynebacteria isolates(MIC of erythromycin≤32mg/L,MIC of levofloxacin=128 mg/L)were used as acceptors.In the second conjugation between the same genus but in different species,three Corynebacterium xerosis strains(MIC of erythromycin>256 mg/L,MIC of ceftazidime=16 mg/L)which were conjugants of the first conjugation were used as donators and 6 strains of Corynebacteria isolates(MIC of erythromycin≤32 mg/L,MIC of ceftazidime 128 mg/L)were used as acceptors.Results In the first conjugation between different genus,the plasimid conjugations were done for 9 times between 3 donators and 3 acceptors,4 of which gained the phenotype and genotype of resistance and the transfer rate was 44%.In the second conjugation experiment,the plasmid conjugations were done for 18 times between 3 donators and 6 acceptors,7 of which gained the resistant phenotype and the transfer rate was 39%.Erythromycin-resistance was transferred from the donators to the conjugants in varying degrees.The resistance was transferred from Enterococcus faecalis to Corynebacteria and then to other Corynebacteria which were of the salne genus but of different species.Conclusion Filter mating conjugation method could be used to study the mechanisms of resistance transmission of Corynebacterium.

Objective To study the clinical analysis in the neonatal hypoxic-ischemic encephalopathy (HIE)for evealuation of the severity of brain injury and prognosis. Methods The manifestation,laboratory findings,outcomes of 117 HIE were analyzed. Results Manifestations of HIE in 117 infants included 3 groups(minor level,moderate level,severe level). There was no significant difference in the weight and gestation-age among 3 groups.Scores of Apgar lowing,which Was marked in the severe level(P＜0.01). There was significant difference in the level of pH and PaCO2(P＜0.01). There was significant difference in the level of blood GLU and BE reduced aboviusly in the severe level,and in the level of BUN,CK-MB,CK increased aboviusly in the moderate and severe level,among all(P＜0.01). 69 patients were taked CT on the brain,the abnormal rate of which was 82.6%. The abnormal rate of CT on the brain increased 100% changing with the severity of brain injury on HIE especially in the moderate and severe level. Conclusion The prognosis of the minor level of brain injury on HIE is the best. The prognosis of the moderate-level group who was treated reasonably and enough,is better mostly.

Xenogeneic/Allogeneic bone materials are among the hot research interests of biological scaffolds.These materials are ideal materials for repairing bone defects because they have the structure and morphology of the natural bones,are able to be degraded and absorbed in the body,and are less immunogenic through appropriato treatment.Through further modification with biological molecules such as growth factors,they could provide a better microenvironment for the bone cells to grow into and fully differentiate.This article summarizes the factors affecting the immunogenicity of xenogeneic/allogeneic bone and the methods of removing immunogenicity.Advances in sintered bone materials,defatted bone materials,deproteinated bone materials,decalcifying bone materials,and composite materials are reviewed in greater details.

Objective To investigate the application of numerical simulation technique in supraorbital and frontal osseous defect.Methods The data of the patients with complex supraorbital and frontal osseous defects were obtained by helix CT scan.Numerical model based on the data was built up by computer.And the true model was produced by Rapid Prototyping.Being stimulated before this operation.the model was directly used in the operation.Results All of these 12 patients(one of defect of brow,four fracture of brow and zygoma,seven of old fracture of brow and zygoma)in this study were satisfied and their brows were symmetry after operation.All of them were satisfied after 1 or 2 years,without implantation exposure and transfiguration except one with infection.Conclusion Based on numerical technique difficulties of repairing,complex supraorbital and frontal osseous defect could be resolved by such process as collecting the bone data,stimulating operation by true models,and producing implantation before the operation.The accuracy of these operations can be improved.

Objective To discuss the changes of quality of life and influence factors in patients with multidrugresistant pulmonary tuber-culosis. Methods Using the SF-36 scale of 80 normal subjects and 80 cases of MDRTB patients before and after treatment and quality of life score total score evaluated dimensions. Results Between the patients with MDR-TB and the control in the total Qol scores and eight dimensionality, there were significant differences(P＜0.01), total Qol scores and eight dimensionality in 3 months or 1 year after treatment were higher than before therapy,there were significant differences(P＜0.01 ). Between 1 year after therapy in the total Qol scores and PF, PR, GH, VT, SF, ER, MH, dimensionality were lower than control groups, there were significant differences(P＜0.05 ). The main factors affecting the Qol of patients with MDR-TB were cultural level, kinds of drug resistant and reduction of counts of white blood cell. Conclusion The correlation between quality of life and education, the number of drug resistance, reduction of white blood cell in patients with multi-drugresistant pulmonary tuberculosis.