Objective To develop a method on evaluating the uncertainty during the determination of cefixime in human plasma by high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/ MS). Methods We analyzed various factors that can cause the uncertainty in the whole process of determination, which includ repeatability, weighting,preparation of standard solutions, sample preparation, equipment error and calibration curve fitting. The expanded uncertainty was evaluated by calculating uncertainty of each component and combined. Results The expanded uncertainty for low ( 80 ng ? mL-1 ), medium ( 560 ng ? mL-1 ) and high ( 9 600 ng ? mL-1 ) level of cefixime was 14. 93, 23. 65, 137.95 ng?mL-1 ,respectively (P= 95%). Conclusion The uncertainty of determining cefixime in human plasma by HPLC-MS/ MS is mainly caused by the calibration curve fitting,repeatability and HPLC-MS/ MS error.

Objective To explore the relationship of adiponectin and brachial ankle pulse wave velocity (baPWV) in type 2 diabetes mellitus patients combined with hypertension.Methods Fifty-six patients with diabetes mellitus,69 diabetes mellitus patients combined with hypertension and 78 healthy people were recruited in our study who visited doctors in Fenyang Hospital from March 2010 to March 2012.The levels of adiponectin,brachial ankle pulse wave velocity and other biochemical indicator were determined.Analyzed the correlation between adiponectin and baPWV among the three groups.Results The levels of adiponectin in control group,diabetes mellitus group,and diabetes mellitus combined with hypertension group were (13.9 ±4.2) mg/L,(9.1 ± 3.8) mg/L and (7.6 ± 2.4) mg/L respectively,and the difference was statistically significant (F =62.53,P ＜0.01).The levels of baPWV in control group,diabetes mellitus group and diabetes mellitus combined with hypertension group were (1 617.4 ± 198.2) cm/s,(2 010.9 ±231.7) cm/s and (2 196.1 ±218.6) cm/s respectively,and the difference was also statistically significant (F =139.41,P ＜ 0.01).The correlation between adiponectin level and baPWV among three groups were-0.629,-0.561,and-0.528,respectively (P =0.027,0.034,0.029).Conclusion The levels of adiponectin decreased in diabetes mellitus patients combined with hypertension,while the baPWV increased.There is a good correlation between adiponectin and baPWV.

[Summary] Following shoulder surgery , proper regional anesthesia is usually required to minimize the use of opioids .Regional anesthesia techniques commonly used include subacromial or intra-articular local anesthetic infiltration , suprascapular nerve block with or without axillary nerve block , and interscalene block .This paper summarized literatures on the three abovementioned techniques in respect with performance , merits and flaws , and future perspectives .

MicroRNA (miRNA)is closely related to the occurrence and development of neoplasms. miR-139 is a tumor-suppressive miRNA and its expression is decreased in a variety of tumor tissues,while over-expression of miR-139 can inhibit cancer cell proliferation,migration,invasion and induce cell apoptosis by suppressing the expression of multiple kinds of target genes.Therefore,miR-139 has potential applications in tumor diagnosis,treatment and prognosis assessment.

Objective To observe the clinical efficacy ofXiaozhikang Formula Granules for the treatment of nonalcoholic fatty liver disease.Methods Totally 90 patients with nonalcoholic fatty liver disease were randomly divided into control group and treatment group, 45 cases in each group. Patients in the control group were treated with conventional western treatment and oral metformin enteric-coated metformin hydrochloride, while patients in the treatment group were treated withXiaozhikang Formula Granules on the basis of conventional western treatment for 3 months. Blood lipid and insulin resistance in the two groups before and after treatment were observed, and the clinical efficacy between the two groups was compared.Results Clinical excellence (35.5%), total effective rate (84.5%) and TCM syndrome score (13.78 ± 3.6) after treatment of treatment group were better than those of control group (P<0.05). GPT, GOT, TG, and TC in both groups were lower after the treatment (P<0.05,P<0.01). There existed statistical significance in TC and TG between the two groups after treatment (P<0.05). There existed statistical significance in B ultrasound index between the two groups after treatment (P<0.05), and the treatment group was superior. FBG and HOMA-IR in both groups significantly decreased after treatment (P<0.05), and the FBG in the treatment group after treatment was better than control group (P<0.05).Conclusion The efficacy ofXiaozhikang Formula Granules in the treatment of nonalcoholic fatty liver disease is remarkable.

Human milk is the most suitable food for newborns,and is greatly beneficial to the health and growth of infants.In some circumstances,human milk need cold storage,but freezing/cooling storage will inevitably cause the loss of components in human milk.The most reasonable storage condition of human milk has not reached an agreement.This article focuses on the change of macronutrients,immune substance and microbial indicators of human milk which had been cold storaged,and summarizes the related researchs of home and abroad in recent years.

Objective To investigate the effects of acute nicotine exposure on expression of Caspase-3 and apoptosis of neuron in adolescent rat's brain. Methods The Caspase-3 expression was detected with immunohistochemistry and Western blotting. Apoptosis cells were detected by TUNEL method. Results The Caspase-3 expression in cerebral cortex, hippocampal dentate gyrus, CA2 and CA3 of the experimental group was higher than that of the control group through immunohistiochemistry. The expression of Caspase-3 prototype and active fragments in cerebral cortex and hippocampus of the experimental group was higher than that of the control group with Western blotting. The TUNEL positive cells in cerebral cortex and hippocampal dentate gyrus of the experimental group were more than that of the control group.Conclusion Acute nicotine exposure can activate Caspase-3 and increase neuron apoptosis in cerebral cortex and hippocampal dentate gyrus of the adolescent rat's brain.

BACKGROUND: The psychological researches on undergraduates popularly believe that status of mental health of undergraduates can be worried.OBJECTIVE: To investigate the relationship between personality and mental health of undergraduates in Henan province, and analyze the feasible value of developing mental health education effectively.DESIGN: Sampling investigation.SETTING: Department of Health Administration, Henan Medical College for Staff and WorkersPARTICIPANTS: 1 128 students from 3 university and colleges in Henan province, whose majors were agriculture, medical science, sciences,literal arts, arts and physical education, etc., were enrolled randomly in this study between April and June 2001.METHODS: Group test was performed in 1 128 students from higher learning schools of Henan province test with Eysenck Personality Questionnaire (EPQ) [including 4 dimensions: Extraversion (E), Neuroticism (N),Psychoticism (P), and Social Desirability (L)] and Symptom Checklist90(SCL-90) (90 items, 9 dimensions).MAIN OUTCOME MEASURES: ① Comparison of personality characteristics between undergraduates and national norms. ② Classified comparison of personality characteristics of undergraduates. ③ Relationship between personality characteristics and mental health of undergraduates.RESULTS: Incomplete questionnaires were excluded, and 1 100 qualified questionnaires were collected. ① Comparison of personality between undergraduates and national norms: Scores on N and L of EPQ in male undergraduates were higher significantly than those of the national norms (5.47 ±3.13,4.53 ±3.11 ;4.99 ±2.47,4.50 ±2.59,P ＜ 0.001 ).Scores on N, P and L of EPQ in female undergraduates were all higher remarkably than those of the national norms (2.97±1.71,2.68±1.82;5.68±3.08,4.97±2.96;5.32±2.46,4.74±2.48,P ＜ 0.05-0.001). ②Classified comparison of personality in undergraduates: There were obvious differences on the 4 dimensions of EPQ in students of 6 different specialties (P ＜ 0.05-0.001 ). Scores on P in agricultural students were the highest (3.44±1.97); Scores on E in students of physical education were the highest (8.27±2.45), while scores on N in literate arts students were highest (5.99±3.24). Male students'scores on L were lower markedly than female students'(4.99±2.47,5.32±2.46,P ＜ 0.05).Scores on E in students from cities were lower significantly than those in students from countrysides (7.54±2.73,7.93±2.63,P ＜ 0.05). Between lower grade (freshman year and sophomore year) and higher grade (junior year and senior year), only scores on L in the former were high er significantly than those in the latter (5.42±2.44,5.01±2.48,P ＜ 0.01 ).Moreover, scores on E in student cadres were higher apparently thanthose in non-student cadres (8.42±2.61,7.42±2.68,P ＜ 0.001). ③Relationship of personality and mental health in undergraduates: E and L of personality dimension in undergraduates had obviously negative correlation with every factor of SCL-90 (P ＜ 0.01-0.001 )while N had significant positive correlation with every factor of SCL90 (P ＜ 0.001),and P had significant positive correlation with somatization, hostility,phobic anxiety and paranoid ideation (P ＜ 0.05-0.01 ).CONCLUSION: Personality of undergraduates have extremely close internal correlation with their mental health. Introversion, emotional instability and psychoticism of personality of undergraduates have side effects on their psychological health. There are some differences of personality characteristics in different specialty, sex, grade, native place (city or countryside),cadre or non-cadre students.

0.05). But in repeated chemotherapy, the period of leukopenia recovered to normal was shorter in group B than that in group A or group C. The effects of the domestic rhG-CSF on leu-copenia induced by chemotherapy were similar to the imported products of same dose. CONCLUSION: Low dose of domestic rhG-CSF (75?g) can effectively treat the leukopenia caused by postchemotherapy in patients with gynecological tumors. 150?g of domestic rhG-CSF is more effective than 75?g of domestic or import rhG-CSF in treating leukopenia in repeated chemotherapy.

AIM: The pathogenesis of interstitial lung disease is still uncertain. This study observed the effect of interferon-gamma (IFN-?) in combination with methylprednisolone (M-pred) on the proliferation of human embryonic lung fibroblast (HELF) to identify whether it can be used as an effective treatment of interstitial lung disease in clinic. METHODS: The experiment was performed at the Research Laboratory of Cytobiology, Hebei Medical University from July 2005 to May 2006. ①The eighth passage HELFs were cultured in vitro. According to the common dose range for human of IFN-? and M-pred, cells were divided into control group, M-pred (15 mg/L) group, IFN-? (250, 500, 750, 1 000, 1 250 U/mL) groups and combination group (IFN-? and M-pred). ②HELFs were incubated with IFN-? alone or combined with M-pred for 48 hours before they were harvested. ③MTT assay was used to measure the inhibition rate of M-pred combined with different concentrations of IFN-? on HELF. Flow cytometry was used to observe cell cycle distribution and the proliferation index of HELF. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunocytochemistry. RESULTS: ①Both alone and combination usage of IFN-? and M-pred could inhibit HELF proliferation (P 0.85). ② IFN-? mainly acted on G1 and G2M periods, while M-pred on S period. The proliferation index decreased in combination group (P