Mesenchymal stem cells (MSC) is able to differentiate into a variety of epithelial cells,such as alveolar epithelium,tubular epithelium and corneal epithelial cells in vivo and in vitro under the given condition.After transfusion,MSC can effectively repair the injured lung epithelial cells,and relieve inflammation.MSC can also repair the renal tubular cell damage by reducing renal interstitial infiltration of inflammatory cells,promoting regeneration of renal tubular epithelial cells and inhibiting of apoptosis; Biological cornea can be available constructed by using MSC,which can availably repair the injured ocular surface.In the other hands,MSC can express keratinocyte growth factors and other cytokines,promoting limbal stem cell proliferation.In addition,MSC can also differentiate into pancreatic ductal epithelial cells,endometrial epithelial cells,spermatogenic epithelium and human retinal pigment epithelium.MSC has great application potential in epithelial diseases,and provide a good and effective choice in treatment of some epithelial diseases.

In order to improve the quality of humidification in patients with artificial airway during the mechanical ventilation, ensure the quality of artificial airway nursing. From the way of humidification , the choice of humidification liquid, the volume of humidification liquid control, the temperature of humidification liquid and the evaluation of humidification effect to research the present situation of humidification of artificial airway during mechanical ventilation. Put forward best way of patients with artificial airway nursing during the mechanical ventilation is wetting by temperature humidity of the Breathing machine′s and at the same time to strengthen the monitoring of the airway of humidification effect.

Laparoscope has been widely recognized as a treatment of distal gastric cancer.Totally laparoscopic distal gastrectomy and laparoscopy-assisted distal gastrectomy are two main types of laparoscopic distal gastrectomy.Apart from a smaller incision,some other advantages which totally laparoscopic distal gastrectomy has is the key point for further study.The thesis aims to compare totally laparoscopic distal gastrectomy to laparoscopy-assisted distal gastrectomy,and to analyze the characteristic in the aspect of operation time,the degree of pain,postoperative complications and the time of gastrointestinal recovery via collecting a great quantity of literatures.

Primary hypophysitis includes 3 subtypes:lymphocytic,granulomatous and xanthomatous hypophysitis. The primary hypophysitis has a low morbidity and the final diagnosis is mostly determined based on the pathological findings after operation.Now it is considered as an organ specific autoimmune disease.Its clinical manifestations and imaging features are similar to hypophyseal tumor,but the endocrine and pathologic features are different.Glucocortieoid pulse therapy and surgery are the two methods of treatment.

Objective To analyze the language structure of nursing records and the application of defined vocabulary in nursing records of community child care,and to explore the possibility of structuralization and standardization of community nursing records by researchers' consulting International Classification of Nursing Practice (ICNP(R)2.0).Methods Convenient sampling method was used.A total of 432 handbooks about child health care were extracted from three community health service centers in Liaoning province.By analyzing the nursing records,the vocabulary was selected,and a coding book was built.Then researchers compared them with ICNP(R)2.0 Chinese version.Results Totally 567 terms were extracted.All phrases in nursing records about community child health care can be classified into three categories and seven shafts,which were nursing phenomenon,nursing action and nursing outcome.Our community nursing records about child health care could not fully meet shaft level of nursing record writing requirements from the International Council of nurses.Only 146 records were labeled as perfect fit with ICNP(R)2.0 terminology,223 records as not fit at all.The difference of different levels of suitability distribution about nursing phenomenon,nursing action and nursing outcome had statistically significant difference.Words of able to adapt and can't adapt about nursing action had larger proportion.Words that can't adapt about nursing phenomenon and nursing outcome had larger proportion.Conclusions There is still certain gap between domestic nursing records and ICNP(R)2.0 terminology.The list of terms from comparison nursing records about community child health care with ICNP(R)2.0 can lay the foundation for nursing language system structure and perfect community nursing record language in our country.

Aim To explore the concentration range of organic solvent which can both effectively increase solubility of the difficult soluble medicine monomer, and have low toxicity to cells, and to clarify the influence of different concentration of ethanol on curcumol efficacy.Methods Different DMSO and ethanol concentrations were diluted in culture medium and incubated with cells A549, NCI-H460, NCI-H1299, NCI-1650, LTEP-a2 and SPC-A1 for 12 h, 24 h, or 48 h, cell viability was tested by a colorimetric assay with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT), and different concentrations of ethanol with/without different concentrations of curcumol were also prepared with culture medium, then incubate with A549 and NCI-H460 cells for 24 h, and cell viability was tested by MTT as well.Results Within 48 h the solution with 0.008(V/V) DMSO or less had no significant effect on the cell A549 compared with control group, for NCI-H1650 the concentration was 0.004(V/V) or less, for NCI-H460 the result turned to be 0.002(V/V) or less, and the solution with DMSO below 0.001(V/V) had significant effect on the three other cells, NCI-H1299, LTEP-a2 and SPC-A1.While within 48 h, the liquor with 0.004(V/V) ethanol or less did not exhibit significant cytotoxic effect on the cell A549, for NCI-H460 and NCI-H1650 the result of ethanol concentration became 0.002(V/V) or less, for NCI-H1299 the data was 0.001(V/V) or less, and the liquor with ethanol below 0.001(V/V) showed significant cytotoxic effect on LTEP-a2and SPC-A1.When the proportion of ethanol in solution was below 0.01, it has no cytotoxic on cell A549 and NCI-H460, and the curcumol solution prepared with this kind of ethanol solution only represented the efficacy of curcumol on the cells.Since the solution with 0.01(V/V) ethanol dissolved the curcumol better, the cell viability changed from about 100% to about 35%.Conclusions Different organic solvent expressed different toxicity to the same cell, and the sensitivity of different cells to one organic solvent is dissimilar.and DMSO could be the optimum solvent for A549 and NCI-H1650, while the optimum solvent of NCI-H1299 is ethanol, for NCI-H460 it could be both and DMSO and ethanol, and DMSO and ethanol is not suitable for LETP-a2, SPC-A1 to be solvent.

Anticonvulsants ; pharmacology ; therapeutic use ; Epilepsy ; drug therapy ; genetics ; Humans ; Pharmacogenetics

Objective: To understand the current status and characteristics of self-medication behavior in China and to analyze the determinants of self-medication behavior and willingness of Chinese residents. Methods: A total of 4 400 households from Beijing, Xi'an, Chengdu, and Kunming were chosen by multistage, stratified, and cluster sampling for a health survey. From a total 8 826 residents we selected 8 585 who aged over 15 years old and who gave a clear answer to whether had self-medication behavior and whether were willing to have self-medication. Logistic regression was used to analyze the relationship between self-medication behavior and self-medication willingness, and between residents' predisposing characteristics, enabling resources and need following Andersen health utilization model. Results: When other variables were controlled, the incidences of self-medication of residents at 25-44 years, 25-44 years, 45-64 years, and 65 years above were 1.52 folds (P = 0.000), 1.59 folds (P = 0.000), and 1.53 folds (P = 0.001) of the control group (15-24 years old). The incidence of self-medication of urban residents was significantly lower than that of the rural residents (P = 0.000). The incidence of self-medication behavior in residents with a common disorder in the last 6 months was 10.85 folds of those without common disease. The self-medication willingness of residents with chronic diseases was 1.22 folds (P = 0.035) of those without chronic diseases. The self-medication willingness of residents having a common disease in the last 6 months was 2.12 fold (P = 0.000) of those without common disorder. Conclusion: Age, urban-rural difference, knowledge of self-medication, and health status have different degrees of influence on the self-medication behavior and self-medication willingness of residents, while family income and health insurance have little influence.

Gastric cancer is one of the most common malignant tumors in China, with a high incidence and poor prognosis. It is still the focus of current work to improve the diagnosis rate and investigate the new treatment for gastric cancer. In recent years, intelligent medicine represented by artificial intelligence has been gradually applied in gastric cancer diagnosis and treatment, especially in gastroscopy examination, imaging examination, pathological diagnosis and so on. In terms of minimally invasive surgery for gastric cancer, the emergence of new technologies such as 4K and 3D have further improved the accuracy of surgery. At the same time, laparoscopic fluorescence imaging technology represented by indocyanine green imaging is gradually becoming a new direction for the development of minimally invasive surgery. Combined with the literature at home and abroad, the authors expound the application of minimally invasive intelligent technology in the process of diagnosis and treatment of gastric cancer to fully understand the opportunities and challenges faced by gastric cancer intelligent diagnosis and treatment, break the boundaries of disciplines, and carry out a wide range of 'medical-engineering integration′ research. This will finally benefit patients with gastric cancer.

Objective To investigate the effects of perfluorochemical ( PFC) on in vitro long-term hypothermic heart preservation in rats. Methods Thirty Wistar rats were randomly divided into control group ( Celsior solution), Celsior + O_2 group (Celsior solution plus oxygen) and PFC/Celsior + O_2 group (two-layer method plus oxygen) , with 10 rats in each group. Langendorff model of isolated rat heart was prepared. The isolated heart was preserved at 4 ℃ for 8 h, and hemodynamic parameters, coronary effluent flow, and leakage of creatine kinase ( CK), lactate dehydrogenase ( LDH) and aspartate transaminase (AST) were detected after reperfusion. Besides, the myocardial ultrastructure was also observed. Results Compared with control group and Celsior + O_2 group, the left ventricular developed pressure and ± dp/dt in PFC/ Celsior + O_2 group significantly increased ( P < 0.01), while LDH, CK and AST leakage significantly decreased ( P < 0.01). However, there was no significant difference in the above paramters between Celsior + O_2 group and control group (P>0.05). Compared with control group and Celsior + O_2 group, the impairment of myocardial ultrastructure in PFC/ Celsior + O_2 group after hypothermic preservation was alleviated. Conclusion PFC as a supplementation to oxygen in heart preservation solution could enhance myocardial protection during in vitro long-term hypothermic heart preservation in rats via the improvement of energy metabolism.