1.Research advancement of humidification in patients with artificial airway during mechanical ventilation
Chinese Journal of Practical Nursing 2017;33(z1):84-87
In order to improve the quality of humidification in patients with artificial airway during the mechanical ventilation, ensure the quality of artificial airway nursing. From the way of humidification , the choice of humidification liquid, the volume of humidification liquid control, the temperature of humidification liquid and the evaluation of humidification effect to research the present situation of humidification of artificial airway during mechanical ventilation. Put forward best way of patients with artificial airway nursing during the mechanical ventilation is wetting by temperature humidity of the Breathing machine′s and at the same time to strengthen the monitoring of the airway of humidification effect.
2.Differentiation of mesenchymal stem cell into epithelium and application
International Journal of Pediatrics 2011;38(6):616-619
Mesenchymal stem cells (MSC) is able to differentiate into a variety of epithelial cells,such as alveolar epithelium,tubular epithelium and corneal epithelial cells in vivo and in vitro under the given condition.After transfusion,MSC can effectively repair the injured lung epithelial cells,and relieve inflammation.MSC can also repair the renal tubular cell damage by reducing renal interstitial infiltration of inflammatory cells,promoting regeneration of renal tubular epithelial cells and inhibiting of apoptosis; Biological cornea can be available constructed by using MSC,which can availably repair the injured ocular surface.In the other hands,MSC can express keratinocyte growth factors and other cytokines,promoting limbal stem cell proliferation.In addition,MSC can also differentiate into pancreatic ductal epithelial cells,endometrial epithelial cells,spermatogenic epithelium and human retinal pigment epithelium.MSC has great application potential in epithelial diseases,and provide a good and effective choice in treatment of some epithelial diseases.
3.Comparative study of the application value of totally laparoscopic distal gastrectomy and laparoscopy-assisted distal gastrectomy for gastric carcinoma
International Journal of Surgery 2015;42(7):484-488
Laparoscope has been widely recognized as a treatment of distal gastric cancer.Totally laparoscopic distal gastrectomy and laparoscopy-assisted distal gastrectomy are two main types of laparoscopic distal gastrectomy.Apart from a smaller incision,some other advantages which totally laparoscopic distal gastrectomy has is the key point for further study.The thesis aims to compare totally laparoscopic distal gastrectomy to laparoscopy-assisted distal gastrectomy,and to analyze the characteristic in the aspect of operation time,the degree of pain,postoperative complications and the time of gastrointestinal recovery via collecting a great quantity of literatures.
4.Primary hypophysitis
Li ZANG ; Yi-Ming MU ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Primary hypophysitis includes 3 subtypes:lymphocytic,granulomatous and xanthomatous hypophysitis. The primary hypophysitis has a low morbidity and the final diagnosis is mostly determined based on the pathological findings after operation.Now it is considered as an organ specific autoimmune disease.Its clinical manifestations and imaging features are similar to hypophyseal tumor,but the endocrine and pathologic features are different.Glucocortieoid pulse therapy and surgery are the two methods of treatment.
5.Structuralization of Hospital Nursing Records of Community Child Health Care in Liaoning Province and its cross-mapping with International Classification of Nursing Practice
Shuang ZANG ; Xiaohan LI ; Yuli ZANG ; Jian WANG
Chinese Journal of Practical Nursing 2014;30(3):1-4
Objective To analyze the language structure of nursing records and the application of defined vocabulary in nursing records of community child care,and to explore the possibility of structuralization and standardization of community nursing records by researchers' consulting International Classification of Nursing Practice (ICNP(R)2.0).Methods Convenient sampling method was used.A total of 432 handbooks about child health care were extracted from three community health service centers in Liaoning province.By analyzing the nursing records,the vocabulary was selected,and a coding book was built.Then researchers compared them with ICNP(R)2.0 Chinese version.Results Totally 567 terms were extracted.All phrases in nursing records about community child health care can be classified into three categories and seven shafts,which were nursing phenomenon,nursing action and nursing outcome.Our community nursing records about child health care could not fully meet shaft level of nursing record writing requirements from the International Council of nurses.Only 146 records were labeled as perfect fit with ICNP(R)2.0 terminology,223 records as not fit at all.The difference of different levels of suitability distribution about nursing phenomenon,nursing action and nursing outcome had statistically significant difference.Words of able to adapt and can't adapt about nursing action had larger proportion.Words that can't adapt about nursing phenomenon and nursing outcome had larger proportion.Conclusions There is still certain gap between domestic nursing records and ICNP(R)2.0 terminology.The list of terms from comparison nursing records about community child health care with ICNP(R)2.0 can lay the foundation for nursing language system structure and perfect community nursing record language in our country.
6.Effect of organic solvents on human lungcancer cells and effectiveness of curcumol
Fangrui DONG ; Shilei ZANG ; Lili LI ; Jiquan GUO ; Linquan ZANG
Chinese Pharmacological Bulletin 2017;33(4):506-511
Aim To explore the concentration range of organic solvent which can both effectively increase solubility of the difficult soluble medicine monomer, and have low toxicity to cells, and to clarify the influence of different concentration of ethanol on curcumol efficacy.Methods Different DMSO and ethanol concentrations were diluted in culture medium and incubated with cells A549, NCI-H460, NCI-H1299, NCI-1650, LTEP-a2 and SPC-A1 for 12 h, 24 h, or 48 h, cell viability was tested by a colorimetric assay with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT), and different concentrations of ethanol with/without different concentrations of curcumol were also prepared with culture medium, then incubate with A549 and NCI-H460 cells for 24 h, and cell viability was tested by MTT as well.Results Within 48 h the solution with 0.008(V/V) DMSO or less had no significant effect on the cell A549 compared with control group, for NCI-H1650 the concentration was 0.004(V/V) or less, for NCI-H460 the result turned to be 0.002(V/V) or less, and the solution with DMSO below 0.001(V/V) had significant effect on the three other cells, NCI-H1299, LTEP-a2 and SPC-A1.While within 48 h, the liquor with 0.004(V/V) ethanol or less did not exhibit significant cytotoxic effect on the cell A549, for NCI-H460 and NCI-H1650 the result of ethanol concentration became 0.002(V/V) or less, for NCI-H1299 the data was 0.001(V/V) or less, and the liquor with ethanol below 0.001(V/V) showed significant cytotoxic effect on LTEP-a2and SPC-A1.When the proportion of ethanol in solution was below 0.01, it has no cytotoxic on cell A549 and NCI-H460, and the curcumol solution prepared with this kind of ethanol solution only represented the efficacy of curcumol on the cells.Since the solution with 0.01(V/V) ethanol dissolved the curcumol better, the cell viability changed from about 100% to about 35%.Conclusions Different organic solvent expressed different toxicity to the same cell, and the sensitivity of different cells to one organic solvent is dissimilar.and DMSO could be the optimum solvent for A549 and NCI-H1650, while the optimum solvent of NCI-H1299 is ethanol, for NCI-H460 it could be both and DMSO and ethanol, and DMSO and ethanol is not suitable for LETP-a2, SPC-A1 to be solvent.
7.Impact of pharmacogenetics on the drug therapy of epilepsy.
Li-li ZANG ; Li WANG ; Ye WU
Chinese Journal of Pediatrics 2011;49(8):599-602
8.Effects of perfluorochemical on in vitro long-term hypothermic heart preservation in rats
Jie XIANG ; Zhoubin LI ; Wangfu ZANG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(11):1331-1335
Objective To investigate the effects of perfluorochemical ( PFC) on in vitro long-term hypothermic heart preservation in rats. Methods Thirty Wistar rats were randomly divided into control group ( Celsior solution), Celsior + O_2 group (Celsior solution plus oxygen) and PFC/Celsior + O_2 group (two-layer method plus oxygen) , with 10 rats in each group. Langendorff model of isolated rat heart was prepared. The isolated heart was preserved at 4 ℃ for 8 h, and hemodynamic parameters, coronary effluent flow, and leakage of creatine kinase ( CK), lactate dehydrogenase ( LDH) and aspartate transaminase (AST) were detected after reperfusion. Besides, the myocardial ultrastructure was also observed. Results Compared with control group and Celsior + O_2 group, the left ventricular developed pressure and ± dp/dt in PFC/ Celsior + O_2 group significantly increased ( P < 0.01), while LDH, CK and AST leakage significantly decreased ( P < 0.01). However, there was no significant difference in the above paramters between Celsior + O_2 group and control group (P>0.05). Compared with control group and Celsior + O_2 group, the impairment of myocardial ultrastructure in PFC/ Celsior + O_2 group after hypothermic preservation was alleviated. Conclusion PFC as a supplementation to oxygen in heart preservation solution could enhance myocardial protection during in vitro long-term hypothermic heart preservation in rats via the improvement of energy metabolism.
9.Comparative Study on the Level of Resistin,Adiponectin between Blood Stasis Syndrome and Non-Blood Stasis Syndrome in Diabetic and Primary Hypertension Patients
Guihai CHEN ; Li WU ; Zhiming ZANG
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(05):-
Objective To investigate the correlation between resistin,adiponectin and the blood stasis syndrome in diabetic and primary hypertension patients.Method The primary hypertension and diabetes patients were drawn vein blood to make plasma and serum.Enzyme linked immunosorbent assay(ELISA) method was used in checking serum adiponectin and resistin.Radioimmuoassay method was applied in checking serum insulin,leptin,ET and tumor necrosis factor(TNF).The fasting blood sugar,total cholesterol(TC),triamid-glycerin(TG),high dense lipoprotein-cholesterol(HDL) and low dense lipoprotein-cholesterol(LDL) were detected by the chemi-enzymic method.The relation between the indexes and blood stasis syndrome was analyzed by SAS9.1 for windows.Results The detectable rate of the blood stasis syndrome in the diabetic patients was 0.423,while 0.246 in the primary hypertension patients.The difference was significant in statistics.Incidence of blood stasis syndrome increased with the course of illness.Compared with the non-blood stasis syndrome group,the content of leptin,ET and TNF in the blood stasis syndrome group significantly raised,but the content of adiponectin and insulin diminished.The content of resistin did not have obviously change.Compared with the primary hypertension patients,the level of leptin,adiponectin,resistin,insulin in the diabetic patients raised,but there wasn't significant difference in the level of ET and TNF.Conclusions There is negative correlation between adiponectin and the blood stasis syndrome.The link between obesity and the blood stasis syndrome may be through fat factor regulating vasoactive factor.The blood stasis syndrome caused by obesity maybe have nothing to do with the consistency of resistin.
10.Advances in Study on Functional Magnetic Resonance Imaging in Irritable Bowel Syndrome Comorbid with Anxiety and Depression
Tian LI ; Xiucai FANG ; Yufeng ZANG
Chinese Journal of Gastroenterology 2015;(6):369-372
Anxiety and depression are common conditions among patients with irritable bowel syndrome(IBS)which may induce or aggravate intestinal symptoms. Psychological factors are considered to be involved in the pathogenesis of IBS via both central and peripheral pathways,and functional magnetic resonance imaging(fMRI)revealed that several brain areas,such as anterior cingulate cortex and prefrontal cortex,are associated with IBS,anxiety and depression. This article reviewed the use of fMRI for studying the correlation of IBS brain activity with anxiety and depression and its role in the central pathogenesis of IBS.