This paper discussed multiple teaching methods used in classroom instruction which are used for Medicianl Chemistry Education of Preclinical Medicine Graduate Students,which were proved to be effective.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Indoles ; therapeutic use ; Liver Neoplasms ; drug therapy ; Male ; Middle Aged ; Pyrroles ; therapeutic use

Objective To analyzed the use of oral hypoglycemic drugs in our hospital for reference of clinical rational use of this kind of drugs. Methods The sorting methods of defined daily dose (DDDs) and the frequency of oral hypoglycenic drugs were used for analysis. Results The list of leading DDDs was glucosidase inhibitor, which accounted for 31.13 % of the total, followed by biguanides which accounted for 21.49%. Conclusions The glueosidase inhibitor, a new type oral hypoglycemic drugs used for lowering postprandial blood sugar level showed remarkable efficacy and safety, so it is currently the first choice for patients with diabetes.

Objective To observe the effect of blood-cooling and blood-stasis-removing Decoction on anti-double-strain DNA ( anti-dsDNA) antibody, anti-nucleosome antibody ( AnuA) and serum levels of interleukin ( IL) -6, 17, 21 in spontaneous systemic lupus erythematosus ( SLE) MRL/lpr experimental rats. Methods The experimental rats were divided into blank control group, model group, and high- and low- dose Chinese medicine groups ( 25.2, 12.6 g/kg respectively) , the treatment lasting 8 weeks. At the end of the experiment, the blood taken from the orbital veins was separated for obtaining serum, and then the serum anti-dsDNA antibody, AnuA, IL-6,17,21 levels were tested by enzyme-linked immunosorbent assay ( ELISA). Results The serum autoantibody and IL-6, 17, 21 levels of rats in the model group were increased significantly as compared with the blank control group ( P<0.01). The serum antibody and cytokine levels of Chinese medicine groups were reduced as compared with the model group, the difference between high-dose Chinese medicine group and model group being significant ( P<0.01). Conclusion Blood-cooling and blood-stasis-removing Decoction has certain effect on reducing the serum levels of anti-dsDNA antibody, AnuA, and IL-6,17,21, which may coniribute to one of its therapeutic mechanisms for SLE.

Objective:To study the effect of calcium sodium phosphosilicate(CSP)on the enamel remineralization of primary teeth. Methods:30 extracted human healthy primary molars were collected.Each tooth was cut bucco-lingually and mesial-distally into 4 sections.The sections were assigned randomly into 4 groups(n =30).Demineralization and remineralization cyclic model was estab-lished by etching with 35% phosphate acid for 2 min and then treated by unexposure of the sample to nothing(control,group A),so-dium monofluorophosphat(MFP,group B),CSP(group C)and MFP +CSP(group D).The cyclic was repeated twice daily for 30 d. The enamel surface morphology was observed by SEM and the surface microhardness(SMH)was measured.Results:On the tenth day,squamous morphology was observed on the enamel surface of group A and that of group D appeared less demineralization.The SMH value of group D was significant higher than that of group A(P <0.05).On the thirtieth day,obvious demineralization was ob-served in group A.Group B,C and D appeared surface remineralization.The SMH value of group B,C and D was significant higher than that of group A(P <0.05).Highest SMH value was achieved in group D.Conclusion:CSP combined with fluoride is more ef-fective in the enamel remineralization of primary teeth than the single application of them.

Objective To.investigate the accuracy and reliability of monocyte count of three methods.Methods The specimens classified by blood cell analyzer WBC monocytes ＜ 15％,15％-20％,＞ 20％ were taken randomly,draw 20 μ l after mixing,put in monocyte direct count dilution and count,and underwent routine smear,stained by Swiss-Gibbs and manual classified by microscope,and then compared the results of the three methods.Collected one copy each of the high,medium and low proportion of monocytes specimens,and counted 30 times by the three counting method.Results Blood cell analyzer in WBC monocytes 15％-20％,＞ 20％ had poor correlation with the results of the other two methods.Repeatability comparison of three methods,medium and low sample were better with method 1 and 2,high value sample was good with method 3.Conclusion The specimens of monocytes ≥ 15％,especially scatter plot is abnormal,must be confirmed by microscopic examination.

Objective:To evaluate curative impact of booster immunization with paternal lymphocytes on recurrent spontaneous abortors(RSA)with less reaction of primary patermal lymphocyte immunization.Methods:RSA patients with insufficient materno-fetal immuno-recognition were selected by flow cytometry of blocking antibody analysis and immunized with either induced paternal lymphocytes pretreated by IFN-? in vitro to those of anti-CD3-BE and anti-CD4-BE Ab lower than 0% or direct intradermal vaccination with their paternal lymphocytes without IFN-? pretreatment to those of anti-CD3-BE Ab beyond 0%.Reassessment of blocking antibodies was performed at the end of the second immnunization course.Results:Levels of blocking antibodies were significantly raised after the secondary booster immunization in RSA with insufficient materno-fetal immnuno-recognition whose blocking antibodies continuously decreased after being treated by the primary paternal lymphocyte immunization.No improvement of parameters was observed except the blocking effect in patients receiving secondary direct intradermal vaccination treatment.Conclusion:It is necessary for the RSA with insufficient materno-fetal immuno-recognition to experience secondary booster immunization preferably with paternal lymphocytes pretreated by IFN-? in vitro.

Angiocardiography ; Anti-Infective Agents ; therapeutic use ; Aorta ; abnormalities ; surgery ; Bronchopneumonia ; diagnosis ; drug therapy ; Cardiac Surgical Procedures ; methods ; Ductus Arteriosus, Patent ; diagnosis ; surgery ; Female ; Heart Defects, Congenital ; diagnosis ; surgery ; Humans ; Infant ; Pulmonary Artery ; abnormalities ; surgery ; Tomography, Spiral Computed

Objective To examine the number of fetal nucleated red blood cell (NRBC) in maternal blood and placenta tissue in fetal growth restriction(FGR) pregnancies. Methods 20 women of 28-36 weeks' gestation at age of 21~30(including 9 FGR pregnancies)were chosen. Fetal cells were isolated from maternal blood with discontinuous density gradient centrifugation. The isolated cells were made smear and counted under the microscope; After delivery, the placenta tissue were made into sections and also counted under the microscope; To determine the origin of the NRBC , the single NRBC was analysed by primer extension preamplification (PEP) and polymerase chain reaction (PCR). Results The number of NRBC in 9 FGR pregnancy women's peripheral blood ranged from 12/7 ml~40/7 ml,(average 22.6/7 ml). The number of NRBC in the control pregnancies ranged from 0/7 ml~10/7 ml, (average 5.4/7 ml). Significant difference was shown between the two groups; The number of NRBC in 9 FGR pregnancy women's placenta tissue was significantly higher than the median value in the control pregnancies (2.8/20HP compared with 0.6/20HP, P

Objective To study the effec of nursing intervention on the quality of life in convalescent schizophrenia patients. Method 80 patients with schizophrenia were divided into observed group (n=40) and control group (n=40).The patients in observe group received nursing intervention.Patients were evaluated by Positive and Negative Symptoms Scale (PANSS) and General Quality of Life Inventory (GQOLI) at the time of beginning the trial and after half of a year of the intervention respectively. Results After the nursing intervention,the PANSS scores in observe group are significant lower than those of control group (P