1.Effect of RGD-insulin on activities of bone resorption and the possible mechanism in human osteoclast-like cells in vitro
Chinese Journal of Geriatrics 1995;0(02):-
Objective In this study, we explored the mechanism of anti-bone resorption of RGD-insulin using osteoclast-like cells (OLCs) from giant cell tumor of bone as an in vitro model. Methods The function of bone resorption was observed and the staining for tartrate-resistant acid phosphatase (TRAPase) was identified. The carbonic anhydrase Ⅱ (CAⅡ) gene expression, apoptosis and the adhesion of OLC that treated by RGD-insulin and echistatin were determined by in situ hybridization, TUNEL staining and adhesion test, respectively. Results OLCs were TRAP positive and had bone resorptive function. After OLCs were treated with 10 -5, 10 -6, 10 -7mol/L RGD-insulin and echistatin respectively, the numbers of TRAP-positive multinucleated cells were (179?3), (238?11), (351?10) per well that treated with echistatin and (229?20), (222?21), (265?17) per well that treat with RGD-insulin.The expression of CAⅡ mRNA decreased, the number of OLCs apoptosis increased in a dose-dependent manner (P
2.Research on the relationship between miRNAs and peritoneal fibrosis
Tianjin Medical Journal 2016;44(10):1287-1290
In recent years, microRNAs (miRNAs) have been found to be one of the key factors of post transcriptional gene regulation, which are involved in occurrence and development of many diseases. Peritoneal dialysis (PD) has become an effective alternative treatment approaches for patients with end stage renal disease (ESRD). Peritoneal fibrosis is one of the most important factors leading patients to withdraw from long-term PD, hence restricts the application and development of PD. MicroRNAs are closely related to the development of peritoneal fibrosis. This article reviews the role of miRNAs in the pathogenesis and treatment of peritoneal fibrosis.
4.Clinical analysis of chronic cough caused by postnasal drip syndrome.
Yong-Dong YAN ; Li HUANG ; Wei JI
Chinese Journal of Contemporary Pediatrics 2008;10(1):79-80
Adolescent
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Child
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Child, Preschool
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Chronic Disease
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Cough
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etiology
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Female
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Humans
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Male
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Rhinitis
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complications
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diagnosis
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drug therapy
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Sinusitis
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complications
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diagnosis
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drug therapy
5.Ovarian steroid cell tumor: clinicopathologic study of 6 cases.
Chinese Journal of Pathology 2009;38(9):621-622
12E7 Antigen
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Adult
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Antigens, CD
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metabolism
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Calbindin 2
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Cell Adhesion Molecules
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metabolism
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Female
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Follow-Up Studies
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Humans
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Hysterectomy
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methods
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Inhibins
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metabolism
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Middle Aged
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Ovarian Neoplasms
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metabolism
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pathology
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surgery
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Ovariectomy
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methods
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Retrospective Studies
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S100 Calcium Binding Protein G
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metabolism
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Sex Cord-Gonadal Stromal Tumors
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metabolism
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pathology
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surgery
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Young Adult
6.Preventing the recurrence of condyloma acuminatum with different treatment courses of imiquimod cream
Dong-Yan HU ; Lei CHEN ; Ming LI ;
Chinese Journal of Dermatology 1994;0(05):-
Objective To evaluate the efficacy and safety of imiquimod 5% cream applied with different treatment courses for preventing the recurrence of condyloma acuminatum lesions.Methods A randomized,controlled clinical trial was conducted.Patients with condyloma acuminatum were divided into 3 groups (Group A,B and C).Group A was treated with CO2 laser only.Group B was given topical im- iquimod 5% cream three times a week over 4 weeks after laser treatment of visible warts.Group C was giv- en topical imiquimod 5% cream three times a week over 8 weeks after laser treatment of visible warts.All patients were followed up for 6 months.Results A total of 90 patients with anogenital warts were enrolled in,and finished the study.The recurrence rates of the Group A,B,and C were 64.29%,50%,21.88%,re- spectively,in 6 months of follow-up.The recurrence rate was statistically lower in Group C than in Group A and B (P=0.0033),and in Group C than in Group B (P=0.0457).Adverse reactions occurred in 73.33% and 84.38% of patients in Group B and C,respectively,which included erythema,itching,pain, burning,erosion,ulceration and flu-like symptoms.There was no significant difference of the frequencies of adverse reactions between Group B and C (P>0.05).Conclusion The recurrence rate of anogenital warts declines significantly in patients treated with imiquimod 5% cream for 8 weeks,but not in the 4 weeks group,after laser therapy.The frequencies of adverse reactions are not significantly different in both groups.
7.Comparison analysis between different analysis systems for the detection of electrolytes
Yan WU ; Quan LI ; Shanshan DONG
International Journal of Laboratory Medicine 2015;(8):1108-1109,1112
Objective To investigate the comparability of K + ,Na+ and Cl- measurement between Cobas8000 analyzer and AU640 analyzer .Methods According to the EP9‐A2 document of the National Committee for Clinical Laboratory Standards ,the precision of AU640 analyzer for the detection of K + ,Na+ and Cl- was evaluated ,and the correlation between the detected results of the two analyzers were also analyzed .Results AU640 analyzer were with high precision for the detection of K + ,Na+ and Cl- ,in‐tra‐assay and inter‐assay coefficient of variation of which were both less than 2% .The correlation coefficients of the two analyzers were higher than 0 .975 .Conclusion Cobas8000 analyzer and AU640 analyzer might be with fine comparability for the detection of K+ 、Na+ and Cl- ,both of which could provide accurate detected results .
8.Experience of diagnosis of 56 cerebral aneurysms cases by 16-slice spiral CTA
China Medical Equipment 2015;(7):93-95,96
Objective:To evaluate the clinical value of 16-slice spiral CTA and methods of diagnosis of cerebral aneurysms.Methods: Fifty six cases of cerebral aneurysms with CTA image data were retrospectively analyzed from July 2012 to July 2014. Omnipaque (350 mgI/100 ml) as contrast agent was used in all cases with injection speed of 4.0 mL/s. The methods of VR, SSD, MIP, MPR, CPR and VE were involved in CTA images reconstruction and processing were implemented with workstation ADW4.2. The diagnosis value of cerebral aneurysms with different methods of vessel reconstruction was analyzed.Results: The location and the size of intracranial aneurysms could be clearly showed with 16-slice spiral CTA. The diagnosis time of methods VR and MIP was shortest and the mean time was 4.3 minutes and 5.1 minutes respectively. There was significant difference between the image and actual size in berry aneurysms with SSD method.Conclusion: The synthetic application of multimethod can improve the diagnostic value of 16-slice spiral CTA in cerebral aneurysms.
9.In vitro pre-vascularized tissue-engineered bone
Yan LI ; Jianshe ZHANG ; Xiuhua DONG
Chinese Journal of Tissue Engineering Research 2013;(33):6013-6020
BACKGROUND:Tissue-engineered bone, as an emerging method, is used to repair bone defects, but it is difficult to be used widely because of no nutrient metabolism of implants. Pre-vascularized tissue-engineered bone research is performed for this limitation. Forward-looking and procedural vascular construction prior to artificial grafting is perspective to supply the nutrient metabolism of implants.
OBJECTIVE:To analyze the research results and developmental tendency of pre-vascularized bone engineering in vitro using a multilevel analysis.
METHOD:Literature search was performed in CNKI database for Chinese literatures and PubMed database for English literatures from 2000 to 2012. The key words included“tissue-engineered bone, vascularization, implant, osteoblasts, endothelial cel s, co-culture”in Chinese, and“bone engineering, endothelial cel s, osteoblast, implant, cel s co-culture”in English. A review addressing pre-vascularized tissue-engineered bone was completed based on retrieved literature classified according to bone physiological studies, in vitro experimental research and material research.
RESULTS AND CONCLUSION:Total y 60 articles were included in result analysis. Studied have documented that microvascular regeneration in normal bone tissue has an important role in osteogenesis regulation. Current bone tissue engineering research is to simulate this physiological process of vascular regeneration in the human body, and lots of in vitro studies are designed to complete this angiogenic process in bone tissue engineering. It is confirmed that in vitro pre-vascular work is used for bone tissue engineering, especial y for in vivo survival and ossification of tissue-engineered bone, pointing out the developmental direction for the clinical application of tissue-engineered bone.
10.Power transformer fault diagnosis model based on rough set theory with fuzzy representation
Minghua LI ; Ming DONG ; Zhang YAN
Journal of Pharmaceutical Analysis 2007;19(1):9-13,55
Objective Due to the incompleteness and complexity of fault diagnosis for power transformers, a comprehensive rough-fuzzy scheme for solving fault diagnosis problems is presented. Fuzzy set theory is used both for representation of incipient faults' indications and producing a fuzzy granulation of the feature space. Rough set theory is used to obtain dependency rules that model indicative regions in the granulated feature space. The fuzzy membership functions corresponding to the indicative regions, modelled by rules, are stored as cases. Results Diagnostic conclusions are made using a similarity measure based on these membership functions. Each case involves only a reduced number of relevant features making this scheme suitable for fault diagnosis. Conclusion Superiority of this method in terms of classification accuracy and case generation is demonstrated.