Objective To investigate human esophageal cancer Eca109 cells apoptosis induced by resveratrol.Methods Eca109 cells were treated with difierent concentrations of resveratrol and the change of morphology was observed by inverted microscope and transmission electron microscope in different time;The cell growth inhibitory rate of resveratrol on the proliferation of Eca109 cells were evaluated in vitro by MTT assay.Flow cytometry (FCM)analysis was used to determine the apoptosis of Eca-109 cells.Results Resveratrol was able to inhibit the growth of Eca109 cells,and the suppressive effect was increased with treating concentration and treating time.Resveratrol inhibited the growth of esophageal cancer Eca109 cells in a dose-and time-dependent manner and induced the apoptosis in esophageal cancer.Conclusion Resveratrol could inhibit the proliferation of Eca-109 cells and block the cell cycle at G0/G1.And in the end resveratrol could induce the apoptosis of Eca109 cells.

Objective To investigate the possible mechanism of apoptosis effect induced by resveratrol in human acute T lymphoblast leukemia Molt-4 cells. Methods MTT method was used to analyze the inhibition rate. The cell cycle and apoptosis percentage of Molt-4 cells treated with resveratrol were detected by flow cytometry. The expression of WAVE1 mRNA was assessed by semi-quantitative RT-PCR. Results After treated with 12.5, 25.0, 50.0, 100.0, 200.0μmol/L resveratrol for 24, 48 and 72 hours, the inhibitory effects were at 29.32 %, 36.11 %, 53.92 %, 62.50 %, and 74.98 %, respectively. Resveratrol was able to inhibit the proliferation of Molt-4 cells in a time-dependent and dose-dependent manner (F =33.614, P <0.05).Compared with control group, after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the cell number in S phase of Molt-4 cells was 68.6 % and 78.1 %, respectively, and the cell cycle was arrested at S phase (F = 19.453, P < 0.01) after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the ratio of WAVE1/GAPDH was 0.356±0.03, 0.382±0.05. Compared with control group 0.586±0.06, the ratio of WAVE1/GAPDH was decreased (F =8.950, P <0.01). Conclusion Resveratrol could induce the cells to undergo apoptosis, which was probably related to downregulation the expression of WAVE1.

Nerve growth factor (NGF) has been the focus in area of angiogenesis.In differential,survival,development of nerves,NGF plays a crucial role.In addition,NGF activates migration,differential of endothelial cells and vascular smooth muscle cells,and stimulate the activation of the endothelial progenitor cells in angiogenesis.Crosstalk between vascular endothelial growth factor and NGF is also critical in the development of vessels.The function of NGF in angiogenesis blazes a trail in therapy of ischemia diseases.

Objective To sum up the experiences of tongue repair after radical operation of tongue carcinoma with free forearm flap in the last two years. Methods The clinical data of 26 patients underwent forearm free flap transfer for tongue repair from February 2002 to February 2004 were retrospectively analysed . Results Among the 26 grafted forearm flaps, 25 survived. Only one flap occurred arterial thrombosis and necrosis after the second operation.All the patients were followed up for 6 months to 2 years. Reconstruction tongues had suitable volume and shape as well as perfect movement ability. Conclusion Free forearm flap was suitable to repair the tongue defect after radical operation of tongue caicinoma. The rate of operation success was high, and reconstructed tongues could basically meet patient's normal life.

The molding of humanistic spirit in medical colleges and universities has a direct bearing on whether or not the cultivated medical students can establish fundamentally the mental idea of humanistic solicitude and orientation of value.This article expounds the importance and urgency of strengthening the molding of humanistic spirit in medical colleges and universities,and analyzes the current situations of construction of humanistic spirit in colleges and universities of our country at present,and investigates the ways and countermeasures of strengthening the molding of humanistic spirit in medical colleges and universities.

Objective To analyze the variation of peripheral blood Th17 cells and Foxp3+CD4+CD25+regulatory T (Treg)cells in patients with ankylosing spondylitis (AS),and to interpret the role of the ratio imbalance of peripheral blood Th17/Treg cells in the pathogenesis of AS.Methods The percentage of peripheral blood Th17 cells and Foxp3 +CD4+CD25+ Treg cells in AS patients and healthy subjects were determined by flow cytometry (FCM).The concentrations of IL-2,IL-10,TGF-β,IL-6,IL-17 and IL-23 in the serum were measured by enzyme linked immunosorbent assay.The correlation between these cytokines and traditional parameters was analyzed.T test,ANOVA,SNK-q and Speannan's analysis were used for statistical analysis.Results Compared with healthy controls,the percentage of Th17 cells in AS patients increased significantly (P＜0.05); Compared with the inactive AS patients,the percentage of Th17 cells in active AS patients increased significantly [(2.10±0.82)％,(2.89±1.77)％,P＜0.05)]; the percentage of Foxp3+CD4+CD25+ Treg cells in active AS patients was markedly lower than healthy controls (P＜0.05).Compared with healthy controls and inactive AS patients,the specific value of Th17/Treg in active AS patients increased significantly(P＜0.05).The concentrations of IL-17 and IL-23 was found significantly higher in patients with AS than that in healthy controls (t=2.203,P=0.032 and t=2.088,P=0.041).The concentrations of IL-17 and IL-23 was found significantly higher in patients with active AS than that in inactive AS (t=2.808,P=0.008 and t=2.242,P=0.020).The correlation analysis revealed that these cytokines and traditional parameters was not relevant except IL-17/BASDAI (r=0.32,P=0.044).Conclusion The increased ratio of Th17 cells and decreased ratio of Foxp3+CD4+CD25+ Treg cells may play a critical role in AS progression.Manipulating the balance of Th17/Treg would provide a new approach for the treatment of AS.

Objective To evaluate the technical success,adverse events in 30 days after endovascular repair for acute and non-acute Stanford B aortic dissection.Methods A search was performed to identify all Chinese literatures that compare the effect of TEVAR on acute and non-acute Stanford B dissection in WanFang and Chinese National Knowledge Infrastructure (CNKI) from 2009 to 2014.Literatures were extracted and x2 test conducted by SPSS 17.0 software,REVMAN 5.3 software was used to conduct meta-analysis and bias analysis by STATA software.Results 5 studies including 468 patients (acute group 243,non-acute group 225) were extracted.Acute Stanford B dissection have higher rate both in total complications(OR =3.10,95％ CI =[1.74,5.53],P =0.0001) and endoleaks (OR =2.07,95％ CI =[1.11,3.88],P =0.02) within 30 days than non-acute group,the technique success rate (P =0.3),the mortality rate within 30 days (P =0.27) as well as aortic related mortality rate (P =0.26)show no significant difference.Conclusion Acute Stanford B dissection have higher rate of total postoperative complications,endoleaks within 30 days than non-acute group,with similar surgical success rate,30 days mortality rate and aorta related mortality rate.

Objeetive To analyze the risk factors on anastomotic leakage after low anterior resection with the technique of total mesorectal excision(TME)for rectal carcinoma. Methods Our retrospective study included 498 patients of rectal cancer who underwent anterior resection with TME technique. Results 7.2%patients(36/498)developed clinical anastomotic leakage.Sex,distance of tumor from anal verge,preoperative radiotherapy were found related to the anastomotic leakage ( P＜0.05 or0.01),while age,diabetes,hypertensive disease,vesse]emboli,diversion stoma construction,anastomotic technique were not.Among the 37 patients with diversion stoma construction simultaneously,3 patients developed leakage who recovered conservatively.Among the other 33 leakages 10 were cured conservatively.The other 23 leakages necessitated a laparotomy and proximal colostomy. Conclusion Sex,distance of tumor from anal verge,preoperative radiotherapy were risk factors for anastomotic leakage after TME.

Objective To discuss the value of vertebral hemangiomas treated with the CT-guided pereutaneous vertebroplasty (PVP).Methods Forty-two patients with 49 vertebral lesions were guided by CT and injected with bone cement percutaneous into the vertebral.The effectiveness was valued by preoperative and postoperative visual analogue scale (VAS),locomotor activity scores,pain medication scores and comprehensive evaluation of long-term efficacy of WHO criteria,and undertook statistical analysis.Results The success rate in all patients was 100.0％(42/42),no serious complications.All the patients were followed up for 6 - 18 ( 12.50 ± 1.15 ) months,all lesions of the vertebral body imaging showed no tumor recurrence and vertebral collapse.The VAS scores decreased from preoperative (7.64 ± 1.29 ) scores to postoperative 1-3 days (4.82 ± 1.21 ) scores and to postoperative 3 -6 months (0.22 ±0.19) scores (P＜0.05).The locomotor activity scores decreased from preoperative (3.4 ± 0.9) scores to postoperative 1-3 days (2.1 ±0.6) scores and to postoperative 3-6 months (1.0 ±0.3) scores (P＜0.05).The pain medication scores decreased from preoperative ( 1.9 ± 0.6) scores to postoperative 1 - 3 days months ( 1.1 ± 0.2 ) scores and topostoperative 3-6 months (0.2 ± 0.1 ) scores (P ＜ 0.05 ).Comprehensive evaluation of long-term efficacy of WHO criteria:efficiency rate was 97.6％(41/42).Conclusions CT-guided percutaneous vertebroplasty for vertebral hemangioma can effectively relieve the pain caused by vertebral body to increase stability,improve motor function,which is a method with safe,minimally invasion,effect,and is superior than C-arm fluoroscopy.

The proteomics definition, investigation method and its a pplication in cancer research were simply introduced. Proteomic research is to r eveal the function of genes from an integrated, kinetic and quantitative view at the global protein level, which is an important component of post-genome proje ct. Cancer is a kind of complex disease involved by multi-genes. Proteomic rese arch will be helpful to discover the mechanism of cancer development, to find sp ecial malignant tumor markers and targets of drug treatment.