Medical social work is a new arising occupation that provides professional medical service for patients in hospitals and communities based on the development of new medical mode,playing critical role in the therapeutic rehabilitation of certain diseases.Tumor diseases,especially malignant tumors,are serious hazards to human life and health,thus require special medical treatment and therapeutic rehabilitation due to their specific characteristics.Therefore,intervention of medical social work is of irreplaceable significance in the therapeutic rehabilitation of tumor diseases,promoting the realization of rehabilitation,soothing physical pains,providing psychological support,lengthening life expansion,enhancing the living quality of tumor sufferers,and assisting them better to regress to normal social life.

Theauthor treated 148 cases of hiccup by finger-pressing Yifeng (TE 17). After one treatment, 97cases were cured; 34 cases were cured after 2-3 treatments, 6 cases were cured after over 4 treatments; and 11 cases obtained no effect. The total effective rate was 92.6%.

OBJECTIVE To establish a method of real-time PCR for detection of mecA gene in meticillin-resistant Staphylococcus aureus(MRSA).METHODS The experiment conditions including Mg2+ concentrations,primers concentrations and probe concentrations were optimized for real-time PCR in the light of factorial design principle.Totally 109 strains of S.aureus were collected and detected respectively in oxacillin disk diffusion method and real-time PCR and MRSA detection rate in the two methods were compared and analyzed.RESULTS Thirty seven MRSA isolates were detected by real-time PCR from the 109 S.aureus strains.The MRSA isolating rate by real-time PCR was conspicuously higher than oxacillin disk diffusion method,by which 27 MRSA isolates were detected.CONCLUSIONS The technology of real-time PCR to rapid identification of MRSA is superior to common PCR and it can make up the deficiency of detecting borderline-resistant strains in oxacillin disk diffusion method.

OBJECTIVE To establish and evaluate detection method of meticillin-resistant Staphylococcus aureus(MRSA) by fluorescence in situ hybridization(FISH) and flow cytometry.METHODS The experiment conditions including bacteria suspension concentrations,probe concentrations,and hybridization temperatures were optimized for FISH test in the light of orthogonal design principle.The fluorescence signals were detected by flow cytometer,and detection results by FISH and flow cytometry were compared with that of real-time PCR.RESULTS The optimal hybridization conditions of FISH detecting MRSA were 40?105 of bacteria suspension concentrations per microliter of hybridization buffer solution,and 4 ng of probe concentrations per microliter of hybridization buffer solution and 50 ℃ of hybridization temperatures.The sensitivity and specificity of detecting MRSA by FISH-FCM were 97.3% and 100.0%,respectively,and detection results between FISHFCM and real-time PCR had not significant deviation.CONCLUSIONS Fluorescence in situ hybridization and flow cytometry,which detect directly mecA gene in MRSA, are a fast and accurate methods for MRSA detection and can apply in clinical laboratory.

Objective To investigate the expression of insulin receptor substrate-2(IRS-2)in the liver of nonalcoholic fatty liver(NAFL)mice.Methods Ten female C57BL6J mice fed with normal diet were served as controls,while fifteen fed with high fat and sugar diet for either 8 weeks(group-A)or 16 weeks(group B)were as NAFL mice models.Abdominal fat mass,total cholesterol(TC),triglycerides(TG),high density lipoprotein cholesterol(HDL-C),fasting blood glucose(FBG)insulin(INS)and liver function,liver weight were measured.And the expression of IRS-2 and the content of lipid in the liver were also detected.Results The abdominal fat mass serum TC,and FBG were significantly higher in high fat and sugar diet fed mice than that in controls(P

Objective To optimize the extraction process by determing the volatile oil yield of steam distillation and supercritical CO2 extraction from flowers of Elaeagnus angustifolia L..Methods Orthogonal test was carried out to compare two extraction effects between steam distillation and supercritical CO2 extraction by detecting the extraction ratio from flowers of Elaeagnus angustifolia L..Results Steam distillation time on volatile oil extraction has the greatest impaction,and the best condition of supercritical CO2 extraction was extraction pressure of 20 MPa,extraction temperature of 40 ℃,and flux of CO2 is 10 L/h.Conclusion Compared with steam distillation,supercritical CO2 extraction spends less time,has higher efficiency and less harm to the materiel after extracting.It adapts to batch process and is convenient for subsequent extraction technology.

OBJECTIVE:To investigate clinical efficacy and safety of conjugeted estrogen tablets combined with Salmon calci-tonin acetate injection and Alendronate sodium tablets in the treatment of osteoporosis in postmenopausal. METHODS:148 post-menopausal women with osteoporosis were randomly divided into observation group(78 cases)and control group(70 cases). Con-trol group was given Salmon calcitonin acetate injection hypodermically or intramuscularly,100 IU each time,once a day in first week,every two days in second week,every three days in third week,21 times of consecutive injection as a treatment course;Alendronate sodium tablet,70 mg each time,once a week,3 months as a treatment course. Based on control group,observation group was additionally given conjugeted estrogen tablets,0.625 mg each time,qd,for consecutive 3 weeks,drug withdrawal for one week,and then continued to taking drug,for 3 months in total. Clinical efficacy of 2 groups were observed as well as BGP, bone metabolism index as human collagen type Ⅰ N-terminal peptide (NTX) and ALP,bone density and ADR before and after treatment. RESULTS:After treatment,total effective rate of observation group was 94.87%,which was significantly higher than 78.57% in control group,with statistical significance(P<0.05);BGP,lumbar spine and thigh-bone density of 2 groups were all improved significantly,and the improvement of observation group was more significant than that of control group,with statistical significance (P<0.05). NTX and ALP of 2 groups were decreased significantly,and the decrease of observation group was more significant than that of control group,with statistical significance (P<0.05). The incidence of ADR was 12.82% in observation group and 8.57% in control group,without statistical significance (P>0.05);various ADR disappeared after drug withdrawal. CONCLUSIONS:Conjugeted estrogen tablets combined with Salmon calcitonin acetate injection and Alendronate sodium tablets can effectively relieve bone pain,regulate bone metabolism,increase bone density and induce slight ADR in postmenopausal with osteoporosis.

Objective To investigate the characteristic of theta oscillation in patients with frontal lobe epilepsy (FLE) by the analysis of multi-channel electroencephalographs (EEGs) during rest state and working memory(WM) maintenance. Methods The 19 FLE patients and 17 healthy subjects underwent EEG recording with 34-channel EEG machine during visual working memory task performance. The differences of behavioral results were analyzed between FLE group and controls. Short-time Fourier transform was used to calculate the power spectral density of different frequency bands in 34 channels. The theta power values during the retention period of working memory and rest state were compared between two groups. Results Compared with controls, reaction time of FLE increased significantly (P<0.01) and accuracy decreased (P<0.05). The power spectral density of theta band for FLE increased both in Fz and frontal region during rest state (P<0.01). Compared with controls, the power spectral density of theta oscillation decreased significantly in the frontal region for FLE during WM maintenance. Conclusion Our results suggest that working memory in patients with FLE was impaired. The absence of theta oscillation during the WM delayde period may provide a possible neural mechanism for the working memory dysfunction in FLE.

Objective:To explore the effects of myofibroblast in the pathogenesis of oral submucous fibrosis(OSF).Methods:The expressions of ?-SMA in the tissues of OSF and normal control were detected by immunohistochemistry. Fibroblasts were isolated from oral mucosa of OSF patients and healthy controls and were cultured under stimulation by arecoline.The expressions of ?-SMA in OSF fibroblasts and normal fibroblast were detected by immunocellchemistry and RT-PCR.Results:(1)No positive expression was found in normal tissues except in the blood vessel,but in OSF tissue,?-SMA positive expression was found in many spindle cells in the oral submucousal layer.(2)Few fibroblasts in vitro from normal controls were ?-SMA positive.(3)Most of the second passage of OSF fibroblasts were ?-SMA positive(85.80%?3.56%). (4)The expression of ?-SMA in fibroblasts could not be increased by the stimulation of arecoline.Conclusion:Myofibroblasts may play an important role in the pathogenesis of OSF,and the appearrence of myofibroblasts in OSF may not be directly induced by the stimulation of arecoline.

Objective:In order to elucidate the derivation and differentiation mechanisms for derivation of myofibroblasts in oral submucous fibrosis.Methods:Oral keratinocytes and fibroblasts were isolated and cultured,and a co-cultur system composed of the keratinocytes and fibroblasts was established in vitro.Expressions of ?-SMA protein and mRNA in fibroblasts were detected by immunohistochemistry and RT-PCR. The level of TGF-?1 in conditioned medium were evaluated by ELISA.Results:The expressions of ?-SMA mRNA and protein in fibroblasts co-cultured with keratinocytes preprocessed by arecoline was higher than that in fibroblasts co-cultured with keratinocytes and without preprocessed by arecoline. The level of TGF-?1 in conditioned medium of fibroblasts-keratinocytes preprocessed by arecoline co-cultures was higher than those of fibroblasts cultured alone.Conclusion:The derivation of myofibroblast from FB can be promoted by keratinocytes preprocessed by arecoline and TGF-?1 may play a role in the process.