OBJECTIVE: According to the holism in traditional Chinese medicine (TCM) theory, this manuscript explores the relativity of pathogenesis among hypertension, diabetes and hyperlipemia.DATA SOURCES: We applied computer to search articles in English on pathogenesis ofhypertension, diabetes and hyperlipemia in Medline between January 1985 and December 2004 by words "hypertension, diabetes, hyperlipemia, pathogenesis" .Besides, we also used computer to search articles in Chinese on pathogenesis of hypertension, diabetes and hyperlipemia in Chinese magazine full text database between January 1990 and December 2004 by words "hypertension, diabetes, hyperlipemia, pathogenesis".STUDY SELECTION: Primarily, articles on pathogenesis of hypertension,diabetes and hyperlipemia were selected in TCM and modern medicine respectively. Accepted standard were: ① Random control clinic test with methods of single blind, double blind or no blind; ② Nonrandom control test;③ Fore-and-aft control test; ④ Chapter in monograph. Eliminated standard:repeated research.DATA EXTRACTION: Totally 28 articles on pathogenesis of hypertension,diabetes and hyperlipemia were found in TCM and modern medicine, however, 12 articles were selected according to accepted standard, and 16 articles were eliminated due to repeated content.DATA SYSTHESIS: Totally 12 selected articles were analyzed on pathogeneses of hypertension, diabetes and hyperlipemia in TCM and modern medicine, and hinted internal affiliation of these three diseases from view of molecular biology.CONCLUSION: These three diseases can be regarded as a whole entity for study. High blood pressure, high blood sugar and high lipemia could be treated as different forms of the entity or in different developing courses of the whole. In a certain course it might present as one of the forms specifically. It might manifest high blood pressure mainly in clinic, or high blood sugar, or high lipemia,also several forms at the same time. It will be beneficial to take the holism and visceral theory in TCM for reference in research of modern medicine.

Intrauterine growth retardation (IUGR) can improve the danger of insulin resistance syndrome. It was reported that regulatory factors of βcells development and enzymes of regulating metabolism of glucose, fat, insulin signal transduction and effecter molecule were expressed at a low level in the IUGR. As a result, the number of β cells, proliferation, insulin secretion and insulin sensitivity was reduced, apoptosis was increased, and glucose intake in peripheral tissue declined. All these factors contribute to the insulin resistance syndrome.

Objective To study the influence of Orem self-care theory on postpartum quality of life and role change of parturients with natural labor. Methods 100 parturients with natural labor in our hospital from May 2010 to July 2011 were selected as research object,and they were randomly divided into the control group and the observation group with 50 cases in each group,the control group was taken care with routine nursing methods,the observation group was given with nursing according to Orem self-care theory.Then the SCL-90 score,situation of role change and satisfaction degree of two groups before nursing and on the second and fifth days after nursing were analyzed and compared. Results The SCL-90 score of the observation group after the nursing on second and fifth days was all lower than those of the control group,the better rate of role change was higher than that of the control group,patients'satisfaction degree was higher than that of the control group. Conclusions The Orem self-care theory plays an important role in improving the quality of life and role change of parturients with natural labor after delivery,and it can significantly improve satisfaction degree of parturients.

OBJECTIVE:To establish an HPLC method for determination of major component and related peptide in eptifibatide injection.METHODS:The determination was performed on AichromBond-AQ C18 column with column temperature at 25 ℃ and flow rate of 1.0 mL?min-1.The mobile phase consisted of acetonitrile-water(0.13% trifluoroacetic acid).The detection wavelength was set at 220 nm and injection volume was 20 ?L.RESULTS:The linear range was 75.2～1 203.6 ?g?mL-1(r=0.9999) with mean recovery of 99.73%～99.95% (RSD=0.26%).CONCLUSION:The method is simple and rapid and it is applicable for determination of major component and related peptide in eptifibatide injection.

Humans ; Tuberculosis, Hepatic

Calculi ; chemically induced ; Ceftriaxone ; adverse effects ; Child ; Humans ; Lithiasis ; chemically induced

Flow Cytometry ; instrumentation ; Indicators and Reagents ; Investigational New Drug Application

BACKGROUND: Dance-sport is a newly emerging sport item, integrating sport with music and dance. It not only develops physical diathesis and sport skills, but also actively affects psychological health of university students.OBJECTIVE: To analyze and observe the changes in psychological health, physical diathesis and musical cultivation after dance-sport training for university students.DESIGN: Cross-section investigation was designed, in which, the students in elective class of dance-sport were taken as objects.SETTING: Department of Physical Education of China University of Mining and Technology.PARTICIPANTS: Totally 116 students were collected from 2001' elective class of dance-sport in China University of Mining and Technology from September 2001 to January 2003. All of receptors selected dance-sport at the first time. Of 120 copies of Symptom Checklist 90 (SCL-90) were provided and 116 of those were returned, in which, 58 students were either male or female, aged averagely 19.7 years.METHODS: Questionnaire: SCL-90 was used to evaluate 9 main outcome measures, named psychological health, somatization, obsessive compulsive (o-c) symptoms, personal relations, depression, anxiety, hostility, phobism,paranoid ideation and metal disorder; in which, 5 grades were adopted,named 1, 2, 3, 4 and 5 grades, to represent no, mild, moderate, severe and extremely severe impacts successively. Test: according to stipulated music rate, cardiac rate and music were tested so as to observe the changes in physical diathesis and capacity of musical rhythm understanding. Simultaneously, literatures were looked up on education, psychology and physical education to provide theoretic evidence for this paper. The questionnaire was provided before and after the semester respectively and returned for music test. Test on cardiac rate was performed at the end of semester.MAIN OUTCOME MEASURES: Comparison among the results of two SCL-90 evaluations, positive number and total average result in receptors and national norm. Result of cardiac rate and music test in receptors.RESULTS: Totally 120 copies were provided, 116 of them were returned and the rate of return was 97%. ① Result of SCL-90: At the end of semester, the results of depression, hostility and positive number of 9 items in the checklist were lower remarkably than those before the semester (P ＜ 0.01-0.05); the results of somatization, interpersonal sensitivity, depression, hostility and total average result were lower remarkably than national norm (P ＜ 0.01-0.05). ② Dance-sport and physical diathesis:Among various dances in sport, the highest, lowest and average cardiac rates were (115-194) times/minute, (114-140) times/minute and (138-169) times/minute successively. The amount of exercise is over moderate level and it benefits cardiac vascular system, functions of heart and lung,endurance, etc. Long-term exercise will be good for the physique. ③ Impacts of dance-sport on music cultivation: Before the class, 93.1% of students did not understand musical rhythm or they understood the rhythm,but it is hard for their steps followed the rhythm. After the course of dance-sport, only 2.5% of students did not understand and follow musical rhythm.CONCLUSION: Dance-sports improves not only psychological health and physical diathesis of students, but also their musical and art cultivation.It is mass sports activity, benefiting the health both physically and mentally.

Objective:To induce an anti-hCG? antibody response in reproductive tract and the peripheral by oral inoculation with a recombinant alive Lactobacilli expressing hCG? in different strains of mouse.Methods:The 108,109,1010 Lb.hCG?, a recombinant Lactobacillus expressing hCG?, were inoculated orally into 6-8-week-old female BALB/c and C57BL/6 mice. The immunized mice were all boosted with the same dose at interval of 3 weeks. An indirect ELISA was used to determine anti-hCG? IgG and IgA antibodies in vaginal lavage and serum that were got at the end of 2-8 weeks after the primary immunization. The B and T lymphocytes of spleen, uterus and vagina were prepared respectively, and their proliferation was analyzed by flow cytometry. The numbers of the cells secreting anti-hCG? IgG or IgA antibody were detected by ELISpot.Results:Inoculation of 109 or 1010 alive Lb.hCG? could induce similar response while 108 induce a lower attitude of response. The antiserum of either 109 or 1010 Lb. hCG? immunization after booster could neutralized more than 100 ng/ml of hCG antigen both in BALB/c and C57BL/6 mice. The B cell proliferation of the reproductive mucosa was more significantly higher than that of spleen in BALB/c mice(P

BACKGROUND:In the whole world, spinal cord injuries caused by trauma lead to more than 180 000 people presenting with permanent impairment annualy. A large number of experiments have confirmed in recent years, under physiological conditions, microRNA has specific expression and plays an important role in the nervous system. OBJECTIVE: To discuss the changes in microRNA expression induced by injuries as wel as the pathophysiological significance in spinal cord injury, and to explore the development potential of microRNA in tissue-engineered and clinical repair of spinal cord injury. METHODS:A computer-based search of PubMed and Chinese Journal Database was performed for related articles published from January 2000 to December 2014 using the keywords of “SCI, microRNA, transcriptional control, clinical research progress” in English and Chinese. Finaly, 38 articles were included for result analysis. RESULTS AND CONCLUSION: Mechanical injury initialy triggers a series of complex secondary damages, including nervous, vascular and immune systems, which can influence the severity of spinal cord injury to a great extent. Secondary damage to the spinal cord is mainly attributed to the activation and deactivation of some specific genes associated with celular and biochemical mechanisms, such as cysteine aspartate specific protease (caspase) gene family, apoptosis related protein Fas and its ligand Fasl system, P53 gene, apoptosis related gene Bcl-2 family. Recent studies have proved that the functional activation of microRNA expression is the key to spinal cord injury. With the development of biological information engineering, studies and controling technologies associated with microRNA expression have been gradualy dominated, some clinical application based on microRNA technology has entered the clinical trial stage. It is believed that with the continuous development of technology and decrease of cost, permanent dysfunction due to spinal cord injury can be regulated and repaired through the microRNA technology at gene level in the future.