Orthodontic tooth movement (OTM) is a very complicate mechano-biological procedure which is accomplished by the remodeling of periodontal tissues via orthodontic force.As the connective tissue between alveolar bone and cementum,periodontal ligament plays a vital role in the response and conduction of orthodontic force.The susceptible response of periodontal ligament to orthodontic force during OTM is a critical factor on inducing and modulating periodontal tissue restitution.This article reviews the concrete role of periodontal ligament and its mechano-biological behavior during OTM,discusses its mechanical response and biological mechanism respectively on histological,cellular and moleucular levels.

Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

Reading therapy in hospital libraries, academic libraries and public libraries was described in terms of its theoretical study and practical study,and its significance in hospital library was elaborated by comparative analysis.

Objective:To investigate the clinical efficacy of loratadine combined with compositus mentholi cream in the treatment of infantile with external ear eczema and evaluate the safety .Methods:Totally 96 cases of infantile with external ear eczema were ran-domly divided into the observation group (n=48) and the control group (n=48) according to a random number table .Both groups were given the conventional treatment .The control group was treated with topical compositus mentholi cream , while the observation group was treated with loratadine additionally .Both groups were treated for 10 d.The clinical efficacy , the level changes of interleukin-4 (IL-4), interferon-γ(IFN-γ) and immunoglobulin-E (IgE) before and after the treatment, the incidence of adverse reactions during the treatment and the relapse after 6-month follow-up were compared between the groups .Results:The total effective rate of the obser-vation group (95.83%) was significantly higher than that of the control group (79.17%, P<0.05); the levels of IL-4, IFN-γand IgE in both groups were significantly decreased after the treatment (P<0.05), and those in the observation group were significantly lower than those in the control group (P<0.05);there was no significant difference in the incidence of adverse reactions between the groups (P<0.05); the recurrence rate in the observation group (8.33%) was significantly lower than that in the control group (22.92%, P<0.05).Conclusion:The clinical efficacy of loratadine combined with compositus mentholi cream in the treatment of infantile with external ear eczema is reliable with promising safety .

The WeChat service provided at present in 14 hospitals directly under the Yunnan Health and Family Planning Commission was surveyed with suggestions proposed for providing active Wechat service in hospitals in order to alleviate the difficulty to see a doctor and the tense relationship between doctors and patients.

Objective: To establish a model of rat osteosarcoma in nude mice and to study its biological characteristics.Methods: Intact tumor pieces were inoculated into the right tibia of nude mice.The nude mice were killed at different time and the growth of tumor and metastasis were observed.Result: The tumor growth rate was 100%.From the first week to the second week after implantation it grew into outer tissue from tibia,and widely invaded Soft tissue on the third week.Pulmonary metastasis took place from the fourth week to the fifth week,and the nude mice died of cachexia and respiratory failure resulting from the widely whole pulmonary metastasis on the sixth week or so.Conclusion: The tumor growth rate is high.The model simulates the clinical process of human osteosarcoma,and could be a satisfactory model for studying osteosarcoma.

Objective:To develop an HPLC method for the assay of the content of triptolide in triptolide gel. Methods:The content of triptolide was measured by HPLC on Diamonsil C18 column(250mm?4.6mm i.d,5?m)using methanol-water(46∶54,v/v)as mobile phase,at a flow rate of 1.0ml/min. The UV detection was set at 220nm. Results:The calibration curve was proved to be linear in the range of 10.24~51.20?g/ml with a regression coeficient of 0.999 7. The average recovery of the method was 98.3%;RSD was 0.23%(n=5). Conclusion:This method is found to be rapid and accurate for the measurement of triptolide in triptolide gel.

Objective To explore the treatment measures of gastric mucosal damage caused by gastric acid and ischemia after cerebral trauma. Methods 0 ～5 ℃ colded 5% Sodium Bicarboante solution 250 ml were infused into stomach through nasal gastric tube once and remained at least 30 minutes. Results The total effective rate was 100%. Among them,22 cases(73% )bleeding was stopped by once infusion,8 cases died(27% ) ,but the causes of . the death had no direct relation with IGU. Conclusions The result of the method was definite, satisfied, economical and easy to perform and without sideeffects. It should be the best choice for treating IGU.

Functional gastrointestinal diseases(FGIDs) mainly include functional dyspepsia (FD),irritable bowel syndrome (IBS),functional constipation (FC) and functional abdominal pain syndrome (FAPS).In gastroenterology outpatient,42%～61% FGIDs patients have psychological factors,mostly depression and anxiety.The personality of these patients are almost introvert.Negative life events can be an incentive to lead to depression and anxiety which can aggravate the functional gastrointestinal symptoms.Clinical manifestations include long-term abdominal discomfort,abdominal pain (some with no fixed position),abdominal distension,anorexia,change in bowel habits,retrosternal and upper abdominal burning pain or overlapping symptoms.Some have obvious emotional performance.Dyspepsia symptom score of FD patients with mental disorders are higher than those who have no emotional performance and the gastric emptying decrease greatly than the latter.FGIDs treated by systemic therapy but with no improvement in symptoms may have somatization,compulsion,interpersonal tension,depression,anxiety and other psychological disorders.So psychological evaluation should be carried out to make early recognition for refractory functional gastrointestinal disorders.