Objective To evaluate the expression of p53 in triple negative breast cancer( TNBC) and its impact on the prognosis of TNBC systematically. Methods Documents published on PubMed, Medline,CNKI,CNKI,Wanfang data,and VIP data were screened for relative researches. Two reviewers filtered,extracted and assessed the literatures according to the inclusion criteria and exclusion criteria. The meta analysis was conducted by using RevMan5. 0 software. Result A total 13 trails were included,con-taining 11 Chinese documents and 2 English documents. The meta analysis showed that p53 is significantly expressed in TNBC[OR=2. 02,95%CI(1. 28,3. 19)]. TNBC patients with positive p53 expression had higher rates in lymph node metastasis[OR=2. 02,95%CI(1. 28,3. 19)],recurrence[OR=3. 39,95%CI(1.45,7. 90)],metastasis[OR =2. 65(1. 36,5. 18)],disease-free survival[OR =0. 29(0. 18, 0. 47)]and overall survival[OR=0. 29(0. 18,0. 47)]than patients with negative p53 expression. Con-clusion p53 is overexpressed in TNBC,which plays a certain role in guiding the clinical prognosis of TN-BC. It can be regarded as an independent factor to evaluate the prognosis of TNBC,and a therapeutic tar-get in further studies.

AIM: To establish the method of fingerprinting analysis on Zedoary turmeric oil by GC.Zedoary turmeric oil for the quality control of reference. METHODS: GC method was used to determine the fingerprint and its similarity of Zedoary turmeric oil calculated on Excel and cluster analysis. RESULTS: Seventeen samples of Zedoary turmeric oil indicated that there was a little similarity among them.Based on the cluster analysis,all the Zedoary turmeric oils could be divided into two categories,self-made and market purchasing oil,the latter oils purchased from Liaoning province and Jiangsu province varied considerably.Every category had a common fingerprint mode with clear resemblance. CONCLUSION: When finding the difference between the self-made and the market purchasing Zedoary turmeric oil,quality control requires serious consideration.

AIM: To establish the method of fingerprinting analysis onZedoary turmeric oil by GC. Zedoary turmeric oil for the quality control of reference. METHODS: GC method was used to determine the fingerprint and its similarity of Zedoary turmeric oil calculated on Excel and cluster analysis. RESULTS: Seventeen samples of Zedoary turmeric oil indicated that there was a little similarity among them. Based on the cluster analysis, all the Zedoary turmeric oils could be divided into two categories, self-made and market purchasing oil, the latter oils purchased from Liaoning province and Jiangsu province varied considerably. Every category had a common fingerprint mode with clear resemblance. CONCLUSION: When finding the difference between the self-made and the marketpurchasing Zedoary turmeric oil, quality control requires serious consideration.

BACKGROUND:As a kind of dental implant material, the application of titanium has certain restrictions because of its higher probability of postoperative bleeding rate, infection and gingival hyperplasia. Studies have shown that polymethylmethacrylate has been used in artificial joints and artificial bones, but rarely reported to be used as dental implant material. OBJECTIVE:To explore the biocompatibility indexes such as cytotoxicity, cel adhesion rate, relative cel proliferation rate and post-implantation inflammatory response of human osteoblasts when pure titanium and polymethylmethacrylate are used as dental implant materials, so as to provide certain reference basis for the clinical usage of polymethylmethacrylate as the dental implant material. METHODS:Human osteoblasts were cultured in vitro. Three groups were divided as folows: control group (cels cultured normaly), pure titanium group (cels cultured with titanium extract) and polymethylmethacrylate group (cels cultured with polymethylmethacrylate extract). RESULTS AND CONCLUSION:Compared with the control group, the cel adhesion rate was significantly decreased after 2, 4, 8 and 16 hours of culture with pure titanium and polymethylmethacrylate extracts (P < 0.05); the cel adhesion rate in the polymethylmethacrylate group was higher than that in the pure titanium group (P < 0.05). Compared with the control group, the cels were sparse and grew slowly after 2 days of culture with pure titanium and polymethylmethacrylate extracts. Cels in the polymethylmethacrylate group grew faster with fusiform distribution and obvious drawing phenomenon. Compared with the control group, the relative cel proliferation rate was significantly decreased after 2 days of culture with pure titanium and polymethylmethacrylate extracts (P < 0.05); the relative cel proliferation rate of polymethylmethacrylate group was higher than that of the pure titanium group (P < 0.05). The expression of inflammatory factors in rat serum was significantly increased after 7 days of implantation of titanium and polymethylmethacrylate materials (P < 0.05), the expression of inflammatory factors in the polymethylmethacrylate group was less than that in the titanium group (P < 0.05). There was only one rat developing alergic reaction, but no pyrogen reaction and no death in the polymethylmethacrylate group; and three rats presented with alergic reaction, one rat present with pyrogen reaction and no death occurred in the pure titanium group. These results demonstrate that as the dental implant material, polymethylmethacrylate is superior to pure titanium in the cel toxicity, inflammatory response and biocompatibility.

OBJECTIVE:To provide reference for the individualized treatment of opioid drugs by investigating the effects of OPRM1 A118G gene polymorphism on opioids dosage in Chinese Han Population patients with cancer pain. METHODS:66 Chi-nese Han Population patients with medium and severe cancer pain were selected from a third grade class A hospital. Using fluores-cence in-situ hybridization,OPRM1 A118G genotypes were detected to compare the opioids dosage of different genotype in pa-tients with cancer pain. RESULTS:Among 66 patients,distribution frequency of AA,AG and GG genotype were 36.36%, 53.03% and 10.61%,respectively;those of A and G allele were 62.88% and 37.12%;the daily dosage of opioids in AA,AG, GG genotype patients respectively were(21.67±13.41),(42.00±32.18)and(87.14±73.65)mg,with statistical significance(P<0.001). Among 39 patients receiving opioids for the first time,the dosage of 6 AA genotype patients and 15 AG genotype patients had been adjusted,with statistical significance(P=0.026). After adjusting dosage,daily dosage of AA genotype and AG genotype patients were (16.11 ± 9.16) and(28.57 ± 18.52)mg,with statistical significance (P=0.011). CONCLUSIONS:OPRM1 A118G genotype can influence the dosage of opioid in Chinese Han Population patients with cancer pain,and gene polymorphism can be used as evidence for dosage guide of opioids.

Objective To observe the feasibility and safety of self-made extended endobronchial tube applicated in one-lung ventilation for radical resection of esophageal cancer.Methods 80 patients were randomly divided into two groups:self-made extended endobronchial tube group (group A) and double-lumen endobronchial tube group (group B).The degree of lung collapse,systolic blood pressure,diastolic blood pressure,heart rate,airway pressure (Paw),arterial partial pressure of oxygen(PaO2),arterial partial pressure of carbon dioxide(PaCO2),arterial oxygen saturation (SaO2) were monitored.The time for insertion,rate of successfully intubation for single time,incidence of postoperative hoarseness and sore throat were recorded.Results There were no significant differences in degree of lung collapse,systolic blood pressure,diastolic blood pressure and heart rate,SaO2 in the two groups(all P＞0.05).The comparison of the time for insertion between the two groups:(1.5±0.4)min vs.(2.6±0.8)min(t=4.78,P＜0.05);rates of successfully intubation for single time:95.0％ vs.80.0％(χ2=5.19,P＜0.05).The comparison of Paw between the two groups:T1:(17.3±1.1)cmH2O vs.(22.5±0.9)cmH2O,t=4.613,P＜0.05;T2:(17.9±0.5)cmH2O vs.(23.6±1.4)cmH2O(t=5.438,P＜0.05);T3:(18.1±0.8)cmH2O vs.(24.6±1.2)cmH2O(t=3.741,P＜0.05).The comparison of blood gas during one-lung ventilation between the two groups:PaO2(281.5±53.4)mmHg vs.(187.6±48.8)mmHg(t=5.28,P＜0.05);SaO2(98.1±6.3)％ vs.(94.5±5.7)％(t=3.46,P＜0.05);PaCO2(31.2±3.6)mmHg vs.(37.6±4.1)mmHg(t=6.32,P＜0.05).The comparison of incidence of postoperative hoarseness:1case vs.6cases(χ2=5.91,P＜0.05);The comparison of incidence of sore throat:4cases vs.13cases(χ2=6.13,P＜0.05).Conclusion Single-lung ventilation has achieved via either the double lumen tube or the self-made extended endobronchial tube.However,the self-made extended endobronchial tube is recommended for single-lung ventilation because of the advantages of its easy intubation and position,full oxygenation,low Paw,less incidence of postoperative hoarseness and sore throat.Self-made extended endobronchial tube can be used as a one-lung ventilation method in its indications.

Objective To analyze the effects of overexpressed iASPP on breast cancer cell line MCF-7. Methods The iASPP cDNA was obtained from breast carcinoma tissue by RT- PCR. The breast cancer cell line MCF-7 that overexpressed iASPP was established by stable transfection and G418 selection. The proliferation and apoptosis of the MCF-7 cells that overexpressed iASPP after exposure to cisplatin were detected by MTT and flow cytometry. Results The MCF-7 cells that overexpressed iASPP were successfully constructed. Overexpressed iASPP could significantly decrease the apoptosis rate of MCF-7 cells exposed to cisplatin and weaken the inhibitory effects of DDP on the proliferation of MCF-7 cells. Conclusion Overexpressed iASPP attenuates the sensitivity of human breast cancer cell line MCF-7 to cisplatin.

OBJECTIVE:To extract and separate the water-soluble chemical constitutions from Radix Isatidis.METH ODS:The Radix Isatidis water extract underwent adsorption by D 101 macroporous resin,the portion eluted by ethanol of different concentrations was isolated and purified on silica gel column repeatedly,the physicochemical constants and the spectra data of the compound obtained were determined and its chemical constitutions were identified.RESULTS:3compounds temporarily separated from Radix Isatidis water extract,were identified as:syringin(Ⅰ),indole—3—acetonitrile—6—O—?—D—glucopyranoside(Ⅱ)and(+)—isolariciresinol(Ⅲ),respectively.CONCLUSION:For the first time,compound(Ⅰ)and compound(Ⅱ)were isolated from Radix Isatidis.

OBJECTIVE:To isolate the chemical constituents from Radix isatidis.METHODS:The 95% ethanol extract of Radix Isatidis underwent adsorption by silica gel,and the portion eluted by different solvents was isolated and purified on silica gel column repeatedly.The physico-chemical constants and spectral data of the obtained compounds were determined and their chemical structure was identified.RESULTS:6 compounds were separated from chloroform and n-Butanol parts of Radix Isatidis and identified as:cholesterol(Ⅰ),indigotin(Ⅱ),indirubin(Ⅲ),p-Hydroxybenzaldehyde(Ⅳ),salicylic acid(Ⅴ),lariciresinol-4,4'-2-O-?-D-glucopyranoside(Ⅵ),respectively.CONCLUSION:For the first time,compound(Ⅰ)and compound(Ⅳ)were isolated from Radix Isatidis,this finding serves as reference.

OBJECTIVE:To study the main factors affecting in vitro dissolubility of total puerariae flavones(TPF)bioad?hesive tablets.METHODS:Using HPMC,Carbopol(CP934NP)as bioadhesive and base materials,lactose as porogenic agent to prepare bioadhesive tablets;Basket-rotating method was adopted to determine the dissolubility while0.1mol/L HCl was used as dissolution medium,Rotational speed was100r/min.The accumulated dissolution was detected and the influence of the amount of HPMC,CP,kind of porogenic agent,amount of lactose,size of granules in pressed tablets and medium pH on dissolubility was observed.RESULTS&CONCLUSION:The amount of HPMC,CP and lactose,kind of porogenic agent,size of granules in tablets and medium pH can affect the dissolubility of bioadhesive tablets.