Objective To investigate the correlation between VEGF expression and MVD,clinicopathological features in bladder urothelial carcinoma.Methods Immunohistochemical SP method was used to detect VEGF in 54patients with primary bladder urothelial carcinoma and then counted the MVD in tumor,the relationship between VEGF expression and MVD was observed.Results The positive expression rate of VEGF in 54 patients with primary bladder urothelial carcinoma was 61.1％(33 cases),while there was no expression in normal bladder mucosa.MVD count of VEGF-positive group was significantly higher than VEGF-negative group,the difference was statistically significant(P ＜ 0.05),and along with the enhanced of VEGF expression,MVD count increased significantly,the difference was statistically significant(P ＜ 0.05); the expression of VEGF in cancer tissues enhanced significantly along with the increase of tumor grade,TNM stage and occurrence of lymph node metastasis(P ＜ 0.05).Conclusion The positive expression of VEGF was related with MVD and tumor malignant processes in bladder cancer,and it could provide valuable markers for the prognosis of patients.

(-)-Epigallocatechin-3-gallate (EGCG),a natural medicine with anti-neoplastic activity,significantly prevent cancers via inhibiting tumor cell proliferation,suppressing angiogenesis and promoting apoptosis,etc.Furthermore,the new discoveries about anti-tumor mechanisms of EGCG in digestive system,reproductive system and respiratory system shorten the course of EGCG application in clinical.

OBJECTIVE:To investigate the characteristics of drug release and the factors affecting the in vitro dissolubility of diclofenac potassium double-layer tablets(DPD).METHODS:UV-spectrophotometry and rotating basket method in the pha-rmacopeia of China 2000 edition were used to determine the in vitro dissolubility of DPD and Higuchi equation was adopeted to simulate the in vitro drug release.The main parameters of dissolution were stastistically analysed.RESULTS:Dissolution parameters of DPD were as follows:T0.3=0.10h,Td=3.30h,T0.9=9.19h.Hardness of tablets did not affect the dissolution rate significantly;pH of dissolution media significantly influenced on the rate.Rotation speed had a significant effect on dissolution action only at the beginning of test.CONCLUSION:DPD has good properties of fast and sustained release.Proper media is the key of in vitro drug release test,however,the ultimate results should be based on in vivo trial.

Objective To explore the expression levels of HIF-1 α and MUC5AC,MUC5B mRNA in chronic rhinosinusitis(CRS) and their correlation.Methods Eighty cases of CRS with nasal polyps(CRSwNP) treated in our hospital from May 2014 to May 2015,80 cases of CRS without NP(CRSsNP) and 80 healthy people without CRS(control group) were chosen.Nasal sinus mucosa in 3 groups were collected and treated.The expressions of HIF-1 α,MUC5AC and MUC5B mRNA were measured by semiquantitative reverse transcription polymerase chain reaction(RT-PCR),and their correlations were analyzed.Results The relative expression levels of HIF-1 α,MUC5AC and MUC5B mRNA in the CRSwNP group or CRSsNP group were 1.35±0.85,1.35±0.91;0.80±0.55,0.79±0.49 and 1.18±1.01,1.21±1.02 respectively,while which in the control group were 0.42±0.33,0.43±0.36 and 0.47±0.43 respectively,the difference between the CRS groups and control group was statistically significant(P<0.05).The expression of HIF-1αin the CRSwNP group and CRSsNP group was positively correlated with MUC5AC,MUC 5B mRNA(r=0.476,P=0.023,r=0.476,P=0.026,r=0.478,P=0.035,r=0.508,P=0.021).The ROC analysis results showed AUC=0.956 4.Conclusion The expression levels of HIF-1α and MUC5AC,MUC5B mRNA in chronic rhinosinusitis are significantly upregulated,moreover HIF-1α is closely related with MUC5AC and MUC5B mRNA.

BACKGROUND: Systematic family therapy focuses on the interaction,and group counseling contributes to the improving of interpersonal relationship. To introduce the ideas and techniques of systematic family therapy into school situations, which is necessary to the peer relationship improvement of disliked children in the class.OBJECTIVE: To study the feasibility of introducing the ideas and techniques of systematic family therapy into school situations with the aim of improving the peer relationship.DESIGN: Inequality experiment group and control group design.SETTING: Institute of Educational Sciences and Administration, Yunnan Normal University.PARTICIPANTS: The experiment was carried out in Kunming No.l Primary School from September 2000 to April 2001. Two out of three ordinary classes in Grade Six were chosen to take part in the experiment at the school. Both classes were similar in performance, total number and composing. Class 3 of Grade 6 (n=46) was made experiment class randomly,and Class 1 of Grade 6 (n=41) was comparison class. Children of both classes were measured by the Social Distance Scale for Children at the same time, which was described in 5 grades. All children were required to evaluate their classmates by the list. The following 5 grades respectively scored 5, 4, 3, 2 and 1 point: making best friend with him or her willingly,hope to be with him or her in the same class but not the best friend, being with him or her willingly but not many times or too long, he or her may be in our class but have no intercourse, hope him or her not to be in our class. The evaluation result was statistically analyzed, and the higher the mean score of child had, the more popular he was. Five male children who was the farthest from their peers in the experiment class were taken as the experiment group according to the score of Social Distance Scale for Children, who were 12-13 years old with a mean age of 12.7 years. Another 5male children in the other class were selected as the control group by the same way, who were 12-13 years old with a mean age of 12.5 years.METHODS: The idea of systematic family therapy was introduced into the experiment group, including 10 times of group counseling, which were carried out by games, role-playing, group discussion, brain-storming etc.The "everyday work" was assigned to the class as the techniques of systematic family therapy required, especially for children in the experiment group such as "keep secret progress account", and teachers and classmates were required to watch and note their members' progress secretly.While children in the control group and corresponding class were given no intervention measure. Children of both classes were tested by the Social Distance Scale for Children to evaluate the changes in peer relationship during and after the experiment respectively. And children in the experiment group were required to fill in the Group Activity Viewpoint Questionnaire.MAIN OUTCOME MEASURES: Comparison in score of Social Distance Scale for Children before, during and after the intervention, and the investigation on viewpoint about group activity in the experiment group.RESULTS: A total of 10 children in both groups were involved in the analysis of results. There was no significant difference between children of the two groups in social distance before intervention and during it(t=0.232,1.089, P ＞ 0.05). It cotld be Iearnt from the experiment after test that the score in children of the experiment group was remarkably higher than that of the control group (t=3.169, P ＜ 0.05). The effect of group activity on children in the experiment group was remarkably clear and excellent.CONCLUSION: It is effect and feasible to introduce the idea and techniques of systematic family therapy into school situations, aiming at improving the peer relationship of children.

ObjectiveTo study the expression of growth factor receptor binding protein 2(GRB2) in bladder transitional cell carcinoma (BTCC),and clarify the role of GRB2 in the development and progression of BTCC.MethodsThe GRB2 expression of BTCC tissue from 60 patients who first got BTCC (BTCC group) and adjacent normal bladder from 30 patients(control group) were detected by SP immunohistochemical method.ResultsThe GRB2 positive expression rate of BTCC group [81.7％(49/60)] was significantly higher than that of control group [30.0％(9/30)] ( X2 =12.122,P ＜0.05).According to tumor histological grade,the GRB2 positive expression rate was grade Ⅰ[66.7％( 16/24)] ＜gradeⅡ [89.3％(17/19)] ＜grade Ⅲ [94.1％(16/17)],grade Ⅰ and grade Ⅱ,Ⅲ had significant difference (P ＜0.05),but grade Ⅱ and grade Ⅲ had no significant difference(P ＞0.05).The GRB2 positive expression rate in the clinical stage was stage T1 [63.0％ (17/27)]＜ stage T2 [95.7％ (22/23)] ＜ stage T3[ 100.0％(10/10) ],stage T1 and stage T2,T3 had significant difference (P ＜ 0.05 ),but stage T2 and stage T3 had no significant difference (P ＞0.05).The GRB2 expression among different gender and age had no significant difference (P ＞0.05).ConclusionsGRB2 has high expression in BTCC tissues.GRB2 expression is related to tumor histological grade andclinical stage.GRB2 plays an important role in the process of BTCC cell proliferation and differentiation.

A young female complained repeated nasal discharge for over three months with discomfort of right cheek, and oral antibiotics had less effect. She has a history of "root canal therapy" five years before. Physical examination found purulent secretion in the right middle nasal meatus, and light tenderness in the right side of the maxillary sinus area. The CT scan of paranasal sinus shown possible fungal infection of right maxillary sinus. Finally the nasal endoscopic surgery confirmed the fungus ball of right maxillary sinus with foreign body (the root canal filling material).
Endoscopy ; Female ; Foreign Bodies ; diagnosis ; Humans ; Maxillary Sinus ; microbiology ; Maxillary Sinusitis ; microbiology ; Mycoses ; diagnosis ; Respiratory Tract Infections ; microbiology ; Tomography, X-Ray Computed

OBJECTIVE:To investigate the regularity and characteristics of ADR induced by Mailuoning injection,and to provide reference for rational use of drugs.METHODS:In retrospective study,ADR/ADE cases of Mailuoning injection reported in Henan province from 2004 to 2009 were analyzed statistically.RESULTS:Main clinical manifestations were lesion of skin and its appendents(24.95%),respiratory system injury(23.81%) and nervous system injury(15.38%).The clinical manifestations of serious ADR/ADE were allergic shock,dyspnea and choking etc.CONCLUSION:Clinical rational use of drugs should be improved to avoid serious ADR and guarantee the safety of drug use.

Objective To determine whether sleep structures,the waveform components of NREM and rapid eye movement (REM) sleep, and the relationship between sleep variables and mental status are altered. Methods Totally 16 Alzheimer's disease(AD) patients and 16 age-matched controls were studied for two consecutive nights.All cases were video-monitored during the recordings of electroencephalography,electro-oculography,electrocardiography,electromyography in chin,and nasal airflow. Results (1) Compared with normal controls,duration of total sleep ( F =6.30, P =0.017 9),stage Ⅱ sleep ( F =16.03, P =0.000 4),and REM sleep ( F =9.84, P =0.002 1) were remarkably decreased,but waveform components of slow-wave (stage Ⅲ-IV) sleep ( F =11.50, P =0.004) were increased in AD patients.(2) Sleep spindle duration and density,and its relative and absolute power,and K complex density ( F =13.64～79.11, P

Objective In order to evaluate the curative effects and mechanism of Ulinastatin on acute pancreatitis(AP). Methods 40 patients with AP were enrolled in this prospective study. They were comparable in dispearing time of symptoms and physical signs and laboratory data. Results The symptoms and physical signs of patients were significantly relieved in Ulinastatin group,and the labrotory data,such as the blood amylase and urine amylase recovered normal after five days with Ulinaststin. Conclution Ulinaststin has good clinical value in AP.