Objective To explore the clinical effect of blood perfusion combined with penehyclidine hydrochloride injection in the treatment of acute severe organophosphorus pesticide poisoning.Methods 58 patients with severe organophosphorus pesticide poisoning were selected as the research subjects.By using digital table method,they were randomly divided into observation group and control group,29 cases in each group.The two groups were given conventional treatment and blood perfusion treatment after admission,the observation group was treated with penehyclidine hydrochloride injection on this base.Before and after treatment,the myocardial enzymes,liver,kidney function index,blood gas analysis,cholinesterase activity,as well as other treatment of the two groups were observed,and the results were compared.Results Compared with the control group,after treatment,the myocardial enzyme,transaminase,serum amylase and creatinine levels in the observation group were significantly lower,cholinesterase level was significantly increased,the oxygen partial pressure increased,the partial pressure of carbon dioxide decreased obviously,the differences were statistically significant(all P<0.05).The hospitalization time and cholinesterase activity recovery time of the observation group were significantly shorter than those of the control group[(12.57±2.14)d vs.(16.35±2.73)d,(5.46±2.29)d vs.(7.61±3.47)d],and the cure rate of the observation group was significantly higher than that of the control group(100.00% vs.82.76%),the differences were statistically significant(t=2.139,2.208,x2=5.472,all P<0.05).Conclusion Blood perfusion can directly adsorb organophosphorus pesticide,shorten the recovery time of cholinesterase and reduce the case fatality rate,and it combined with penehyclidine hydrochloride injection not only can improve the clinical curative effect of blood perfusion,but also can protect the organ.

Objective:To measure serum level of cystatin C (Cys C)in patients with essential hypertension (EH)and analyze its correlation with pulse pressure (PP).Methods:A total of 60 EH patients and 30 cases with normal physi-cal examination results were selected.According to PP level,EH patients were divided into PP>60mmHg group (n=29)and PP≤60mmHg group (n=31).Serum Cys C concentration was measured by latex particle-enhanced immu-noturbidimetric method in two groups,and the results were statistically analyzed.Results:Cys C level of PP >60mmHg group was significantly higher than that of PP≤60mmHg group,and the both groups were significantly higher than that of normal control group [(1.40±0.06)mg/L vs.(1.19±0.54)mg/L vs.(0.72±0.20)mg/L], P <0.05 all;linear correlation analysis indicated that Cys C level was positively correlated with PP (r =0.325,P <0.05)and systolic blood pressure (SBP,r = 0.399,P < 0.05),and PP was positively correlated with SBP (r =0.876,P <0.01)in EH group.Conclusion:Cys C level is positively correlated with PP in EH patients.Cys C level can be used as an early index detecting renal function damage caused by hypertension.

Objective:To introduce a method and theory about how implementation vein image projection epidermis and conduct the clinical testing.Methods: Base on existing electronic components construct circuit. The construct circuit was made of industrial personal computer, mini-projector, camera and so on. The image is captured by camera transfer to industrial personal computer. Choosing suitable image process algorithms modify image. Processed image that storage in industrial personal computer transfer to mini-projector, vascular vein lines is produced from mini-projector.Results: Through this way that vascular vein projector method get image basic agreement vascular trend, meet the clinical needs.Conclusion: Vein image projection methods meet clinical need can be used to study other field.

Objective To explore the mutual effect of proinflammatory factor tumor necrosis factor(TNF)-? and cigarette smoke extract(CSE) in the induction of mucin 5AC in BEAS-2B human airway epithelial cells.MethodsThe BEAS-2B cells were transfected with nuclear factor(NF)-?B decoy oligonucleotides(ODNs) or transfected with NF-?B scrambled ODNs as negative control,then treated with TNF-?,CSE,and TNF-? plus CSE,respectively. The expression of phosphorylated epidermal growth factor receptor(p-EGFR),mucin 5AC(MUC5AC) protein production and gene expression were detected by Western blot,ELISA and RT-PCR respectively.Results Significant increasing of p-EGFR protein production in scrambled ODNs transfected cells with elevation of MUC5AC protein production and mRNA expression was recorded and TNF-? and CSE co-incubated groups were much higher than single TNF-? or CSE stimulated group(P

Objective To investigate clinical characteristics of paediatric allergic rhinitis .Methods 133 paediatric patients with allergic rhinitis were divided into preschool group and school-age group ,and analyzed the clinical characteristics .Results The pro-portion between preschool group and school-age group with nasal discharge ,nasal blockage ,associated with coughing ,preschool group has significantly difference with school-age group(P<0 .05);the proportion between preschool group and school-age group with mild intermittent ,moderate to severe persistent ,preschool group has significantly difference with school-age group(P<0 .05);The proportion of dietary factors between two group is significant (P<0 .05) .The proportion of relation between intermittent ,mild persistent ,environmental change ,other ,affect morpheus quality ,difficult to concentrate ,and cognitive decline between the two had no statistical significance (P> 0 .05) .Conclusion Compared the clinical manifestation between preschoolers and school-age chil-dren ,we got some clinical data of paediatric allergic rhinitis patients ,those were beneficial to the diagnose and therapy of paediatric allergic rhinitis .

Early and non-invasive hematological test in breast cancer is always hot topic. In recent year, enzyme, cytokine, glycoprotein, carcinoembrynoic antigen (CEA), prolactin and oncogene protein are studied more, among them CA15-3 and CEA have important clinical value. Looking for hematological test markers which have high sensitivity and speciality is important work.

Objective To clone the human mucin (MUC)5AC gene promoter and construct its luciferase reporter vector for human MUC5AC gene and analyze its transcriptional activity. Methods The 1 348 bp DNA sequence at the human MUC5AC gene 5 end was analyzed by the Vector NTI software.After the target sequence from human A549 cells genomic DNA was amplified by PCR method, and the product of PCR was sequenced.By promoter deletion analysis, 3 promoter segments with diferent lengths were amplified by PCR, then the products were identified by DNA sequencing, and 4 promotor segments were inserted into pGL3- enhancer vectors.Site-specific mutagenesis technique was used to establish mutants of specificity protein (SP)-l and nuclear factor-kappa B (NF-кB) site in MUC5AC gene promoter. The relative luciferase activities were detected in the transfected A549 cells. Results Sequence analysis indicated that there were many cis-acting elements in the regions of 1 348 bp DNA sequence at the human MUC5AC gene 5 end.The 4 reporter gene vectors with promoter segments with different lengths were constructed successfully.Dual-luciferase assay revealed the 372 bp fragment including activity with the minimal fragment. Neutrophil elastase (NE) could increase the expression of luciferase reporter gene plasmid containing mutated NF-кB version (P＜0.05 vs. contro1) of MUC5AC promoter in the transfected A549 cells. The induction by NE decreased markedly when the SP-l element in MUC5AC promoter were mutated. Conclusion This research may provide an important basis for the further study of human MUC5AC gene promoter activity and regulation of gene expression.There is an up-regulative element of gene transcription in the region of -324 to -64 bp in MUC5AC gene upstream. SP-l site of the promotor mediates NE-induced MUC5AC expression in human A549 cells.

The concrete methods and experiences for foreign students in case study in the past decades were introduced in this article. We emphasized on teaching students in accordance with their aptitude and step by step, explaining the profound things in a simple way and giving attention to different students' capabilities during teaching. In the practice, we surely felt that case study will bring more advantage than traditional teaching methods, motivate students and improve the capabilities to understand the course content.

OBJECTIVE:To evaluate the effects of loading-dose rosuvastatin on vascular endothelial function in patients with non-ST segment elevation acute coronary syndromes (NSTE-ACS) after early interventional therapy. METHODS:Totally of 128 NSTE-ACS patients underwent early interventional therapy were randomly divided into conventional dose group (63 cases) and loading dose group(65 cases). Before operation,all patients were given Clopidogrel sulfate tablets 300 mg and Aspirin enteric-coat-ed tablets 100 mg;on this basis,conventional dose group was given Rosuvastatin calcium tablets 10 mg orally;loading dose group was given Rosuvastatin calcium tablets 20 mg orally. After PCI,both groups were given Rosuvastatin calcium tablets 10 mg orally, qd,and Aspirin enteric-coated tablets(100 mg/d)and Clopidogrel sulfate tablets(75 mg/d),for consecutive 3 months. The blood samples were collected before surgery,8 h and 24 h after surgery. The serum levels of CK-MB,cTn T,hs-CRP,ET and NO were detected. The occurrence of major adverse cardiovascular events was recorded within 3 months after surgery. RESULTS:Compared with before surgery,the serum levels of CK-MB,cTn T,hs-CRP and ET were increased significantly 8 h and 24 h after surgery, while the level of NO was decreased,with statistical significance (P<0.05). However,the change range of loading dose group was lesser than that of conventional dose group,with statistical significance(P<0.05). There was no statistical significance in the incidence of adverse cardiovascular events between 2 groups(P>0.05). CONCLUSIONS:For NSTE-ACS patients underwent ear-ly interventional therapy,loading dose of rosuvastatin can protect the patients and inhibit the injury of vascular endothelial cell in-duced by the surgery.

Objective To explore the mutual effect of proinflammatory factor tumor necrosis factor (TNF)-α and cigarette smoke extract(CSE) in the induction of mucin 5AC in BEAS-2B human airway epithelial cells. Methods The BEAS-2B cells were transfected with nuclear factor(NF)-κB decoy oligonucleotides(ODNs) or transfected with NF-κB scrambled ODNs as negative control,then treated with TNF-α,CSE,and TNF-α plus CSE, respectively. The expression of phosphorylated epidermal growth factor receptor (p-EGFR) , mucin 5AC( MUC5AC) protein produc-tion and gene expression were detected by Western blot, ELISA and RT-PCR respectively. Results Significant in-creasing of p-EGFR protein production in scrambled ODNs transfected cells with elevation of MUC5AC protein production and mRNA expression was recorded and TNF-α and CSE co-incubated groups were much higher than single TNF-α or CSE stimulated group(P <0. 05). In NF-κB decoy ODNs transfected cells,TNF-α stimuli revealed an obvious reduction of MUC5AC protein production and gene expression. Conclusion TNF-α and CSE can syner-gistically induce mucin 5AC expression in BEAS-2B cells, transcription factor NF-κB plays a critical role in TNF-α induced mucin production but not as so important in mucin production by CSE stimulated cells.