Polycythemia vera (PV) is a chronic clonal myeloproliferative disorder of hematopoietic stem cell characterized by a pronounced symptom burden, including fatigue, pruritus, and symptomatic splenomegaly, along with an increased risk of thrombosis and the potential for evolution to myelofibrosis (MF) and secondary acute myeloid leukemia. Research on pathogenesis, diagnosis and treatment of PV has made a great progress since the discovery of JAK2 V617F mutation in 2005. The change of epigenetics plays an important role in the progression of PV, and the drugs for apparent genetic alteration may improve the life quality of PV patients.

With the fast social and economic development ecosystem has been seriously impacted. Country ecotypic sanitary system based on urine diverting Ecosan toilets and biogas Ecosan toilets can make positive contributions to the protection of ecosystem. This paper makes a review on the conception of ecotypic sanitary system the application of two types of ecotypic utilities the effect of ecotyptic sanitary utility and disease prevention and control comprehensive benefit of country ecotypic sanitary system and construction and operations. The results of researches demonstrate that although some valuable achievements have been gained in those fields mentioned above further improvement should be made and the application should be further widened as soon as possible.

[Summary] Nowadays ,obesity and diabetes mellitus have become an important worldwide public health problem. Bariatric surgery is an effective treatment method of morbid obesity and type 2 diabetes mellitus (T2DM ). Although nutrition‐related complications after surgery is relatively common ,these complications can be detected and prevented. The key is to establish a routine perioperative prophylaxis and postoperative long‐term monitoring system. Routine metabolic and nutritional monitoring is recommended after all bariatric surgical procedures ,which can help patients maintain long‐term weight loss ,timely detect and treat surgery‐related complications and improve clinical outcomes. In the present mini review ,the perioperative and postoperative nutrition management was introduced.

Objective To establish a quality control for Tianmatoufengling Capsule. Methods HPLC method was taken for the determination of Gastrodine in Tianmatoufengling Capsule,A Kromasil C18 column was used,with methanol 0.05% Calcium phosphace(2.5∶97.5) as the mobile phase,and its flow rate was 1.0 mL/min. The detection wavelength was 220 nm. TLC method was used for the identification of Tianmatoufengling Capsule. Results Under the used chromatographic condition,Tianmatoufengling had fine linear responses(r =0.999 9) in the concentration range of 6.016～96.256 ?g/mL,the average recovery was 100.02%,RSD was 2.24%. The method used in TLC-qualitative analysis was simple and sensitive with high specificity,four ingredients identified in Tianmatoufengling Capsule could be detected and all showed specific spots. Conclusion The method employed in this study was convenient and reliable with good repetition. It can be used for quality control in production of Tianmatoufengling Capsule.

Objective Based on the result that a gene coding for human HSS had been identified and prokaryotically expressed, this study is aiming to investigate the proliferative and protective effect of recombinant HSS on hepatoma cells. Methods hHSS protein was produced in BL\|21 strain of E.Coli containing pET\|42a vector and purified with His\5Tag affinity chromatography. A various dosages of 80\|400??g/L were administrated into cell culture. The cell proliferation was analyzed by MTT, cell\|count and flow cytometry methods. Furthermore, cellular growth signaling as indexed by phosphorylation of mitogen\|activated protein kinase (MAPK) was determined with Western blot. In addition to cell growth, protection of hHSS on the cells against H\-2O\-2 was also observed. Results It was showed that recombinaht HSS of 400??g/L was able to promote DNA synthesis by 21\^5% as compared to non\|treated cells. After 24?h of HSS action, the cell division measured by MTT method as well as cell\|count was significantly enhanced. Meantime, it was indicated that the phosphorylation of MAPK Thr202/Tyr204 was increased in 79\^0% as compared with that of the non\|treated cells. Pretreatment of the cells with hHSS for 12?h prior to H 2O 2 injury preserved the survival of them.Conclusion It is postulated that hHSS is an active protein to stimulate liver proliferation. [

OBJECTIVE:To establish the fingerprints of 11 batches of Cardiospermum halicacabum from Guangxi,and detect its spectrum-effect relationship of antioxidant activity in vitro,and provide reference for revealing the material basis for its antioxi-dant activity. METHODS:HPLC was conducted to establish the fingerprints of 11 batches of C. halicacabum from Guangxi,the 1, 1-diphenyl-2-trinitrobenzene hydrazine(DPPH)free radical scavenging method was used to determine the antioxidant activity in vi-tro of C. halicacabum from different origins,and bivariate correlation analysis was adopted to detect the spectrum-effect relation-ship of antioxidant activity in vitro. RESULTS:The similarities of 11 batches of C. halicacabum from Guangxi were all more than 0.9,and the content change of peak 2(protocatechuic acid),3,4,12 in 16 common peaks was closely related to the DPPH free radical scavenging activity. CONCLUSIONS:The chemical composition of C. halicacabum from different origins in Guangxi is ba-sically consistent,while there are some differences in the quality of the producing area. The protocatechuic acid (peak 2) and the unknown compounds represented by peak 3,4,12 may be the pharmacodynamic material basis of its antioxidant activity.

Objective To survey the status and influence factors of radiology department nurses self-protection behavior. Methods The radiology department nurses′self-protection behavior questionnaire compiled by the researchers were used to conduct a questionnaire survey of 100 nurses in different grade hospitals. Results Scores of the item body fluid of patients with contact contaminated were wearing glovesof second-class hospital and third-class hospital were (3.695±0.73) points and (3.73±0.74) points, and there was no statistically significant different between them (P> 0.05). The other items about self-protective behavior of nurses from different levels of hospitals had some differences (t=-4.24-2.43, P<0.05). Conclusions Self-protective behavior of radiology department nurses is affected by many factors. Nursing managers should take effective measures to improve nurses′self-protective consciousness and ability.

Adrenocorticotropic Hormone ; therapeutic use ; Brain-Derived Neurotrophic Factor ; physiology ; Child, Preschool ; Female ; Humans ; Methyl-CpG-Binding Protein 2 ; genetics ; Mutation ; Rett Syndrome ; drug therapy ; genetics

Objective To investigate the distribution and antimicrobial resistance of clinically isolated pathogens from geriatrics department of a hospital in 2013,so as to provide reference for clinical antimicrobial use.Methods Distribution and antimicrobial resistance of pathogens isolated from patients in a geriatrics department between Janu-ary and December 2013 were analyzed statistically.Results Of 1 896 pathogenic strains,1 289(67.99%)were gram-negative bacteria,439 (26.00%)were gram-positive bacteria,and 114(6.01%)were fungi;the top 4 isolated pathogens were Klebsiella pneumoniae ,Pseudomonas aeruginosa ,Acinetobacter baumannii ,and Escherichia coli .Extended-spec-trumβ-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae accounted for 53.26% and 31.10% of Escherichia coli and Klebsiella pneumoniae respectively;Of Staphylococcus aureus and Staphylococcus epidermidis strains,methicillin-resistant isolates (MRSA and MRSE)accounted for 22.47% and 80.00%,respectively,of Enterococ-cus strains,vancomycin-resistant isolates (VRE)was 3.10%,Klebsiella pneumoniae and Enterobacteriaceae were highly sensitive to imipenem,meropenem and ertapenem.Resistant rate of Acinetobacter baumannii to imipenem and meropenem was 79.48% and 80.35% respectively,Pseudomonas aeruginosa had the lowest resistant rate to amikacin (10.70%). Gram-positive bacteria were highly sensitive to vancomycin and linezolid.Conclusion The major pathogens isolated from the elderly patients are gram-negative bacteria,and antimicrobial resistance is serious,surveillance of antimicrobial resist-ance is important for rational use of antimicrobial agents and control of pathogen resistance.

Objective To evaluate the CT and MRI imaging features of primary ventricular lymphoma ,so as to provide a reference for the diagnosis and differential diagnosis of the disease .Methods The clinical data and imaging findings of primary ventricular lymphoma in one case was retrospectively analyzed with literature review and compared with primary lymphoma in parenchymal brain .Results Slightly high density tumor presented with regular shape in the trigone of lateral ventricles on CT findings , and slightly low signal intensity on T 1 and slightly high signal intensity on T 2 appeared on MRI findings with small cystic necrosis in tumour .An enhancement scan showed obviously enriched, and there was perilesional edema.After 14 day’s dehydration treatment, rechecked indicated that the lesions were significantly en-larged, the shape were irregula , the area of cystic necrosis was enlarged , heterogeneous enhanced scanning were enhanced , perilesional ede-ma became more heavier , and the imaging feature of “closed fist” appeared .It was proved to be diffuse large B cell lymphoma with surgical pathologic examination , and there was no lymphoma in other regions of the body with whole body PET-CT examination , which was clinically diagnosed as primary ventricular lymphoma .Conclusion Primary ventricular lymphoma is rarely reported ,and it has similar imaging mani-festations with primary lymphoma in parenchymal brain .The combination of multiple imaging modalities will be helpful to diagnosis and dif -ferential diagnosis for the suspected patients .