Epilepsy ; diagnosis ; drug therapy ; surgery ; Humans ; Magnetoencephalography ; Positron-Emission Tomography ; Stereotaxic Techniques ; Surgery, Computer-Assisted ; methods ; Treatment Failure

Humans ; Infant ; Job Syndrome ; diagnosis ; immunology ; therapy ; Male

Animals ; Antibodies, Monoclonal ; metabolism ; therapeutic use ; Biomarkers, Tumor ; metabolism ; Breast Neoplasms ; metabolism ; pathology ; therapy ; Drug Delivery Systems ; Epidermal Growth Factor ; immunology ; metabolism ; physiology ; Epithelial-Mesenchymal Transition ; GPI-Linked Proteins ; Humans ; Immunotherapy ; methods ; Intercellular Signaling Peptides and Proteins ; Membrane Glycoproteins ; immunology ; metabolism ; physiology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Proteins ; immunology ; metabolism ; physiology

Objective To explore the effect of health education and psychological nursing on prevention of bladder cancer recrudesce.Methods 168 cases of patients suffered from bladder cancer were divided separately into 86 cases as the observation group and 82 cases as the control group at random.The observation group was given surgery and routine treatment as well as health education and psychological nursing intervention.The control group received only surgery and routine treatment.The SAS and SDS were applied to measure psychological situation of the patients of the two groups and rate of bladder cancer recrudesce was also compared after taking health education and psychological intervention.Results The anxiety and depression score were lower,the recrudesce rate of tumor was decreased in the observation group than the control group.Conclusions Health education and psychological nursing can eliminate psychological barrier of the bladder cancer patients suffered from anxiety and depression,in-tensify the treatment confidence,improve recovery,increase living quality and prevent tumor recrudesce.

Objective To explore the clinical efficacy and safety of bevacizumab combined with docetaxel in treatment of advanced cervix cancer.Methods Seventy-five patients with advanced cervix cancer accepted in our hospital from April 2011 to April 2016 were selected and divided into observation group with 43 cases and control group with 32 cases according to different treatment methods.Patients in control group were given docetaxel,and patients in observation group were combined with bevacizumab on the basis of control group.The clinical efficacy,adverse reactions,life quality and pharmacoeconomics of two groups were observed and compared.Results The total efficacy and clinical control rate of observation group were all higher than control group with statistically significance (P ＜ 0.05).The adverse reaction rate of two groups had no difference.Total efficacy of life quality improvement was 83.72％,which was obviously higher than control group 62.50％ with statistically significance (P ＜ 0.05).The average cost of observation group was (83 ± 10) thousands,of control group was (18± 6) thousands.The cost of observation group was obviously higher than control group with statistically significance (P ＜ 0.05).Conclusion Using bevacizumab combined with docetaxel in treatment of advanced cervix cancer has better effect clinical but costs much than docetaxel.We should set on treatment according to patients' own situation.

Objective To investigate early kidney injury in the patients with non-alcoholic fatty liver disease (NAFLD) without hypertension,diabetes and history of kidney diseases,as so to provide evidence for preventing early kidney injury in patients with NAFLD.Methods From December 2014 to January 2016,169 subjects visiting Sichuan Provincial People's Hospital were recruited.Among them,104 cases were in NAFLD group,31 cases were in simple obesity group (overweight or obesity),and 34 subjects were in the healthy control group.The general data,biochemical indexes,metabolic indexes,biochemical indexes of kidney,and early kidney injury makers,including serum β2-microglobulin,urinary albumin and creatinine ratio (ACR) and estimated glomerular filtration rate (eGFR),were detected.Least significant difference-t test,chi-square test and Spearman correlation analysis were performed for statistical analysis.Results Compared with simple obesity group,alanine aminotransferase (ALT),aspartate aminotransferase (AST),γ-glutamyl transpeptadase (GGT),fasting insulin level,homeostasis model assessment-insulin resistance (HOMA-IR),serum creatinine,ACR and β2 microglobulin were higher in NAFLD group ((21.13 ± 8.14) U/L vs.(66.20 ± 44.34) U/L,(24.80 ±9.57) U/Lvs.(49.78 ±25.41) U/L,(19.26 ±7.88) U/Lvs.(66.53 ±56.34) U/L,(7.03± 1.52) mU/Lvs.(9.55 ±5.41) mU/L,1.22 ±0.38 vs.2.23 ±2.01,(62.90 ± 10.01) μmol/L vs.(71.75 ±10.80) μmol/L,(4.41 ±1.16) μg/mg vs.(13.76 ±9.56) μg/mg,(1.46 ±0.26) mg/L vs.(2.01 ±0.53) mg/L);however the eGFR was lower (112.46 ±11.90) mL · min-1 · (1.73 m2) 1 vs.(101.09± 17.17) mL · min-1 · (1.73 m2)-1).The differences were statistically significant (t =9.825,8.250,8.288,4.229,4.121,4.007,9.732,7.792 and-3.443,all P ＜ 0.01).There was no statistically significant difference in the remaining indicators (all P ＞ 0.05).Compared with healthy control group,the renal injury indexes serum creatinine,ACR and 32 microglobulin of NAFLD group were higher ((58.78 ± 7.77) μmol/Lvs.(71.75±10.80) μmol/L,(1.01 ±0.32) μg/mg vs.(13.76 ±9.56) μg/mg,(1.12 ± 0.15) mg/L vs.(2.01 ±0.53) mg/L),and the eGFR was lower ((115.10 ± 12.59) mL · min-1 (1.73 m2)-1 vs.(101.09 ± 17.17) mL · min-1 · (1.73 m2)-1).The differences were statistically significant (t =7.621,13.591,15.126 and-5.120,all P ＜ 0.01).Compared with healthy control group,the renal injury indexes ACR and β2 microglobulin of simple obesity group were higher ((1.01 ± 0.32) μg/mg vs.(4.41 ± 1.16) μg/mg,(1.12 ± 0.15) mg/L vs.(1.46-± 0.26) mg/L),and the differences were statistically significant (t =9.732 and 7.792,both P ＜ 0.01).ACR of NAFLD patients was positively correlated with body mass index,waist circumference,triglyceride level,total cholesterol level,low density lipoprotein level,fasting blood glucose level,two-hour postprandial blood glucose,fasting insulin level and HOMA-IR (r =0.554,0.327,0.314,0.353,0.176,0.195,0.552,0.364 and 0.987,all P ＜ 0.05),and was negatively correlated with high density lipoprotein (r =-0.330,P ＜ 0.01).Conclusions NAFLD is closely related with chronic kidney disease at same baseline of gender,age,blood glucose,blood lipids,and blood pressure.Abnormal metabolism of glycolipids,obesity and insulin resistance may be the mechanisms of early kidney injury in NAFLD patients.Combination of ACR and β2 microglobulin are more sensitive than serum creatinine and eGFR in detecting early renal injury in NAFLD patients.

Objective:To evaluate angioimmunoblastic T-cell lymphoma(AITL) completely, we gave in-depth investigation of histopathological features, specific immunochemical markers, antigen receptor gene rearrangements and in situ hybridization for Epstein-Barr virus (EBV). Methods: 15 cases of typical AITL displayed effacement of the normal lymph node architecture partially or completely, abundance of arborizing high endothelial vessels, infiltration of polymorphic cells and hyperplastic atypical T lymphocytes with or without clear cytoplasm. Clinical characteristics, histological manifestations, and immunohistochemical staining for CD3, CD20, CD4,CD21, CXCL13, CD10, and BCL6 were analyzed. Polymerase chain reaction for immunoglobulin heavy chain (IgH) and T cell receptor ? (TCR?) rearrangements and in situ hybridization for Epstein-Barr virus encoded RNA (EBER-1) were performed.Results: Histologically, we found eight cases with regressed lymphoid follicles, six with absence of follicles and one with hyperplastic follicles with interfollicular lesions. We also found eight cases displaying aggregation of clear cells, four infiltration of large lymphoid cells, five abundant epithelioid histiocytes. CD20 staining showed hyperplasia of large B cells in four cases. CD21 expression exihibited extrafollicular expansion of follicular dendritic cell meshworks in 11 cases (73.3%), partially with a tendency of perivascular distribution. Positive rate for CXCL13 and CD10 are 73.3% and 6.7% respectively. Monoclonal rearrangements of TCR? were detected in 6/15 (40%) of cases, IgH rearrangements in 7/15 (46.7%), of which five were monoclonal, while two oligoclonal. 8 out of 15 cases (53.3%) contained EBV-positive cells. Among the four cases with large B cell proliferation, three were EBV-positive. Conclusion: AITL display great complexity and diversity clinicopathologically. Only when we recognize such diversity, can we reasonably apply and properly evaluate immunochemical markers and molecular techniques, and thus give a correct diagnosis.

Objective To investigate postoperative complications and proper treatment of craniopharyngioma after microsurgical resection. Methods The postoperative complications and treatments of 58 patients with craniopharyngioma who underwent microneurosurgical resection in our department were analyzed retrospectively. Results In the 58 cases ,45 cases received total resection, 11 cases received subtotal resection, and 2 cases received partial resection. Different levels of postoperative complications occurred in all of the cases, with diabetes insipidus in 55 cases , hypematremia and hyperchloraemia in 32 cases, hyponatremia in 25 cases, hyperpyrexia in 24 cases and hyperglycosemia acidosis in 2 cases. Forty-seven cases recovered clinically,9 cases improved,and 2 cases died. Conclusions Postoperative intensive monitor of water intake,and discharge,fluctuation of electrolyte, and actively correction of diabetes insipidus and electrolyte disturbances and maintaince of fluid balance may play an important role in postoperation treatment of craniopharyngiomas.

Little is known about whether tamoxifen (TAM) can affect resting state microglia apoptosis and about the cellular mechanism that may account for this. To explore this question, we incubated the microglia cell line BV-2 cells with TAM at different concentrations. Cell viability was assessed by the MTT assay, and flow cytometric analysis was performed to detect the cell apoptosis rate. Furthermore, mitochondrial membrane potential (Δψm) was tested by flow cytometry, and Bax, Bcl-2, Fas, and Fas-L expression was detected by Western blot. The results demonstrated that TAM decreased cell viability and induced apoptosis of BV-2 cells in a concentration- and time-dependent manner. In addition, disruption of Δψm was followed by up-regulated expression of pro-apoptotic Bax, Fas and Fas-L, and down-regulated expression of anti-apoptotic Bcl-2. These results indicate that TAM may induce apoptosis of BV-2 cells through both mitochondria- and death receptor-mediated pathways.

Objective To investigate the dyslipidemia in patients with psoriasis,and the characteristics of the serum lipids in pa-tients with different types of psoriasis.Methods 332 psoriatic patients were devided into pustular psoriatic,psoriasis vulgaris and erythrodermic psoriasis based on clinical manifestations.Serum lipids of the three groups were analyzed including TC,TG,HDL-C, LDL-C,ApoA1,ApoB and Lp(a).Results The serum concentrations of TC,HDL-C,ApoA1 in patients with psoriasis were signifi-cantly lower than those in control group(P <0.05),while the concentration of Lp(a)was significantly higher in psoriasis patients compared with those in control group(P <0.05).The serum level of TC,HDL-C,LDL-C and ApoA1 in pustular psoriasis group were significantly lower than those in the other two groups(P <0.05).Conclusion The patients with psoriasis have dyslipidemia, and the serious disorder situation could be associated with psoriasis types.