Objective To investigate the role of serum tissue polypeptide-specific antigen (TPS)detection in the evaluation of hepatocyte apoptosis in patients with alcoholic hepatitis and to further evaluate its application in the differential diagnosis of alcoholic hepatitis and fatty liver. Methods The automatic biochemistry analyzer was employed to detect common markers for liver injury (AST,ALT, GGT and bilirubin) ; electrochemiluminescence assay was performed to measure serum TPS level; immunohistochemistry staining was done to evaluate M30 immunoreactivity in hepatic tissue biopsy specimens and the apoptotic score was acquired. Then the correlations were analyzed between various indices and the apoptotic scores. Results The serum TPS level was positively correlative to the hepatocyte apoptotic score in alcoholic hepatitis group; and their correlation coefficient was the largest among various liver functional indices and the apoptotic scores. The correlation coefficient between TPS level and apoptotic score was significantly higher in alcoholic hepatitis group than that in fatty liver group (P＜0.05). Conclusion Serum TPS may be used as a good hepatocyte apoptosis marker for alcoholic hepatitis, which contributes to the differential diagnosis of alcoholic hepatitis and fatty liver.

Objective To explore the correlation between serum myocardial enzymes and children with viral hepatitis according to clinical observation and analysis,in order to guide clinical treatment.Methods 66 children with viral encephalitis admitted in Women and Children's Hospital of Ninbo City from September 2010 to December 2011 were used as observation group,the aspartate a minotransferase(AST)level,lactate dehydrogenase (LDH)level,creatine kinase levels(CK)level,MB isoenzyme of creatine kinase(CK-MB)level in serum were detected by Olym pus AU-1000 automatic biochemical analyzer.The level ofα-hydroxybutyric acid dehydrogenase(α-HBDH)was detected,too.At the same time,another 48 normal children in our hospital were used as control group,and the same indexes were detected and recorded.After obtain those datas in two groups,the differences of myocardial enzymes between observation group and control group,acute and convalescent,light and heavy encephalitis encephalitis, gammaglobulin gammaglobulin treatment and non-treatment group were compared and analyzed.Results showed that the degrees of myocardial damage were varied in children with viral inordinately,the more serious illness in children,the higher myocardial enzyme levels.Correspondingly,while the children with encephalitis were in recovery ,their cardiac enzyme levels were found significantly reduced.Adding gamma globulin may help cardiac enzymes decreasing at a faster rate,favoring patients' recovery.Conclusion Cardiac enzyme activity can be used as an important indicator of clinical observation in children with viral and their prognosis,proper treatment should be carried out when the relationship between serum myocardial enzymes and viral encephalitis are clear.

The aim of this study is to investigate comparatively the relationship between the endocrine and argyrophil cells in the rat gastrointestinal tract by means of the IGS, PAP immunohistochemical and Grimelius technique in the same sections. The results showed that the SS cells and almost G ceils can not be identified by Grimelius technique. The Grimelius technique had high specifity to reveal EC cells in the antrum of stomach and the intestine. Our technique is relatively simple and reliable, and may be used for consecutive demonstration of argyrophil cells and bioactive peptide-containing cells.

Acquired immune deficiency syndrome (AIDS), a great epidemic t hreatening disease, is caused by the human immunodeficiency virus (HIV), particu larly the strain known as HIV-1. Nevirapine, a novel non-nucleoside reverse tr anscriptase (RT) inhibitor combined with nucleoside analogue RT inhibitors or pr otease inhibitors, can be used in treatment of patients with HIV infection. AS a n agent used alone, nevirapine can prevent vertical transmission or HIV infectio n.

A novel fingerprint system of Chinese medicinal materials employing both DNA and chemical means has been proposed. To establish the fingerprint system is very effective to the quality control of Chinese medicinal materials, because it will be controlled from chemical substance and genetic information. The chemical components and DNA fingerprints will show the complex chemical components and biological source, respectively. DNA fingerprint could give a further proof to distinguish Chinese materia medica from the chemical. The combination of chemical and DNA fingerprints is constructive to quality control of Chinese materia medica, the cultivar identification and the selective breed of good varieties for medicinal use. To carry out the fingerprint system of Chinese medicinal materials, it is necessary to establish fingerprint database of Chinese medicinal materials, including chemical and DNA fingerprint, as well as many detection methods, and the pattern recognition system by computer is also necessary.

Objective To develop a HPLC method for the determination of protopine in Rhizoma Corydalis Decumbentis Capsules. Methods A HPLC was performed on a Hypersil ODS column( 4.6? 250 mm, 5 ? m) . The mobile phase was methanol - 0.1 % water solution of triethylamine (65∶ 35). Determination wavelength was 290 nm and flow rate was 1.0 mL/min. The theoretical plates should over 4000 according to the content of protopine. Results A good linearity was shown in the range of 0.3168～ 1.5840 ? g, r=0.9996. The average recovery was 100.6 % , RSD=2.68 % . Conclusion The method is simple, effective and can be used for the quality control of Rhizoma Corydalis Decumbentis Capsules.

Objective To develop a HPLC method for the determination of ephedrine hydrochloride in Xiaochuanning capsules.Methods A Hypersil ODS column was used.The mobile phase was acetonitrile-water solution of 0.05 mol / L NaH2PO4(pH2.7,22 ∶ 78).The flow rate was 1.0 mL / min and column temperature was 40 ℃.Detcted wavelength was 205 nm.Results A good linearity was in the range of 0.1005 ~ 0.5025 ?g(r = 0.9993).The average recovery was 103.1 %,RSD=1.81 %.Conclusion The method is simple,accurate,and can be used for the quality control of Xiaochuanning capsules.

AIM: To establish a HPLC method for determining of ginsenoside Re,ginsenoside Rg_1 and schisandrin in Huoliyuan Tablets(total ginsenoside,Radix Astragali,Fructus Schisandrae Chinensis,Radix Ophiopogonis,etc.). METHODS: A Hypersil C_(18) column was used.The mobile phase for ginsenoside Re and Rg_1 was acetonitrile-water mixed with 0.05 mol/L NaH_2PO_4(pH2.7,20∶80),and determination wavelength was 203 nm.The mobile phase for schisandrin was acetonitrile-0.1%H_3PO_4(48∶52),and determination wavelength was 250 nm. RESULTS: The linear ranges of ginsenoside Re,Rg_1 and schisandrin were 1.84-14.72 ?g(r=0.999 4),1.49-11.92 ?g(r=0.999 0) and 0.094-0.47 ?g(r=0.999 9),respectively.The average recoveries of ginsenoside Re,Rg_1 and schisandrin were 99.86%(RSD=1.69%),98.17%(RSD=2.03%) and 101.54%(RSD=(2.62%),) respectively. CONCLUSION: The method is simple,accurate,and can be used for the quality control of Huoliyuan Tablets.

We investigated the state of contemporary interpersonal relationships among postgraduates by the self-designed questionaires and then deeply discussed theirfeatures and put forward relative suggestions and strategies.

TLC scanning technique was used for the determination of tanshinone ⅡA in "Prostato Healthcare Bag" at ?S=470nm, and ?R = 620nm. This method is simple and highly sensitive, with an average recovery of 97.45％ and a variation coeffioient of 1,28%.