Objective To explore the value of the combined detection of serum CEA ,CA199 and CRP in the diagnosis and prog‐nosis of colorectal cancer .Methods Totally 150 patients with colorectal cancer treated in our hospital during 2012‐2014 were se‐lected as the observation subjects ,including 97 cases of histopathologically diagnosed of colorectal cancer without metastasis verified by the assisted examinations as the non‐metastatic group and 53 cases of metastasis verified by the assisted examinations as the me‐tastatic group ;at the sametime ,80 individuals undergoing the physical examination were taken as the control group .Then the levels of serum CEA ,CA199 and whole blood CRP level before surgery and after operation combined with 4 courses of chemotherapy or radiotherapy were compared between the observation group and the control group ,and between the non‐metastatic group and meta‐static group .Results The levels of serum CEA ,CA199 and whole blood CRP before treatment in the observation group were sig‐nificantly higher than those in the control group ,there was statistically significant difference between two groups (P<0 .01) ,more‐over the level of each indicator in the metastatic group of the observation group was higher than that in the non‐metastatic group , the difference between two groups was statistically significant (P<0 .01);compared before treatment ,the levels of serum CEA , CA199 and whole blood CRP after treatment in the observation group were decreased ,the differences between two groups were sta‐tistically significant (P<0 .01);moreover the sensitivity of the combined detection of CEA ,CA199 ,CRP was significantly higher than that of single indicator detection ,the difference was statistically significant (P<0 .01) .Conclusion The combined detection of serum CEA ,CA199 and whole blood CRP is of great importance for the diagnosis and prognosis of colorectal cancer ,which is con‐ducive to the early diagnosis and prognosis evaluation of colorectal cancer .

Objective To iavestigate the influence of two disinfectants methods on disintection efficiency and model precision of dental impression.Methods Alginate impressions,alginate and agar complex impressions and silicon rubber impressions were made according to a unified standard.Chlorinous disinfectant solution and acidic oxidative potential water were used to disinfect the infected impressions.The influence of two disinfectants on disinfection efficiency and model precision of dental impression was compared.Results Two disinfectants for 30 minutes had significant disinfection effect on these three kinds of impressions.The effective rate reached 99.48％.Chlorinous disinfectant solution bad no significant influence on the model precision of alginate impressions and silicon rubber impressions,but had significant influence on the model precision of alginate and agar complex impressions.Acidic oxidative potential water did not influence the model precision of the three dental impressions.Conclusions Acidic oxidative potential water can be used to disinfect the three dental impressions.Chlorinous disinfectant solution can be used to disinfect alginate impressions and silicon rubber impressions,but cannot be used to disinfect agar complex impressions.

Objective To study the chemical constituents of Paeonia veitchii. Methods Isolation and purification were carried out on repeated silica gel column chromatography. The structures of the compounds were identified by physico-chemical properties and spectral analyses. Results Nine compounds were isolated and identified as paeoniflorin (Ⅰ), hydroxypaeoniflorin (Ⅱ), benzoylpaeoniflorin (Ⅲ), benzoylhydroxypaeoniflorin (Ⅳ), albiflorin (Ⅴ), paeonisothujone (Ⅵ), mudanpinoic acid A (Ⅶ), 2-hydroxybenzyl alcohol (Ⅷ), bis (2-hydroxybenzyl) ether (Ⅸ). Conclusion Compound Ⅸ is obtained from natural products for the first time. Compounds Ⅳ, Ⅵ-Ⅸ are isolated from this plant for the first time.

Objective To summarize the experience of seven-step nursing method used in the care to patients with spontaneous esophageal rupture.Method Twenty patients with spontaneous esophageal rupture were treated with the seven-step nursing method. Results All patients were discharged after cure.The follow-ups found no complications of esophageal stenosis,reflux esophagitis and chronic empyema.Conclusion The seven-step nursing method used for caring the patients with spontaneous esophageal rupture can promote the recovery of patients and reduce their suffering.

Objective To analyze X-ray,CT and MRI characteristics of chondroblastoma.Methods Imaging(CT,X-ray and MRI)signs of chondroblastoma confirmed by pathology in 22 cases were analyzed.2 cases and 7 cases underwent contrast-enhanced CT and MRI examination respectivety.Results The lesions were located in tibia(n=11),femur(n=4),patella(n=2),humerus(n=1),temporal(n=1),mandibal(n=1),iliac bone with sacrum(n=1).X-ray manifestations were bone destruction(21),stripe or spotty calcification(5)and patchy sclerosis around the lesion(10).On CT,the lesions were lobular-and-oval(14),density of soft tissue(7)with stripe or spotty calcification(15),patchy sclerosis around(15)and soft tissue swelling(18).Mixed signal intensity on T2WI and FS T2WI,patchy long T1 and long T2 signal intensity around the lesions(20).Conclusion The imaging features of benign chondroblastoma are of certain characteristic and most signs on X-ray,CT and MR are corresponded with each other.

Objective To investigate the cognition and participation on organ donation in ICU medical staff and analyze the influencing factors, thus to provide reference for the development organ donation promotion measures. Methods 265 ICU medical staff were chosen by convenient sampling method from 10 three-level hospitals in Chongqing and were investigated with a self-designed questionnaire about the cognition, willingness and its influencing factors towards organ donation. Results ICU medical staff′s understanding of brain death, significance, basic conditions and concepts of organ donation were 67.2% (178/265), 61.1% (162/265), 60.0% (159/265), 55.8% (148/265) respectively, but understanding of Principles , registration methods, laws and regulations, and procedures of organ donation were 24.5%(65/265), 12.1%(32/265), 7.9%(21/265), 4.5%(12/265) respectively. 47.5%(126/265) ICU medical staff were willing to donate their own organs, 35.8%(95/265) were consented to relative′s organ donation and 4.5% (12/265) were organ donation applications. 89.1% (236/265) were affected by traditional ethical concepts, 70.2%(186/265) were for the publicity not enough, 51.7%(137/265) did not know about the procedures of organ donation, 37.0%(98/265) were for too much troubled procedures, and 43.8%(116/265) were for the ineffective work of relevant organizations. Conclusions Lacking in-depth understanding of organ donation and low willingness to donate organs in ICU medical staff in Chongqing. Mainly associated with the effect of traditional concept and lack of propaganda to the donation.

Objective To observe the effect of motivation of endothelial progenitor cells (EPCs) by granulocyte colony-stimulating factor (G-CSF) on cardiac function in patients with myocardial infarction (MI). Methods Eighty MI patients were sellectedand then were divided into a conventional treatment group (n = 40) and a EPC motivation group (n = 40). EPCs were detected by flow cytometry. Results The rate of EPCs was increased from (0.11 ± 0.04)% in the baseline to (5.32 ± 1.06)% (P < 0.05). Before treatment, 6MWT, LVEF, and LVEDD did not differ significantly between the conventional treatment group and the EPC motivation group (P > 0.05); after treatment, 6MWT, LVEF, and LVEDD were significantly increased in both groups (P < 0.05 and P < 0.01). However, 6MWT, LVEF, and LVEDD were higher in the EPC motivation group than in the conventional treatment group (P <0.05). The clinical effectiveness rate was higher in the EPC motivation group than in the conventional treatment group (90.0% vs. 75.0%, P < 0.05). There was no significant difference between the two groups in adverse reactions (7.5%vs. 10.0%, P > 0.05). Conclusions G-CSF can markedly motivate EPCs, which is beneficial for improvement of cardiac function in MI patients.

Objective To investigate the effect of gene therapy which was mediated by electroporation on the expression of basic fibroblast growth factor (bFGF) duning mandible distraction.Methods Bilateral mandibular osteotomies were performed in New-Zealand rabbit.After a latency of 3 days,the mandibles were elongated using distractors with a rate of 0.8 mm/d for 7 days.The rabbits were randomly divided into 5 groups:2 μtg recombinant plasmids pIRES-hVEGF165-hBMP2,pIRES-hBMP2,pIRES-hVEGF165,pIRES and normal saline (NS) were injected into the distraction area of groups A,B,C,D and E,after completion of distraction,respectively.The lengthened mandibles were harvested and processed for immunohistochemical detection of bFGF,and the mean optic densities and integral optical density of bFGF positive cells were measured by computerized image analyzer.Results bFGF mainly located in fibroblasts,giant monocytes,polynuclear phagocytes,osetocytes,and osetoblasts in the connective tissue around bone tissue.The strongest expression was observed at the 7th day,and weakened at 14th day of consolidation stage,there were no significant difference among groups A,B and C,at the 7th day of consolidation.However,there were significant differences between gene therapy groups (A,B and C) and control groups (D and E) (P＜0.01).Conclusions Gene therapy can enhance and prolong the expression of bFGF in distraction gap,which promotes the cell differentiation and proliferation,extracellular matrix synthesis and new bone formation during distraction osteogenesis.This is probably one of the molecular mechanisms of the gene therapy promoting new bone formation in distraction gap.

Objective To investigate the expression patterns of major bone growth factors and bone morphogenetic protein-2 (BMP2) in the distracted calluses following transfected gene during mandibular distraction osteogenesis in a rabbit model.Methods Bilateral mandibular osteotomies were performed in New-Zeland rabbits.After a latency of 3 days,the mandibles were elongated using distractors for 7 days.After the completion of distraction,the rabbits were randomly divided into 5 groups.Three animals each were sacrificed at the end of the delay phase,at 7,14,and 28 days after completion of distraction,respectively.The lengthened mandibles were harvested and processed for immunohistochemical detection of BMP2 expression,the mean optic densities and integral optical density of BMP2 positive cells were measured by computerized image analyzer.Results Elevated cellular expression of BMP2,in the distraction gap,was observed following mandibular distraction.BMP2 staining was mainly located in inflammatory cells,and the connective tissues arrounding the new bone.Their strongest expression was the 7th day,some of those growth factors expressed weakly or negatively.Conclusions Electroporation-mediated gene transfection can promote BMP2 expression effectively,which plays an important role in cell differentiation and proliferation during distraction osteo genesis.The BMP2 stimulates extracellular matrix synthesis,induces the proliferation and differentiation of fibroblasts and osteoblasts,which then promotes the new bone formation and repair.

OBJECTIVETo investgate the expression patterns of bone morphogenetic protein-2 (BMP-2) in the distraction area following plRES-hBMP2-VEGFI65 gene transfection at different time during mandibular distraction osteogenesis in a rabbit model.

METHODS48 New-Zeland rabbits were employed to underwent osteotomy and distraction devices implantation on mandible bilaterly. After 3 days of latency period, the devices were activated at the rate of 0. 8 mm per day for 10 days. The rabbits were randomly divided into 4 groups (group A, B, C and D). Group A, B and C were transfected recombinant plasmids pIRES-hBMP2-hVEGF165 via electroporation-mediated approach at latency period, distraction period, consolidation period respectively. Group D was used as control group without gene transfection. Three rabbits in each group were sacrificed at 1, 2, 4 weeks of consolidation respectively. The mandibles were harvested for immunohistochemical staining detection of BMP-2 expression respectively, which were analyzed by CMIAS series multifunction color quantitative analysis of pathological image analysis system.

RESULTSBMP-2 expression was found to be mainly located in the monocyte, fibroblast of the granulation tissue, the osteoblasts, osteocyte on the surface of new formed trabecular, and the connective tissues surrounding the new formed bone. The expression in group B was superior to other groups. Image analysis showed that, at the first week and second week of consolidation, the expression abosordbance A in group B (0. 58 ± 0. 03 and 0. 34 ± 0. 02) was relatively higher, when compared with that in group A (0. 42 ± 0. 02 and. 31 ±0.01), C(0.32 ±0.01 and 0.30 ±0.01)and D(0.27 ±0.01 and 0.23 ±0.02), showing a significant difference(P <0. 05). It was also relatively higher in group A(0. 42 ± 0. 02 and 0. 31 ± 0. 01) and C(0. 32 ± 0.01 and 0.30 ± 0.01), when compared with that in group D(0. 27 ±0.01 and 0.23 ± 0. 02), showing a significant difference( P < 0. 05) , but there was no significant difference ( P > 0. 05) between group A and group C. At the fourth week of consolidation, the expression decreased and there was no significant difference among group A, B, C, D.

CONCLUSIONSThe electroporation-mediated gene transfection which is transfected at the beginning of traction can promote BMP-2 expression effectively, stimulate bone marrix synthesis and induce proliferation and differentiation of fibroblasts, osteoblasts, endothelial cells, which further effectively promote the new bone formation. It suggests that the distraction stage is the optimal time for gene therapy.

Animals ; Bone Morphogenetic Protein 2 ; metabolism ; Electroporation ; methods ; Genetic Therapy ; methods ; Mandible ; surgery ; Osteogenesis ; Osteogenesis, Distraction ; Osteotomy ; Rabbits ; Time Factors ; Transfection ; methods