ObjectiveTo investigate the effect of early electro-acupuncture treatment on prevention of post-operative adhesions.Methods40 rabbits were randomly divided into 5 groups: normal control group, model group 1, model group 2, early electro-acupuncture group and later electro-acupuncture group with 8 animals in each group. Muscle adhesion model was established by operation. The early electro-acupuncture group was received the treatment from the 11th to 20th day after the operation; later electro-acupuncture group was from the 21st to 30th day after the operation. The weight, gait and range of motion (ROM) of animals were observed and analyzed.ResultsThe standing, walking and ROM of animals of the early electro-acupuncture group improved; but only few gait indexes improved and ROM not changed in the later electro-acupuncture group.ConclusionThe early electro-acupuncture treatment can prevent the post-operative adhesion of muscles, but not having effect on formed adhesion.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Face ; Female ; Humans ; Injections ; Melanosis ; drug therapy ; therapy ; Middle Aged ; Young Adult

Wide- angle viewing system as an important auxiliary device can clearly observe the whole fundus field of vision in vitreous surgery, which enable vitreoretinal surgery more efficient, safer and more effective. So it has very high application value in ophthalmologic operation. In this paper, we studied the development and application of wide - angle viewing system in vitreoretinal surgery in recent years, from which we summed up the advantage of non - contact wide - angle viewing system in clinical field, and pointed out the shortcomings. The ultimate goal is to make the non -contact wide - angle viewing system better applied in vitreous surgery.

Objective To analyse a novel splice mutation in EXT1 gene of hereditary multiple osteochondroma, and study its pathogenic mechanism.Methods In April of 2013, the proband was hospitalized from the outpatient department with multiple joint deformity for more than 20 years, peripheral blood of the proband and his parents were collected and genomic DNA was extracted .Coding regions and adjacent intron sequences of EXT1/EXT2 genes in genomic DNA of the family members were amplified and sequenced.Bioinformatics was used to analyze the mutation from sequencing .cDNA from peripheral blood of the proband ,the mother and normal control was made respectively as a template for amplifying coding regions of EXT1 gene, and the product was T-A cloned and sequenced.The abnormal transcripts of each group were counted and analyzed using chi square test to study the pathogenic mechanism of the mutation .Results Sequencing results of family members revealed that there was a heterozygous deletion mutation ( c.1284 +2del) in the 5′splice site of intron 4 in EXT1 gene of the proband and his mother .Bioinformatics predicted that exon 4 of EXT1 gene was skipping or spliced aberrantly due to the mutation .T-A clone and sequencing results as well as the statistical analysis suggested that there was a significantly higher proportion of transcripts with skipping exon 4 in the proband and his mother compared with the normal control (P=0.000, P<0.01).Conclusions c.1284+2del in EXT1 gene is reported for the first time internationally , which results in a considerable number of abnormal transcripts with skipping exon 4 in EXT1 gene, thereby influences the normal transcription and translation of EXT1 gene.

Objective To explore the experience of nursing patients with Crohn’s disease.Method The nursing histories of 80 patients with Crohn’s disease were retrospectively reviewed to analyze and summarize the measures for nursing them.Result After active treatment and effective nursing,all the patients were improved and discharged.Conclusion Effective nursing is of positive significance to improve the curative effect of Crohn's disease.

Objective To analyze the characteristics of hospitalized patients with systemic lupus erythematosus (SLE) in Ningxia Hui Autonomous Region,and to characterize the epidemiology of SLE.Methods The data of 184 patients admitted to Ningxia Medical College Hospital during January 2008 to December 2013 were retrospectively analyzed.The data analysis included the initial symptoms,system involvement and clinical situations.Chi-square test,t test,Mann-Whitney U test and Logistic analysis were used for statistical analysis.Results ① The ratio of male and female was 1∶9.8.The most common clinical symptoms was rash in 72 cases (39％),and followed by joint pain in 61 cases (33％),swelling in 28 cases (15％) and fatigue in 21 cases (11％).② Eighteen percent (33/184) patients were late-onset SLE patients.Compared with regular SLE patients,the interval was longer in late-onset SLE patients between clinical symptoms onset and the time when they saw the doctor.③ Blood and kidney were the most commonly affected organs.④ The highest diagnosis rate occurred in rheumatology department,the highest diagnosis rate occurred in the kidney department for patients with dropsy as starting symptom.Conclusion SLE primarily occurs in childbearing aged women.The reason of misdiagnosis of SLE is mainly due to the incorrect hospital selection for the first visit.Delayed diagnosis will lead to delay in the treatment.

Objective To observe the bioactivity of recombinant human scFv antibody against NH-lipopolysaccharide binding protein in vitro and in vivo. Methods KM mice, weighing 20-25 g, were burnt on the back to the third degree covering 20% total body surface area. The mice in only burn group were intraperitoneally injected with 1 ?g/g LPS and those in scFv-treated group with 1 ?g/g LPS and 0.5 ?g/g scFv after burn. The mice without any treatment served as controls. All mice were sacrificed in 1, 3, 6, 12, 24 h after all treatment and the whole blood, the tissues of liver, lung and kidney were collected. The serum concentration of TNF-?, IL-6 in burnt mice with endotoxemia was detected by ELISA and the pathological changes of liver, lung and kidney tissues were observed by light microscope. The inhibition of scFv antibody of different concentrations against NH-lipopolysaccharide binding protein on FITC-LPS binding with U937 cells was assayed by FCM. Results The histopathological changes of liver, lung and kidney in mice of scFv-treated group revealed that less inflammatory cell infiltration, less degenerated cells, weaker congestion in tissue as compared with control and only burnt mice. The serum concentration of TNF-? in only burnt mice increased significantly than that of the controls (P

AIM: To investigate the potential role of 14-3-3 tau in trophoblast cells on invasiveness. METHODS: 14-3-3 tau expression was detected in first-trimester villi, deciduas and human trophoblastic cell line (BeWo) by immunohistochemistry. Small interference RNA (siRNA) targeting 14-3-3 tau was transfected into BeWo cells. The effects of down-regulated 14-3-3 tau on invasion of human trophoblasts cell line BeWo were examined by matrigel invasion assay, and the transcription, translation of E-cadherin and snail were estimated by RT-PCR or Western blotting. U0126 was used to detect the extracellular-signal related kinase 1/2 (ERK1/2) function on down-regulation of 14-3-3 tau induced cell invasion. RESULTS: 14-3-3 tau was detected in the invasive trophoblastic cells in the first trimester villi and that invaded to the deciduas. BeWo cells also expressed 14-3-3 tau. Down-regulation of 14-3-3 tau increased the invasive cell-number of BeWo, as well as the expression of snail, and inhibited E-cadherin. U0126 inhibited the enhanced invasiveness in these cells induced by the down-regulation of 14-3-3 tau. CONCLUSION: 14-3-3 tau may regulate the invasiveness of human trophoblastic cells through ERK1/2 signaling pathway.

Objective To analyze the distribution and drug resistance rate of methicillin-resisitant Staphylococcus aureus(MRSA) and methicillin-susceptible Staphylococcus aureus(MSSA) isolated from different departments in a certain primary hospital during 2009 to 2012,and to provide scientific evidences for clinical application of antibiotics.Methods Pathogens and bacterial resistance to antibiotics were identified using the VITEK 2 compact equipment.The data were got from WHONTES.5 and analyzed by SPSS 13.0.Results There were 517 Staphylococcus aureus strains were detected(MRSA 135 strains,MSSA 382 strains).The rate of MRSA was 24.5％,27.7％,24.8％,27.0％ during the four years.MRSA was mainly found in the department of oncology,orthopaedicsand ICU.MRSA was mainly isolated from pus,secretion,sputum and blood.The 517 Staphylococcus aureus strains showed high sensitive to vancomycin,linezolid and teicoplanin,the sensitive rate was 100％.Conclusion Establishing a more comprehensive MDRO monitoring and hospital infection control system in the primary hospital,and rational using antibacterial drugs at the based of the antibiotics susceptibility test in the treatment can be effective in preventing MRSA resistance rates increasing and hospital-borne.

Carbon Monoxide ; physiology ; Humans ; Nervous System Diseases ; physiopathology ; Neurotransmitter Agents ; physiology ; Nitric Oxide ; physiology