BACKGROUND: Islet transplantation is an effective method for the treatment of type 1 diabetes mellitus and parts of type 2 diabetes mellitus. However, its application is hindered by insufficient sources and immunologic rejection. Though transdifferentiation of pancreatic stem cells is at the starting step, it is thought to be the hopeful source for islet cell transplantation.OBJECTIVE: To look for a suitable cells-transplantation source for the treatment of diabetes mellitus. METHODS: The pancreatic ductal epithelial cells were separated from Kunming mice and cultured in DMEM/F12 medium supplemented with keratinocyte growth factor, hepatocyte growth factor and nicotinamide, etc. Samples were taken at different time points for light microscopy and electron microscope. The changes of CK-19 and PDX-1 were detected by immunocytochemistry at 1 and 16 days. The expressions of insulin and glucagon gene were detected by RT-PCR at 1 and 16 days. The physiologic function of these islet-like clusters was determined by dithizone staining and glucose stimulation at 21 days. RESULTS AND CONCLUSION: A large number of epitheliod cells were CK-19 immunoreactive positive and few of them were PDX-1 positive at 1 day after isolation, then CK-19 positive cells proliferated quickly and formed substantial plaques of epithelial cells in cobblestone pattern. At 16 days later, these cells begin to form islet-like clusters gradually, while most of them were PDX-1 immunoreactive positive. The analysis of mRNA by RT-PCR showed very low levels of insulin and glucagon mRNA in the starting materials but increase was found as the process of transdifferentiation. At 21 day differentiated islet-like clusters were stained red by dithizone. In those samples exposed to a stimulatory 15 mmol/L glucose, there was a 1.6-fold increase in insulin compared with to 5.6 mmol/L glucose (P < 0.05). Pancreatic ductal cells of adult Kunming mice could proliferate quickly and have the potency of transdifferentiation into islet-like clusters when cultured in vitro under appropriate conditions.

Objective　To explore the method of chemical synth esis of L-alanyl-L-glutamine dipeptide. Methods　 L-alanyl-L -glutamine dipeptide was prepared by condensation and ammoniation reaction of L -glutamine and 2-D-chloropropionyl chloride. The physico-chemical properties of this dipeptide were identified with melting point determination, amino acid analysis, elements analysis and MS and its purity was determined with HPLC. Results　This dipeptide was confirmed good in quality by physico-chem ical determination. Conclusion　Dipeptide synthetized by this me thod is simple, convenient and with good guality.

Objective To investigate the oxidative DNA damage in lymphocyte of MHD patients and the effect of dialysis membrane on it through comet assay.Methods From 2003-05 to 2004-10 we selected 20 CRF patients undergoing maintenance hemodialysis and divided the patients into HE and PS group randomly.And 7 volunteers were chosen as controls.The patients in each group had undergone hemodialysis for 12 weeks using HE or PS membrane respectively.Then we took venous blood sample and separated lymphocyte.The oxidative DNA damage was measured by comet assay.Results The comet tail length of control group,HE group and PS group was(0.286?0.194)?m,(0.970?0.120)?m and(0.656?0.187)?m respectively.Compared with control group,the comet tail length of MHD patients was significantly longer(P

Objective: To investigate the protective effect of trimetazidine on experimental myocardial ischemia and its mechanism. Methods: Fifty mice received isoproterenol (20 mg?kg -1 ?2 d,ip) were divided into control and treatment groups. The myocyte ultrastructure,serum creatine phosphokinase (CK) activities and lactate dehydrogenase (LDH), myocardial malondialdehyde (MDA) and superoxide dismutase (SOD) were observed in 3 groups. Results: Compared with the controls,in 2 groups pretreated with trimetazidine (5 mg?kg -1 ?d -1 and 10 mg?kg -1 ?d -1 ? 7 d, ip), the degree of myocardial damage were significantly reduced;the serum CK and LDH were lower;the myocardial MDA was lower;the myocardial SOD was higher. Conclusion: Trimetazidine can significantly reduce the degree of myocardial damage produced by isoproterenol;and it may play an important role in protecting ischemic myocardium, the mechanism may be associated with reduced oxygen free radical production.

0 05) CONCLUSION:The results of the statistical analysis showed that the two formulations were bioequivalent

Objective To isolate and purify polysaccharide from Angelica sinensis by ultrafiltration technology. Methods Extract of polysaccharide of Angelica sinensis was ultrafiltrated with different aperture membrane, the separation parameters on membrane separation efficiency of polysaccharide of Angelica sinensis were optimized by and orthogonal test. Results Polysaccharide of Angelica sinensis was separated with 200, 100, 50, 20 kD membrane. The contents of polysaccharide was 30.47% before ultrafiltration. The main component of Angelica sinensis polysaccharide was the fraction with 200 kD (65.42%). The optimum condition of ultrafiltration was obtained as follow:one times of extract volume, 35 ℃, 0.3 MPa. Conclusion The technology was simple and available, and the content of Angelica sinensis polysaccharide was higher.

Liver fibrosis is a pathological process for the liver to repair various chronic liver injuries and may eventually progress to liver cirrhosis,liver failure,or even liver cancer.Therefore,early intervention arid treatment of liver fibrosis is of particular importance.This article summarizes the latest research advances in the roles of TGF-β/Smad signaling pathway,Notch signaling pathway,Wnt signaling pathway,Hedgehog signaling pathway,and integrin signaling pathway in the pathogenesis of liver fibrosis and points out that these signaling pathways play an extremely important role in the development and progression of liver fibrosis and may provide new directions for the treatment of liver fibrosis.

Objective To investigate the relationship among CEA, CA199, clinical pathological characteristics and prognosis in patients with rectal cancer. Methods A total of 142 patients diagnosed rectal cancer were collected for the study between January 2006 and December 2010. The clinical data including age, sex, size of tumor, differentiation, depth of tumor (T), lymph node metastasis (N), distant metastasis (M), lymphatic invasion, venous invasion, stage, and preoperative serum levels of CEA and CA199 were obtained. Patients′ follow-up ranged from 42 months to 90 months. Results Preoperative elevated CEA levels were significantly associated with distant metastasis (P < 0.05). Median overall survival time was 150 weeks in the group with normal preoperative CEA and CA199, and 101 weeks in the group with preoperative abnormal preoperative CEA and/or CA199, and Kaplan-Meier analysis showed that the difference was significant between two groups (P = 0.031). Conclusion Elevated preoperative CEA level can predict distant metastasis. The patients with abnormal preoperative CEA and/or CA199 have shorter overall survival time.

Obejective To explore the correlation of Orexin A and respiratory drive in chronic obstructive pulmo-nary disease (COPD) patients. Methods Pulmonary function was tested in 30 stable COPD patients and 20 normal healthy adults. Plasma orexin A level was measured with a radioimmunoassay kit. The correlation of Orexin A with BMI, age, BDI, FEV1, FVC, FEV1/FVC, MEP, MIP, P0.1 in COPD patients was analyzed. Results Plasma Orexin A levels in patients with COPD group(1.87±0.43)ng/L was higher than those in the control group(1.49±0.19)ng/L, P<0.01. Plasma Orexin A lev-els in patients with COPD correlated negatively with FEV1(r=-0.389,P < 0.05),and correlated positively with P0.1(r=0.728,P<0.01). Conclusion Plasma orexin A in COPD patients increased which may be caused by smoking and hyper-capnia. Orexin A may play a crucial role in regulating respiratory drive in COPD patients.

Objective To study on the effect of dietary nutrition intervention in community on nutrition knowledge and dietary compliance in patients with psoriasis.Methods 40 patients with psoriasis were given a balanced diet pagoda and food exchange method,along with psoriasis nutrition knowledge manual to make individual and concise diet prescription.The nutrition knowledge and dietary compliance of patients before and after the intervention were compared.Results In a nutrition knowledge test,before the intervention,31 patients obtained a score below 60 which accounted for 77.5％,and 9 patients got higher score which meant 22.5％ patients had a good dietary compliance.After the intervention,18 patients (45.0％) got a score below 60,while 22 (55.5％) patients got a good score,the results after the intervention were statistically higher than those before the intervention.Conclusions Patients with psoriasis have poor cognitions and bad dietary compliance.After dietary nutrition intervention,the nutrition knowledge is enhanced and the dietary compliance gets higher,the dietary structure is improved,and consequently accelerate the recovery of disease.