ObjectiveTo investigate the mechanism of TNF-? on regulating the blood-labyrinth barrier permeability of cochlea of guinea pig.MethodsThe stria vascularis was acquired from guinea pigs and the endothelial cells were cultured of with purified to establish the models of the blood-labyrinth barrier according to our previous researches.The models were treated with TNF-?(0.05,0.1,0.2 ng/ml)for 30,60,90 min or with serum-free DMEM as control.Then their permeability was measured by Evens blue technique.ResultsTNF-? increases Evans blue transfer across endothelial cell monolayer(P

Objective:To observe the effect of complex reinforcing [Shao Shan Huo (Mountain-burning Fire)] and reducing [Tou Tian Liang (Heaven-penetrating Cooling)] manipulations on motion and balance of the lower limbs in patients with subacute combined degeneration of the spinal cord (SCD). Methods:A total of 100 SCD cases were randomly allocated into an observation group and a control group by their visit sequence, 50 cases in each group. On the basis of Western medical treatment, cases in the observation group were also treated with acupuncture therapy plus complex reinforcing-reducing manipulation; whereas cases in the control group were only treated with the same Western medical treatment as those in the observation group. Before and 2 months after treatment, kinematic parameters including muscle force, muscle tone and range of motion of the hip and knee joints were measured. In addition, balancing parameters including the path length of center of pressure (COP), peripheral area, COP path length per unit area and rectangular area were also measured before and after treatment. Results:After treatment, there were statistical intra-group differences in COP path length, peripheral area, COP path length per unit area, left-right offset, rectangular area, coefficient of stability and coefficient of weight distribution (P<0.01). There were statistical inter-group differences in muscle force, muscle tone and range of motion of hip and knee joints (P<0.01). The total effective rate was 83.3% in the observation group, versus 60.0% in the control group, showing a statistical difference (P<0.05). Conclusion:Complex reinforcing-reducing manipulations can improve the lower limb motion and balance in SCD patients.

Objective To assess the effects of Mtwo for root canal therapy in the aged patients .Methods One hundred and fifty-two root canals in 64 teeth for root canal therapy (RCT) in aged patients(60~70years)were randomly divided into M groups and K groups.Mtwo machine with nickel titanium file was used in M group (32 teeth, 78 root canals), and stainless steel file in K group (32 teeth, 74 root canals).Root canal preparation time, root spread reaction, and canal filling effects were compared between two groups.Results The root canal preparation and filling in group M were better than in K group ( P <0.05).The time consumption of RCT in M group was shorter than in group K .Conclusions Mtwo shows good shaping ability and is safe for aged patients in root canal treatment with high efficiency .

Objective To explore the operation al skills of laparoscopic appendectomy(LA) and to improve its proficiency.Methods The clinical data of 121 patients under went LA during February 2013 to May 2014 were summarized retrospectively.Results All the 121 cases were completed successfully with no complication.Conclusion With the development of laparoscope and accumulation of experiences LA can be safe and complications can be minimized if the this operation skill being obtained.

BACKGROUND:Mesenchymal stem cels can differentiate into lung parenchymal cels involved in lung injury repair, providing a new approach for the application of mesenchymal stem cels in patients with chronic obstructive pulmonary disease. OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cel transplantation on the repair of airway injury in rats with chronic obstructive pulmonary disease. METHODS:Twenty-four female rats were randomized into four groups: bone marrow mesenchymal stem cel transplantation group (cel transplantation group,n=12); bone marrow mesenchymal stem cels group (cel control group,n=4); model group (n=4); healthy control group (n=4). Rat models of chronic obstructive pulmonary disease were established in the cel transplantation group and model group using fumigation+lipopolysaccharide method; and at 1 day after modeling, model rats were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cels and 1 mL PBSvia the tail vein in these two groups, respectively. In addition to tracheal injection of normal saline (300 μL) at 1 and 14 days, rats in the cel control and healthy control groups were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cels and 1 mL PBSvia the tail vein, respectively. At 1, 7, 15 and 30 days after cel transplantation, lung tissue and serum markers of al rats were detected. RESULTS AND CONCLUSION:(1) Hematoxylin-eosin staining showed that emphysema and airway injury was milder in the cel transplantation group than the model group, but severer than the cel control and healthy control groups. (2) The total number of leukocytes and neutrophils in the peripheral blood was higher in the cel transplantation group than the cel control and healthy control groups (P < 0.05); with time, the total number of leukocytes and neutrophils was decreased gradualy. (3) Compared with the cel control and healthy control groups, the interleukin-10 level in the peripheral blood was lower and the levels of tumor necrosis factor-α and granulocyte colony-stimulating factor were higher at 1 day after cel transplantation (P < 0.05). With time, in the cel transplantation group, the interleukin-10 level was increased gradualy, the level of tumor necrosis factor-α was decreased gradualy, and the level of granulocyte colony-stimulating factor was increased first and then decreased, which was highest at 7 days after cel transplantation. (4) Partial CM-Dil-positive cels were positive for CC16. Taken together, bone marrow mesenchymal stem cel transplantationvia the tail vein can improve lung injury of rats with chronic obstructive pulmonary disease, and it is involved in the repair of airway injury through differentiation into epithelial cels and immune regulation.

Objective To observe the changes of F-actin of strial capillary endothelial cells from guinea pig cochlea after TNF-? treatment so as to further study the mechanism of permeability of those cells.Methods Strial capillary endothelial cells were dissociated from guinea pig cochlea and cultured respectively with 0.05,0.1,0.2 ng/ml TNF-? for 90 min,then detected by immunofluorescence laser confocal microscopy for F-actin concentrations.The blank control was set simultaneously.Results TNF-? decreased the content of F-actin in strial capillary endothelial cells(P

ObjectiveTo observe the effect of pelvic exercise to improve walking ability in hemiplegia. Methods60 stroke patients were devided into 2 groups, pelvic exercise group( 30 cases) and control group(30 cases). The walking ability in all patients was evaluated to compare the effect between two groups.ResultsThe excellent and good rate was 93.3% in pelvic exercise group. As compared with control group, there is a significant improvement in walking ability in pelvic excise group. ConclusionsPelvic exercise can significantly improve walking ability in stroke patients.

BACKGROUND: SARS, as a kind of sudden stress source, make quite a few people present symptoms such as panic emotion, psychological allergic reaction, etc. There are also partial nurse students who worry about to be infected, fear to practice in clinical departments and live in an irritable mood, which interferes their clinical practice and affects both body and mind health.OBJECTIVE: Through the investigation of the nurse students' stress response and anxiety symptom to understand their psychological stress response scale and anxiety level during the prevailing period of SARS.DESIGN: An intersectional investigation with the nurse students doing clinical practice or probation apprentice as observative objects.SETTING: Department of Psychiatry, West China Hospital, Sichuan Univesity PARTICIPANTS: In May 2003, the nurse students of 2-4 grade doing clinical practice and probation in West China Nursing College of Sichuan University were selected as observative objects, all of whom had no record of mental illness or other severe disease, and volunteered to receive the investigation. 305 questionnaires were sent out and 299 valid questionnaires were taken back. They were 171 junior college students (47.2%) and 128undergraduates (42.8%) of 18-32 years old, average 21.51,and boy students held 4.6% while girl students 93.4%.METHODS: The crisis response questionnaire was used to investigate the nurse students' response scale to SARS crisis from three aspects such as cognition, emotion and behavior. The response grade is from 1 to 10.The total score scale is 3-30. 3 means no impairment, 3-9 means is a little impairment, 9-15 means slight impairment, 15-21 means moderate, 21-27 means significant, and more than 27 means severe. The self-rating anxiety scale (SAS) compiled by Zung in 1971 was mainly used to assess the frequency of the appearance of the symptoms defined in each item in the students being investigated, including 20 items and score in 4 grades: 1 indicates never (appear) or in a little time,2 indicates in a small part of time,3indicates in a large part of time,4 indicates almost in the whole or in the whole time. Only the matter of fact in the recent week was investigated in the above two questionnaires. The students were required to fill in the questionnaires separately on the spot, and the questionnaires were handed in as soon as they were filled out.MAIN OUTCOME MEASURES: The nurse students' response scale and anxiety level to SARS crisis on cognition, emotion and behavior.RESULTS: 305 questionnaires were sent out and 299 were taken back,with a back rate of 98%. ①Crisis response scale: in the three aspects of cognition, emotion and behavior of the nurse students, 21.7% of them had no impairment,56.8% had a little impairment, 13.8% had slight impairment,5.4% had moderate impairment ,1.7% had significant impairment and only 0.7% had severe impairment. The score of the undergraduates on their cognition, emotion and behavior and the total score of crisis response were obviously higher than those of the junior college students (P ＜ 0.05).②Anxiety level: the nurse students' self-assessed result was that the total score was higher than that of the average normal Chinese [(39.89±11.98,29.78±0.46),(P＜0.05)],the number of them with score higher than normal level made up 82.3% and there was no significant difference in scores on SAS between junior college students and undergraduates. ③Relation between crisis response and anxiety level: the analytic result of the relation between the stress response scale and anxiety level indicated that they had relativity (r=0.685 ,P ＜0.01).CONCLUSION: Facing the stress response to SARS crisis, the nurse students are impaired to some extent on their cognition, emotion and behavior.Their anxiety level are higher than that of the average normal Chinese and their psychological stress response scale has positive correlation with the anxiety level.

Air Pollutants ; analysis ; Dust ; analysis ; Environment Design ; Environmental Monitoring ; instrumentation