In the article the design and making method of improved chincap with Lengthwise hook and protraction appliances are introduced.The appliances are mainly used to treat early-stage skeletal crossbite of antCrior teeth,which can't be solved by simple intramaxillary anchorage appliances.The analysis of pre and post cephalometrics of 11 cases yields signifi- cant differences (P

Objective To investigate the expression of microRNA in plasma of gallbladder cancer and its clinical significance.Methods miRNA microarray was performed in seven gallbladder cancer tissues and seven adjacent normal gallbladder tissues to identify differentially expressed miRNA.The results were validated in plasmas of 40 gallbladder cancers and 20 health people with real-time polymerase chain reaction (RT-PCR).Results Eleven gallbladder cancer-related miRNAs were identified with miRNA microarray analysis.Among them,miRNA-21,miRNA-370,miRNA-187,miRNA-122,and miRNA-202 were up-regulated,and let-7a,miRNA-200b,miRNA-143,miRNA-31,miRNA-335,and miRNA-551 were down-regulated in gallbladder cancers.Moreover,let-7a,miRNA-21,miRNA-187,miRNA-143,miRNA-202,and miRNA-335 were validated with RT-PCR,which were consistent with miRNA microarray results with significant difference between gallbladder cancers and health people (P ＜ 0.05).Furthermore,miRNA-187,miRNA-143,and miRNA-202 were related to clinical and pathological features(P ＜0.05).Conclusions These data indicate that The differentially expressed plasma miRNAs are related to gallbladder carcinogenesis and might be the novel biomarkers of gallbladder cancers.

Objective To discuss value of transvaginal ultrasound combined with abdominal ultrasound in the diagnosis of gynecologic acute abdomen.Methods 102 cases with gynecologic acute abdomen who were treated in our department were selected,they got through transvaginal ultrasound combined with abdominal ultrasound.The diagnosis result and pathologic diagnosis,simple ultrasound diagnosis result were compared.Results Compared with transvaginal ultrasound,abdominal ultrasound diagnosis coincidence rate obviously decreased,the difference was statistically significant (t =5.17,P <0.05 ),ultrasound combination diagnosis coincidence rate all obviously increased,the difference was statistically significant (t =11.25,P <0.01 ).The highest ultrasound combination diagnosis coincidence rate was heterotopic pregnancy (100.00%),the lowest was perforation uterus (75.00%). Average ultrasound combination diagnosis coincidence rate in this research was 86.78%,misdiagnosis rate was 1.96%,and missed diagnosis rate was 3.92%.Conclusion Accuracy rate of transvaginal ultrasound combined with abdominal ultrasound pathologic diagnosis results,have these following advantages,such as high accuracy rate,rapid diagnosis,suitable for gynecologic acute abdomen diagnosis.

Neurodegenerative diseases are chronic lesions caused by neuronal degeneration and secondary demyelination.Recent studies have shown that a DNA/RNA binding protein named fused in sarcoma/translocated in liposarcoma protein (FUS/TLS) is associated with neurodegenerative diseases,such as amyotrophic lateral sclerosis (ALS),frontotemporal dementia (FTD) and polyglutamine diseases.Mutations or dysfunction in FUS/TLS are responsible for the abnormal processes in the related gene transcription,RNA processing,transportation and translation and are highly correlated with the development of neurodegenerative disease.Discovery and the research of FUS/TLS not only contribute to the understanding of neurodegenerative disease at RNA level,but also provide a new way for the prevention and treatment of neurodegenerative diseases.

Synergistic Innovation is the developing trend and requirements of the times and society.Southern Medical University practiced joint mode among colleges and universities for excellent medical talent cultivation.Based on preponderant discipline and taking mechanism innovation as the key points,Southern Medical University actively explored the diversification mode of synergistic innovation.

This article made primary reseach on the following aspects: fetus's legal status and civil rights,applied law of compensation for damages,legal responsibility' assumed,etc.

Acquired Hyperostosis Syndrome ; therapy ; Combined Modality Therapy ; Humans ; Integrative Medicine ; Male ; Middle Aged

To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and menstrual cycle, the regulatory effects of 17-beta-estradiol (E(2)) and medroxyprogesterone acetate (MPA) on the expression of COX-2 in cervical cancer Hela cells were examined. Cervical cancer specimens were obtained from 47 pre-menopausal patients. The phase of menstrual cycle was determined by case history and HE staining of uterine endometrium. COX-2 was immunohistochemically stained by SABC staining and the staining intensity was determined with computerized image analysis system. Hela cells were incubated with alcohol, E(2), E(2)+MPA, MPA for 12, 24 and 48 h respectively. The expression of COX-2 in Hela cells was detected by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Our results showed that the expression of COX-2 was significantly higher during proliferative phase than secretory phase (P<0.05), but there was no difference in the positive rate between proliferative phase and secretory phase (P>0.05). Incubation with E(2) could significantly enhance the expression of COX-2 continually. On the contrary, E(2)+MPA and MPA alone could decrease the expression of COX-2 as compared with the control and E(2) group (P<0.05 and P<0.01 respectively). It is concluded that the expression of COX-2 in cervical cancer of pre-menopausal patients and Hela cells was regulated by estrogen/progestogen.
Cyclooxygenase 2/genetics ; Cyclooxygenase 2/*metabolism ; Estradiol/*pharmacology ; Hela Cells ; Medroxyprogesterone Acetate/*pharmacology ; Uterine Cervical Neoplasms/*enzymology

Objective:To investigate the 256 slice spiral CT pulmonary angiography (CTPA) application value by using multiple stage double mixed injection velocity tracking method in the diagnosis of pulmonary embolism.Methods: Fifty patients with pulmonary embolism from 2014 January to 2015 January were treated in our hospital patients as the research object. Using a random number table method, all patients were randomly divided into observation group and control group, 25 cases each group of patients, two groups of patients in our hospital by imaging with 256 slice spiral CT pulmonary artery (CTPA), the control group were treated by routine pre injection time density curve method, was used in the observation group than in mixed injection agent tracking method by two physicians, CT, using the double-blind assessment of two groups of patients with image quality and quantification of pulmonary artery trunk.Results: in the control group, 25 patients were satisfied with the time - density curve, the results of 5 conventional image quality scores showed no significant difference between the two imaging methods, was not statistically significant; two groups of patients with pulmonary artery trunk, branch clear imaging score and endovascular CT value had no significant difference(t=1.892,t=1.952,t=1.832;P>0.05), but the observation group patients with CTPA were examined by contrast agent dose was significantly lower than the control group, the patient''s heart rate, blood pressure was significantly lower than the control group(t=2.315,t=1.958,t=2.982;P<0.05), with statistical significance. Conclusion: CTPA with multi period mixed double flow injection method can guarantee the tracking based on image quality and diagnostic value can effectively reduce the contrast agent dosage and application effects of cardiac function, and the examination is relatively simple, safe, has clinical value of application and popularization.

Objective To observe the efficacy of Er ∶ YAG laser and pulsed dye laser (PDL) in treating facial acne scars.Methods A total of 78 cases with facial acne scars were divided into two groups.The test group included 40 patients that were treated by Er ∶ YAG laser (total three treatments,once three months)and irradiated by PDL (total four times,once per month),while the control group included 38 patients treated by Er ∶ YAG laser only.After three months of the last treatment,the effective rate and side effects were recorded and compared.Results The efficacy of the test group reached 92.5％ (37/40) and 77.5％ (31/40) for acne scars and post-acne erythema,respectively.However,the effective rate of the control group was 73.7％ (28/38) and 47.4％ (18/38).There were statistically significant differences between two groups (P＜0.05).Regarding reported adverse effects,there were four cases in the test group and two cases in the control group that appeared mild pigmentation.After symptomatic treatment,the skin color recovered.There was no other side effect in both two groups.Conclusions The effectiveness of Er ∶ YAG laser and PDL is better than Er ∶ YAG laser only in treating facial acne scars,and thus it is worthwhile for popularization and application in acne scars.