Antioxidants ; metabolism ; therapeutic use ; Bronchopulmonary Dysplasia ; drug therapy ; etiology ; metabolism ; Humans ; Hyperoxia ; complications ; Infant, Newborn ; Lung ; metabolism ; Reactive Oxygen Species ; metabolism

ObjectiveTo obtain the soluble expression of P protein of norovirus(NV)G Ⅱ. 2[P16]and analyze its antigenic characteristics.MethodsThe recombinant plasmid GⅡ.2-NV-pET-28a was constructed by optimizing the NV GⅡ.2[P16]P protein gene,synthesizing and cloning it into pET-28a(+)vector. The recombinant plasmid was transformed into DH5α competent cells,and the expression strain was optimized to make the P protein express in soluble form. The expressed P protein was purified by Ni-NTA affinity chromatography,of which the antigenicity was detected by Western blot,the purity was detected by size exclusion high performance liquid chromatography(SEC-HPLC),and the antigenantibody binding ability was identified by ELISA.ResultsThe target protein of recombinant BL21 Star(DE3)pLySs was highly expressed in soluble form. After purification by affinity chromatography,the purity of GⅡ.2 P protein was 95. 53%,and the antigenicity was good.ConclusionSoluble NV P protein was successfully prepared by prokaryotic expression strain,and the antigenicity of P protein was confirmed,which laid a foundation of the development of NV G Ⅱ.2[P16]detection kits and the recombinant subunit vaccines.

Objective To study the effect of articular step-off on knee joint contact pressures in fractures of the medial tibial plateau. Methods Eight specimens of embalmed adult knee joint were used to establish models of articular step-off of the medial tibial plateau by 0,1,2,3 mm respectively (Schatzker Ⅳ fracture) .The technique of pressure sensitive film was used to measure the contact pressures on the weight-bearing area of the knee joint in the 3 models. Results When the step-offs of medial tibial plateau were 0,1,2,3 mm,the average contact pressures on the lateral compartment were respectively 3.34 ± 0.06 MPa,3.48 ± 0.06 MPa,4.19 ± 0.05 MPa,4.19 ± 0.04 MPa,while the average contact pressures on the medial coMPartment were 4.04±0.17 MPa,4.57±0.20 MPa,5.64±0.20 MPa,5.64±0.19 MPa.When the step-off of medial tibial plateau was 2 mm,the average contact pressures on the medial and lateral plateaus increased by 39.6％ and 25.4％ compared with the 0 mm step-off,with statistically significant differences (P ＜0.05). Conclusions If the step-off of the medial tibial plateau reaches ≥ 2mm,open reduction and internal fixation should be indicated to prevent secondary traumatic arthritis.When the step-off of the medial tibial plateau is ＜ 2 mm,conservative treatment can be a proper choice.

Objective To observe the clinical effect of modified Hezhong Powder for the treatment of children mesentetic lymphadenitis.Methods Sixty children with mesentetic lymphadenitis were randomly divided into treatment group and control group,30 cases in each group.The control group was treated routine anti-inflammatory drug Cefaclor for Oral Suspension,and was given symptomatic treatment when necessary.The treatment group was given modified Hezhong Powder on the basis of treatment for the control group.The treatment course lasted for two weeks.After treatment,clinical efficacy and safety,and effects on relieving primary and secondary symptoms and on shortening the swollen lymph nodes were observed.Results (1) The total effective rate of the treatment group was 96.7％,and that of the control group was 83.3％,the difference being significant (P ＜ 0.05).(2) The treatment group had better effect on relieving the primary symptom of abdominal pain,and secondary symptoms of poor appetite,vomiting,abdominal distention and abnormal stool than the control group.The differences of time for the symptoms disappearing were significant (P ＜ 0.05).(3) After treatment,the swollen.lymph nodes of the two groups were shrunk(P ＜ 0.05),and the shrinkage in the treatment group was superior to that of the control group (P ＜0.05).(4) During the treatment,no obvious adverse reaction occurred in the two groups.Conclusion Modified Hezhong Powder combined with western medicine is effective and safe for the treatment of children mesentetic lymphadenitis,and its efficacy is superior to that of the western medicine alone.

Objective To investigate the clinical efficacy and safety of laparoscopic appendectomy for acute appendicitis.Methods Nighty-two cases of acute appendicitis were included in this study,randomly divided into observation group (n =46) and control group (n =46) according to the random number table method.The observation group was treated with laparoscopic appendectomy,and control group with the traditional open appendectomy treatment.We compared the two groups of cases in postoperative conditions and the level of WBC changes and 1d and 3d after operation.Results The operation time of the observation group was shorter than that of the control group (t=4.049,P＜0.05);there was no significant difference in lymph nodes (t=0.570,P＞0.05);the amount of bleeding was less than that of the control group (t=20.748,P＜0.05).The get-out-of-bed activity time in the observation group was faster than the control group (t=9.221,P＜0.05);eating time was faster than the control group (t=8.794,P＜0.05);the anus recovery exhaust time was faster than the control group (t=17.423,P＜0.05);hospital stay was shorter than the control group (t=13.479,P＜0.05);the complication rate was lower than that of the control group (t=13.883,P＜0.05).The levels of 1d and 3d WBC were lower in the observation group than that in the preoperative group (t=7.277,26.911,P＜0.05);the levels of 1d and 3d WBC in the observation group were lower than those in the control group at the same time (t=3.6189,17.827,P＜0.05);the 3d WBC level of the observation group was lower than that of 1d (t=20.732,P＜0.05).Conclusion Laparoscopic appendectomy is a safe and effective treatment for patients with acute appendicitis,and it is worthy of further clinical research.

The medicine of Yi ethnic plays an important role of the traditional medicine of China. Recently, the facility of Yi ethnic medicine has made great progress on its development process. Now many medicine industries develop the production of Yi ethnic medicine. Thus, this demands a great quantity of Yi ethnic medicine, but it also leads rapid decline with overuse of the medical resources. Here we discussed a series of problems, and put forward the corresponding stradegies of protection of Yi medicine.

Objective] To sum up Pro. Li Saimei’s clinical experience in treating mental diseases. [Method] By following the teacher clinic and sorting out the related medical materials,the author summarizes professor Li Saimei 's academic experience of treatment of mental diseases ,and for proven cases. [Result] In professor Li Saimei's opinion,mental diseases are due to dysfunction of shaoyang's turning,and phlegm blocking the spirit orifices. Professor Li usually uses Chaihu related formulas to turn the Shaoyang,resolve the phlegm,open the three Jiao,regulate Yin and Yang.The notable clinical result has been observed.[Conclusion] Professor Li Saimei's experience is effective and worthy of inheritance and promotion.

Objective To investigate the predictive value of epicardial adipose tissue(EAT) thickness for the recurrence of atrial fibrillation (RAF) after concomitant valve replacement and bipolar radiofrequency ablation.Methods From January 2012 to May 2014,156 patients with permanent atrial fibrillation and organic valvular diseases underwent concomitant valve replacement and successful bipolar radiofrequency ablation in the First People's Hospital of Shangqiu.After 1 year of follow-up,the recurrence of AF was analyzed and the correlation factors were analyzed statistically.Results All patients completed a regular follow-up.During a 12-month follow-up period,RAF was observed in 39 patients (25.0％).Echocardiography-derived regional EAT thickness was significantly greater in RAF patients than that in non-RAF patients((6.9±0.6) mm vs.(5.5±0.7) mm,t=4.649,P＜0.05).The EAT thickness strongly correlated with patients age,body mass index,Ratio of left atrial diameter,mitral diastolic blood flow velocity and early diastolic velocity of mitral annulus,low density lipoprotein-cholesterol and thyroglobulin (r =0.62,0.57,0.68,0.59,0.73,0.76;P ＜ 0.05).Cox multivariable analysis showed that,duration of AF history (95％CI:1.01-1.15,P =0.040),left atrial diameter (95％CI:1.07-1.24,P =0.021) and EAT thickness (95％CI:1.05-1.79,P =0.009) were independent predictors of RAF.Conclusion Echocardiography-derived regional EAT thickness combined with left atrial diameter may serve as effective parameters in identifying patients at the highest risk for RAF.

Objective To study the pathogenic molecular mechanism of H.pylori through analyzing its effect of on MMP-9 expression. Methods BGC-823 cells were cocultured with ATCC49503(CagA +)and HP 030811 (CagA -) H.pylori strains respectively, then the total cellelar RNA was extracted. The MMP-9 mRNA expression in BGC-823 cells, 9 cases of H.pylori-positive and 9 cases of H.pylori-negative gastric ulcer biopsy tissues was detected by RT-PCR. The MMP-9 protein expression on the paraffin-imbedded tissue sections of 17 cases of H.pylori-positive and 14 cases of H.pylori-negative gastric ulcer tissues was detected with immunohistochemical technique, and was quantified by image quantitative analysis. Results H.pylori induced MMP-9 expression in BGC-823, and the level of MMP-9 expression induced by CagA -postive H.pylori strain was higher than that by CagA -negative one. MMP-9 expression level was higher in the H.pylori-positive gastric ulcer tissues than that in the H.pylori-negative ones. Conclusion H.pylori-induced MMP-9 expression may play an important role in gastric ulcer formation and gastric carcinogenesis.