AIM:To establish a method for determining notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Jingkang Capsules(Radix Rehmanniae praeparata,Radix Polygoni multiflori,Radix et Rhizoma Notoginseng,etc.). METHODS: A Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m) with acetonitrile-water as the mobile phase was used for gradient elution,the flow rate was 1.0 mL/min and the UV detection wavelength was at 203 mn. RESULTS: There was a good linear relationship at a range of 0.327-2.180 ?g for notoginsenoside R_1(r=(0.999 4))(the average recovery was 98.23% with RSD of 1.53%(n=6)),0.909-6.060 ?g for ginsenoside Rg_1(r=1.0)(the average recovery was 99.04% with RSD of 1.00%(n=6)),0.672-4.480 ?g for ginsenoside Rb_1(r=0.999 8)(the average recovery was 98.17% with RSD of 1.31%(n=6)).CONCLUSION: The method is simple,quick and accurate.It can be used for the quality control of Jingkang Capsules.

BACKGROUND: With the development of technology, poly-L-lactic acid/β-tricalcium phosphate composite materials show good characters in the tissue engineering, which can promote osteoblast proliferation, reduce rejection reactions, and improve bone healing in a dose-dependent manner. OBJECTIVE: To test the influence of poly-L-lactic acid with different molecule weights on the structure and function of poly-L-lactic acid/β-tricalcium phosphate composite scaffolds. METHODS: Poly-L-lactic acid with molecule weights of 200 000 and 380 000 were combined with β-tricalcium phosphate to produce composite scaffolds by using freeze-drying method. Porosity and pore size of the samples were measured. The fetal rabbit bone mesenchymal stem cells (BMSCs) were cultured and expanded in vitro. They were harvested and seeded into the prepared poly-L-lactic acid/β-tricalcium phosphate scaffolds. The 3-(4, 5-Dimethylthiazol-2yl)-2, 5 diphenyltetrazolium bromide (MTT) and alkaline phosphatase were examined for comparison between normal cultured BMSCs and those cultured on the different poly-L-lactic acid/β-tricalcium phosphate scaffolds. RESULTS AND CONCLUSION: Images of scanning electron microscope showed that the cells adhered to the scaffolds greatly. The value of MTT and alkaline phosphatase showed no significant difference (P > 0.05). The molecule weight of poly-L-lactic acid has no influence on the biological function of composite materials.

OBJECTIVETo investigate the clinical therapeutic effects of anchors, peroneus brevis tendon augmentation and plantaris muscle tendon covering on the reconstruction of achilles tendon rupture caused by corticosteroids injection.

METHODSFrom March 2005 to April 2010, the clinical data of 10 patients with acute achilles tendon rupture repaired with suture anchors, peroneus brevis tendon augmentation and plantaris muscle tendon covering were retrospectively analyzed. The achilles tendon rupture was caused by corticosteroids injection. There were 8 males and 2 females with a mean age of (46.80 +/- 2.83) years old(ranged from 21 to 68 years). Postoperative complications, the range of movement of affected foot, number of consecutive heel raises and single leg jumpings were recorded. Functional recovery of achilles tendon were assessed according to ankle and hindfoot scores of the American Orthopedic Foot Ankle Society (AOFAS).

RESULTSAll patients were followed up for 12 to 18 months with an average of 13.5 months. No wound infection, re-rupture and rejection reaction were found. At the last follow-up, there was no significant difference in the range of movement between affected foot (54.5 +/- 6.3) degrees and unaffected foot (56.8 +/- 3.8) degrees (t = 0.989, P = 0.336). The affected foot could raise heel and do single-leg hops for 10 times continuosly. There was significant difference in AOFAS between preoperative score (67.3 +/- 7.6) and postoperative score (95.5 +/- 7.6) (t = 8.297, P = 0.000);and there was no significant difference between affected foot scores (95.5 +/- 7.6)and unaffected foot scores (98.5 +/- 6.3) (t = 0.961, P = 0.349). Function recovery of achilles tendon: 9 cases were good, 1 case was fine.

CONCLUSIONAnchors, peroneus brevis tendon augmentation and plantaris muscle tendon covering for the reconstruction of achilles tendon rupture caused by corticosteroids injection is a reliable and effective method, with advantage of simple operation, dependable fixation and less complications.

Achilles Tendon ; injuries ; physiopathology ; surgery ; Adrenal Cortex Hormones ; adverse effects ; Adult ; Aged ; Female ; Humans ; Injections ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Recovery of Function ; Rupture ; surgery ; Tendon Injuries ; physiopathology ; surgery

Objective To study the effect of chitosan/tricalcium phosphate(CTCP)on the attachment and proliferation of bone marrow stromal cells(BMSCs)cultivated in vitro and explore their biocompatibilities.Methods The CTCP was prepared by freeze-drying.BMSCs were cultivated in vitro,collected and seeded onto the surface of CTCP.Cell attachment and proliferation were obesrved using inverted light microscope and scanning electron microscopy(SEM).The cell proliferation was tracked by MTT method 2,4,6,8 d after seeding.Results BMSCs were fibroblast-like 2 d after seeding.The mean number of cells was(360?20) and(76?18),respectively,in the experimental and control group per 100 eyesight field,the difference was significant(P

Objective:To investigate the relationship between marriage/loving affairs related life events and the high suicide rates among Chinese rural women.Methods: 66 suicides and 66 living controls were studied using psychological autopsy methods.Results:There were 36 women in the research subjects which account for 27% of the total sample (36/132). There were more marriage/loving affairs related life events in female suicide than in other groups (1.6?2.1 vs. 1.0?1.5,0.2?0.6,0.6?0.9) and in female suicide the frequency of marriage/loving affairs related life events is higher than the frequency of other four types of life events (1.6?2.1 vs. 0.5?0.7,0.4?0.6,0.9?0.9,0.4?0.9). Both of the two differences do not have statistical significance.Conclusion:More attention should be paid to marriage/loving affairs related life events for Chinese rural women.

Objective To construct and identify recombinant vaccine Bifwlobacterium bifidum(Bb)pGEX-Sj14-3-3 of Schistosoma japonicum(Sj). Methods Total RNA was extracted from adult Sj, antigen encoding gene Sj14-3-3 was amplified by RT-PCR and cloned into Escherichia coli (E. coli)-Bb shuttle expression vector pGEX-1λT to construct recombinant plasmid pGEX-Sj14-3-3. The recombinant plasmid was transformed into E. coli BL21 (DE3).The plasmid was extracted and identified by using BamH I and EcoR I. Then pGEX-Sjl4-3-3 was electroporated into Bb to construct recombinant Bb (pGEX-Sj14-3-3) vaccine. The extracted plasmid of the recombinant Bb (pGEX-Sj14-3-3) vaccine was identified by PCR, and the size of the products was compared with Sj14-3-3 gene of adult worms.Results Sj14-3-3 of 399 bp in length was amplified by RT-PCR. The products were digested by BamH I and EcoR I , and the fragments length of plasmid pGEX-Sj14-3-3 vector was 4947 bp, and of Sj 14-3-3 gene was 399 bp.The product of 399 bp Sj14-3-3 gene was also amplified by PCR from template of the extracted plasmid of the recombinant Bb(pGEX-Sj14-3-3 ) vaccine. The size of the product obtained was just the same as expected.Conclusion The recombinant Bb(pGEX-Sj14-3-3) vaccine of Sj is successfully constructed.

Objective To investigate the effects of recombinant vaccine Bifidobacterium bifidum (Bb) pGEX-Sj14-3-3 on splenocyte apoptosis in BALB/c mice.Methods Ninety-six BALB/c mice were randomly divided into two groups according to their body mass:per os group (PO) and intranasal immunization group (IN),with 48 mice in each group.All mice were orally and intranasally immunized with recombinant vaccine Bb(pGEX-Sj14-3-3).Four mice in each group were sacrificed on weeks 0,2,4,6,8,10,12,14,16,18,20 and 22,respectively,after immunization,and splenocytes were separated and cultured with or without ConA stimulation.The apoptotic rates of splenocytes were detected by flow cytometry.Results It showed that apoptotic level of splenocytes in both groups remarkably increased after 2-4 weeks without ConA stimulation (PO:0.069 ± 0.005,0.076 ± 0.010; IN:0.037 ± 0.002,0.075 ± 0.002),and the value reached the peak on the 4th week,and the differences were statistically significant compared with that of week 0(all P ＜ 0.05).Apoptotic level of splenocytes in both groups with ConA stimulation increased after 2-6 weeks(PO:0.089± 0.006,0.098 ± 0.010,0.060±0.007; IN:0.054 ± 0.001,0.093 ± 0.003,0.058 ± 0.012),and the value also reached the peak after 4 week,respectively.The differences were statistically significant compared with that of week 0 (all P ＜ 0.05).Apoptotic level of splenocytes in each group with ConA stimulation was significantly higher than that without ConA stimulation.Conclusion It is suspected that the recombinant vaccine Bb(pGEX-Sj14-3-3) may inhibit apoptosis of splenocytes in mice immunized orally or intranasally.

ObjectiveTo study the dynamic changes of IgG,IgG subclasses,IgE and IgA in sera of BALB/c mice immunized with recombinant Bifidobacterium bifidum (Bb) pGEX-Sj14-3-3 vaccine of Schistosoma japonicum.MethodsNinty six BALB/c mice were randomly divided into two groups:oral immunization group and intranasal immunization group,48 mice in each group.Mice were orally and intranasally immunized with recombinant Bb(pGEX-Sj14-3-3) vaccine,respectively.Four mice from each group were sacrificed,respectively,on weeks 0,2,4,6,8,10,12,14,16,18,20 and 22 after immunization and their sera from the eyeballs were collected.The levels of IgG,IgG subclasses,IgE and IgA were assayed with routine Enzyme-linked immunosorbent assay(ELISA).ResultsThe titers of IgG,IgG1,IgG2a,IgG2b,IgG3,IgE and IgA in both groups increased during the 2 - 22th,2 - 14th,2 - 22th,2 - 22th,2 - 20th,2 - 22th,2 - 22th weeks,respectively.The values reached the highest level on weeks 8,6,6,4,8,10 and 6,respectively,in the oral group,and the values were (0.065 ±0.001,0.021 ± 0.002,0.011 ± 0.001,0.015 ± 0.003,0.014 ± 0.002,0.011 ± 0.001,0.013 ± 0.002),respectively,as compared with the values on week 0(0.032 ± 0.001,0.015 ± 0.002,0.005 ± 0.002,0.005 ± 0.001,0.006 ± 0.001,0.006 ± 0.001,0.005 ± 0.001 ),the differences were statistically significant(P ＜ 0.05 or ＜ 0.01 ) except that of IgG1 and IgG2b.In the intranasal group these values reached the highest levels on weeks 4,6,4,4,8,10 and 8,respectively,and the values were (0.064± 0.003,0.022 ± 0.002,0.012 ± 0.003,0.019 ± 0.001,0.013 ± 0.001,0.015 ± 0.001,0.014 ± 0.003),respectively,as compared with the value on week 0,the differences were statistically significant(P ＜ 0.05 or ＜ 0.01 ) except that of IgG1 and IgA.ConclusionsTypes Th1 and Th2 mixed type immune responses can be induced in mice by immunization with the recombinant Bb(pGEX-Sj14-3-3) vaccine by early period of immunization (2th - 10th week).

Objective:To study the impact of smoking on C-reactive protein(CRP)concentration in gingival crevicular fluid(GCF) before and after initial treatment.Methods:18 smokers and 18 non-smokers with moderate or severe periodontal disease were recrui-ted into this study.The clinical indexes of periodontal examination of the patients were recorded,GCF samples of the patients were collected,CRP level in the samples was measured by radioimmunoassay balance method before and 4 weeks after initial treatment. Results:4 weeks after initial treatment,the clinical indexes and CRP concentration in GCF of the smoking group and non-smoking group were significantly lower than those before treatment(P <0.05),the changes of the smoking group were less than those of the non-smoking group(P <0.05).Conclusion:Smoking is an unfavorable factor of the initial periodontal therapy.

Objective To investigate the daily intake of dietary Cu and Mn and to study the serum concentration reference values of copper and manganese of healthy adults in Ji'nan urban area.Methods From August 2007 to January 2008,1 005 Ji'nan urban healthy subjects were recruited for filling in seven days dietary intakes questionnaire to investigate the dietary Cu and Mn daily intake.Then atomic absorption spectrometry was applied to determine the concentration of serum Mn and Cu after the samples were digested by microwave.Results The dietary daily intake of Cu and Mn of Ji'nan urban healthy adults were 1.78 mg and 4.21 mg respectively.Cu intake of male was higher than that of female(P