Tg gene polymorphism, HLA and iodine interaction with Tg may be involved in the pathogenesis of autoimmune thyroid disease.Moreover, thyroglobulin antibody (TgAb), an important symbol of autoimmune thyroid disease, is also closely related to the development of autoimmune thyroid disease.In addition, the differences of TgAb IgG isoforms expression level also have broad application prospects in the classification and diagnosis of autoimmune thyroid disease.By researching on Tg, we review the pathogenesis of autoimmune thyroid disease and provided the evidence for its diagnosis and clinical treatment.

To prepare monoclonal antibodies for blocking acute hemorrhagic conjunctivitis (AHC)viruses,BALB/C mice were immunized with Hela cells.The spleen of immunemouse was re-moved,and spleen cells were fused with SP2/0 cells.The antibodies against cell receptors wereselected by cell protection assay.Four cell strains secreted McAbs designated R7.R8.R16 andR24 were screened to be able to block cellular receptors and inhibit the infection of Ev70 as wellas CA24v on Hela cell.These results demonstrate that the receptors for Ev70 and CA24v are si-miliar.Indirect immunofluorescence test with the four McAbs showed definite fluorescence onliving Hela cells,which the intensity was concordance with the mean intensity resulted fromflowcytometer.Positive reaction on fixed Hela cells were only observed with R7 and R8.

Radiotherapy is one of the main treatment options for patients with advanced limited non small cell lung cancer.From the viewpoint of radiobiology,the change of fractionation schedule in radiotherapy might improve the therapeutic effect.There are many different methods of fractionated irradiation: from conventional radiation including hyperfractionated radiotherapy,accelerated radiotherapy and hypofractionated radiotherapy,etc.In this paper,we summarized the definition of the different types of fractionated irradiation, and its clinical application.

Anemia, Iron-Deficiency ; complications ; Female ; Humans ; Middle Aged ; Telangiectasia, Hereditary Hemorrhagic ; complications

Objective To objectively evaluate the intrusion effects on the maxillary incisors of implants versus conventional method(J-hook headgear ,utility arch) .Methods Such online databases as Cochrane library ,PubMed ,EMbase ,VIP ,Wanfang and CNKI were searched by randomized control test and prospective case controlled study about implants vs conventional anchorage for the maxillary incisor intrusion .After study selection ,assessment and data extraction conducted ,meta-analyses were performed by u-sing the RevMan 5 .1 software .Results Totally 11 studies were included ,6 studies involving 154 patients were included in the J-hook headgear group ,5 studies involving 127 patients were included in the utility arch group .The results of meta-analyses showed that :(1)Compared with the J-hook headgear ,implants had more decrement in the distance of upper center incisior cutting to palatal plane(P<0 .001) ,more decrement in overbite(P=0 .001) ,shorter treatment time (P<0 .001) ,less increase in SN-OP angle(P=0 .004) ,less decrement in distance of maxillary molar cusp to palatal plane (P=0 .020);There was no statistically significant differ-ence in overjet ,root resorption(P>0 .05) .(2)Compared with the utility arch ,implants had more decrement in the distance of the center of resistance of the upper incisor to palatal plane (P=0 .010) ,less increase in U6-PP angle (P<0 .001);There was no statis-tically significant difference in the distance of upper center incisior cutting to palatal plane ,U1-PP angle ,the distance of upper first Molar cutting to palatal plane ,sagittal position of the mesial tip of the upper first Molar ,overbite ,overjet ,course of treatment ,root resorption(P>0 .05) .Conclusion The implants anchorage intruded the maxillary incisor was better than conventional method (J-hook headgear ,utility arch) ,it has more incisor intrusion ,shorter treatment time and more comfortable for the patients ,there was no difference in root resorption between the two groups .

Purpose:To investigate fMRI and DTI in the assessment of cortical visual impairment in children with periventricular leukom-alacia ( PVL).Materials and Methods: Twenty-four children with PVL were enrolled.Meanwhile,24 age-matched normal controls were recruited for comparison.fMRI scan was performed using a 3.0T MR scanner.Data analysis was performed by statistical parametric mapping software (SPM2).Activated voxels were identified in both groups,t test was used for statistical analysis.DTI was performed by MedlNRIA software and DTI color maps were created from fractional anisotropy (FA) values and the three vector elements,FA values on diffusion tensor color maps were compared between the patients and the controls.AH the FA values of these WM fibers were analyzed by paired t test.The correlation was calculated between FA values and activated voxels of visual cortex for PVL children using SPSS10.0.Results: In all 24 normal children,the maximum response of blood oxygen level-dependent (BOLD) signal was located in the primary visual cortex ( PVC).However,some of the 24 cases of PVL had deviated activation.The active voxels of patients in visual cortex were less than that of controls.All 24 cases of PVL showed a significant mean FA reduction in ICPL and PTR in comparison to the ipsilateral regions of healthy controls.The significantly positive correlation was shown between FA values and activated voxels of visual cortex for PVL children.Conclusion:fMRI and DTI play an important role in the assessment of cortical visual impairment in children with PVL.

Objective To investigate the correlations between vascular dementia and neurotrophin-3 (NT-3), fibroblast growth factor (FGF). Methods Female and male Wistar senile rats were divided into the control group and the operation groups at random. The operation groups consisted of the cerebral ischemia 15 min group and ischemia 15 min reperfusion groups in which the rats were reperfused at 0, 1st, 6th hour and on 2nd, 4th, 9th day with five rats per group. The expressions of NT-3 and FGF in hippocampus and thalamus were examined in different periods of ischemia-reperfusion with ABC method of immunohistochemistry. Results There was a little expressions of NT-3 and FGF in hippocampus and thalamus of normal senile rats; the expression of NT-3 in hippocampus increased quickly and steadily from 1 h to 4 d after ischemia-reperfusion! there were significant differences compared with control group (P0. 05). The expression of FGF in hippocampus increased from 6 h to 2 d after ischemia-reperfusion (P 0. 05). FGF in thalamus began to increase on 2nd day after ischemia-reperfusion and there were significant differences compared with control group (P0. 05). Conclusion Hippocampus has the protective mechanism of NT-3 and FGF against ischemia injury which is produced quickly and has endurable effects, but thalamus lacks the good protective mechanism of NT-3 and FGF.

Adrenomedulin is an angiogenesis peptide that was found recently.It plays a critical role as an autocrine/paracrine tumor cell survival factor.Recently,adrenomedulin has been proved that it is overex- pressed in tumor,such as breast cancer.It may have a significant role in tumor progression and differentiation by stimulating mitogenic activities,inhibiting immune response,inhibiting apoptosis and enhancing angiogen- esis.We may realize targeted therapy tumor by intervening adrenomedulin,its receptor,and the signal con- duction pathway.

Objective To explore the cytotoxicity of the cytotoxic T lymphocyte (CTL) induced by SW480 sonicate sensitized dendritic cells (DC) on the colon cancer cell line SW480. Methods PBMC were separated from the HLA-A*0201 donor and DC were cultured with rhGM-CSF, rhIL-4 and rhTNF-α. The same donor's primary CTL were stimulated by DC loaded with SW480 sonicate. The cytotoxicity of CTL on SW480 (HLA-A*0201 positive) and K562 (HLA-A*0201 negative) was determined by the MTT method. Results The cytotoxicity of the CTL on SW480 was stronger than that on K562 (P ＜0.05). Conclusion The DC vaccine can stimulate specific CTL which can trigger cytotoxic activity on the target cells and this cytotoxicity is related to MHC restriction.

Objective To explore the clinical effect and prognosis of plasma N -terminal probrain natriuret-ic peptide (NT -proBNP)and 6 min walk test performance (6 -MWT)in patients with chronic heart failure (CHF).Methods 100 patients with CHF were selected as observation group,and 50 healthy people were selected as control group.They were grouped by NYHA degree,28 cases were NYHA Ⅱ,56 cases were NYHA Ⅲ and 16 cases were NYHA Ⅳ.The plasma level of NT -proBNP,6 -MWT,left ventricular ejection fraction (LVEF),early diastolic peak mitral flow velocity to late diastolic peak mitral flow velocity (E /A)were measured.Results The plasma NT -proBNP level of CHF patients was positively correlated with NYHA degree (r =0.683,P <0.01);6 -MWT was neg-atively correlated with NYHA degree(r =-0.518,P <0.01).For CHF patients,significant correlation was observed between NT -proBNP and 6 -MWT (r =-0.789,P <0.05).Plasma NT -proBNP level was correlated with dias-tolic HF,systolic HF and mixed type heart failure (r =-0.678,P <0.01;r =-0.845,P <0.01;r =-0.759,P <0.01).Conclusion Plasma NT -proBNP,6 -MWT can serve as a marker for the detection and evaluation of heart failure.Meanwhile,the higher the NT -proBNP level,the shorter the 6 -MWT,the more serious the heart failure,the poorer the prognosis.