1.Effects of nonverbal behavior on anesthesiology teaching
Chinese Journal of Medical Education Research 2015;(5):489-491
As the burden of anesthesiology teaching is heavy in undergraduate course, teachers can communicate with the students by nonverbal behaviors, such as eye contact, silent language, facial expression, paralanguage, which can make class atmosphere active, develop harmonious teacher-student relationship, and improve the quality of class teaching. Teachers of anesthesiology should take some measures, such as rehearsing lessons, summing up the experience of the lessons in time, accu-mulating the experience of nonverbal behaviors, and doing some exercise for the nonverbal behavior as much as possible, so as to set up an excellent atmosphere for teaching ,and meanwhile improve the quality of anesthesiology teaching.
2.Application Status and Evaluation of Combination Vaccines
Jun REN ; Jian-Min LI ; Wei CHEN ;
China Biotechnology 2006;0(11):-
A combination vaccine which consists of ≥2 immunogens is intended to provide protection against two or more diseases or against multiple serotypes of a single disease. The use of combination vaccines decrease the number of vaccine injections in the regular immunization schedules. However,the development of combination vaccines is more complicated than monovalent vaccines,preservatives and adjuvants used with one component may alter the potency of other components. Physical,chemical,and biological interactions between the components of combination vaccines must be considered to avoid detrimental effects on safety or efficacy. Therefore combination vaccines present unique challenges for product evaluation. This paper presents a review of research application status,the evaluation of effectiveness and safety,as well as development prospects on combination vaccines.
3.Fifty cases of dyspnea treated by warming needle moxibustion.
Ren-Ding WU ; You-Hong LI ; Jing-Min SONG
Chinese Acupuncture & Moxibustion 2012;32(9):856-857
Acupuncture Therapy
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instrumentation
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Combined Modality Therapy
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Dyspnea
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therapy
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Female
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Humans
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Male
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Middle Aged
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Moxibustion
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Young Adult
5.Study of left ventricular diastolic function in hypertensive patients with different left ventricular geometric patterns by vector flow mapping
Hongfeng WANG ; Min REN ; Kaikai SHEN ; Hairu LI ; Jiawei TIAN
Chinese Journal of Ultrasonography 2016;25(11):926-931
Objective To assess the clinical application value of vector flow mapping (VFM) in evaluating the left ventricular diastolic function in hypertensive patients with different left ventricular geometric patterns.Methods One hundred and sixty-nine patients with hypertension and 50 healthy volunteers were enrolled.Hypertensive patients were divided into four groups with different ventricular configurations according to ASE guide line in 2005:normal geometry group,concentric remodeling group,concentric hypertrophy group and eccentric hypertrophy group.The quantitative parameters,including average energy loss(aEL) and circulation were measured.Difference was evaluated among five groups at apical,mid and basal segments and at different periods.The correlationship was analyzed between aEL,circulation and E/e',respectively.Results ① Compared to control group,aEL was higher in four hypertension groups at three segments of atrial systole(P <0.05).aEL in eccentric hypertrophy group was higher than those in control group,normal geometry group or concentric remodeling group at apical and mid segments of atrial systole,and higher than those in other hypertensive groups at basal segment (P < 0.05).In four hypertension groups,circulations in rapid filling phase of anterior mitral valve were lower than that in control group,but circulations in slow filling phase of posterior mitral valve were higher than that in control group(P <0.05).The circulation in slow filling phase of posterior mitral valve in concentric remodeling group was higher than that in normal geometry group,and that in eccentric hypertrophy group was higher than those in normal geometry group and concentric remodeling group(P <0.05).②When intra-group comparison,aEL were different in different segments of same phase or at different phases of same segment(P <0.05).③aEL had a good correlation with E/e'at basal segment of atrial systole in four hypertension groups(r =0.66,P <0.01).Conclusions VFM can be utilized to analyze left ventricular hemodynamics features of hypertensive patients with different left ventricular geometric patterns,it may be a good supplement for assessing cardiac diastolic function and reflect the extent of damage.
6.Progress in research & development of interferon-antibody conjugates
Chenchen LI ; Xueyan REN ; Min WANG ; Juan ZHANG
Chinese Journal of Biochemical Pharmaceutics 2016;36(6):19-24
Interferon ( IFN) kills tumor through:direct tumor killing, stimulating immune response to tumor cells by activating the immune system and inhibiting tumor angiogenesis.However, its clinical application has been restrained by the deficiencies such as short half-life and severe side effects.IFN-antibody fusion can bring IFN to the vicinity of tumors using tumor specific antibody, while reducing the concentration of IFN in the rest parts of the body.Thus, system toxicity caused by IFN can be significantly lessened.We herein summarized the research and development of preclinical antibody-interferon conjugates and provide new thought on the development of IFN or antibody based fusion proteins.
7.Evaluation of left ventricular global and regional systolic function in patients with systemic lupus erythematosus ;by three-dimensional speckle tracking imaging
Lutong ZHANG ; Hairu LI ; Xudong WANG ; Min REN ; Jiawei TIAN
Chinese Journal of Ultrasonography 2015;(6):472-475
Objective To evaluate left ventricular(LV)global and regional systolic function by three-dimensional speckle tracking imaging (3D-STI)in patients with systemic lupus erythematosus (SLE). Methods Twenty-two patients with SLE were selected randomly as SLE group and twenty-two healthy people were included as control group.3D-STI was performed in all participants to get global and regional strain parameters,including global longitudinal peak systolic strain(GLS),global circular peak systolic strain (GCS ),global radial peak systolic strain (GRS ),global area peak systolic strain (GAS ),segmental longitudinal peak systolic strain (SLS),segmental circumferential peak systolic strain (SCS),segmental radial peak systolic strain (SRS),and segmental area peak systolic strain (SAS).The sensitivity and specificity of GCS and GAS reflecting LV systolic function in patients with SLE were analyzed under the receiver operating characteristic (ROC)curve.Results ①The global strain:compared with the control group,GCS and GAS decreased significantly in SLE group (P <0.01),there was no significant change in GLS and GRS(P >0.05).②The regional strain:compared with the control group,the SCS in segments of basal anteroseptal,basal inferoseptal,mid anterior,mid inferosptal,mid anterolateral,apical inferior,apical lateral decreased significantly in SLE group.The SAS of basal anterior,basal inferoseptal,mid inferosptal, mid anterolateral,apical anterioe,apical lateral decreased significantly in SLE group (P <0.01).The SCS in segments of apex and the SAS of mid anterior,apical septal,apex decreased in SLE group (P <0.05).③The ROC curve:the sensitivity and specificity of GCS on detecting the LV systolic dysfunction were 59.1%and 87.5%,and those of GAS were 77.3% and 83.3%,respectively.Conclusions 3D-STI is a convenient and accurate way to early detect LV global and regional systolic dysfunction in SLE patients.
8.Inhibition of ginkgo flavones against aggregation and fibrogenesis of A?_(1-42)
Guangwu LI ; Zhenhua REN ; Peiyuan WANG ; Min TANG ; Huaqiao WANG
Chinese Traditional Patent Medicine 1992;0(06):-
AIM:To gain the molecular mechanism of active ingredients of EGb 761(ginkgo flavones and ginkgo lides) for the inhibition against A?_(1-42) aggregation and fibrogenesis.METHODS:Circular dichroism spectra(CD) and FI-IR were used to curve-fit and analyze the change in A?_(1-42) second structure under A? aged condition and intervention of ginkgo flavones and ginkgo lides.RESULTS:Analysis that was made of 1700-1600 m(-1) amide-Ⅰ-band and curve fitting indicated that after 30 min to 72-incubation,increase of ?-sheet in A?_(1-42) by(18.5%),but the reduction of ?-sheet by(50.95%) and(36.09%) in 72 h incubation with ginko flavones and ginkgo lides,respectively.And increase of ?-turn by(56.56%) and(46.56%) in sane condition.CONCLUSION:Obviously,?-sheet turned into ?-turn.-CH_2 and-CH groups of ketones and alkanes engaged in A?_(1-42) molecular change.
9.Expression of ER,CyclinD1 in glandular tissue of pubertal mammary hypertrophy
Min REN ; Ying WANG ; Li TENG ; Benzhong WANG
Journal of Endocrine Surgery 2009;3(5):304-307
Objective To investigate the expression of the ER,CyclinD1 in breast tissue of pubertal mammary hypertrophy and explore the possible etiology of pubertal mammary hypertrophy.Methods The expression of ER,CyclinD1 was detected by Western-blot in 15 cases of pubertal mammary hypertrophy,15 normal breast tissue and 10 cases of micromastia.Results There was significant difference of the expression of ER,CyclinD1 within breast tissue between pubertal mammary hypertrophy and micromastia(P<0.01).There was significant difference of the expression of ER,CyclinD1 within breast tissue in pubertal mammary hypertrophy and in normal breast tissue(P<0.01).There was no difference between the expression of ER,CyclinD1 within breast tissue in micromastia and in normal breast tissue(P>0.05).Conclusions The expression of ER,CyclinD1 in pubertal mammary hypertrophy are significantly higher than in micromastia and normal breast tissue.The pubertal mammary hypertrophy may be related to the expression status of ER,CyclinD1 within breast tissue.
10.Prokaryotic expression,purification and polyclonal antibody preparation of C terminal Helicase domain of mouse Rig-I
Jinju LI ; Hua REN ; Ziqiang WANG ; Min QIAN ; Bing DU
Chinese Journal of Immunology 2000;0(09):-
Objective:To develop a highly efficacious and sensitive immunological reagent for further investigation on the retinoic acid-induced gene I (Rig-I) of mouse .Methods:The Helicase domain coding region (726-2 240 bp) of mRig-I-H was cloned into plasmid pET15b (+) to construct the recombinant plasmid pET15b(+)-mRig-I-H.Then the plasmid was transformed into E.coli BL21 for protein expression.Rabbits were immunized with electrophoresis-purified recombinant protein to obtain the polyclonal antibody against mRig-I-H.The titer of polyclonal antibody was detected by ELISA and the specificity was identified by Western blot and Immunofluorescence.Results:The recombinant protein was expressed successfully in E.coli.Western blot analysis showed that target protein was expressed with a molecular weight of 40 kD.Titer of the polyclonal antibody was about 1∶1?105 by ELISA assay.With this antibody,we could detect the expression of Rig-I in RAW 264.7 cell line by Western blot and Immunofluorescence.Conclusion:The high level expression of Rig-I Helicase domain is induced in E.coli expressing system.Anti-mRig-I-H polyclonal antibody with high titer and fine specificity could be a novel tool in future investigation of Rig-I.