0.05.The neuronal necrosis area in group A are significantly higher than group B and C [(1.8?0.7)vs(0.9?0.4)and(0.6?0.3),both are P0.05.The neuronal necrosis area and the level of NSE in fetus were positively correlated with each other,r=0.798,P0.05.The volume of mitochondria of nerve cells in group A and B are significantly higher than that of group C [(7.0?1.8)?10~(-4)?m~3 and(5.7?1.6)?10~(-4)?m~3 vs(3.2?1.2)?10~(-4) ?m~3,both are P0.05). Conclusions There is apparente pathological change of fetal rats brain in cholic acid groups,the neuronal degeneration and the mitochondria swelling was mainly found in low cholic acid group,the neuronal necrosis and the mitochondria decrease was mainly found in high cholic acid group.The serum concentration of TBA and NSE in fetal rats were positively correlated with each other.

Objective To investigate the effects of 8Hz infrasound on spatial learning ability of rats and its the underlying mechanism. Methods Forty male SD rats were randomly divided into a control group and an experiment group (consisting of the 8Hz, 90dB, 100dB and 130dB subgroups). The experimental subgroups were exposed to 8Hz, 90dB, 100dB or 130dB infrasound for 2 hours daily, respectively, for a total of 6 days. All of the rats were taken out of the infrasonic depot once a day and given Morris maze training. And at last the brain of the rats were harvested and treated with immunohistochemical staining for detection of ryanodine receptor (RyR). The content of RyR in hippocampi and temporal cortex were detected under an optical microscope. Results The rats in the 8Hz, 90dB, 100dB and 130dB subgroups showed prolonged latency in Morris training ( P =0.010, P =0.001 and P = 0.000 ). The hippocampi and temporal cortex of rats in 8Hz, 90dB, 100dB and 130dB subgroups showed less positive neurons of RyR ( P =0.000). There was a negative correlation between the latency period of rats finding the hidden platform and the content of RyR in hippocampus and temporal cortex. Conclusion Infrasound of 8Hz, 90dB, 100dB and 130dB could decrease the spatial learning ability of rats, and this was possibly related to the reduce of RyR in hippocampus and temporal cortex.

Objective To study the effect of 8Hz infrasound on the expression of 5-HTR of hippocampus and temporal cortex in rats. Methods SD rats were exposed to 8Hz, 90dB,100dB or 130dB infrasound, respectively for 2 hours daily. Then the rats' brains were taken and given stained immunohistochemically for (5-hydroxy tryptamine receptor,5-HTR). The content of 5-HTR in hippocampus and temporal cortex were detected under an optical microscope. Results The animals in the infrasound groups had less expression of 5-HTR in hippocampus and temporal cortex, and the least were at the 35th day for 90dB group and 100dB group and the 28th day for 130dB group (P

The tumor microenvironment (TME), a dynamic and complex local environment, interacts with the tumor cells and is closely related to tumor growth, metastasis, immune escape and drug resistance. Thus, targeting the TME has been a worldwide focus in cancer therapy. Many natural products possess the advantages of multiple targets, multiple pathways and wide pharmacological functions, and are the main source of antitumor drugs. In recent years studies have found that some natural products had advantageous effects on the TME. In this review, we summarize the components and functions of the TME and some natural products that target the TME, with references to the drug therapy of cancer.

The present study was performed to explore the role of corticotropin releasing factor (CRF) in the nucleus basalis of Meynert (NBM) in spatial learning and memory of rats. The latency, distance and swimming path to find the platform were measured by Morris water maze after intra-NBM injections of 0, 0.01, 0.1 and 0.4 nmol of CRF. Intra-NBM injections of 0.1 or 0.4 nmol of CRF induced significant increase of the latency for spatial learning and memory, and there were no significant changes in the swimming speed in Morris water maze test. The results suggest that CRF plays an inhibitory role in spatial learning and memory consolidation in the NBM of rats.

Objective: To preare recombinant lentivirus stably suppressing PLC?1 in human colorectal carcinomas Lo-Vo cells, so as to establish LoVo cell lines deficient in PLC1 and to investigate the role of this gene. Methods: Recombinant lentivirus produceing PLC-?1 siRNA were prepared. After LoVo cells were transduced with lentivirust, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC?1 was examined by Western-blot and RT-PCR analysis. The effect of the lentivirus on the cell proliferation and cell adhesion was analyzed by XTT method and ctll adhesion assay, respectively. Results: PLC?1 siRNA knocked down PLC?1 expression in LoVo cells obviously. The silenced efficiency of siRNA transducted by recombinant lentivirus was very high. Adhesion of human colorectal carcinomas LoVo cell Lines was significantly decreased, while proliferation was not affected. Conclusion: Our research confirm that PLC?1 plays an important role in cell adhesion of colorectal carcinomas, and provides experimental evidences for targeting PLC?1 in gene therapy against cancer.

Objective: To describe the differences of plasma folate concentration and prevalence of folate deficiency in genders and ages in Chinese adults aged 35 to 64 years. Methods: By cross-sectional analysis, plasma folate concentration was measured in 2 545 adults including men and women by microbiological assay. Results: (1) Men tended to have lower plasma folate concentration and higher prevalence of folate deficiency(9.70 nmol/L, 31.0%) than did women(14.2 nmol/L, 12.5%, P=0.001). (2) Men in South have significantly higher plasma folate concentration and lower prevalence of folate deficiency than in North. The difference of plasma folate concentration was not evident between urban and rural area, but evident in North between in winter and spring. There were no significant differences of prevalence of folate deficiency for men between urban and rural areas in both regions. For women, the differences of plasma folate concentration in areas were significant, which were higher in South and urban, and lower in North and rural areas. (3) Plasma folate concentration increased with age in Southern men. A similar trend for others was not significant. Conclusion: Men have lower plasma folate concentration and higher prevalence of plasma folate deficiency than do women. The distribution of plasma folate concentrations in urban and rural areas seems different between men and women.

OBJECTIVE:To evaluate the merits and demerits of automatic oral drug dispensing mode as compared with traditional dispensing mode.METHODS:The medication data in our hospital after adoption of single dose administration system were collected and summarized analytically in respect of drug quality management.RESULTS:The automatic oral drug dispensing mode is superior to traditional dispensing mode.CONCLUSION:Under single dose administration system,the application of automatic oral drug dispensing mode is conductive to the improving of pharmaceutical care level.However,the problems existing in its clinical use remain to be further studied.

ObjectiveTo investigate effects of 8Hz 90dB and 8Hz 130dB infrasound on hippocampi ultrastructure of SD rats.Methods60 male SD rats were randomly divided into the control group and experimental group. Rats of experimental group were exposed to 8Hz 90dB or 8Hz 130dB infrasound 2 hours every day for 1,7,14, 21,28,35 and 42 days. As soon as the exposure finished,the right hippocampus of rat was taken and examined by electron microscopy.ResultsHippocampus ultrastructure of rats was changed after only one day exposure to infrasound and the longer exposure to infrasound, the worse ultrastructure changed. But during the latter period,damages of hippocampus ultrastructure became lighter. Different rules were observed between 90dB and 130dB groups.ConclusionInfrasound of 8Hz 90dB and 8Hz 130dB can make hippocampus ultrastructure of rats damaged mainly as denaturalizations. These changes are nonliner with time, and the hippocampus of rats has adaptability to infrasound.

Objective To verify the association of a disintegrin and metalloproteinase domain 33 (ADAM33) polymorphisms in childhood asthma susceptibility and severity in patients with moderate and severe asthma.Methods A total of 144 controls and 110 asthmatic patients were recruited for this hospitalbased case-control study.Two polymorphic sites (V4,T2) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method.The gene-gene interactions were analyzed with the multifactor dimensionality reduction (MDR) software.Results The allele frequencies of three SNPs (V4,T2) of ADAM33 in childhood asthma group were significantly higher than control group (P ＜ 0.01,OR =2.36 ～ 2.96,95％ CI:1.56-1.78 ～ 3.56-4.94).For the SNPs V4,GC genotype frequencies of both moderate and severe groups were significantly higher from the control group (P ＜ 0.05) ; the CC genotype frequencies in severe group were significantly higher than control group (P ＜ 0.05) ; the genotype GA of T2 locus in severe group and AA genotype frequencies in moderate group were significantly higher than control group (P ＜0.01 ～0.05) ; there were no significant differences for both allele and genotype frequencies of S2 locus between the childhood asthma and control group (P ＞ 0.05).By MDR analysis,the best interaction model was the four-factor model that the V4,T2 genotypes were the subgroup to predict asthma risk.Conclusions Our results highlight the role of ADAM33 as a susceptibility gene for childhood asthma,and the interactions among ADAM33 V4 and T2 are also associated with childhood asthma.