Gene Expression Profiling ; Humans ; Laser Therapy ; Lung Neoplasms ; genetics ; Microdissection ; methods ; RNA, Neoplasm ; isolation & purification

Objective To explore the extent of information needs and influencing factors among breast cancer patients.Methods Choose patients by random with a questionnaire for malignant breast cancer patients(Chinese edition),and analyze the influencing factors in a multiple linear regression.Results The total extent of information needs among the patients is 65.615±20.854,with much stronger needs among those with younger age,higher income and/or suitable for surgery.Conclusion Information needs of the patients were found to depend on their various social attributes.Based on this founding,recommendations were made in terms of contents of information needs,care for vulnerable groups,and channels of information communication.

To cement the 5 years’ staged achievement of new medical reform so as finally achieve the objective of medical reform, public hospital would become the aim of reform in the following stage. On one hand , canceling “using drug to supple health care” in public hospitals has came into the crucial stage; on the other hand, the government needed to increase financial input and ensure the implementation of security fund. What was the relation between these two reforms? Therefore , based on the survey of county-level public hospitals in G Province, relationship among government compensation method, hospital public profit operation mechanism and profit-driven operation mechanism were analyzed; besides, different financial insurance mode had different operation efficiency. Because the construction of hospital expense under public profit operation mechanism was more reasonable , the required government insurance fund was less, which only accounted 32.9%of the required fund under the profit-driven operation mechanism. Therefore, the policy suggestions that government need to “buy the mechanism” and “be brave to implement ‘subtraction reform’” were proposed.

ObjectiveTo introduce a new procedure of penile lengthening which yields satisfactory outcomes and avoids persistent postoperative hydrophallus with the results of fresh body researches.MethodsIndirect intralymphatic injection and routine H-E staining were used to investigate the ramification of lymphatics of penis on fresh bodies.We found that the lymphatic return of penis was primarily mediated by dorsal and bilateral superficial lymph vessels,which converged dorsally at the root of penis and then ramified into the superior region of pubic symphysis and bilateral inguinal grooves.However,most previous incisions were done at the converging region of lymph vessels at the dorsal root of penis.It blocked most lymphatic return.Our modified method avoided interruption of lymph vessels in the converging region.Based on the results of the above investigations,the operative incision at the root of penis was shifted upward by 1.5 cm,and then the lengthened cavernous body of penis was covered by scrotal flap.ResultsEighty patients who accepted the new method achieved satisfactory outcomes.Postoperative hydrophallous decreased gradually from the 3rd day and vanished basically on the 6-7 day.ConclusionsOur modified procedure of penile lengthening reduces the damage of these vessels.Consequently,postoperative hydrophallus resolves fast and the new procedure yields high success rates,sound appearance and sensation of penis.Therefore,the new procedure is an ideal option for surgenons in clinical practice.

AIM: To identify the changes in microRNA ( miRNA ) profile of human lens epithelium ( HLE) induced by H2 O2 and the role of miRNA in oxidative stress induced apoptosis.
METHODS: HLE cell line HLE - B3 was treated by 100μmol/L H2 O2 for 24h and the total RNA were isolated by Trizol reagent. miRNA profile was generated by miRCURYTM LNA microRNA Array. The target genes of differentially expressed miRNAs were predicted by bioinformatics software.
RESULTS:Twenty-eight miRNAs showed significantly differential expression after H2 O2 treatment, 18 miRNAs upregulated and 10 miRNAs downregulated. The differentially expressed miRNAs may involve in apoptosis of lens epithetium and development of cataract through targeting BCL2L2 and MIP.
CONCLUSION: H2 O2 can induce dramatically changes in miRNA profile of HLE, which may play a pivotal role in the pathogenesis and development of cataract.

Objective To observe the effects of flunarizine on the intracerebral hemorrhage (ICH) of Wistar rats at different time points. Methods An ICH rat model was established by collagenase. The concentrations of intracellular free Ca 2+ at different time points after ICH were determined by the fluorescence detector with an indicator, Fura 2/AM. The effects of flunarizine on the concentrations at different time points were also observed. Results The concentration of intracellular free Ca 2+ began to increase at 0.5 h, increased significantly at 6 h, and peaked at 24 h (about 4 folds as high as that before hemorrhage), but began to decrease at 72 h after acute intracerebral hemorrhage. Flunarizine decreased the concentration of intracellular free Ca 2+ significantly ( P

After the practical experiences were described in cultivating military aviation medical information research brand, providing selective dissemination of information in novelty assessment of military aviation medical literature , establishing the platform for pushing the aviation medical information , integrating the aviation medical resources , and carrying on the methodology research of aviation medical information, the successful results in reference service were summarized in novelty assessment of military aviation medical literature.

Objective To investigate the risk factors for occurring cerebral microbleeds( CMBs)in patients with ischemic stroke Methods A total of 184 consecutive patients with ischemic stroke admitted from January 2012 to January 2014 in General Hospital of Beijing Military Command were enrolled prospec-tively. They were divided into either a CMB group( n=60 )or a non-CMB group( n=124 )according to whether head MRI magnetic sensitive weighted imaging( SWI)revealed CMBs or not. The general clinical data and serum biochemical results of the patients in both groups were collected. Renal creatinine clearance ( Ccr)was calculated,and the data of both groups were analyzed by the univariate and multivariate analyses. Results (1)The results of univariate analysis showed that there were significant differences in age(63 ± 10 vt. 58 ± 10 ),male( 65. 0%[ 39/60 ]vs. 49. 2%[ 61/124 ]),the incidence of leukoaraiosis( LA ) (73. 3%[44 cases]vs. 47. 6%[59 cases]),and serum homocysteine(Hcy)levels(20 ± 8 vs. 16 ± 6μmol/L)between the CMB group and the non-CMB group(P<0. 05). The Ccr level(89 ± 10 mL/(min·1.73m2)of the CMB group was lower than(106 ±6mL/(min·1.73m2)of the non-CMB group. There was significant difference(P <0. 05).(2)Multivariate logistic regression analysis showed that age(OR,1. 103,95%CI1. 024—1. 989,P =0. 028),LA(OR,3. 121,95%CI 1. 310—7. 436;P <0. 05)and impaired kidney function(OR,1. 890,95%CI 1. 358—3. 076;P<0. 01)were the independent risk factors for the occurrence of CMBs in patients with ischemic stroke. Conclusion Age,LA and impaired kidney function are the independent risk factors for occurring CMBs in patients with ischemic stroke.

Objective To investigate the relationship between retinal vascular lesions and cerebral microbleeds ( CMBs) . Methods One hundred twenty-three consecutive patients with cerebral infarction who could sit and finish fundus photography at the Department of Neurology, General Hospital of Beijing Military Command from August 2013 to August 2014 were enrolled prospectively. After informed consent, the patients underwent head MRI examination and bilateral eye fundus photography. They were divided into either a cerebral microbleed group ( CMB group, n =52 ) or a non-cerebral microbleed group ( nCMB group,n=71) according to whether they had CMBs or not. The general clinical data and retinal vascular lesions ( microaneurysms,hard exudates,cotton wool spots,bleeding spots,focal retinal arteriolar narrowing, arteriovenous cross sign,and silver thread-like changes) of both groups were compared. Results (1)There were significant differences in age,hypertension,diabetes,systolic blood pressure,diastolic blood pressure, fasting blood-glucose,triglyceride,and C reaction protein between the CMB group and the non-CMB group (all P<0. 05). (2) The incidences of hard exudates,focal retinal arteriolar narrowing,and arteriovenous cross sign of patients with CMB were higher than those with non-CMB,and there were significant differences (χ2 =6.233,7.675,and9.544,respectively;P=0.040,0.031,and0.019,respectively).(3)There were significant differences in the incidences of hard exudates,focal retinal arteriolar narrowing,and arteriovenous cross sign in patients with different severity of CMBs (Z= -2. 317,-2. 294 and -2. 157,respectively;P=0. 029,0. 033,and 0. 039,respectively). In patients with severe CMB,there were more patients with hard exudates,focal retinal arteriolar narrowing,and arteriovenous cross sign. (4) The age (OR,3. 623, 95%CI 2. 631-7. 866),hypertension (OR,4. 348,95%CI 3. 734-10. 563),diabetes (OR,3. 831,95%CI 2.126-9. 245),fasting blood-glucose (OR,3. 329,95%CI 2. 631-11. 012),arteriovenous cross sign (OR,5. 437,95%CI 3. 441-13. 606),and hard exudates (OR,4. 054,95%CI 3. 137-13. 252) were the independent risk factors for the occurrence of CMBs. Conclusion The arteriovenous cross sign, focal retinal artery narrowing,and hard exudates in retinal vascular lesions are associated with the CMBs and different degree of CMBs classification. Both the arteriovenous cross sign and hard exudates are the independent risk factors for CMBs.

Acute myeloid leukemia (AML) is a group of heterogeneous diseases with diverse genetic abnormalities,variable responsiveness to therapy and prognosis.In recent years,a lot of information has become available regarding chromosome and gene mutations that occur in AML and their influence on prognosis.Improvements in the understanding of molecular biology of AML are critical for accurate diagnosis.risk stratification,monitoring of minimal residual disease and provides opportunities to develop targeted therapies and improve the clinical outcome.This article reviewed chromosome abnormalities and characteristic gene mutations,and discussed their clinical signiflcances and presented new drugs in clinical trials presented in the 55th ASH annual meeting.