There are many ways to treat pathological scars.Silicon gel sheeting as a noninvasive and convenient method, are widely used in clinic.The article reviews the lituratures on theoretical research of silicon gel sheeting and its efficacy in the past 26 years, and analyzes the effectiveness of silicon gel sheeting for comparison with other methods.

OBJECTIVE:To investigate the effects of gemcitabine combined with radiotherapy and chemotherapy on angiogen-esis and cell invasion ability of cervical cancer. METHODS:Totally 37 patients undergoing radical operation selected from Chongq-ing Kaizhou District People's Hospital during Jan. 2015-Jun. 2016 were divided into observation group(n=19)and control group (n=18). Control group was given 3-D conformal radiation and chemotherapy which included Cisplatin injection 70 mg/m2,ivgtt+Fluorouracil injection 600 mg/m2,ivgtt on 1st and 21st day after radiotherapy. Observation group was additionally given Gemcitabi-ne hydrochloride for injection 800 mg/m2,ivgtt,1st and 8th day after radiotherapy. A treatment course lasted for 28 d. The radical hysterectomy and pelvic lymphadenectomy were conducted after 2 courses of treatment. Clinical efficacy,microvessel density (MVD) of tumor,vascular endothelial growth factor (VEGF),Wnt1,Wnt3a,Wnt8 and β-catenin level were observed in 2 groups. The relationship of MVD with VEGF was analyzed,and the occurrence of ADR was recorded. RESULTS:The total re-sponse rate of observation group(84.2%)was significantly higher than control group,with statistical significance(P<0.05). The levels of MVD,VEGF,Wnt1,Wnt3a,Wnt8 and β-catenin in 2 groups were significantly higher than healthy tissue;above index-es of focus tissue in observation group were significantly lower than control group,with statistical significance (P<0.05). MVD was positively correlated with VEGF. There was no statistical significance in the incidence of ADR between 2 groups (31.6% vs. 27.8%,P<0.05). CONCLUSIONS:Gemcitabine combined with radiotherapy and chemotherapy can regulate the angiogenesis of cervical cancer cell and the activity of Wnt/β-catenin signal pathway,and reduce the invasion ability of cervical cancer cell,which may be the important mechanism of anti-tumor effect with good safety.

BACKGROUND:At present,new drug and dosage form to treat diabetes mellitus has emerged continually,but it has toxic reaction,side effects,and contraindication or unindication.Especially that it cannot prevent the natural proceeding of diabetes mellitus from aggravation.Based on theory and experience of traditional Chinese medicine,and combining modern methods of pharmacology and pharmaceutical chemistry,it has become one of the hotspots in researches on diabetes mellitus at present to investigate and empolder preparation of pure traditional Chinese medicine that has good effect and little toxicity.OBJECTIVE:To investigate the pharmacological effect of Chinese compound of Qiangyi Jiangtang capsules on diabetic rat models induced by high-fat forage and streptozotocin.DESIGN:A comparative observation of animal trial.SETTING:Staff Room of Pharmacology,Yunnan College of Traditional Chinese Medicine.MATERIALS:Totally 100 2-month-old Wistar rats of common degree,weighing(180±25)g were used.There were 50 males and 50 females,14 rats were randomly selected as the blank control group,and the other 84 rats were used in model establishment.The treatments of the animals in the experiment were accorded with the ethical standard.METHODS:The experiment was carried out in the Staff Room of Pharmacology,Yunnan College of Traditional Chinese Medicine from March to April in 2004.Diabetic models were induced by high-fat forage and streptozotocin.The successful model rats (n=54) were randomly divided into the model group,positive control group and the high,middle and low dosage of Qiangyi Jiangtang capsules groups.The dosages in the low,middle and high dosages treated groups were 0.4,1.2 and 2.0 g/kg respectively. The rats in the blank control group and model group were given isovolume of saline,and those in the positive control group were administrated intragastrically with metformin (0.5 g/kg).After administration,blood glucose,glycosylated hemoglobin and serum fructosamine were determined at 1 month,and the amounts of water drinking and food intake were determined at the 2nd and 4th weeks.MAIN OUTCOME MEASURES:Results of blood glucose,glycosylated hemoglobin and serum fructosamine;amounts of water drinking and food intake.RESULTS:Totally 100 Wistar rats were used,32 of them were excluded due to failure in the model establishment,and the other 68 were involved in the final analysis of results.The blood glucose,glycosylated hemoglobin and serum fructosamine in the high,middle and low dosages of Qiangyi Jiangtang capsules groups were all significantly lower than those in the model group(P＜0.01).The amounts of water drinking at the 2nd and 4th weeks in the high,middle and low dosages of Qiangyi Jiangtang capsules groups were significantly lower than those in the model group (P＜0.01),and the amounts of food intake were also lower than those in the model group (P＜0.05 or 0.01).CONCLUSION: Qiangyi Jiangtang capsules could remarkably decrease the levels of blood glucose, glycosylated hemoglobin and serum fructosamine,and the amounts of water drinking and food intake of rat models of diabetes mellitus.It was proved that traditional Chinese medicine for diabetes mellitus is worthy of further development and research.

Objective:To establish an HPLC method to determine the content of rosmarinic acid in Wuling capsules. Methods:The chromatographic column was Linksil-ODS (250 mm × 4. 6 mm,5 μm) with a gradient elution mode at the flow rate of 0. 8 ml· min-1,and the mobile phase was acetonitrile-water (32∶68) including 0. 5% acetic acid. The detection wavelength was 319 nm, the column temperature was 25 ℃ and the injection volume was 20 μl. Results: The linear range was 2-80 μg·ml-1 and the standard curve showed good linear regression within the range(r=0. 999 8). And the average recovery was 97. 07﹪ (n=6) with the inter-day precision (RSD) of 1. 35 %. Conclusion:The method is accurate and rapid with good reproducibility in the determination of ros-marinic acid in Wuling capsules, which can be used to control the quality of rosmarinic acid in Wuling capsules.

Glioma ; pathology ; Humans ; Neoplastic Stem Cells ; pathology

objective To describe the clinical features of patients with posterior circulation ischemic stroke.Methods 216 patients with posterior circulation ischemic stroke admitted in our department during 2004-2006 were analyzed retrospectively.All patients were undertaken MRI on admission and responsble lesions were identified at the posterior circulation territories.The patients'clinical symptoms and signs were evaluated and the relationships between lesion locations and clinical characteristics were analyzed.Results The common symptoms of posterior circulation ischemic stroke were unilateral limb Weakness(81.9%),speech difficulty(46.3%),dizziness(33.8%),and unilateral limb numbness (31.O%).The common signs of posterior circulation ischemic stroke were unilateral limb weakness (81.9%),central facial or lingual palsy(61.1%),dysarthria(46.3%),unilateral limb sensory loss (31.0%),and ataxia(30.1%).The incidence of crossed paralysis was low(2.8%).Isolated vertigo was rare (1.4%).Predominant clinical features such as bulbar paralysis,unconsciousness,visual disorder and amnesia can help to localize the lesions.Typical brainstem syndromes had topographic meanings.Conclusions The clinical features of patients with posterior circulation ischemic stroke were complex.Predominant symptoms can help to diagnose the posterior circulation ischemic stroke.

Objective:Since malignant fibrous histiocytoma (MFH) may be taken as an undifferentia-ted pleomorphic sarcoma (UPS), this study was conducted to reassess 33 previously diagnosed MFH cases in the past 10 years based on the latest WHO concept. And then to search for the clinicopathological features, probably tumorigenesis, and the line of differentiation of the remaining MFH/UPS cases.Methods: Thirty-three cases in tissue microarray were studied by immunohistochemistry with panels of neurogenic, myogenic, and lipogenic antibodies. Three expertise pathologists reevaluated the slides separately. Results: Among the 33 cases, 17 cases (51.5%) of MFH had their diagnoses changed, including 5 leiomyosarcomas, 3 malignant peripheral nerve sheath tumors, 1 fibrosarcoma, 1 inflammatory myofibrosarcoma, 1 giant cell tumor and 1 angiomatoid fibrous histiocytoma. The remaining 16 cases (48.5%) were finally diagnosed as MFH/UPS, among which patients were mainly old adults (median age: 63 years; range: 38 to 76 years). The median tumor size was 6.0 cm (range: 3.0 to 14.0 cm), 8 cases (50%) located in lower limb and 5 cases (31.3%) located in thigh. These tumors had marked cytological and nuclear pleomorphism. Immunohistochemistry showed that Vimentin was strongly positive in all 16 MFH/UPS (100%), Muscle-specific actin was variously positive in 8 cases (50%) and 1 case focally expressed Desmin. Eleven cases (68.8%) variously expressed CD68 (KP1) and 7 cases (43.8%) expressed CD68 (PG-M1), which were much higher than leiomyosarcoma, malignant peripheral nerve sheath tumor and liposarcoma with significant difference. Moreover, Ki67 expression rates were from 10% to 100%, including 14 cases more than 50% and 11 cases more than 70%. However, only 2 cases (12.5%) showed P53 positive. Conclusion: MFH/UPS often show marked histological pleomorphism, and the diagnosis must be made by exclusion of other definitive sarcomas, especially myogenic and neurogenic sarcoma. Only Vimentin was always expressed in MFH/UPS, while some of the tumors were positive for myogenic antigen and CD68. It was suggested that MFH/UPS might arise from primary mesenchymal cells, and some cases exhibited fibroblastic and/or myofibroblastic features. In addition, histiocytic phenotypic marker did have more expression in MFH/UPS than in other sarcomas. MFH/UPS still had certain clinicopathological characteristics.

Objective: To investigate whether the plasmid ?1neo-hgp100 could be expressed and presented in vitro and could protect the immunized mice from B16F10 challenge in vivo. Methods: ?1neo-hgp100 plasmid was constructed in which the DNA sequence encoding hgp100 CTL epitope inserted into CDR3 of ?1-neo vector. The expression of anti-bodized antigen and IFN-? in supernatant was measured by ELISA respectively after transfection J558L with ?1neo-hgp100 and further co-culture of J588L transfacted with ?1 neo-hgp100 and pmel TCR transgenic T cell. After introspleenic inoculation of ?1neo-hgp100, the protective efficacy of the gene vaccine was observed by means of measuring the tumor area every two days. Results: ?1neo-hgp100 could be expressed and presented in vitro, the immunogenecity of CTL epitope of hgp100 was strong enough and could activate gp100 specific T cell, the mice immunized with the gene vaccine could resist the tumor challenging in vivo. The mean survival time was prolonged to 36 days, compared to control group (P

Objective To investigate the polymorphism of glutathione S-transferease P1 gene(GSTP1) and the association between the mutation and susceptibility in childhood asthma.Methods The distribute frequency of Ile105/Ile105,Ile105/Val105 and Val105/Val105 ge-notype in GSTP1 of 51 children with asthmatic and 40 normal children were studied with polymerase chain reaction-restriction tragment length polymorphism(PCR-RFLP).Results The frequencies of Ile/Ile Ile/Val,Val/Val genotype were 66.7%,27.4% and 5.9%,the frequencies of Ile,Val allele were 80.4% and 19.6% in the asthmatic group.But the frequencies of Ile/Ile,Ile/Val,Val/Val genotype were 90.0%,7.5% and 2.5%,the frequencies of Ile,Val allele were 93.8%,6.2% in control group.The frequencies Ile/Val,Val/Val genotype and Val allele in asthmatic group were more than that in control group.A significant difference was found in the frequency distribution of GSTP1 genotypes between two groups(?2=6.947 P

Objective To investigate the regularity of chronopharmacology of bruceolic oil emulsion ( BOE) in treating H22 tumor-bearing mice. Methods The KM mice model bearing H22 hepatocellular carcinoma was established. And then the model mice were divided into groups according to the condition of illumination and medicine intervention. After treatment for 10 days, the tumor-growth inhibitory rate, viscera indexes, and hepatic and renal function indexes were observed. Results Tumor mass and ratio of tumor mass to body mass were decreased in medication groups under the normal and disordered illumination. The tumor mass and ratio in mice treated with BOE before dark phase of normal illumination and disordered illumination, and during the dark phase of disordered illumination differed from those of the model group at the corresponding illumination phase (P<0.05) . Under normal illumination, BOE-treated mice at 10 pm of dark phase had lower liver index but higher thymus index than the model group (P < 0.05) . Under disordered illumination, BOE-treated mice at 7 am of dark phase had higher spleen index than the model group ( P < 0.05) . Conclusion BOE exerts certain inhibition of H22 hepatocellular carcinoma under both normal illumination and disordered illumination, and the effect will be better when medication is given in dark phase period.