Objective To observe the therapeutic effect of Kangliyin(KLY,the compound Chinese herbal formulation with the functions of clearing heat and damp elimination,regulating stomach and dissipate phlegm) on the models with infection of infectious bronchitis virus(IBV),and to evaluate its effect on the IBV N gene relative expression by using real-time florescent quantitative polymerase chain reaction(PCR).Methods Seven-day-old chicks were inoculated with IBV by the nasal and ocular administration.The chicks were divided into 6 groups by stratified random sampling according to the body weight.The 6 groups were blank control group,model group,three KLY treatment groups(receiving KLY at the dosage of 10 g/kg? d-1,20 g/kg? d-1 or 40 g/kg? d-1,respectively),and positive control group(ganciclovir at the dosage of 0.004 g/kg? d-1).The chicks in the model group and blank control group received normal saline,and the medication groups received the corresponding medicine according to the experimental design from the next day of inoculation,and the treatment lasted 5 days.The relative quantity of IBV N gene expression in the lung and bronchial tissues were assayed by real-time florescent quantitative PCR,and the clinical manifestations and the pathological changes of all groups were also examined.Results Compared with the model group,KLY could significantly inhibit the IBV N gene expression,improve the general clinical symptoms and respiratory symptoms,and improve the pathological changes in the bronchus and lung of the IBV infected model.Conclusion KLY could effectively inhibit the viral replication,repair the pathological damage in the bronchus and lung,and improve the general clinical symptoms in the IBV infected model,which is beneficial to the recovery of the chicks from IBV infection.

After a description of the concept of accumulative point system and studies on its theories and practice in libraries, it was pointed out that introduction of accumulative point system into hospital library could improve the utilization of resources, normalize the borrowing behaviors of readers, creating hormonal and fair borrowing environ-ments, and certain measures were put forward for implementing accumulative point system in hospital library.

Objective To compare the clinical effect of three types of intrauterine devices and their adverse events.Methods From June 2012 to June 2013,683 women of reproductive age underwent the insertion of Gyne Fix-IN IUD,Mcu IUD and HCu280 IUD in Family -Planning Service Outlet of Yi -shan District in Zaozhuang City were collected in this study.The patients were followed up for 1year.The clinical effect and adverse events of three types of intrauterine devices were compared.Results The expulsion rate of Gyne FixIN IUD (0.88%)group was lower than those in the Mcu IUD (3.95%)and HCu280 IUD (4.41%)group (χ2 =4.565,5.514,all P <0.05).And the removal rate for medical reasons of Mcu IUD group (5.26%)was higher than those in the FixIN IUD(1.75%)and HCu280 IUD (1.32%)group (χ2 =4.145,5.543,all P <0.05).The continuation rate of Mcu IUD (90.35%) group was higher than those in the FixIN IUD (96.93%)and HCu280 IUD(95.59%)group (χ2 =8.286,4.784, all P <0.05).The pregnancy rate of three groups were no significant difference (P >0.05).Conclusion The three kinds of IUDs could be used as the first menstrual period returns after vaginal delivery.HCu280 IUD has good clinical effect.The effective contraception rate is high while adverse events are low.It is worthy of clinical use.

Objective To study the effects of nilestriol taken orally before removing the intrauterine device (IUD) in postmenopausal women.Methods Women in group one took nilestriol 4rag orally once a week before op- eration;women in another group took no medicine.Two groups women were removed IUD by routine operation.Re- suits 100% of the IUD were removed successfully for women in group one,85% of the IUD were removed for women in another group,and there were significant differences (P

OBJECTIVE:To establish a method for uncertainty analysis about the content of norethisterone and its content uniformity in HPLC determination of norethisterone tablets. METHODS: The mathematic model of uncertainty measurement was deduced in the procedure of determining the content and content uniformity in norethisterone tablets by HPLC, and the factors influencing the uncertainty were determined and the factors were evaluated. RESULTS: The combined uncertainty of both content and content uniformity were 1.2%; the extended uncertainty of both were 2.4%, and the determined content were (101.8?2.4)% and (102.3?2.4)% respectively. CONCLUSIONS: This method is applicable for the uncertainty analysis about the content and content uniformity of norethisterone tablets.

AIM:To study the effects of Kangliyin compound (KLY,the compound formulation of a Chinese herbal medicine has 15 kinds of ingredients,including artemisia apiacea,baical skullcap root,tangerine peel,pinellia tuber,frutus aurantii immaturus,caulis bambusae in taeniis,tuckahoe,the larva of silkworm with botrytis,turmeric,agastache,rhubarb,cicada ecdysis,and so on.) treatment on the infectious bronchitis virus (IBV) infection models and to evaluate its effects on the levels of IBV antibody in the serum. METHODS:Seven-day-old chicks were inoculated with the respiration type of IBV M41 strain. All the chicks were bred in a low temperature and highly moisturized controlled environment. The model group and normal control group were fed with sterile normal saline,while the three treatment groups were administrated with KLY compound. Positive control group was irrigated with ganciclovir. These feeds were lasted for 5 d. The clinical symptoms and the pathological changes of all groups' bronchus and lung tissues were examined. The levels of IBV antibody in the serum in each group were also checked by using ELISA method at the end of the study. RESULTS:Compared with the model group,KLY compound significantly increased the serum titer of IBV antibody in the three treatment groups (P

Objective To investigate the clinical efficacy of kidney-tonifying and blood-activating therapy on puberty polycystic ovarian syndrome(PCOS).Methods Thirty-one PCOS patients were randomly allocated into 2 groups:16 patients(TCM group)received kidney-tonifying and blood-activating Fuke No.5 Prescription combined with herbal medicine based on the menstrual cycle,and 15 patients(WM group)received oral administration of western medicine of diane-35.Changes of clinical symptoms and signs,B-ultrasonic results and serum hormones levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),prolactin(PRL),testosterone(T)and estrogen(E2)were examined before treatment,after treatment for 3 menstrual cycles and on the 3rd cycle after suspension.Results The menstuation and ovulation,serum sexual hormones(except FSH and E2)levels,and B-ultrasonic indexes(except the volume of uterus)were obviously improved in the two groups after treatment for 3 menstrual cycles(P0.05 compared with those before treatment),but remained at the levels after treatment for 3 menstrual cycles(P

Teaching evaluation from the students is the necessary key point in the management process of teaching quality in colleges. This essay introduces the design and development of the system of teaching evaluation on net in colleges about the technology of data base of ASP NET and JAVA. This system will overcome the deficiency of the traditional evaluation model in teaching,which remains the short comings of too large a number of data,long time in data processing,as well as the low working efficiency. It also probes on the application of the students’ evaluation system.

The potassium content in serum, red cells andsome factors involved in hypopotassium were ob-served in 30 cases with open heart surgery. Ac-cording to the results, we conclude that (1) Hy-popotassium tendency before and during cardiopul-monary bypass was mainly produced by the potas-sium moving into the red cells from the serum. Af-ter Cardiopulmonary bypass it was mainly owing tothe excretion of potassium through the urine. (2)The potassium shifting into the red cells waschiefly affeced by the bypothermia, pH, and perfu-sion time. (3) The red cell potassium lost whenplasma potassium value set below 4. 0 m mol/L.1. 56 m mol/kg of potassium administered was atleaset reqired to maintain plasma potassium leverover 4. 0 m mol/L.

Objective:To observe therapeutic effect of aerobic exercise on blood pressure and vasoactive mediators in patients with isolated diastolic hypertension (IDH).Methods:A total of 93 recently diagnosed IDH patients were se- lected and randomly divided into exercise intervention group (n=47,received aerobic exercise,twice/d,50~80 min/time for six months)and control group (n=46,didn't receive aerobic exercise).Office blood pressure (OBP), ambulatory blood pressure monitoring (ABPM)and levels of vasoactive mediators were measured and compared be- tween two groups before and six months after intervention.Results:There were no significant difference in levels of OBP,ABPM indexes and related vasoactive mediators between two groups before intervention (P>0.05 all).Com- pared with before intervention,after six-month intervention,there were significant reductions in levels of office di-astolic blood pressure (ODBP),mean DBP (mDBP),daytime DBP (dDBP)and nighttime DBP (nDBP)in both groups (P<0.05 or <0.01);compared with control group,there were significant reductions in levels of ODBP [(89.23±14.70)mmHg vs.(79.26±13.18)mmHg],mDBP [(86.51±14.30)mmHg vs.(77.20±12.45)mm- Hg],dDBP [(87.93±12.16)mmHg vs.(78.02±11.27)mmHg]and nDBP [(83.18±12.57)mmHg vs.(74.22 ±11.36)mmHg]in exercise intervention group,P<0.05 all.Compared with control group,after six-month inter- vention,there were significant rise in levels of adrenomedulin (AM)[(36.75±5.13)ng/L vs.(47.59±6.82)ng/L]and prostaglandinI2 (PGI2)[(89.55±16.31)ng/L vs.(99.24±18.20)ng/L],and significant reductions in levels of angiotensin Ⅱ (AngⅡ)[(98.30± 15.65)ng/L vs.(91.28±17.40)ng/L]and thromboxane A2 (TXA2 )[(93.61±15.27)ng/L vs.(80.24±12.31)ng/L]in exercise intervention group,P<0.05 all.Conclusion:Aer- obic exercise can significantly lower blood pressure and correct vasoactive mediator level disorder in IDH patients.