In the teaching reform of cytobiology,we should first formulate a reasonable syllabus according to training goals,pay attention to course content reform,strengthen connection with other preclinical medicine subjects,combine quality-oriented education in class instruction,and cultivate students scientific and original spirit.The experimental courses should be offered from higher level and be conformed with subject development.The teacher should have innovative thinking,adopt diversified teaching methods,and fully arouse students' study enthusiasm.

Objective To probe into the application feature and use tendency of traditional Chinese medicine (TCM) injections in our hospital;To evaluate rational drug use and provide reference for related researches. Methods TCM injections in our hospital during 2010-2012 were analyzed by employing the analytic method of defined daily dose, with a purpose to evaluate frequency of drug use and rationality of medication expenses. Results From 2010 to 2012, drug possession rate of TCM injections in our hospital was 4.07%-4.47%;the amount of medication accounted for 10.50%to 12.69%of the amount of all drugs;the annual growth rate increased significantly, among which TCM injections used for removing blood stasis took the lead;frequency of drug use rose annually;medication synchrony in 3 years indicated that daily expensesof Shenkang Injection, Aidi Injection, salvianolateinjection, safflor yellow sodium chloride injection and other TCM injections were on the high side. Conclusion From 2010 to 2012, TCM injections in the clinical application in our hospital were of relatively high frequency, and the TCM injections used for removing blood stasis took the lead. Daily expenses of TCM injections drugs were on the high side and there may be unreasonable application.

A new method of the separation of hydrocarbons and glycerides in liver oil of Mustelus jriseas was designed. The Waters Prep-500A Liquid Chromatograph with silica gel column and differential refractive index detector was used. The mobile phase was petroleum ether and ethyl acetate. The pure hydrocarbons' and glycerides were obtained from the liver oil.

Objective To investigate the relationship between the serum concentration of amiodarone and QT dispersion.Methods The serum concentration of Am and DEA were determined by HPLC during the treatment in forty Patients to study the relationship between the serum concentration and QTd.Results After using amiodarone,QT interval was significantly increased[(383±31)ms vs(418±29)ms,P＜0.05].In the same time QTd was significantly decreased[(34±12)ms vs(21±9)ms,P＜0.05].With maintaining dosage,the serum concentration of DEA had positive relationship with the increment of QT(r=0.50,P＜0.05).While with loading dosage,the serum concentration of amiodarone had no relationship with it.The serum concentration of amiodarone had no relationshiP with the decrement of QTd in all patients.And it varied in a large range.Between the groups of different therapeutic effect,the serum concentration had no statistical difference.Two patients appeared the adverse effects.Their serum concentration of amiodarone weren't higher than the average concentration of the group.Conclusion The serum concentration of amiodarone couldn't be an index tO estimate the effect and the occurrence of adverse effects.

Tripterygium Hypoglaucum Hutch and Tripterygium Wilfordii Hook.f are all Evonymus alata family Tripterygium Wilfordii genus plants.They are generally used in the clinical treatment in department of dermatology.They resemble each other not only in some chemical constitutions,but also in some pharmacologic actions(such as anti-inflammatory,anti-tumor,immune suppression,antifertility and so on),but they are all poisonous plants.When they are used for therapy,they can induce some toxic reactions,even the death of patients.In order to facilitate the adoption of the two drugs,we review their pharmacologic actions.

Magnetic polymer microspheres are a novel functional material that has been developed recently.This kind of organic-inorganic nanoparticles composites can lead to many reactive functional groups on the surface of microspheres by means of copolymerization and surface modification.Moreover,this kind of materials is strongly response to the magnetic field.As the carrier of enzyme,cell and drug,it can be widely applied in many fields,such as bio-engineering,cytology,biomedical science and so on.The recent research progresses of magnetic polymer microspheres are reviewed.The preparation and characterization of magnetic microspheres are also introduced.Immobilization of enzyme onto magnetic polymer microspheres is emphasized and every method of immobilized enzyme is compared and analyzed.The prospects of magnetic polymer microsphere are also proposed.

This paper is to prepare curcumin (Cur) loaded mesoporous silica nanoparticle (Cur-MSN), evaluate its release behavior and anti-cancer activity in vitro. Mesoporous silica nanoparticle (MSN) was prepared by polymerization method and Cur-MSN was obtained using solvent evaporation method and impregnation centrifugation method. The preparation method was optimized using entrapment efficiency (EE) and loading efficiency (LE) as indexes. Cur-MSN was characterized with scanning electron microscope and its particle size and zeta potential were determined. Finally, in vitro release behavior in 0.2% SDS solution and its cell-killing effect on HeLa cells were also evaluated. The Cur-MSN prepared with process optimization method was round and uniform and exhibited typical mesoporous characterization. The mean particle size and Zeta potential of Cur-MSN were 75.8 nm and -30.1 mV, respectively. EE and LE of three batches of Cur-MSN were (72.55 ± 2.01)% and (16.21 ± 1.12)%, respectively. In vitro release behavior of Cur-MSN showed a sustained release profile with 83.5% cumulative release within 96 h. The killing effect of Cur-MSN on HeLa cells was dose-dependent with IC50 of 19.40 mg x L(-1), which was similar to that of Cur.
Antineoplastic Agents, Phytogenic ; chemistry ; pharmacology ; Curcumin ; chemistry ; pharmacology ; Drug Carriers ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Nanoparticles ; chemistry ; Neoplasms ; drug therapy ; Particle Size ; Porosity ; Silicon Dioxide ; chemistry

Acute leukemia is a common malignancy tumor in children. Hemorrhage is one of the common symptoms and causes of death. The abnormality of platelet count, quality and function can cause bleeding. The understanding of platelet function and platelet parameters can provide an important clinical information for children with acute leukemia in evaluating the effect of treatment,the function of bone mar-row and the prevention of bleeding and so on.

Functional dyspepsia( FD)is a common functional gastrointestinal disorder,its pathogenesis has not been fully clarified. Recent studies showed that gastrointestinal mucosal low-grade inflammation had been found in patients with FD,and impaired mucosal integrity,psychological factor and abnormal brain-gut axis may promote the inflammation formation,which may also contribute to the onset and persistence of FD. This article reviewed the advances in study on gastrointestinal mucosal low-grade inflammation in FD.

Objective To evaluate the therapeutic efficacy of the comprehensive auricular point therapy in treating post-stroke urinary incontinence. Method A hundred patients with post-stoke urinary incontinence were randomized into a treatment group and a control group, 50 in each group. The treatment group was intervened by comprehensive auricular point therapy, while the control group was by rehabilitation training. The therapeutic efficacies were evaluated after 2 treatment courses. Result The total effective rate was 82.0%in the treatment group versus 62.0%in the control group, and the difference was statistically insignificant (P>0.05). Conclusion Comprehensive auricular point therapy is effective in treating post-stroke urinary incontinence.