Based on the research of risk assessment of medical equipment safety and operation to formulate evaluation criteria and methods of how to use the practical management work. Objective to evaluate the practical method is to use and the risk of adverse events of medical equipment in operation risk in order to find the risk, control risk, ensure safety in time, so that Improve the level of risk management of medical equipment.
Equipment Safety ; Humans ; Risk Assessment ; Risk Management

OBJECTIVETo compare the efficacy differences between needle-knife therapy and acupuncture-cupping for treatment of cervical spondylosis (CS) of cervical type.

METHODSSixty cases of CS were randomly divided into a needle-knife group (30 cases) and an acupuncture-cupping group (30 cases). The needle-knife therapy was applied at points among superior nuchal line of occipital bone, bilateral neck muscle, neck centerline, trapezius and medial border scapula for only once. In the acupuncture-cupping group, acupuncture was applied at Fengchi (GB 20), Fengfu (GV 16), Tianzhu (BL 10), Dazhui (GV 14), Jianjing (GB 21), Jiaji (Ex-B2, from C4 to C6), Houxi (SI 3) and Ashi point, followed by cupping on local skin, once every other day for totally six times. The score of neck stiffness and visual analogue scale (VAS) were observed before and after treatment, in follow-up of 1, 3 and 6 months after treatment in the two groups, and the efficacy was compared.

RESULTSIn the needle-knife group, 9 cases were cured, 12 cases were markedly effective, 8 cases were effective and 1 case was failed; the total effective rate was 96.7% (29/30) and the cured and markedly effective rate was 70.0% (21/30). In the acupuncture-cupping group, 8 cases were cured, 9 cases were markedly effective, 11 cases were effective and 2 cases were failed; the total effective rate was 93.3% (28/30) and the cured and markedly effective rate was 56.7% (17/30). The difference of total effective rate in the two groups was not statistically significant (P > 0.05), but the cured and markedly effective rate of needle-knife group was significantly superior to that of acupuncture-cupping group (P < 0.05). The needle-knife therapy was significantly superior to acupuncture-cupping on improvement of neck stiffness in the follow-up of 1, 3, 6 months after treatment (P < 0.05, P < 0.001); both treatments were effective on relief of neck pain, but the needle-knife group had better effects in the follow-up of 3 and 6 months after treatment compared with acupuncture-cupping group (both P < 0.01).

CONCLUSIONThe needle-knife therapy has better effects on neck stiffness and pain relief than acupuncture-cupping, and it is more treatment time saving.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Cervical Vertebrae ; physiopathology ; Female ; Humans ; Male ; Middle Aged ; Spondylosis ; physiopathology ; therapy

Clustered regulatory interspaced short palindromic repeats (CRISPR) found in bacteria and archaea genome that contains multiple short repeats loci, provides acquired immunity against invading foreign DNA via RNA-guided DNA cleavage. The first inkling of this hot new genetic engineering tool turned up in 1987, when a research team observed an oddly repetitive sequence at one end of a bacterial gene. Now three types of CRISPR/Cas system have been identified: types I, II and III. In the type II CRISPR/Cas9 system, short segments of foreign DNA termed 'spacers' are integrated within the CRISPR genomic loci, transcribed and processed into short CRISPR RNA (crRNA). These crRNAs anneal to trans-activating crRNA (tracrRNA) and direct sequence-specific cleavage in that a double-strand break (DSB) is generated by Cas proteins. Based on these findings, various genetic methods, including gene targeting (Gene disruption), gene insertion, gene correction etc., are being designed to manipulate the genomes of different species at specific loci. Compared with zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), CRISPR/Cas9 is simpler with higher specificity and less toxicity. This review summarizes recent progress, discusses the prospects of CRISPR/Cas9 system, with an emphasis on its structure, principle, applications and potential challenges, and provides a useful reference for researchers who are interested in this new technique.
Bacteria ; CRISPR-Cas Systems ; DNA ; Genetic Engineering ; Genomics ; RNA

TheInner Canon of Huangdi originated from moral.In the aspect of knowledge, it advocatesthat the doctor should not teach bandits, selecting and teaching, upright character, open but not narrow;In terms of career pursuit, it advocatesthat the doctor should adopt and frequently ask, work hard and to be a good doctor;In the as-pect of ethics, it advocatesthatdoctor should cherish life, be meticulous;In terms of professional belief, it advocat-esthatdoctor should learn from the ancient, firmly believe that science, opposing superstition; TheInner Canon of Huangdi advocate consummate medical skillthe noble medical ethics, with far-reaching influence, become the ori-gin of Chinese medical ethics thought germinal, future generations of today′s high-quality professional talents of Chinese medicine has a very important guiding significance.

Objective:To explore the correlation between homocysteine (Hcy) level and left ventricular diastolic func‐tion in patients with hypertension .Methods :A total of 121 enrolled patients with essential hypertension received plasma Hcy ,routine biochemical measurement and echocardiography examination .With Hcy=10 mmol/L as the di‐viding line ,patients were divided into H hypertension group (high Hcy group ,n=65) and hypertension with normal level group (normal Hcy group ,n=56) .Correlation between plasma Hcy level and left ventricular diastolic function was analyzed .Results:(1) H hypertension accounted for 53.7% ,male proportion in H hypertension group was sig‐nificantly higher than that of normal Hcy group (67.7% vs .37.5% , P<0.01);(2) Compared with normal Hcy group ,there were significant rise in left atrial diameter [LAD ,(34.4 ± 3.3) mm vs .(37.6 ± 5.4) mm] ,left ven‐tricular mass [LVM ,(183.5 ± 37.0) g vs .(205.6 ± 53.1) g] ,left ventricular mass index [LVMI ,(111.1 ± 19.2) g/m2 vs .(121.2 ± 31.8) g/m2 ] and transmitral early diastolic peak flow velocity/peak velocity of early diastolic mi‐tral annular motion [E/Em ,(8.4 ± 2.6) vs .(15.3 ± 7.4)] in H hypertension group ,P<0.05 or <0.01 ;(3) Pear‐son correlation analysis indicated that Hcy was positively correlated with LAD ,left ventricular end‐diastolic dimen‐sion (LVEDd) ,E/Em ,LVM and LVMI (r=0.247~0.620 , P<0.05 or <0.01);multi‐factor linear gradual re‐gression analysis found that plasma Hcy level ,was influencing factors for E/Em (P=0.529 ,t=7.599 ,P<0.05) . Conclusion:There is a significant positive correlation between plasma homocysteine level and left ventricular diastolic function insufficiency in patients with hypertension .More attention is required for detection of plasma homocysteine level in clinic .

Objective To explore the characteristics of approach and avoidance behaviors among subthreshold depressed adolescents. Methods 27 subthreshold depressed adolescents and 29 healthy con?trol teenagers completed the approach?avoidance task,and the reaction time at approach and avoidance task of the two groups was compared. At the same time,their differences were analyzed between the two groups on the Behavioral Inhibition/Activation System Scale scores. Results ( 1 ) Subthreshold depression group (1208.18±338.92)ms) had significantly longer reaction time than those in healthy control group((1058.40 ±204.22)ms) when approaching happy expression ( t=2.02, P<0.05). (2) Subthreshold depression group (37.33±4.46) had significantly lower behavioral activation system scale scores than those in healthy control group ((39.76±3.75), t=-2.21, P<0.05); and the score of reward responsiveness was marginally signifi?cant lower in subthreshold depression group (12.63±1.67) than that in healthy control group ((13.41± 1.30), t=-1.97, P=0.054). Conclusion To activate the approach behaviors of subthreshold depression adolescents may be helpful for reducing the symptoms of depression.

Objective:To observe the therapeutic effect of Dahuang Jianzhong Decoction on functional dyspepsia(FD).Methods:120 cases of FD were divided into four syndrome types:deficiency-cold of the spleen and stomach,stagnation of the liver-qi,insufficiency of stomach-yin and accumulation of damp-heat,and they were treated for 4 weeks.The treatment group(n=80) were treated with Dahuang Jianzhong Decoction,and the control group(n=40) with Motilium.Results:The total effective rate for the clinical symptoms was 90% in the treatment group and 72.5% in the control group,with a significant difference between the two groups(P

This paper describes the after-sale service of medical equipment information management platform, with large data sharing resources to further enhance customer service in the whole management process of medical service, to strengthen quality management, to control medical risk.
Equipment and Supplies ; Information Management ; Product Surveillance, Postmarketing ; Quality Control

The plasma levels of atrial natriuretic factor (ANF), angiotension II (AII, vaso-pressin (VP) and endogenous digitalis-like factor (EDLF) were determined in 23 patients with mi-tral stenosis before and after percutaneous balloon mitral valvuloplasty (PBMV). Before PBMV, the plasma levels of ANF and EDLF were elevated in all patients with lower level of All than nor-mal. After the procedure, with a concomitant decrease of left atrial pressure, the plasma ANF de-creased at 15 minutes (from 317. 4?96. 2 to 164. 9?56. 8ng/L , P

0 05); At the 7th day, T?RⅠ mRNAs expression was decreased( P