Objective To observe the effect ofXuebijing injection on vascular endothelial function and inflammatory factor in the patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods A total of 100 patients with AECOPD were randomly divided into the study and control groups, 50 patients in each group. The control group were given the conventional therapy. The study group were given the Xuebijing injection on the basis of the control group. Both The changes of lung function, vascular endothelial function and inflammatory factor were observed.Results The expression of FVC (3.23 ± 0.83 Lvs. 2.79 ± 0.71 L,t=1.786), PEF (5.36 ± 0.51 L/svs. 4.79 ± 0.46 L/s,t=1.904), FEV1/FVC (59.21 ± 4.38vs. 54.94 ± 4.29,t=1.882), NO (3.84 ± 0.81 ng/mlvs.2.97 ± 0.62 ng/ml,t=1.869) increased significantly in the study group than those in the control group after treatenmt (P<0.05). The VEGF (0.14 ± 0.04 ng/ml vs.0.17 ± 0.05 ng/ml, t=2.097), TNF-a (0.11 ± 0.03 ng/ml vs.0.14 ± 0.02 ng/ml,t=1.903), IL-13 (107.27 ± 44.37 ng/mlvs. 136.54 ± 48.32 ng/ml,t=2.005) in the study group decreased significantly than those in the control group (P<0.05). ConclusionsXuebijing injection could significantly decrease inflammatory factor,and obviously improve vascular endothelial function and lung function,the clinical curative effect is distinct.

This article discussed the necessity and method of setting up a comparative imaging medicine course, which is considered to be valuable for the future development and creativity of graduate students majored in nuclear medicine and imaging medicine.

Objective To explore variance and resource of intracellular free Ca2+ and extracellular arachidonic acid (AA) in different types of passage cells during the invasion of T. gondii. Methods The variance and resource of extracellular AA and intraceUular free Caz+ of Vero and J774A. 1 cells during the invasion of T. gondii were detected by multi-purpose scintillation counter and laser scanning confocal microscope. Data were analyzed using Chi-square test and t test. Results The intracellular free Ca2+ levels in J774A. 1 and Vero cells were both increased after T. gondii infection. The maximal changes of fluorescence intensity were (1 219.7±58.4)% (P＜0.01) and (356.3±23.6)% (P＜0.05), respectively.The increase of intracellular Ca2+ level in Vero cell was mostly from the release of intracellular Ca2+ store.And the Ca2+ increase in J774A. 1 cell was from both the release of intracellular Ca2+ store and extracellular Ca2+ influx. Extracellular AA levels were significantly increased in both Vero and J774A. 1 cells after T.gondii infection 5.02 and 8. 44 times respectively (t= 3. 124, t = 3. 852, P＜0.01). The AA elevations could be significantly inhibited by phospholipase A2 (PLA2) inhibitor pretreating T. gondii. Conclusions The phospholipase C of phagocytic host cell and PLA2 of T. gondii are activated by T. gondii infection,which results in the increase of intracellular free Ca2+ and extraeellular AA level. Combined actions of Ca2+and AA play a major role in the invasion of T. gondii to host cell. While only PLA2 of T. gondii may be activated in nonphagoeytic host cell.

Objective To selectively resect the lymph nodes in the endometrial cancer by risk factors evaluation pre-and intra-operation.Methods Three hundred and seventy-four cases who in the Obstetric and Gynecology Hospital Affiliated to Fudan University from Oct.2010 and Dec.2012 were clinically diagnosed as stage Ⅰ pre-and intra-operation,grouped as one (staged Ⅰ A,294 cases) and two (staged Ⅰ B,80 cases),and the situation of lymph nodes metastasis and the related risk factors were analyzed.Results (1) Lymph nodes metastasis were positive in 6 cases in group one (2.0％) and 12 cases in group two(15.0％),and the difference was significant between two groups (x2 =23.054,P =0.000).(2) University analyses showed that pathologic type,grade,myometrial invasion,lymphovascular invasion and cervical gland invastion were the risk factors contributed to the lymphatic metastasis in early staged endometrial cancer (P＜ 0.05).Multivariate analysis showed that myometrial invasion was independent risk factor affecting early metastasis of lymph node in endometrial carcinoma (regression coefficient =2.339,standard error =0.585,OR =10.897,95％ CI 3.273 ～ 32.367,P =0.000).(3)Based on the gynecology oncology group test and risk factors that recent used in different countries and clinical centers,superficial myometrial layer invasion,G2,age＞ 60 years,tumor ≥ 2 cm in diameter as the medium risk factors,and deep myometrial layer invasion,G3,lower portion of the uterus or cervical gland invasion as high risk factors,and patients with two or more medium risk factor (106 cases) and with one or more high risk factors(138 cases) were inclined to develop lymph node metastasis((5.66％ (6/ 106),0(0/130),P =0.04;8.70％ (12/138),2.54％ (6/236),P =0.007).(4) Two hundred and thirteen patients were found with superficial layer invasion by frozen section,and 11 of them were upgraded to Ⅰ B by postoperative pathologic examination.Interestingly,all of the 11 cases were accompanied by two medium or one of high risk factor.Conclusion Low incidence of lymph nodes involvement is found in stage Ⅰ endometrial cancer,especial stage Ⅰ A.Myometrial invasion is closely linked to lymph node metastasis,thus,lymphadenectomy should routinely be carried out in endmetrial cancer diagnosed as Ⅰ B whether pre-or intraoperation.As in stage Ⅰ A,unless two medium and one high risk factors are found.

Objective To study a multiple frequency tympanometry on ear system. Methods Based on the original low frequency and single ingredient SY-IA tympanometry, the study mainly focused on such aspects as multiple frequency probe tone circuit, constant pressure sound control system, micropressor control system and multiple frequency acoustic immitance & phase angle detection circuit. Results Multiple frequency tympanometry on ear system could detect multiple frequency acoustic immitance and phase angle for normal ear, tympanum loosed ear and ossicular chain break ear under different probe tone. Conclusion Experiments show that multiple frequency tympanometry on ear system based on ARM is helpful to the diagnosis of pathological changes which are insensitive to single low frequency tympanometry.

Objective To keep the intensity of sound constant and reduce the effect of ear canal difference on detection process.Methods Real-time detection of the probe tone in ear canal was carried out and by control ling the output circuit of the probe tone through the digital signal compensation algorithm,to realize real-time digital tympanometry of ear system and the function of constant pressure control.Results The results showed that the probe tone remained constant at 226 Hz for the measurement of middle ear and tympanometry was accurate.Conclusion The study simplified system configuration and provides basis for the clinical application.

ObjectiveTo explore the relationship among resilience,attribution,and coping style for freshmen.Methods 977 freshmen were surveyed with Connor-Davidson Resilience Scale,Multidimensional-Multiattributional Causality Scale,and Simplified Coping Style Questionnaire,then compared different freshmen's resilience,attribution,and coping style,and analyzed their relationship.ResultsThe city freshmen showed significant higher resilience(68.88 ± 10.57 ),academic external attribution (21.14 ± 6.01 ),and interpersonal external attribution ( 22.87 ± 6.42 ) than rural freshmen ( ( 67.51 ± 10.43 ),( 19.81 ± 5.88 ),( 21.86 ± 5.88 ) respectively,t =2.03,3.48,2.57).Vocational freshmen showed significant higher academic internal attribution ( 28.68 ± 5.30 )and interpersonal internal attribution (26.81 ± 5.82) than undergraduate freshmen ( ( 27.61 ± 5.47 ),( 25.53 ±5.92),t =3.00,3.30).The only children showed significant higher resilience ( 68.88 ± 10.81 ) and academic failure luck (4.37 ± 2.21 ) than those having siblings ( (67.44 ± 10.18 ),(3.96 ± 2.12 ),t =2.15,3.02 ).Positive coping style,ncgative coping stylc,academic failure external attribution,academic success internal attribution,residence,and academic failure internal attribution had significant direct influence on resilience(β =16.435,- 4.919,- 0.344,0.308,1.396,- 0.182).ConclusionPositive coping styles and reasonable academic attribution can effectively predict great resilience of freshmen.

PurposeTo investigate the accuracy of18F-FDG PET/CT in diagnosing mediastinal and hilar lymph node metastasis of lung cancer and in guiding surgery. Materials and Methods Seventy-eight pathology-proven lung cancer patients underwent 18F-FDG PET/CT scanning and surgery. Histology was used as gold standard to evaluate the diagnosis of mediastinal and hilar lymph node metastasis.18F-FDG PET/CT was also compared to chest CT.Results The pathological examination confirmed metastasis lymph nodes in 105 out of 231 excised lymph nodes in 18 patients. No lymph node metastasis was found in mediastinum in the other 60 patients with lymph node staging of pN0. Lymph node staging was pN1 in 5 patients, pN2 in 11 patients, and pN3 in 2 patients. In all 78 cases, the sensitivity, specificity, accuracy, positive predictive value and negative predictive value of18F-FDG PET/CT were 80.9%, 94.7%, 91.0%, 85.0%, 93.1%; while 61.1%, 71.7%, 69.2%, 39.2%, 86.0% on chest CT. These were statistically different (χ2=4.325,P<0.05).Conclusion PET/CT is superior to plain chest CT examination as an accurate and efficient method in evaluating lymph nodes staging of lung cancer.

Objective To compare the efficacies of several commonly-used penetrating needling methods in treating post-stroke strephenopodia. Method Eighty-three patients with post-stroke strephenopodia were randomized into four groups, a control group intervened by conventional needling method (n=20), and the rest three groups were additionally intervened by different penetrating needling methods: penetrating group 1 [Qiuxu (GB40) towards Zhaohai (KI6), n=21], penetrating group 2 [Taibai (SP3) towards Shugu (BL65), n=21], and penetrating group 3 [Jiaoxin (KI8) towards Fuyang (BL59), n=21], to compare their effects on the function of ankle and lower extremity. Result The penetrating needling methods and conventional needling method all can significantly improve the function of ankle in patients with post-stroke strephenopodia (P<0.01). The effectiveness of penetrating group 1 was more significant than that of the rest groups (P<0.01), the effectiveness of penetrating group 2 was more significant than that of penetrating group 3 and the control group (P<0.05,P<0.01), and there was no significant difference in comparing the effectiveness between penetrating group 3 and the control group (P > 0.05). The penetrating needling methods and conventional needling method all can significantly improve the motor function of lower extremity in patients with post-stroke strephenopodia (P<0.01). The effectiveness of penetrating group 1 was more significant than that of the other groups (P<0.01), the efficacies of penetrating group 2 and 3 were more significant than that of the control (P<0.01), and there was no significant difference in comparing the effectiveness between penetrating group 2 and 3 (P>0.05). Conclusion Needling from Qiuxu towards Zhaohai and from Taibai towards Shugu can produce significant effects in improving ankle function and the motor function of lower extremity in patients with post-stroke strephenopodia, while needling from Jiaoxin to Fuyang doesn’t have notable advantage compared to the other penetrating needling methods and conventional needling method.

Objective To explore the change of cytoskeleton and the variance of Ca2+ in cultured cells during the invasion of Toxoplasma gondii. Methods Tachyzoites suspensions were gathered by routine method and used to infect phagocytic cells(J774A.1)and non-phagocytic cells (HUVEC). The ability of T. gondii invading into the cells and the influence of cytoskeleton inhibitor,colchicine and cytochalasin D,were observed by microscopy. The rearrangement of cytoskeleton of cells was observed by fluoromicroscopy. By using laser scanning confocal microscope,the variance of Ca2+ in J774A.1 and HUVEC was detected. Results Ca2+ increased greatly in J774A.1 during the invasion of T.gondii(P0.05). The microfilaments of J774A.1 were agglomerated during the invasion of T. gondii. Cytoskeleton inhibitor,cytochalasin D(P0.05)during the invasion and cytoskeleton was not changed. Cytochalasin D and colchicine showed little effect on the infection rate of HUVEC. Conclusion The concentration of Ca2+ increases greatly and cytoskeleton(mainly the microfilament)has been rearranged in phagocytic cell during the invasion of T. gondii,while both of them show no significant change in non-phagocytic cell.