Adult ; Decompression Sickness ; Diving ; Humans ; Male ; Middle Aged ; Occupational Diseases

Adult ; Hearing Loss, Noise-Induced ; etiology ; Hearing Tests ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Young Adult

Objective To investigate the relationship between anti-lymphocyte antibody (ALA) and clinical and immunological parameters in primary Sj(o)gren syndrome (pSS).The significance of ALA and lymphopenia in pSS was explored.Methods Serum samples were obtained from 143 patients with pSS.Clinical and laboratory data were collected.Sera from 45 healthy individuals were collected as the controls.Serum ALA IgG was detected by indirect immunofluorescence test (IIFT).x2 test and t test were used for statistical analysis.The reasons of lymphopenia in pSS patients were analyzed.The correlation between ALA,lymphopenia and clinical manifestations as well as laboratory parameters was analyzed.Results ① Of the 143 pSS patients,25.2％(36/143) were serological positive for ALA,which was significantly higher than the normal group (6.5％,5/77) (x2=27.496,P＜O.01).② The prevalence of leukopenia (55.6％,20/36),lymphopenia (77.8％,28/36),anti-nuclear antibody(80.5％,29/36) and C3 decrease(50.0％,18/36) was higher in pSS patients with ALA than those without (x2=8.296,25.348,3.929,3.866,P＜0.05).③ 46.2％(66/143) patients had lymphopenia.Comparing with patients with normal lymphocyte count,the incidence of ALA positive,leukopenia,PLT decrease and anti-SSB antibodies positivity was significantly higher in patients with lymphopenia (x2=27.496,51.344,5.320,8.950,7.782; P＜0.05).Conclusion The ALA is likely to be a meaningful sera marker for pSS patients with lymphopenia,and this suggests that it has an important role in the clinical progress of SS.

Objective To investigate the impact of bone marrow mesenchymal stem cell transplantation on a rat model of experimental autoimmune uveitis (EAU) and analyze its immune regulatory mechanisms in vivo. Methods Eighteen Lewis rats were randomly divided into three groups: model control group, intervention group and normal control group, six animals in each group. Human retinal S-antigen peptide (HS-AgP35, 1 mg/ml) was mixed and emulsified with complete Freund's adjuvant and injected into hind foot pad of rats on the first and eighth day to establish the animal model of EAU. For bone marrow mesenchymal stem cell transplantation, 1 ml of cell suspension (2 × 106 cells/ml) was injected into tail vein of the intervention group rats on the first day when the emulsified S-antigen was injected. EAU manifestation, pathological change and IFN-γ level were evaluated and compared among those three groups after two weeks. Results No abnormal signs were found in the eyes of rats in normal control group. The manifestation grading of the intervention group (two rats at grade 0, three rats at grade 0.5, one rat at grade one) was significantly different from the model control group (one rat at grade one, one rat at grade two, three rats at grade three, one rat at grade four) (P=0. 015). The retina of rats in normal control group was ordinary under light microscope. The histopathological grading of the intervention group (one rat at grade 0, four rats at grade 0.5, one rat at grade one) and the model control group (four rats at grade three, two rats at grade four) was also statistically different (P＜0. 01). Furthermore, the IFN-γ level in peripheral blood of the intervention group rats declined significantly compared to the model control group (t=9. 057 4, P=0. 01). Conclusions Bone marrow mesenchymal stem cells can inhibit EAU significantly,possibly by lowering the level of IFN-γ, thereby reduce the severity of uveitis and improve the condition of uveitis in rats.

Objective To evaluate the quality of autoantibodies detection in China. Method Standardized samples were sent to 73 laboratories that are able to detect autoantibodies. The autoantibodies detected included antinuclear antibody (ANA), anti-dsDNA antibody, anti-extractable nuclear antigen (ENA) antibody, anti-mitochondrial antibody (AMA) and anti-smooth muscle antibody (ASMA). The analysis of testing results was double-blinded. Result The consistent rates of ANA, anti-dsDNA antibody, anti-ENA antibody, anti-mitochondrial antibody and anti-smooth muscle antibody were 86.2%, 75.4%, 62.7%, 100% and 61.1% respectively. Conclusion The overall accuracy has been elevated, but still needs to be improved.

OBJECTIVE To investigate the influence of noise on the hearing of the workers with noise exposureand explore the relationship between the hearing damage and the length of noise exposure.METHODS 120 workers who exposed to noise were recruited. Their physical examination including audiometry was done in our hospital from year 2012 to 2014. Hearing loss and the length of noise exposure were detected in all subjects after 2-year's follow-up.The rate of hearing loss between workers with different length of noise exposure was analyzed statistically. RESULTS There were 22 workers who were exposed to noise for 2 years, 43 workers for 3-5 years and 55 workers for 6-10 years. After 2-year's follow up, there were significant differences between the rate of hearing loss and the length of noise exposure at 3,4 and 6 kHz. And the rate of hearing loss at different sound frequency were significantly different statistically. CONCLUSION The relevance ratio of hearing loss in long noise-exposed group was significantly higher than short groups. The hearing damages at 4 KHz were more frequent than other sound frequency.

Objective To explore the role of apelin in the pathogenesis of diabetic nephropathy.Methods A total of one hundred and fourteen patients with type 2 diabetes mellitus in Qingdao Municipal Hospital from July 2015 to June 2016 were divided into normal albuminuria group (56 cases in the NA group) and micro-albuminuria group (58 cases in the MA group),depending on whether the level of urinary micro-albumin in 24 h was higher than 30 mg,with 60 healthy subjects included as the controls group (NC group).Levels of serum apelin and other biochemical indexes were tested.Results (1) WC,BMI,TC,TG,LDL-C,FBG,FINS,HOMA-IR and apelin in the patients of the NA group and MA group were respectively (87.99±10.27) cm,(24.75±3.35) kg/m2,(5.27±1.28) mmol/L,(1.84±1.25) mmol/L,(3.36±0.91) mmol/L,(7.58±0.87) mmol/L,(13.29±3.57) U/L,4.50±1.41,(364.22±85.03) ng/L ,(90.10±8.97) cm,(25.47±2.82) kg/m2,(5.19±1.11) mmol/L,(2.23±1.43) mmol/L,(3.68±0.83) mmol/L,(7.89±1.11) mmol/L,(15.50±5.00) U/L,5.55±2.27,(397.42±91.29) ng/L,which were higher than those of the NC group ((83.20±5.36) cm,(22.59±2.67) kg/m2,(4.68±1.10) mmol/L,(1.37±0.58) mmol/L,(2.56±0.94) mmol/L,(5.11±0.82) mmol/L,(7.17±2.80) U/L,1.65±0.77,(309.34±68.28) ng/L,P<0.05).FINS,HOMA-IR and apelin in the MA group were significantly higher than those in the NA group (P<0.05).(2) After age,sex and BMI were taken into control,partial correlate analysis showed that serum apelin was positively correlated with LDL-C,FPG,FINS and HOMA-IR (r=0.183,0.314,0.374,0.378,P<0.05).(3) Multifactor regression analysis showed that HOMA-IR and BMI were independent related factors to serum apelin (r2=0.288,0.389,P<0.05).Conclusion Apelin levels in the MA group were significantly higher than those in the NA group,and the serum apelin was positively correlated with HOMA-IR.Thus,apelin may contribute to the pathogenesis and progress of diabetic nephropathy.

Objective To investigate the state of oxidative stress in the subjects with different levels of serum uric acid and to explore the cause of endothelial dysfunction induced by hyperuricemia. Methods Male subjects with normal serum uric acid or hyperuricemia were enrolled in this study. According to the levels of serum uric acid,all the partieipante were divided into five groups. Every group consisted of about fifty subjects. Plasma malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px) were determined. In the mean time plasma nitric oxide(NO) ,plasminogen activator inhihitor-1(PAI-1), endothelin-1 (ET-1) and other biochemical variables were also determined. Results When the serum uric acid level was more than 380 μmol/L, the levels of plasma MDA,SOD,GSH-Px, PAI-1 and ET-1 became higher, while the level NO became lower(P<0.05). However SOD and GSH-Px became much lower when the serum uric acid level was more than 420 μmol/L. Multivariate stepwise regressive analysis showed that PAI-1 was positively related to MDA,UA,HOMA-IR and TG, but negatively related to SOD and NO(t =-3.64 - 6. 08,P < 0.05). ET-1 was positively related to MDA, UA, HOMA-IR and negatively related to NO, GSH-Px and SOD (t = - 4.75 - 6.35,P < 0.05 ). Conclusions It is indicated that oxidative stress became much obvious when the serum uric acid level was more than 380 μmol/L . Oxidative stress,high serum uric acid level and insulin resistance may result in endothelial dysfunction.