Objective To compare the content of polysaccharide in Herba Scutellariae Barbatae derived from different places. Method Anthrone-sulphuric acid colrimetry was used. Result The contents of polysaccharide in Herba Scutellariae Barbatae derived from Mojiang Yunnan, Nantong Jiangsu, Huaning Hunan, Yongding Fujian, Lixin Anhui, Jiaoling Guangdong, Nanchang Jiangxi, Longsheng Guangxi, Honghu Hubei and Qingyuan Zhejiang were 8.12%, 7.61%, 6.61%, 6.07%, 5.84%, 4.61%, 3.15%, 2.69%, 2.65% and 2.34%, respectively. Conclusion The content of polysaccharide in Herba Scutellariae Barbatae from different places is quite different, and the highest is the one from Mojiang Yunnan.

Objective:To investigate the mechanism of ursolic acid inducing the apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z.Methods:CNE-2Z were treated by ursolic acid with different concentration.Cell proliferation was determined by MTT assay.Cell cycle and apoptosis rate were analysed by flow cytometry(FCM).The expressions of bax,bcl-2 and cox-2 were assessed by SP method of immunocytochemistry,while the expression of caspase 3 by Western blot.Results:Ursolic acid inhibited the proliferation of CNE-2Z cells and induced the apoptosis in dosage and time dependent manner.FCM showed that the cell population reduced in S phase and cell cycle was arrested in G0/G1 phase.Immunocytochemistry showed that the expression of bax was up-regulated in the apoptosis,while the expression of bcl-2 and cox-2 down-regulated.Western blot showed that the expression of caspase 3 was strengthened.Conclusion:Ursolic acid can induce apoptosis of CNE-2Z in vitro.Its molecular mechanism probably is through promoting activation of caspase3,up-regulating the expression of bax,and down-regulating the expressions of bcl-2 and cox-2.

Objective To compare the content of saponin in Bark of Silktree Albizziae and Bark of Silktree Albizziae kaldora derived from different collection periods.Method Spectrophotometry was used to determine the contents of saponin.Result The contents of saponin in one-year Bark of Silktree Albizziae is different from two-year Bark of Silktree Albizziae(P0.05).The contents of saponin in multi-year Bark of Silktree Albizziae is different from multi-year Bark of Silktree Albizziae kaldora(P

Objective:To identify differential proteins expression of seminal plasma of varicocele patients and normal person,providing evidence for further exploring the molecular mechanism of infertility caused by varicocele.Methods:The differential proteins expression of seminal plasma of varicocele patients and normal person were characterized by means of two-dimensional gel electrophoresis(2-DE).Results:The well-resolved,reproducible 2-DE seminal plasma were obtained.The 38 differential protein spots were identified.Conclusion:The male infertility might due to the expression and modification of some proteins in seminal plasma of varicocele patient.Further analysis on the different proteins'expression helps revealing molecule mechanism of varicocele causing male infertility.

Objective:To study the influence of rt-PA and urokinase on the expression of matrix metalloproteinase(MMP-9) and hemorrhagic transformation after thrombolysis therapy in rats.Methods:A focal cerebral ischemia model of rat was established through embolization of left middle cerebral artery with autologous blood clots.rt-PA and urokinase were intravenously administrated 6 hours after ischemia at a dose of 10 mg/kg and 4 000 ?/kg.At the 24th hour after ischemia,all rats were killed.The expression of MMP-9 was detected with immunohistochemical staining and cerebral hemorrhage was quantified by spectrophotometer assay.Results:The expression of MMP-9 was increased after ischemia,and MMP-9 was significantly further upregulated after rt-PA and urokinase had been intravenously administrated.The average optical density(AOD) of MMP-9 in the sham operated group was 0.086?0.008,and increased to 0.158?0.010 after cerebral ischemia for 24 hours and then further increased to 0.225?0.034 and 0.228?0.022 in the rt-PA and urokinase thrombolytic therapy groups.Transformation and quantified intracerebral hemorrhage were significantly higher in the therapy groups as compared with the ischemic group.However,there was no difference between rt-PA group and urokinase group.Conclusion:rt-PA and urokinase treatment both increase the levels of MMP-9 after ischemia.MMP-9 might be involved in the hemorrhagic transformation after thrombolytic therapy with rt-PA and urokinase.

Objective To compare the content of total flavonoids in Herba Scutellarlae Barbatae derived from different places.Method UV metric method was used to determine the contents of total flavonoids of Herba Scutellarlae Barbatae from different regions.Result The contents of total flavonoids in Herba Scutellariae Barbatae derived from Nantong Jiangsu,Juaoling Guangdong,Lixin Anhui,Huaning Hunan, Mojiang Yunnan,Yongding Fujian,Nanchang Jiangxi,Longsheng Guangxi,Honghu Hubei and Qingyuan Zhejiang were 4.16%,3.55%,2.89%,2.78%,2.41%,2.22%,2.16%,1.98%,1.61% and 1.51%,respectively. Conclusion The content of total flavonoids in Herba Scutellariae Barbatae from different places is quite different,and the highest is the one from Nantong Jiangsu.

Objective To discuss the change and the regulation of tumornecrosis factor(TNF-?) and epidermal growth factor(EGF) before and after extracorporeal shock wave lithotripsy.Method Radioimmunoassay was used to measure TNF-? in 50 cases urine sample before and after ESWL.Results TNF-? level in urine after ESWL were increased significantly at 1～4d(t=2.82,P

OBJECTIVE: To study of effects of dexarmethasome of Jianlishu Powder on the release of tumor necrosis factor-? in rats of adjuvant arthritis model. METHODS: Adjuvant-induced arthritis (AIA) was induced by injection of frenndi's complete adjuvant in rats' plantar to observe the effects of Jianlishu Powder on rice' hypersensitivity reaction, thickness of inflamed plantar of both feet, and levels of tumor necrosis factor-?(TNF-?), Interleukin-2 (IL-2) and circulating immune complex (CIC) in AIA rats. RESULTS:rats' right posterior metatarsus which was injected with adjuvant was swollen from the second day for 15 days (primary lesion). Jianlishu Powder significantly inhibited feet swelling induced by collagen dose-dependently and down-regulate the contents of TNF-?,IL-2 and CIC thus resulting in an anti-inflammatory action in AIA rats(P

OBJECTIVE: To discuss the transnational management strategy of traditional Chinese medicine(TCM) enterprises in China.METHODS: The actuality of the transnational management of TCM enterprises and the main difficulties encountered by them were analyzed.RESULTS: The problems such as foreigners' different opinions on TCM,the laws,intellectual property rights and technology faced by TCM enterprises hindered the practice of the strategy of transnational management.CONCLUSION: The TCM enterprises in China should rely on special advantages in TCM to cultivate the ownership advantages,regional advantage and internalization advantage so as to effectuate transnational management.

OBJECTIVE: To promote Chinese pharmaceutical enterprises to understand their social responsibility clearly and to fulfill it actively.METHODS: The category of social responsibility of pharmaceutical enterprises was analyzed as well as the main approaches to perform corporate social responsibility.RESULTS: Chinese pharmaceutical enterprises should figure out their responsibility to consumers,employees,investors,society,community and other stakeholders.In recent years,the approaches to perform social responsibility based on force system of law and regulations and the improvement of enterprises' consciousness.These are unfavorable to Chinese pharmaceutical enterprises to undertake social responsibility actively.CONCLUSION: There are two approaches to promote Chinese pharmaceutical enterprises to perform their social responsibility,which are external supervision and internal governance.