Objective To investigate the levels of homocysteinin and neutrophil gelatinase associated lipocalin and its rela-tionship in patients with chronic heart failure combined insufficient renal function .Methods 58 patients with chronic heart failure were enrolled in from June 2013 to June 2014 in Beijiao hospital affiliated Southern medical university .Thereinto, according to pa-tient’s condition, 28 patients were chronic heart failure concurrence insufficient renal function, 30 patients were chronic heart failure. Left ventricular ejection fraction were determined by cardiac ultrasound , the levels of serum BNP, Hcy, NGAL and creatine were de-termined.Results Compared with chronic heart failure patients,the levels of Hcy and NGAL were increased in patients with chronic heart failure concurrence insufficient renal function (p<0.05).Correlation analysis showed that the levels of Hcy is positively corre-lated with the levels of NGAL (p<0.05);the levels of Hcy and NGAL were positively correlated with BNP and creatine (p<0.05), but negatively correlated with LVEF (p<0.05), respectively.Conclusion Circulating levels of Hcy and NGAL can reflect the de-velopment of chronic heart failure concurrence insufficient renal function .

Objective To investigate correlational study on the levels of homocysteinin with the severity of coronary artery in patients with coronary atherosclerotic disease.Methods 60 patients with coronary atherosclerotic disease were enrolled in from Janu-ary 2013 to December 2014 in Beijiao hospital affiliated Southern medical university .All patients received coronary artery angiograph and the levels of circulating homocysteinin were determined.According to the levels of circulating homocysteinin, 60 patients were de-vided into coronary atherosclerotic disease group and coronary atherosclerotic disease group with hyperhomocysteinemia ( HHcy) .The levels of homocysteinin and the severity of coronary artery in patients with coronary atherosclerotic disease and correlation between the two group were analyzed.Results Compared with coronary atherosclerotic disease patients,the levels of homocysteinemia and Genisi score were increased in patients with coronary atherosclerotic disease with HHcy (p<0.05).Correlation analysis showed that the lev-els of homocystein is positively correlated with Genisi score (p<0.05).Conclusion HHcy is a important risk for coronary athero-sclerotic disease and positively related with the severity of coronary artery in patients with coronary atherosclerotic disease .

A novel strategy employing dendrimer to multi-label the template molecule to improve the sensitivity of molecularly imprinted electrochemical sensor was proposed. The determination relies on a competition reaction between poly ( diethylene-triaminepenta-acetic acid )-glycol ester ( PDTPA )-ferrocene-carboxylic acid ( FcA ) labeled gibberellins acid 3 ( GA3 ) and GA3 in the sample. Since one cavity corresponds with multiple FcA, instead of only one FcA, the intensity of the detecting signal was greatly enhanced, so was the sensitivity of the sensor. Experimental results showed that the molecularly imprinted electrochemical sensor was sensitive to GA3 detection at a concentration from 2. 0í10-9 to 1. 0í10-7 mol/L, with a detection limit of 9. 3í10-10 mol/L. In addition, the sensor had good reproducibility and its feasibility was verified in the analysis of series of real beer samples.

Objective To explore the value of T wave alternans (TWA) in prediction ventricular arrhythmias in post-op TOF children. Methods The characteristics of TWA were retrospectively analyzed in 36 post-op TOF patients and 120 age-matched normal children. The relationship of sustained TWA and ventricular arrhythmias was also analysed. Results Compared with normal children, the incidence of sustained TWA was significantly increased in post-op TOF group (χ2=6.98, P<0.05). In post-op TOF patients, the incidence of ventricular arrhythmia was much higher when sustained TWA was present (χ2=10.41, P<0.05). Conclusions There exists electrophysiological basis for ventricular arrhythmia in post-op TOF children. Sustained TWA has important value in prediction of ventricular arrhythmia of post-op TOF.

Objective To investigate the mean values of carotid intima-media thickness (IMT) and change features according to gender,segments and age groups in general Chinese population,and provide the basis for the health assessment of carotid ultrasound.Methods GE LOGIQ 7 ultrasound scanner was used to measure IMT of 743 healthy cases aged from 38 to 70 years old.Six segments(bilateral common carotid artery,bilateral carotid bulb,bilateral internal carotid artery) were measured for each case.All patients were divided into 6 age groups across 5 years to be one group.The mean values for each group was calculated.Results The average age of the three sections of carotid IMT gradually increasing linearly related; minimal change in men and women,the magnitude of the age of the internal carotid artery IMT;sinus IMT were greater than the common carotid artery IMT and carotid artery IMT thinnest.the mean carotid IMT of Men and women in the same age group,compared to the 46-50 group,the group of 51-55,56-60 group,the differences were significant (P ＜ 0.05);men and women 41-45 group and 61-65 difference between the groups was not statistically significant (P ＞ 0.05);mean IMT difference of the male groups compared with the older group of women was not statistically significant (P ＞ 0.05).Conclusion Ultrasound scanner can be used to measure IMT accurately.In the 46-60 group,carotid IMT thickening in men than women about 5 years earlier.The mean IMTs for different sex,age groups and segments would greatly benefit vascular health evaluation.It has a great significance in the prevention of cardiovascular and cerebrovascular diseases.

Application of Radio Frequency Identification (RFID) into transfusion management in hospital wards,for elimination of errors from transfusion.Its clinical practice in three years has significantly improved transfusion efficiency,optimized workflow and reduced nursing errors.In the meantime,it has improved informationization of supplies satisfactorily.The paper also discussed the merits and defects of RFID for reference and review of the readers.

BACKGROUND:In recent years, with the development of China Marrow Donor Program and the improvement of human leukocyte antigen (HLA) typing technique, novel aleles of human leukocyte antigen have been discovered constantly in China. OBJECTIVE:To identify and confirm a novel HLA alele in a Chinese individual. METHODS: A new HLA alele was found during routine human leukocyte antigen genotyping by PCR-sequence specific oligonucleotide probes and sequencing-based typing. RESULTS AND CONCLUSION:The HLA-B locus hybridization probe reaction patterns of this sample did not match with any known HLA-B aleles or alelic combinations. Exons 2, 3 were sequenced in both directions using HLA-B sequence primer and group-specific sequencing primer. The obtained sequence had 2nts change from B*07:02:01 at nt 226 and nt 228 where A->G (codon 76 ATA->GTG) and resulting in a coding change, 76 isoleucine (I) was changed to valine (V). This nucleotide sequence has been submitted to the GenBank nucleotide sequence database and it is available under the accession number HM989017. A novel HLA alele, HLA-B*07:110, was identified, and was named officialy by the WHO Nomenclature Committee.

Objective:To explore the counselor verbal response modes of psychic hot-line counseling and its characteristics.Methods:Through a qualitative study with category analysis method,8 pieces of audio recordings were collected,in each of which the entire duration was between 30 minutes to 50 minutes.And all of these record-ings were transcribed word by word.Meaningful 1202 pieces were deeply analyzed.Results:There were 5 core counselor verbal response types in hot-line counseling,greeting response,set-up response,listening response,follow-up response,and affecting response,all of which had 24 concept categories.All of the online counselors used the 5 types of verbal response.The listening response and follow-up response were the highest frequent types.Greeting response and set-up response were used mostly in the initial stage of counseling.Listening response,and follow-up response were used mostly in the stage of describing problem.Affecting response was sharply increased in the stage of analyzing problem.Greeting response and set-up response were used mostly in last stage of counseling.Conclu-sion:It suggests that there are 5 core counselor verbal response types and 24 concept categories in hot-line counse-ling.The listening response and follow-up response are the highest frequent types.Different types of verbal re-sponse modes used by counselor in different stages due to different intention.

BACKGROUND:In recent years, the use of chimeric antigen receptor modified T cells (CAR-T cells) has achieved good results in the treatment of hematological malignancies. OBJECTIVE:To summarize the CAR-T technology, and its anti-tumor mechamism, progress in the treatment of hematological malignancies as well as its safety and coping strategies. METHODS:A search of PubMed, CNKI, Wanfang by computer was performed for articles related to CAR-T published from January 2010 to January 2016, using the keywords of CAR-T in English and Chinese, respectively. RESULTS AND CONCLUSION:The main principles that CAR-T cells fight against tumors are as follows: (1) resistance to immune escape through down-regulation of MHC; (2) production of interleukin-6, -10 indirectly influences the growth of tumor cells; and (3) tumor microenvironment changes inhibit tumor growth. The use of CAR-T in the treatment of hematological malignancies has been developed by leaps and bounds in recent years. Current studies concerning B cell lymphoma mainly focus on anti-CD19 CAR-T cells that have certain therapeutic effects on acute myeloid leukemia and acute lymphoblastic leukemia. However, its safety and effectiveness have yet to be studied.

The development of liver surgery can be divided into several stages, including wedge liver resection, regular segmental liver resection and anatomical liver resection. With the development of biomedical sciences and information technology, liver surgery has developed into precise hepatectomy. The goals of precise hepatectomy are minimal invasion, maximal liver protection and best rehabilitation, This is mainly achieved through accurate preoperative evaluation, precise surgical planning, sophisticated surgical procedure and postoperative management. Its application significantly improves prognosis and quality of life of patients. This paper summarizes the concept and surgical technique of precise liver resection.