Objective To study the information absorbency of Chinese Journal of Radiation Oncology and it's half-citing life, find out the core journals and characteristics of literature requested by scientists, and analyze the trend of Radiation Oncology research. Methods The citation analysis is used to analyze the references cited in 475 articles in Chinese Journal of Radiation Oncology from 2001-2004. Results Citation rate is 70.5%,and there are 8.6 citations per article. Main types of citations are journals(91.7%) and books(7.8%). The Price Index is 41.6%,and the self-citation rate is 6.2%. The percent of citations from the high-ranked 20 journals are 57.0%. Conclusions The articles in Chinese Journal of Radiation Oncology concern most journals in the medicine,the most citations are from the English journals. The half-citing life becomes longer than research in 2003. It shows that the use of newly-published articles should be improved.

Objective To obtain the overall picture of the PubMed cited publications by major domestic medical institutions in 2013;and to learn the distribution of the number of publication,published journals,impact factor of journal,and articles published in China and overseas.Methods Information was retrieved from PubMedplus database,and the statistical analysis was conducted to assess the distribution in the number of publications,published journals and impact factor of journals by major medical institutions in China.Results Top 30 medical institutions of China in 2013 published 12178 PubMed cited papers on 1890 journals,in which 10602 papers were in English accounted for 87.06％,and 1575 papers were in Chinese accounted for 12.93％.2552 papers were Published in the top 13 journals accounted for 20.96％.PLoS One published the most of total papers.Conclusions The level of journal that published papers for major domestic medical institutions needs to be improved,and each medical institution has its own advantage in different disciplines.

Objective To understand clinical specimen hemolysis Staphylococcus (SHA)distribution characteristics and re-sistance and sensitivity to 20 kinds of antibiotics,reasonable to guide the clinical treatment of SHA infection.Methods Rou-tinely cultured and isolated bacteria.Used the United States BD Phoenix-100 automated microbial identification and suscepti-bility instrument to identify bacteria and susceptibility testing,and susceptibility testing all used the instrument broth dilu-tion method,according to the USA CLSI2015 [1]regulations standards.Results 162 strains of SHA from the distribution of age,children under the age was one of the highest (30.9%),and from the distribution department,mainly distributed in ped-iatrics (30.9%),department of critical care medicine (22.2%),medicine (17.3%),surgery (12.3%).From the specimen type distribution,were mainly distributed in the blood (33.3%),sputum (25.9%),wound (11.1%) and discharge (9.9%).In the 162 strains of SHA,the proportion of the MRSH was 93.8%,of which 152 strains of MRSH incidence of multiple drug resistance (MDR)was as high as 61.8%.Compared with MSSH,antibiotic resistance rate of MRSH was sig-nificantly higher.The resistance rate of MRSH to ampicillin,cefoxitin,penicillin G,erythromycin was extremely high,more than 98.7% the former of cefoxitin,penicillin G,ampicillin,erythromycin resistance was extremely high,more than 98.7%. The sensitive rate of both to rina thiazole amine,vancomycin,amikacin was 100%,and the rate to Fusidic acid,teicoplanin, nitrofurantoin was also high,more than 9 5.5%.Conclusion Linezolid,Vancomycin,Amikacin,Fusidic acid,Teicoplanin and Nitrofurantoin because all can be used as empiricaluse of SHA infection,other antibiotics chooses to in addition to drug sen-sitivity tests results.

Objective To study the effect of simvastatin on high glucose induced vascular endothelial cells injury and explore its action mechanism .Methods Endothelial cells were devided into four group:control group(A) ,high glucose(33 .3 mmol/L)group (B) ,high glucose(33 .3 mmol/L)+simvastatin(1 .0 μmol/L)group(C) ,and high glucose(33 .3 mmol/L)+simvastatin(10 .0 μmol/L)group(D) .The inhibition of cells was detected by CCK-8 assay .The apoptosis was determined by TUNEL assay .The protein ex-pression was detected by Western blot analysis .Results The cells survival rates were (42 .5 ± 6 .4% ) ,(58 .6 ± 7 .8% ) ,and (71 .3 ± 11 .7% ) in B ,C ,and D group respectively .There was significant difference among the three groups(P<0 .05) .The per-cents of apoptosis were (1 .8 ± 0 .6% ) ,(45 .8 ± 8 .9% ) ,(22 .7 ± 6 .4% ) and (12 .6 ± 4 .2% ) in A ,B ,C and D group ,respectively . There was significant difference among the four groups(P<0 .05) .The protein expression of Bcl-2 was lower in B group(0 .13 ± 0 . 03)than that in A group(1 .02 ± 0 .16) .The Bcl-2 expression was higher in C group(0 .28 ± 0 .04)than that in B group ,and in D group(0 .68 ± 0 .11)than that in C group(P<0 .05 ,P<0 .01) .The gene expression of bax was higher in B group than that in A group .The bax expression was lower in C group than that in B group ,and the bax expression was lower in D group than that in C groups(P<0 .05 ,P<0 .01) .Conclusion Simvastatin can inhibit high glucose induced vascular endothelial cells apoptosis .Upregu-lation of Bcl-2 and downregulation of bax may be its action mechanism .

AIM: To evaluate the clinical effects of compound trabeculectomy for primary angle-closure glaucoma with persistent high intraocular pressure.
●METHODS:Thirty-three cases ( 34 eyes ) with primary angle - closure glaucoma were executed compound trabeculectomy. All patients were divided into two groups: group A: 18 cases ( 18 eyes ) were executed compound trabeculectomy under high intraocular pressure higher than 35mmHg after using lOP-lowering medicine for 48-72h; group B: 15 cases (16 eyes) were executed compound trabeculectomy, the intraocular pressure was normal after using lOP-lowering medicine.
●RESULTS:Thirty-four eyes were successfully completed surgery with follow-up of 6 to 18mo and there were no serious complications, including suprachoroidal hemorrhage. The visual acuity before operation in group A and group B were 0. 02 ± 0. 01, 0. 04 ± 0. 02, respectively. And the postoperative visual acuity in group A and group B increased 0.2±0. 06, 0. 3±0. 07, respectively (P<0. 01). All of them, intraocular pressure with 30 eyes were controlled in 9-23 mmHg, 3 eye was controlled by normal through adding in several kinds different lOP-lowering medicine. One eye was failure. Compared to preoperation, postoperative intraocular pressure of two groups were decreased by normal, which were 17. 9 ± 9. 1 mmHg and 15. 4±8. 4mmHg, respectively (P<0. 01). There was no significant difference between two groups for postoperative intraocular pressure.
●CONCLUSION: The primary angle-closure glaucoma with persistent high intraocular pressure should be executed compound trabeculectomy decisively, even under high intraocular pressure, which prevent further damage and loss of visual function. As long as to fully consideration the preoperation, intraoperative careful operation, careful nursing, it is safety and effective to have compound trabeculectomy under the continuous high intraocular pressure for primary angle-closure glaucoma.

Objective To evaluate toxicopathological changes of central nervous system in rats at different morphine-dependent time. Methods Animal model of morphine-dependence in rats was established by subcutaneous injection of morphine. Histopathologic changes of coeruleus, periaqueductal gray, substantia nigra, lenticula, corpus amygdaloideun and hippocampus in morphine-dependent rats were observed by microscopy and electron microscopy, and synaptic numbers were counted and compared with those in control animals. Results Six morphine dependence-related regions in morphine-dependent groups were observed. Pyknosis or swelling of the nerve cells, swollen nerve fibers, swollen and deformed mitochondria were detected. Dilated endoplasmic reticulum and disaggregated polyribosomes were observed with increased number in synapsis. Gliocytosis and subpial infiltration of lymphocytes and monocytes became more obvious with extension of dependent time, and glial nodules were formed. Synaptic numbers were significantly increased in morphine-dependent groups at 4 or 8 weeks as compared with those in control group(P

Objective To isolate and classigy the bacteriophages specific to Pseudomonas aetuginosa and to investigate biofilm control efficaey of the isolated virulent phages.Methods With P. aeruginosa clinical strains as indicators.bacteriophages were isolated by screening difierent environmental samples.Classification of the isolated phages was done with the methods of restriction fragment analysis of phage genome and host range analysis.Transmission electron microscopy(TEM)was used in phage morphology study.In biogilm control tests,TJC729 was used as the jndicator strain to study the biofilm control efficacy of the isolated phages.Results Total 13 lytic phages specific to P.aeruginosa strains were isolated and named as C1-C13.According to the result of restriction fragment analysis.all 13 phages were double-stranded DNA viruses and could be divided into eight groups.Host range experiments were conducted with 5 laboratory strains and 12 clinical strains of P. aeruginosa.The same infection profiles were observed among phage C1 and C13,C6 and C7,and C9 and C11,respectively.While the remaining 7 phages each had different unique infection profile.Phage C1 was selected randomly to study its morphology.The obtained images showed that phage C1 had an icosahedral head with a non-contractile tail,belonging to the Siphoviridae family.Compared with the single phage,phage cocktail had the best effect on biofilm control.Further experiment results showed that phage C1.C10 and C12 can destroy biofilm after treatment of the biofilm for 24 h.The biofilm amounts were deceased to 32.7%,57.6%and 32.8%of the initial values,respectively.Conclusion Thirteen virulent phages specific to P. aeruginosa had been isolated.The phages could significantly inhibit the biofilm formation and had a certain degree of damage on the biofilm.The results suggested an alternative method for the treatments of P.aeruginosa infections.

Qualitative and quantitative analyses of biological samples containing drugs, toxicants and endogenous substances play an important role in the researches of life sciences, as well as in new drug discovery and development. Biological samples are characterized by complex matrix, multiple endogenous interferences, significantly lower concentrations of measured analytes compared to endogenous components and small sampling volume. Consequently, it often requires bioanalysis methods with superior specificity, high sensitivity and good reproducibility. The two-dimensional liquid chromatography (2D-LC) technique, which allows for high peak capacity, significant reduced matrix effect and carryover of complex matrix samples and automated sample pre-treatment and analysis, has been the powerful solution to the separation and analysis of biological sample and widely applied to environment, food and pharmaceutical analysis. On the basis of introduction of the principle and equipments of 2D-LC, the application of this technique in the pharmacokinetics, toxicological and biological study was reviewed.

BACKGROUND:Porous tantalum, characterized as high porosity, low elastic modulus, high friction coefficient, stable biological properties, good compatibility and typical structural properties, has been the focus in medicine, especial y in orthopedics. ＜br> OBJECTIVE:To review the biological characteristics of porous tantalum, including its mechanical properties, compatibility, and biological activity. ＜br> METHODS:A computer-based search of PubMed, CSTJ, Wanfang and VIP databases was done for articles relevant to porous tantalum published from 1990 to 2014 using the keywords of“porous tantalum, biological character, orthopedic applications”both in English and Chinese. ＜br> RESULTS AND CONCLUSION:Porous tantalum, with high porosity, low elastic modulus, high friction coefficient, and good compatibility, has become increasingly popular in medicine, especial y in orthopedics. At present, porous tantalum is used in the preparation of the integrated acetabular cup, total hip arthroplasty acetabular cup, acetabular reinforcing pad, porous tantalum metal rod, tibial plateau prosthesis and patel ar prosthesis and tibial plateau prosthesis. The short-term fol ow-up of porous tantalum implants in orthopedic surgery has been reported. And clinical data, radiology, histological analysis of the removed material have proved the practicability and safety of the porous tantalum. But the long-term effects of porous tantalum remain to be confirmed.

Objective To investigate the short-term therapeutic effect of liraglutide injection combined with insulin pump subcutaneous infusion of insulin in the treatment of early-onset obesity.Methods 100 patients with diabetes mellitus treated with endocrine specialist outpatients from May 2015 to August 2016 in our hospital were selected and randomly divided into three groups,insulin+liraglutide injection 1.2 mg/d group(treatment group 1),insulin+metformin 0.5 tid group(treatment group 2)and insulin intensive group(control group).The control group of 20 cases,the remaining 40 cases each group.Each patient was treated with their own treatment regimen.Target blood glucose: fasting blood glucose≤7.0mmol/L,2h postprandial blood glucose≤10mmol/L that is compliance,blood glucose≤4.4mmol/L that low,blood glucose≤3.9mmol/L that low blood sugar.Two groups of patients adhere to three months of treatment.At the time of admission,after 2 weeks of treatment and 6 months of treatment,venous blood was taken for biochemical examination.The corresponding biochemical indicators were compared and the therapeutic effects of the two regimens were compared.Results There were significant differences in the levels of fasting glucagon,fasting C-peptide and body mass index between the two groups after three weeks of treatment and three months after treatment(P<0.05).The total amount of insulin,the time of reaching the standard and the frequency of hypoglycemia of three groups were compared,the difference was statistically significant(P<0.05).Conclusion Using three kinds of treatment for the treatment of elderly obese diabetesmellitus have a significant effect.However,the treatment regimen of insulin+liraglutideinjection 1.2 mg/d is effective in the control of diabetes and the total insulin dosage,as well as the number of hypoglycemia occurred,are better than the other two groups of treatment.