Objective To study the genetic toxicity of surfactants Tween-20?Tween-80?Span-20?BE-2. Methods To take root tip cells of Vicia faba as the subjects, treated with various concentrations of surfactants to study the effect of them on the micronucleus and chromosome aberration in root tip cells of Vicia faba. Results The frequencies of micronucleus cell induced by surfactants Tween-20?Tween-80?Span-20?BE-2 were (21.34?1.31)‰, (13.44?2.66)‰, (15.29?3.16)‰ and (7.53?2.34)‰ respectively, the frequencies of chromosome aberration induced by surfactants Tween-20?Tween-80?Span-20?BE-2 were (18.16?0.50)%, (11.05?1.18)%, (23.79?1.62)% and (22.58?0.61)%, significant differences were seen compared with the control. Conclusion The results of the present paper demonstrates that surfactants Tween-20?Tween-80?Span-20?BE-2 have the effect of mutagenic agent.

Objective To study the changes of cerebral haemodynamics in neonates with hypoxic-ischemic encephalopathy(HIE) treated with Shengmai injection compared with citicoline,and explore the clinical interventions of Shengmai injection on HIE.Methods Fifty patients with HIE were randomized into 2 groups: shengmai group treated with Shengmai injection(n=30),and citicoline group treated with citicoline(n=20).The haemodynamic parameters included peak systolic flow velocity(PSFV),end-diastolic flow velocity(EDFV),pulsation index(PI) and resistance index(RI) of anterior and middle cerebral artery were detected by color Doppler ultrasound before treatment and on 7~(th) day after treatment respectively.Control group(n=50) compared with the two groups.Results PSFV,PDFV and RI of HIE patients were significantly lower than those of control group(P

Objective: To optimize matrix composition and technology for Jiefushuang Emulsion. Methods: Orthogonal design was used, oil phase, agitation velocity, emulsifiers and emulsify temperature were selected as variable factors. Results: The optimum matrix composition and technology were: The ratio of oil to water was 2∶3, emulsifiers: 320g(oil 500mL), HLB: 15, emulsity temperature: 70?C , agitation velocity: 800 revolutions per minute, lasting for 20 minutes. Conclusion: The emulsion prepared accords with the stipulation of the appendix of Chinese pharmacopoeia (2000).

Objective To explore the distribution and genotypes of plasmid-mediated quionlone resistance (PMQR)genes and intI1 integrase genes in Enterobacteriaceae isolates.Methods The PMQR genes and intI1 integrase genes were identified by polymerase chain reaction in the nonduplicate strains of E.coli (80),E.cloacae (18)and K.pneunoniae (27).The positive PCR products were subj ect to DNA sequencing analysis.The gene-positive strains were tested by conj ugation experiment.The minimum inhibitory concentrations (MICs)of donor,recipient strains and transconj ugants were tested by agar dilution method with quinolones and other antimicrobial agents.Results Sixteen (12.8%)of the 125 Enterobacteriaceae isolates were qnr gene positive,including 8 qnrS1 positive and 8 qnrB6 positive.Furthermore,the aac(6′)-Ib-cr gene was identified in 15 (12.0%) strains.Twenty PMQR-positive isolates harbored intI1 integrase gene.The conjugation experiments were successful in 12 of the 26 PMQR-positive isolates and 7 of the 20 intI1-positive isolates.The MICs of quinolones and other antimicrobial agents against the transconj ugants were higher than the MIC values against recipient strains.Conclusions The PMQR genes are prevalent in Enterobacteriaceae isolates.The PMQR-positive isolates can co-harbor integrase genes.These resistance genes have the feature of horizontal transfer,to which close attention should be paid.

Objective To test the content of menthol in Chuanbei Pipa Syrup(CPS)by GC, and to evaluate its quality. Meth-ods The content of menthol in CPS was determined by capillary gas chromatography. DB-WAX capillary column (30 m ×0. 319 mm × 0. 50μm) was used with nitrogen as carrier gas. The precolumn pressure was 500 kPa and the column temperature was at 110 ℃ with split ratio of 25 : 1. The temperature was set at 250℃ for sample injection and for the detector. Results The content of menthol in CPS produced by Henan Pharmaceutical Co. , Ltd. , Zhengzhou Pharmaceutical Co. , Ltd. and Yunan Pharmaceutical Co. , Ltd. was 0. 154 mg/mL, 0. 203 mg/mL and 0. 109 mg/mL, respectively. Of them, the men-thol content in CPS produced by Zhengzhou Pharmaceutical Co. , Ltd. met the requirements of Pharmacopoeia(2005 edition), and that of the other two species was slightly lower. Conclusion The methods are convenient, reliable and accurate for the de-termination of menthol content in Chuanbei Pipa Syrup.

Objective To study the burden of dementia patients' caregivers and related influencing elements.Methods A survey was carried out in 152 dementia patients' caregivers after studying the prevalence of dementia in Gushan town using self-designed questionnaires.Results Among the 152 earegivers,65.13% received primary school education or below.And those taking care of disabled dementia patients,taking care of patients with higher ADL scores,attending on without professional helper,having insufficient knowledge,reluctant to give care,and spending much time on it got higher burden score.Conclusions Among caregivers of dementia patients,females are more than males;sons,daughters and spouse are the primary caregivers.Their education level is low,and the caregivers don't have enough knowledge on dementia care and can't give enough service to the senile dementia patients.

Objective To assess the value of acute physiology and chronic health evaluationⅡ (APACHE-Ⅱ) score and extrapancreatic inflammation on abdominal computed tomography (EPIC) score in predicting outcome of patients with acute pancreatitis (AP).Methods One hundred and forty-seven patients with AP who underwent abdominal computed tomography scan within 24 h and acute physiological examination after admission were studied.To assess their value in predicting severe AP and complications,the APACHE Ⅱ score and EPIC score was calculated and evaluated by calculating receiver operator characteristic (ROC)curves,the sensitivity,the specificity,the Youden index and the area under the ROC curve (AUC).Results Forty-three of 147 patients developed severe AP.The mean scores of APACHE-Ⅱ score and EPIC score two criteria systems was significantly higher in severe AP than that in mild AP (P< 0.01).EPIC score had 0.893 sensitivity,0.870 specificity and the largest AUC for predicting severe AP.EPIC score had the largest AUC in predicting local complications.But the EPIC score was equal to the APACHE-Ⅱ score in AUC to predict systemic complications.Conclusion In patients with AP.EPIC score and APACHE-Ⅱ score combination will estimate the prognosis of disease accurately within 24 h of admission.

Objective To study the risk factors which lead to increase in the incidence rate of hypospadias. Methods A case-control design was used.The mothers of 210 cases and 420 controls were interviewed by telephone or face-to-face,and the questionnaires were answered.Thirty probable risk factors were investigated.Statistical analysis was performed using univariate and multivariate unconditional Logistic regression analyses for evaluation of the data. Results All boys with hypospadias in the case group were of Han nationality.Only 5 cases had positive family history.Odds ratio (OR) of the factors investigated were:pregnant age (OR,38.63),threatened abortion (OR,4.71),progestin (OR,3.64),estrogen (OR,5.1),pesticide (OR,2.39),organic solvents (OR,2.12),season of pregnancy (OR,1.86) and detergent (OR, 1.87).But OR of frequency of abortion,weeks of gestation and birth weight were less than 1.The results of other 17 investigated factors were negative. Conclusions Threatened abortion,nonfull-term fetus,low birth weight and some environmental endocrine-disturbing chemicals ( pesticide,detergent,organic solvents,progestin and estrogen) may be risk factors associated with hypospadias.These environmental endocrine-disturbing chemicals may increase the incidence rate of hypospadias.

0.99. The recovery rates were 72.25% -109.0% with the relative standard deviation (RSD) of 1.82% -11.61% . Conclusion The method is fast,simple,sensitive and needs no organic solvent and it is applicable to the determination of organochlorine pesticides and chlorobenzene compounds in water.

0.05).Pain-disappearing time,anus-bubbling time,and time for gas-fluid plane disappearing were shortened in group C,and drainage volume from nutrient canal during gastrointestinal decompression obviously reduced on the second day as compared with those in groups A and B(P