1.Perception Gap of Medical Personnel Service Quality by Servqual Method
Modern Hospital 2016;16(1):86-88,89
Objective To enhance the hospital′s competitiveness through evaluating hospital by its own staff.Methods The Servqual was used to investigate the hospital service quality.Results The hospital′s medical staff were good in personal appearance and professional knowledge.However, the hospital was week in equipment and fulfilling promises to patients.Conclusion The administrative should take corresponding measures to improve the quality of medical services for their existing management quality problems.
2.Advance of Research for Effects of Heat Wave on Health of Urban Residents and Warning
Journal of Environment and Health 2007;0(08):-
In recent years,high-temperature heat wave caused an increase in the number of deaths than other climate disasters,the study of the effects of heat waves on the health of urban residents health becomes a subject of concern.Sustained high-temperature heat waves will inevitably cause harm to human health,to understand the characteristics of heat waves is conducive to the prediction of high temperature heat waves and to the establishment of reasonable response measures.This paper mainly introduces the present status of heat wave occur,the impact of heat wave on human health,the influencing factors for excess mortality during heat waves as well as the research methods for heat wave which are commonly used to predict.
4.Heat Wave and Daily Mortality of Residents in a Coastal City in China
Heng CHEN ; Liping LI ; Yingning CHEN
Journal of Environment and Health 2007;0(11):-
Objective To understand the effects of heat wave on daily mortality of residents in a coastal city in China.Methods In this paper,semi-parametric generalized additive model was used to study the relationship between average daily temperature and the daily mortality of local residents,and every day lag on the impact of daily mortality was observed by the distributed lag model,2005 -2007.Results The relation between the coastal city daily mortality and the daily average temperature was fitted,2005-2007.The relation between them was approximately "V".In summer,the daily average temperature increased 1℃,the relative risk factor increased 0.036.In the establishment of the semi-parametric generalized additive regression model,the daily average temperature and daily mortality showed a significant linear relationship(P
5.Signal transduction mechanisms underlying ultraviolet-induced inflammatory responses in keratinocytes
Chinese Journal of Dermatology 2016;49(2):140-143
Ultraviolet (UV) is a kind of important environmental factor that can cause dermatitis,photoaging and skin cancerization.The UV-induced production of inflammatory factors by epidermal keratinocytes has been deemed an important molecular event in occurrence of the above diseases.Various receptors,intracellular signal transduction-related regulatory molecules and nuclear transcription factors are involved in UV-induced inflammatory responses in keratinocytes,which mainly include epidermal growth factor receptor,aryl hydrocarbon receptor,peroxisome proliferatoractivated receptor,protein kinase C family,protein kinase B,mitogen-activated protein kinase family,nuclear factor-κB and transcription factor activator protein-l,etc,and constitute a complex inflammatory signal transduction network.
6.AXON-PROJECTION OF ENDOMORPHIN-2-CONTAINING NEURONS IN THE TRIGEMINAL GANGLION TO THE MEDULLARY DORSAL HORN IN THE RAT
Heng LIAO ; Tao CHEN ; Yunqing LI
Acta Anatomica Sinica 1954;0(02):-
Objective To observe whether endomorphin-2-immunoreactive(EM2-ir) neurons in the trigeminal ganglion(TG) project to the medullary dorsal horn(MDH) in the rat. Methods Fluoro-gold(FG) retrograde tracing and immunofluorescent histochemical staining for EM2 were applied. Results After FG was injected into the MDH,many FG labeled neurons with small,medium and large diameters were observed in the TG.There were also aboundant EM2-ir neurons with different diameters in the TG.The majority of FG labeled neurons in the TG showed EM2-immunoreactivities.Almost all of the EM2/FG double-labeled neurons were medium and small in size.Conclusion EM2-ir neurons in the TG project to the MDH in the rat.
7.Therapeutic effect of pericarpium zanthoxylum oil on experimental hyperlipemia in mice
Liang HENG ; Chen LI ; Min JIA
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
Objective To observe the therapeutic effect of pericarpium zanthoxylum oil on experimental hyperlipemia in mice. Methods Hyperlipemia model was reproduced in mice, and then pericarpium zanthoxylum oil was garaged for 4 weeks. Blood samples were obtained from retro-ocular vessel. The levels of blood lipids were determined, the hemorrheology parameters were determined by hemorrheometer, and the pathological changes in the liver of mice were observed microscopically. Results Zanthoxylum oil could obviously reduce contents of TC, TG, LDL-C (P
8.Effect of resveratrol on apoptosis and cell cycle of human keratinocytes irradiated by ultraviolet B
Hongying CHEN ; Xu CHEN ; Li LI ; Heng GU
Chinese Journal of Dermatology 2021;54(5):408-413
Objective:To evaluate the effect of resveratrol on apoptosis and cell cycle of human epidermal keratinocytes (HEKs) after ultraviolet B (UVB) irradiation.Methods:After 12-hour treatment with 0 (control group) , 25, 50, 100, 150 and 200 μmol/L resveratrol, cell counting kit-8 (CCK8) assay, bromodeoxyuridine (BrdU) assay and lactate dehydrogenase (LDH) assay were performed to evaluate the effect of resveratrol on proliferative activity and death of HEKs. Some HEKs were divided into 4 groups: normal control group receiving no treatment, resveratrol group treated with 100 μmol/L resveratrol, UVB group irradiated with 50 mJ/cm 2 UVB, and UVB+resveratrol group irradiated with 50 mJ/cm 2 UVB followed by 12-hour treatment with 100 μmol/L resveratrol. Western blot analysis was conducted to determine the expression of apoptosis-related proteins poly (ADP-ribose) polymerase (PARP) and caspase-3, as well as cell cycle-related proteins cyclin B1 and cyclin E1, and flow cytometry to detect the apoptosis and determine cell cycle distribution. One-way analysis of variance was used for comparisons among multiple groups, and least significant difference- t test for multiple comparisons. Results:The proliferative activity of HEKs significantly differed among the 25-, 50-, 100-, 150-, 200-μmol/L resveratrol groups and control group ( F=46.52, P < 0.001) , and was significantly lower in these resveratrol groups than in the control group (all P < 0.001) ; the DNA synthesis activity of HEKs was significantly lower in the 100-μmol/L resveratrol group (0.761 ± 0.141) than in the control group (2.226 ± 0.141, t=17.92, P < 0.001) ; there was no significant difference in cell death rate among the 25-, 50-, 100- and 200-μmol/L resveratrol groups ( F=1.938, P > 0.05) . After treatment with or without UVB and/or resveratrol, there were significant differences in the apoptosis rate, proportion of cells at G1, G2 and S phases, expression of PARP, caspase-3, cyclin B1 and cyclin E1 among the 4 groups (all P < 0.05) . The UVB group showed significantly increased apoptosis rates (24.69% ± 3.54%) and cleavage levels of PARP and caspase-3 (2.190 ± 0.349, 0.090 ± 0.016, respectively) compared with the normal control group and UVB+resveratrol group (4.00% ± 0.81%, 0.926 ± 0.040, 0.024 ± 0.019, respectively, all P < 0.05) ; the UVB group showed significantly decreased protein expression of cyclin E1 and cyclin B1 (0.116 ± 0.017, 0.775 ± 0.080, respectively) compared with the normal control group, and the UVB+resveratrol group showed significantly increased expression of cyclin E1 (0.287 ± 0.047) , but decreased expression of cyclin B1 (0.504 ± 0.009) compared with the UVB group (all P < 0.05) ; the UVB group showed significantly decreased proportion of S-phase cells, but increased proportion of G1- and G2-phase cells compared with the UVB+resveratrol group (all P < 0.05) . Conclusion:Resveratrol can decrease the proliferative activity of HEKs, inhibit apoptosis induced by UVB radiation, and regulate cell cycle progression.
9.Observe the effectiveness of ileal transposition for weight and blood glucose
Denggang CHEN ; Heng LI ; Jingli CAI ; Rong CHEN
International Journal of Surgery 2014;41(10):680-682,封3
Objective Through developing the experimental animal model of ileal transposition (IT) with GK-rats,to explore the effects of ileal transposition on water and food intake,weight change and blood glucose.Methods Thirty male GK-rats were randomly assigned to three groups:IT(n =10),C (n =10) and B (n =10)group.Rats in IT group underwent ileal transposition,and others underwent sham operation.Every group was given sufficient clean water.Water and food intake,weight change blood glucose and oral glucose tolerance test(OGTT) were recorded preoperatively and postoperatively.Results The total deaths of the experiment was four and the general survival rate was 88.6%.After operation,water intake reduced visibly in all groups,IT group was kept low level for a long time but not in C and B group.The food intake of IT group was lower than the other two groups during a certain period,but that phenomenon didnt last long.The weight change showed similarly to the food intake.The fasting plasma glucose(FPG) and OGTT were ameliorated significantly in IT group but not in B and C group.Conclusions It is feasible to develop ileal transposition animal models with GK-rats.Moreover,the author found that the influence of IT on weight in GK rats was transient,but on water intake and blood glucose was lasting.
10.Significance of Hypoxia Ischemia on Cerebral Cortex Injury and Neurofunctional Development in Immature Rats Brain
li-li, SONG ; zhi-heng, HUANG ; chao, CHEN
Journal of Applied Clinical Pediatrics 2006;0(15):-
Objective To establish brain hypoxic-ischemic (HI) model using postnatal day 3(P3) SD rats and evaluate the apoptotic neuronal cells in cerebral cortex and neurofunctional development.Methods The P3 rats were randomly divided into HI group (n=35) and control group(n=18).HI was induced in P3 rats with right carotid artery ligation followed by 2.5 h hypoxia in 60 mL?L-1 oxygen at 37 ℃.The injury of neural cells in the cerebral cortex was evaluated by tumor necrosis factor receptor-1(TNF-R1),Caspase-3 immunostaining and Hematoxylin-Eosin (HE) staining in 24 h and 7 d after HI,respectively.Furthermore,the neurofunctional development was evaluated by negative geotaxis reflex and eye opening time.The data were analyzed by SPSS 12.0 software.Results Caspase-3 and TNF-R1 positive cells were abundant in the ipsilateral cortex at 24 h after HI,compared with contralateral part and control group(P