MicroRNAs (miRNAs) are a class of small,non-coding,single-stand RNA molecules which are composed of 21-25 ribonucleotides.They regulate gene expression at post-transcription and participate in many important biological processes including cell growth,apoptosis,viral infection and cancer development.At present,the studies on miRNAs in cervical lesion include miRNAs expression characteristic of cervical cancer and precancerous lesion,the association with HPV infection and prognosis of cervical lesion,cervical cancer development,diagnosis and therapy.

Objective To explore the application value of enzyme linked immunosorbent assay for detection of hepatitis B PreS1 antigen and antibody in the diagnosis of hepatitis B.MethodsSeveral common patterns in the results of hepatitis Btwo halfcheck were selected from August 2014 to August 2016 for the sample, the use of hepatitis Btwo half7 commonly used detection model for 140 cases of PreS1 antigen, antibody detection.Enzyme linked immunosorbent assay for detection of PreS1 antigen and antibody.ResultsIn patients with HBeAg in PreS1 antigen positive rate was 87.5%, in patients with positive PreS1 antigen positive rate was 8.6%, HBsAg, HBcAb in patients with positive PreS1 antigen positive 13.3%, in a pure HBsAg in patients with positive PreS1 antigen 4.3%.There was no significant difference in the total positive rate of HBV-DNA and the total positive rate of PreS1, and the total coincidence rate was higher.PreS1 antigen positive correlated with HBsAg, HBeAg and HBcAb positive(P<0.05), and has nothing to do with HBsAb, HBeAb positive.ConclusionHepatitis B PreS1 antigen and antibody detection, can effectively complement the diagnosis of hepatitis B, early detection of hepatitis B infection.

Objective To observe the clinical effects of compound carraghenates cream on edema and pain after mixed hemorrhoid surgery. Methods 180 cases of patients with mixed hemorrhoids divided into the observation group and the control group, 90 cases in each group according to the random number table method. The control group was treated with Vaseline oil gauze to cover wounds after operation, and the observation group was treated with compound carraghenates cream after operation. The clinical efficacy, VAS pain score , the edema symptom scores and the pain relief time, edema symptom relief time, the wound healing time ,untoward effect were compared and observed after treatment between the two groups. Results The total effective rate of treatment in the observation group was significantly higher than that in the control group (95.56％ vs 85.56％) (P<0.05). The VAS pain scores of the observation group on the 1st day, 3rd day and 7th day after treatment were significantly lower than those of the control group (P<0.05), and the edema symptom scores on the 3rd day and 7th day after treatment were significantly lower than those of the control group (P<0.05). The time for pain relief, the time for edema relief and the wound healing time in the observation group were significantly shorter than those in the control group (P<0.05). Conclusion Compound carraghenates cream can effectively relieve edema and pain after mixed hemorrhoid surgery, and it also can shorten the course of disease and promote wound healing with safety.

To investigate the effects of pyrrolidine dithiocarbamate (PDTC) on the proliferation and PCNA (proliferating cell nuclear antigen) expression of cultured human retinal pigment epithelium cells, human retinal pigment epithelium cells (RPE) were cultured from normal adults who, died accidentally. The effects of PDTC on the proliferation of RPE cells were examined by using methyl thiazlyl tetrazolium (MTT) assay. The effects of PDTC on the PCNA expression of RPE cells were immunohistochemically examined by employing biological image analysis system (BIAS). After treatment with PDTC of various of concentration ranging from 0.062 to 1 g/L for 24 h, or concentrations ranging from 0.031 to 1 g/L, the proliferation of RPE cells decreased in a dose-dependent manner. After treatment with PDTC of concentration varying from 0.062 to 1 g/L for 24 h, the PCNA expression was also suppressed in a dose-dependent manner. It is concluded that PDTC can inhibit the proliferation of RPE cells in vitro in a dose-and time-dependent manner, at least in part, by down-regulating the expression of PCNA. PDTC may be used to prevent and treat the proliferative vitreoretinopathy (PVR).

Objective To investigate the influence of surface grafting density of polyethylene glycol (PEG) molecules on the interactions of nanoparticle with the biomembrane.Methods The dynamics of polymer as well as its shielding effect on the wrap of the membrane were investigated using coarse-grained (CG) molecular dynamics simulations.A coarse grained model force field,MARTINI,was employed for its ability to reproduce experimental properties of various lipid dynamics and some kinds of polymer as well.Results As a result,structural variations and energy changes of grafted PEG molecules on the nanoparticles,adsorbing dynamics of PEGs on the membrane as well as the wrap rates of the membrane were obtained to shed light on the PEGylation-mediated shielding mechanism in the adsorption of PEGylated nanoparticles on the membrane.Conclusions The surface grafting density of PEGylated nanoparicles has been found to play a crucial role in the stealth shielding behavior of PEGylated nanocarriers.These studies are consistent with experimental observations and to some extent give a molecular level interpretation of the macroscale observations of prolonged circulation half-life of stealth nanocarriers.

Objective To explore the role of halofuginone in immune regulation,observe the effects of halofuginone on maturation of dendritic cells and immune tolerance during homograft heart transplantation of rats,in order to provide new ways and means to treat transplantation tolerance and to explore its possible mechanism.Methods Rat bone marrow derived dendritic cells were isolated and the effects of halofuginone in LPS-treated DCs were investigated by flow cytometry;heart transplantation model of rats were established and the effect of halofuginone stimulated dendritic cells in the immune tolerance by pathological analysis and mixed lymphocyte reaction were observed.Results Flow cytometry results show that the expression of cell surface CD80 CD86,OX62 antigens were not high in control group,their expression increased significantly when stimulated by LPS for 24h,and decreased when stimulated by both Changshan ketone and LPS for 24h,and even lower than their expression levels in blank group,which showed that Changshan ketone has the ability to inhibit the maturation of dendritic cells induced by LPS.In rat heart transplantation model,dendritic cells stimulated with Changshan were injected into recipient rats in vivo,and receptor of rat transplantation rejection decreased significantly,which showed that dendritic cells stimulated with Changshan ketone may be used as a new method for cell therapy in treatment of transplant rejection.Conclusion This study systematically observes that halofuginone can inhibit the maturation of rat bone marrow-derived dendritic cells and lymphocyte proliferation for the first time in vivo and in vitro.

On-body communication channels are a physical basis for the establishment of an effective and reliable wireless link between on-body nodes in wireless body area networks (WBANs).Due to normally strict limitations in transmit power and power consumptions of on-body nodes as well as the human-centered characteristics of WBANs,on-body channels are prone to be affected by the electromagnetic properties of human tissues,human movements,application environments and transceiver antennas,which increase greatly the complexity of the research on channel models.The paper discusses the developments,mathematical expressions and application characteristics of five types of on-body channel models,such as the simple path loss model,the hybrid path loss model,the small-scale fading model,the scenario-based model and the ultra-wideband model,and reviews the current progress on the modeling on-body channels.

ObjectiveTo investigate the clinicopathologic features of lymphoblastic lymphoma (LBL).Methods21 cases of LBL were examined by light microscopy and immunohistochemistry,and analyzed with clinical features.Results21 cases of LBL patients ranging in age from 4 to 86 years old,including 17 cases originated from the lymph nodes, the other 4 were from the nasopharynx, breast, pleura and mediastinum respectively. Histopathologically: lymphoid cells were median sized, showing a diffuse infiltration. The neoplastic cells had fine chromatin, higer nuclei mitoses. The tumors were positive for TdT and CD99.ConculsionPatients especially who have enlarged lymph nodes in adolescents favor the diagnosis of LBL.The tumor cells show a diffuse infiltration,usually with median size,fine chromatin.The positivity for TdT in immunostaining can confirm the diagnosis of LBL.

Some basic knowledge of the outbreak and direction of earthquake, and shows that in some areas the medical resources are wasted severely are briefly introduced. A long-term mechanism and emergency draft be set up for antiearthquake and rescue is suggested, which should aim at different areas and districts manage in large scale and reasonably distribute the limited medical resources. In this way, the indemnification can be raised, the victims' life and property can be saved, and the disaster areas can obtain better service.

OBJECTIVE To improve efficiency of hospital infection administration and take strict precautions against medical malpractice in Chinese level Ⅱ hospital of UN Peacekeeping Army in Lebanon.METHODS The current situations of its hospital infection administration were enumerated,compared and analyzed,and corresponding measures of administration were suggested.RESULTS Under the influence of characters of medical missions during peacekeeping,the hospital infection administration presented the problems that patients and disease spectra were diversified,the rate of hospital infection was influenced by peacekeeping mission,the positive rate of HIV was high,medical workers protection awareness was lagged behind,and the disposition of hospital was illegitimate;many measures should be taken to enhance the administration level.CONCLUSIONS In order to well develop the hospital infection administration in Chinese level Ⅱ hospital of UN Peacekeeping Army in Lebanon,we should reform and improve the system of hospital infection administration,and establish an effective and systematic prevention system of hospital infection.