A total of one hundred and sixty rats were fed with isothiocyanatelabelled candida albicans and then randomly divided into four groups: simple burn (30% TBSA full-thickness scald burn was created on the back), simple antibiotic therapy (oxacillin combind with gentamicin were injected intraperitineally), 30% full-thickness burn plus antibiotic therapy and the control group. The animals were sacrificed and examined at different time phases. The labelled candida albicans were traced in the mesentery lymph nodes, liver, spleen, kidney, lung, blood stream and urine. The labelled candida albicans could be demontrated from 30% of visceral organs at one hour and from 70% at six hours after burn plus antibiotic therapy, while the mesentery lymph node and liver were the primary organs invaded. The process of candida translocation through the intestinal wall was displayed as adhesion and then invasion. For comprative study of the methods or the early diagnosis of systemic dissemination of candida albicans, blood cultures, urine cultures and direct examination of urine under microscope were performed. The detectable rate was only 8% in blood culture and 21.8% in urine culture, however, it was 40% in direct examination of urine, the later method is therefore much more sensitive than the other two.

OBJECTIVE: To study the drug resistance of pseudomonas aeruginosa so as to provide references for rational use of antibiotics in the clinic.METHODS: The clinical distribution and the susceptibility test results of pseudomonas aeruginosa isolated from Beijing Tiantan Hospital in 2006 were analyzed retrospectively.RESULTS: The resistance rate of 334 strains to imipenem stood at 39.8%.The pseudomonas aeruginosa strains were mainly isolated from three important departments(neurological,respiratory and neurosurgery),the resistance rates to imipenem in the above three departments in descending order were as follows: department of respiratory(48.3%),department of neurological(40.2%),and neurosurgery(17.0%).CONCLUSION: The Pseudomonas aeruginosa isolated from Beijing Tiantan Hospital were highly resist to imipenem reduced and the strains isolated from different department varied in bacterial resistance,suggesting the drug resistance of antimicrobial surveillance should be emphasized in some key departments to keep the drug resistance under control.

This paper introduces the operational principle and structure of laser camera. The adjustment of image gray- scale and density is also mentioned

This paper discusses the limitations of traditional evaluation standard for digital medical images, and points out the reasons to select DQE as the new one. The differences of the two standards are compared and analyzed.

Objective:To observe influence ofβreceptor blocker on heart rate variability (HRV)after military train-ing in soldiers.Methods:A total of 80 soldiers with heart rate>60 beats/min from a group army were selected,and randomly divided into metoprolol group (n=40)and control group (n=40).Metoprolol group received metoprolol succinate (12.5mg,once/d)continuously before exercise 5d,while control group received placebo of same dose, 5min dynamic electrocardiography was used to analyze HRV.Heart rate,blood pressure and HRV changes were compared between two groups before and after 5km running.Results:There were no significant difference in heart rate,blood pressure and HRV indexes between two groups before exercise;compared with control group after exer-cise,heart rate and blood pressure were lower,and there were significant reductions in standard deviation of normal to normal RR intervals calculated over the 24 h period [SDNN,(80.1±9.5)ms vs.(69.4±10.5)ms],standard deviation of normal to normal RR intervals in all 5 min segments of the entire recording [SDANN,(65.7±7.8)ms vs.(60.1±9.2)ms],SDNN index [(25.1±11.3)ms vs.(20.4±12.3)ms],root-mean square of differences be-tween successive normal to normal intervals [rMSSD,(25.2±10.7)ms vs.(19.9±11.3)ms],very low frequency [VLF,(451.1±312.9)ms2 vs.(408.3±299.8)ms2 ],low frequency [LF,(191.0±79.3)ms2 vs.(148.2±77.4) ms2]and high frequency [HF,(69.6±50.1)ms2 vs.(51.8±52.7)ms2]in metoprolol group,P<0.05 all.Con-clusion:βreceptor blocker usage before exersise can reduce heart rate variability change after military training,and is helpful to decrease occurrence of acute cardiovascular events.

Objective To explore the possible mechanism of acute cerebral hemorrhage with systemic inflammatory response syndrome (SIRS) which caused multiple organ dysfunction syndrome (MODS), and to investigate the significance of serum nitric oxide (NO) and nitric oxide synthase (NOS) in the development of acute cerebral hemorrhage from SIRS to MODS.Methods The incidence rate of acute cerebral hemorrhage leading to SIRS and MODS was investigated. Serum NO and NOS levels in 73 patients with acute cerebral hemorrhage were detected dynamically by nitratase and chromometry methods. 20 normal healthy people were adopted as control group.Results The incidence rate of cerebral hemorrhage accompanied with SIRS was 47.95%(35/73),74.29%(26/35) of which leaded to MODS. 73 patients serum NO and NOS levels were elevated obviously, and the differences were significant compared with control group (all P

Objective To study X-ray and CT diagnosis and Differential diagnosis of nonossifying fibroma.Methods 29 cases of nonossifying fibroma proved by pathology were included in this paper.29 cases underwent radiography and 12 cases underwent CT scan.There were 22 male and 7 female,with age ranged from 5~59 years(mean age 21.3 years),X-ray and CT findings of tumors were analysed.Results The tumors were located in long bone in 27 cases,in which 17 cases were located in metaphses,2 cases in diaphyses,8 cases in bone end and 2 cases in irregular bone.The maximum diameter of tumors ranged from 0.5~7 cm.The tumors were round,oral or mild lobular in 21 cases,the tumors were showed sclerotic borders in 26 cases.Septum was demonstrated in 22 cases on X-ray plain films,but only 2 cases on CT.Bone expansion was showed in 13 cases.Conclusion Most of nonssifying fibroma have characteristic X-ray and CT findings,the diagnosis be identified by X-ray and CT before operation.

To study the role of the reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) gene and matrix metalloproteinase-2 (MMP-2) in the regulation of trophoblast invasion of early pregnancy. Immunohistochemistry, Western blot and gelatin zymography were used to detect the RECK protein expression localization, expression level and MMP-2 activation level in the placental tissues harvested from 52 normal pregnant women (27 in the early pregnancy, 25 in the term pregnancy). Immunohistochemistry showed that RECK expression was found both in villous tissues of early pregnancy group and term pregnancy group and was mainly observed in cell membrane and cytoplasm of cytotrophoblasts and syneytiotrophoblasts. RECK expression increased with gestational time. RECK expression of early pregnancy group was significantly lower than that of term pregnancy group (P<0.05). RECK expression was significantly lower in cellular column (CC) with invasion ability. Western blot showed that the RECK protein expression in early pregnancy group was significantly lower than that in term pregnancy (P<0.05). The optical density values of RECK protein expression in early pregnancy group and term pregnancy group were 1.35-0.14 and 2.68+/-0.26, respectively, while MMP-2 activation ratio was contrary to RECK protein expression and decreased with the gestation time (P<0.01). The MMP-2 activation ratios of early pregnancy group and term pregnancy group were 0.46 +/- 0.05 and 0.10+/-0.02, respectively. The expression of the tumor inhibitory gene RECK was positively related with the invasion ability of trophoblasts, while the invasion gene MMP-2 was negatively related with the ability. The interaction between RECK and MMP-2 may play an important role in the regulation of the trophoblast invasion in early pregnancy.

Standardized training of resident doctors is the golden stage for clinicians to establish good clinical thinking. Although respiratory disease is common, there are serious and critical diseases, which need to develop clinical thinking and improve the analytical ability. The resident physicians' instructor should help residents to establish the correct diagnostic thinking through the following means: clinical demonstration teaching show, guidance of reading the relevant literature and books, strengthening the basic skills of collecting patients' history and physical examination, and guidance of appropriate use of auxiliary examination and analysis of the results. For the treatment thinking, solid theoretical knowledge and life-long learning ideas and habits can improve the successful rate of initial treatment. Furthermore, being familiar with the efficacy evaluation system and analyzing the reasons for the success and failure of the treatment will help the residents to develop a good therapeutic thinking. The good clinical thinking established by the residents in the department of respiration will be conducive to their own follow-up specialist training and career development.

Objective To evaluate the effects of restraint stress on behaviors of mice with focal cerebral ischemia. Methods Male mice were first subjected to left middle cerebral artery occlusion/reperfusion. Repeated restraint stress (2 hours/day for 2 weeks) was given from 1 week after surgery. At the end of stress scheme, sucrose solution consumption test, open-field test and forced swimming test were performed. Results Spontaneous locomotor activity and coordination test were decreased to the lowest level on the 1st day after MCAO compared with the baseline values before surgery (P