In this paper, it has been found that such stressors as operation or swimming in cold watermay suppress the mitogenic response of murine spleen cells to ConA or LPS, whichis closely related to the action of suppressor cells.Using the plastic-adherence method, the plastic-adherence cells in spleen(PAC)was removed or obtained. The difference of mitogenic responsivebefore and after the removal of PAC is very apparent in stressed mice, being much lower thannormal before the renioval of PAC and near to normal after removing PAC. It is interestingthat the lymphocyte transformation stimulated by LPS is suppressed predominantly after swim-ming stress instead of those by ConA in operation stress.It has also been observed that theindomethacin may block the induction of suppressor PAC by stress, which means that the pro-staglandins may play an important role in the induction and action of suppressor PAC.

Using hybridoma technique,17 hybridoma cell lines against astrocyte were ob-tained.The screening test of McAb showed:(1)Using immune enzymatic histochemistryfixed cell staining method all of 17 McAb reacted with astrocyte,(2) Using immuneenzymatic histochemistry viable cell staining method,eight of seventeen McAb react-ed with astrocyte.As to biological properties of McAb results were as follows:(1)Seven of eight McAb that showed positive reaction in viable staining assay have di-fferent degree of cytotoxicity to astrocyte,thymocyte and spleen cell in presence ofcomplement,one of eight has specific cytotoxicity only to astrocyte.(2) McAb(2C)possesses blocking effect on astrocyte,thymocyte and spleen cell proliferation respon-sive to various kind of mitogen.Furthmore,it has been found LPS may promote theproliferation of astrocytes and secreting interleukin 1(IL-1)-like substance This IL-1like substance seems to have a mutiple biological activities,i.e:(1)Thymocyte prolife-ration.(2) Fibroblast(L929 cell) proliferation and(3) Endogenous pyrogen activities.

Cultivation of murine thymic epithelium has been established.Murine thymic epi-thelial cultural supernatant showed activity increasing both spontaneous ~3H-TdR inco-rporation and ConA-stimulated proliferation of thymocytes by the exponent of 1.5-2at dilution 1:8 to 1:32,the peak of the activity was present at 10-20 or 15 days ofcultivation.However,the thymic epithelial supernatant from cultures at 6-7 daysshowed activity suppressing both proliferations of thymocytes at dilution 1:8-1:16.Theresult showed thymic epithelial supernatant had no 1L-2 activity.

In this paper, it was reported that rat thymocytes harbor both high and low affinity ACTHR. The proper number of thymocytes used for deterting ACTHR varies from 1?10~6 /0.2 ml to 2 ?10~6 /ml, the maximum binding of ACTH to ACTHR occurs at 20 minutes. The IC_(50) for ACTH concentration is 800 nM or so. The saturation curves show that thymocyte ACTHRs are saturated at the concentration of 2.5 nM of ~(125)IACTH. There are high affinity ACTHR of 840 sites/cell on thymocyte (KD=0.15nM, Bmax = 2. 8 fmol/2 ? 10~6 cells); whereas low affinity ACTHRs are 12400 sites/cell (KD=4.2 nM, Bmax = 41.5 fmol/2 ? 10~6 cells).Moreover amputation stress can reduce thymocyte ACTHR remarkably.

Objective:To investigate the changes of Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues of patients with heptocellular carcinoma and its clinical significance.Methods:Th1/Th2 subsets and cellular factor were detected by ELISPOT and double-sandwich ELISA.Results:Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues of patients with heptocellular carcinoma,Th1 sebset expressing cellular factor IFN-?,IL-2,IL-12 were significantly lower,and Th2 sebset expressing cellular factor IL-4,IL-10 were significantly higher than those of the normal control group.PMBC in patients with heptocellular carcinoma producing Th1 sebset cytokins deduced by PHA and IL-12 were significantly higher and producing Th2 sebset cytokins were significantly lower than those in the three month after operation.Conclusion:Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues were of imbalance and immune functional status of patients with hepatocellular carcinoma was inhibitory.Therefore,it was nessassory to enhance immune functional status of patients during treatment.

Objective:To evaluate the effect of Helicobacter pylori infection on hyperdynamic circulatory state and portal hypertensive gastrropathy in liver cirrhotic patients.Methods:Helicobacter pylori infection,IL-1,IL-8,TNF-?,Endotoxin,NO,ET,anti-VacA and anti-CagA levels were determined in cirrhotic patients by using rapid urease test,()~(14)C-urea expiratory test,immunoimpression test,nitrite colorimetric test and ELISA.The parameters of portal venous system flow were determined by doppler ultrasonography.Results:HP prevalence in patients with liver cirrhosis was 77.1%,anti-VacA and antiCagA positive rate was 60.4% in common.IL-1,IL-8,TNF-?,Endotoxin,NO and ET levels in anti-VacA and anti-CagA positive group were significantly higher than in anti-VacA and anti-CagA negtive group.The amount of splenic venous flow(SVF) and superior mesenteric venous flow(SMVF)in anti-VacA and anti-CagA positive group was greater and the amount of portal venous flow(PVF) was smaller than those in negtive group.Conclusion:There was the effect of Helicobacter pylori infection on hyperdynamic circulatory state and portal hypertensive gastrropathy in liver cirrhotic patients.

OBJECTIVE:To establish a HPLC method for the determination of main component and the related substances in pirenoxinum natricum eye drops. METHODS: A Inertsil C8-3 column was used with pH2.2 perchloric acid-methanol (35∶65) as mobile phase at a flow of 1.0 mL?min-1. The detection wavelength was 230 nm and the sample size was 10 ?L. RESULTS: Pirenoxinum natricum and the related substances could be baseline separated. The calibration curve was linear in the range of 49.5～990 ng(r=0.999 9). The detection limits was 89.3 pg, and average recovery was 99.1%, RSD was 0.42%. CONCLUSIONS: This method is rapid, accurate sensitive and can be used for the quality control of pirenoxinum natricum eye drops.

Objective: Our purpose was to study the predictive factors of interferon(IFN) therapeutic effectiveness. Methods:Genotypes of HCV, HCV RNA quantities, β2 microglobulin, 2′- 5′oligoadenylate synthetase, and peripheral blood lymohocyte subgroup were detected by using specific primer PCR assay, energy transference technique of signal primer, sheet chromatography, radioimmunoassay, and APAAP immunoenzyme bridge technique respectively in 20 blood samples of patients with chronic hepatitis C. Results:(1)The patients who had lower level of HCV RNA before treatment and had continuing decreased HCV RNA level after treatment had good response to IFN treatment in follow-up survey. (2)The patients with HCV-Ⅲ type had complete response much more than those with HCV-Ⅱ type (P＜0.05).(3)The patients with lower level of 2′- 5′oligoadenylate synthetase before treatment might have good response to IFN treatment. (4) There was no significant change in β2 microglobulin level before and after treatment and in different response groups before treatment.(5) The CD+3, CD+4, and CD+4/CD+8 in peripheral blood increased after treatment. The patients in complete response group had higher level CD+3, CD+4, and CD+4/CD+8 than those in part response and non-response groups, but there was no significance in statisitcs. Conclusion:The HCV RNA quantities, HCV genotypes, 2′- 5′oligoadenylate synthetase level before treatment were predictive factors of IFN therapeutic effectiveness. The changes of HCV RNA level in treatment and follow-up survey had important significance in therapeutic evaluation, β2 microglobulin level, and lymphocyte subgroup before treatment were not used as predictive factors for IFN therapeutic effectiveness.

Objective To explore the effect of spa with free position on parturition of primipara.Methods One hundred and thirty-eight primiparas were equally randomized into control group and observation group. The control group conducted Doula delivery,which means a delivery at recumbent or semi-recumbent position when mouth of the uterus opened by 3 cm and the primiparas in the observation group took free position with spa based on Doula delivery. The two groups were compared in terms of the durations of each labor and total labor, postpartum bleeding 2 h after delivery and neonatal Apgar score,delivery way,pains,anxiety and feeling of control at delivery.Results The postpartum bleeding in the observation group was significantly lower than that in the control group(P<0.05). The durations of first labor, second labor and total labor in the observation group were all significantly shorter than those of the control. The degrees of pain and anxiety were lower than those of the control group,while the control sense at delivery was better than that of the control group(P<0.05,for all). There were no differences in the rate of spontaneous delivery,the rate of uterine-incision delivery,the rate of episiotomy and scores on 1 min Apgar scales(P>0.05).Conclusion Aided delivery by spa with free position can reduce labor pain and anxiety levels,improve the birth control and comfort and ultimately enhance the sense of delivery control and comfort.

Objective To compare the mRNA expressions of interleukin (IL)-17 and IL-23 in peripheral blood of patients with psoriasis vulgaris versus healthy individuals,assess the relationship of these parameters with psoriasis area and severity index (PASI) score,and to investigate the therapeutic mechanisms of total glucosides of peony (TGP) for psoriasis vulgaris.Methods Fifty patients with psoriasis vulgaris and 40 healthy individuals were enrolled in this study.Of these patients,42 were treated with TGP of 600-900 mg twice a day for 8 weeks.Blood samples were obtained from all the healthy individuals,50 patients before treatment,42 patients after 4-week treatment,and 23 patients after 8-week treatment.Real-time fluorescence-based quantitative reverse transcription PCR (RT-PCR) was used to determine the mRNA expression levels of IL-17 and IL-23 in the blood samples.The severity of psoriasis was evaluated using PASI score before and after the treatment.Statistical analysis was done by t test,rank sum test,and Pearson correlation analysis using the SPSS16.0 software.Results The IL-17 and IL-23 mRNA expression levels (given in △Ct value) in the patients before treatment were significantly higher than those in the healthy controls (IL-17,-5.32 ± 0.80 vs.2.79 ± 0.76,t =47.71,P ＜ 0.05; IL-23,-5.43 ± 0.68 vs.-3.77 ± 0.86,t =10.38,P ＜ 0.05),and positively correlated with the PASI score (r =0.61,0.52 respectively,both P ＜ 0.05).A significant decrease was observed in the mRNA expression levels of IL-17 and IL-23 as well as PASI score in the 42 patients after 4-week treatment with TGP compared with those before treatment(IL-17,-2.24 ± 0.61 vs.-5.30 ± 0.78,t =20.40,P ＜ 0.05; IL-23,-1.97 ± 0.74 vs.-5.44 ± 0.68,t =21.69,P ＜ 0.05; PASI,5.8 ± 2.7 vs.9.4 ± 4.2,t =4.68,P ＜ 0.05),and in the 23 patients after 8-week treatment compared with those after 4-wek treatment(IL-17,-1.51 ± 0.78 vs.-2.21 ± 0.59,t =3.50,P ＜ 0.05; IL-23,-1.27 ± 0.81 vs.-1.89 ± 0.72,t =2.70,P＜ 0.05; PASI,3.8 ± 1.8 vs.7.3 ± 2.5,t =5.47,P＜ 0.05).Conclusions It seems that both IL-17 and IL-23 are involved in the pathogenesis of psoriasis vulgaris,and TGP treatment can reduce the mRNA expression levels of IL-17 and IL-23 as well as PASI score in patients with psoriasis vulgaris.