1.Experimental study of gut absorptive capacity after trauma detected by D-xylose content test with micromethod
Chinese Journal of Tissue Engineering Research 2003;7(20):2810-2811
Aim To establish a rapid and sensitive diagnostic markerfor adjusting gut absorptive capacity after trauma. Methods A mi-cromethod for D-xylose test with phloroglucinal was established according toEberts with modification. The standard curves were repeated for fivetimes. Then the blood contents of fasting and orally taking 0. 5 g/kgD-xylose at 2nd and 4th hour were measured and the blood D-xylose con-tents of seven refilling rats with ischemia of intestine were mea-sured. Results The maximal absorption spectra was read at 554 nm byscanming of DU-7 Beckman. Good linearity of the D-xylose standard curvewas found with the range of 0 to 4 mmol/L(r=0. 9979 ±0. 0017) . Theblood content of rats at 2nd hour after orally taking D-xylose was ( 154 ± 6)mg/L amd that at 4th hour was decreased to (87 ± 11) mg/L. And theblood content at 2nd and 4th day after ischemic refilling was (162 ± 5)mg/L and (80 ± 8) mg/L respectively. The variation coefficients within andamong batches were 1.98% (n=6) and 3.10% (n=6), respective-ly. And D-xylose recovery rates were from 97.2% to 104. 3%. ConclusionMicromethod for D-xylose test appears to be simple, rapid and sensitive,and is an available index for estimating intestinal absorption after severetrauma.
2.The Physical Chemistry Properties Evaluation of Copying and Original Aluminium Phosphate Gel
Dan MEI ; Dakui LI ; Peidong SUN
China Pharmacy 2001;0(11):-
OBJECTIVE:To compare the physical chemistry properties of copying(A) and original(B) aluminium phosphate gel METHODS:We examined the character,antacid activity,isolating capacity against H+ ions and viscosity in different pH values of two products RESULTS:Product A was not better than product B in the Character,antacid activity,isolating capacity against H+ ions and viscosity;The antacid activity of two preparations not influenced obviously by the food with low concentration;Two preparations all had difference physical chemistry properties in batches CONCLUSION:Product B was better than A in comparison of the physical chemistry properties
3.Effect of chronic fluorosis on myoardial collagen metabolism in rats
Dan LI ; Wen YANG ; Zhe FAN ; Bo SUN ; Guangsheng LI
Journal of Jilin University(Medicine Edition) 2006;0(01):-
0. 05). Conclusion Chronic fluorosis has no obvious effect on myocardial collagen metabolism of rats and myocardial collagen is not likely the main target of fluoride.
4.Comparision of Different Methods of Area Measurement in Irregular Scar
Dan RAN ; Wanjuan LI ; Quangang SUN ; Jianqiu LI ; Qing XIA
Journal of Forensic Medicine 2016;32(5):338-341
ObjectiveTo determine a measurement standard of irregular scar area by comparing the ad-vantages and disadvantages of different measurement methods in measuring same irregular scar area. MethodsIrregular scar area was scanned by digital scanning and measured by coordinate reading method, AutoCAD pixel method, Photoshop lasso pixel method, Photoshop magic bar filled pixel method and Foxit PDF reading software, and some aspects of these methods such as measurement time, repeata-bility, whether could be recorded and whether could be traced were compared and analyzed.Results There was no significant difference in the scar areas by the measurement methods above. However, there was statistical difference in the measurement time and repeatability by one or multi performers and only Foxit PDF reading software could be traced back.ConclusionThe methods above can be used for measuring scar area, but each one has its advantages and disadvantages. It is necessary to develop new measurement software for forensic identification.
5.Study on preparing Danshen Injection by micelle-ultrafiltration
Dan WANG ; Tao GONG ; Bing SUN ; Li LI ; Fangmi FU
Chinese Traditional Patent Medicine 1992;0(06):-
Objective: To prepare Danshen Injection (Radix Salviae Miltiorrhizae) by micelle-ultrafiltration. Methods: For preparing Danshen Injection, we packed the impurities in the macrofiltered liquor of Radix Salviae Miltiorrhizae with micelle of Tween-80, and ultrafiltered the compounds with ultrafiltration membrane withholding molecular weight more than 50000. Then we evaluated the process with the comprehensive indexes including loss rate of UV absorbance, loss rate of Protocatechualdehyde and the stability of transparence. Conclusion: The process of Danshen Injection prepared by micelle-ultrafiltration is better than that of ordinary ultrafiltration.
6.Ethical Thinking on Hospital Laboratory Information System
Dan SHEN ; Li SUN ; Haiju LIU ; Jiubo FAN ; Zhishan LI
Chinese Medical Ethics 2015;(4):611-614
This paper discussed the existing problems of Information System ( LIS) from six aspects, inclu-ding:the security and the expansion of the function, protection of patients′privacy, the establishment of emergency response mechanisms, authenticity and accuracy of data and the risks for auto-approval rules, etc.The authors also analysed the necessity of its rectification from the perspective of ethics, proposed improvement programme and suggestions: strengthening the safety management systems and data, ensuring special check the confidentiality of the project, protect the patient′s privacy;setting up emergency mechanism;hospital based on the actual workload and resources to determine the LIS function module; regularly checking the test data, to avoid occupational inju-ries;continuously improving the rigour of automatic approval rules.
7.Treatment experience of traumatic splentic rupture in 32 cases
Yong LI ; Renhua GONG ; Dengqun SUN ; Kailang LI ; Zhenyu DAN
International Journal of Surgery 2013;40(10):653-655
Objective To discuss the remedy and clinical effect of traumatic splentic rupture.Methods Clinical data of 32 cases of traumatic splentic rupture were retrospective analyzed.Results No dead cases,6 cases were cured by conservative treatment,18 cases were explored by laparoscope,and 14 cases remained spleen,4 cases were converted to traditionary splenectomy.Traditionary splenectomy was done on 8 cases directly.Conclusions Ⅰ,Ⅱ grade of traumatic splentic rupture may choose conservation treatment or remain spleen by laparoscope operation.Splenectomy should be done on Ⅲ,Ⅳ grade of traumatic splentic rupture in time.
8.Effect of progressive addition lens on peripheral refraction in adolescent myopic eye
Yan-li, SUN ; Dan-dan, LIN ; Hong-hui, LI ; Jia, QU ; Jun, JIANG
Chinese Journal of Experimental Ophthalmology 2012;30(9):834-838
Background Peripheral refraction of retina brings about influence on myopia development,and different corrective methods will play different roles on the peripheral refraction.Objective This study was to measure the peripheral refraction with progressive addition lenses (PALs) and single-vision spectacle lenses (SVLs) corrected in adolescent myopic eyes and to investigate the difference between them.Methods This was a selfcontrolled cross-sectional study.Forty corrected myopic eyes with PALs were recruited for the study.Twenty-three male and seventeen female subjects aged 10-15 years (mean 12.54 years±1.45 years) enrolled in this study.The refractive errors of the subjects ranged form-1.50 D to-6.00 D (mean-3.74 D±1.09 D) and the astigmatism was less than-1.50 D with the corrected visual acuity at least 20/25.1% Tropicamide eye drops was topically administered for the cycloplegia and then Grand Seiko WAM-5500 Auto-refractor was used to measure the peripheral refraction.The measured points were determined follows as:fovea (0°),peripheral temporal (T30°,T20°,T10°) and nasal (N30°,N20°,N10°) visual fields from the right eyes.The mean of the six measuring points for each position was calculated.Peripheral refraction was measured from each subject under the wearing of PALs or SVLs (randomly in succession),and the statistic analyse perimeters included M value,relative peripheral refractive error (RPRE),J45,J180.Written informed consent was obtained from each subject before examination.Results The corrected visual acuity was 4.98 ± 0.65 and 4.97 ± 0.53 under the PALs and SVLs conditions respectively,showing in significant difference between them (t =0.222,P =0.639).Hypermetropic shift in peripheral M value and RPRE value were found with the increase of visual angle field,in contrast,the peripheral J180 showed a myopic shift,and significant difference was found among the six peripheral points with all of the above three perimeters (P<0.05).Negative J45 values in the temporal visual filed and positive J45 values in the nasal filed showed less shift.Some peripheral angles of the peripheral refractions in M value,RPRE value,J180,J45 showed significantly difference from the fovea refraction(P<0.05).Compared with SVLs correction,PALs induced relatively myopic shifts in M value and RPRE value in all of the peripheral angle fields,showing significant differences(P<0.05).Conclusions PALs causes relatively myopic shift at the peripheral retina in adolescent myopic eyes in comparison with SVLs,and the important parameters are M value and RPRE value.The effect of PALs on controlling the onset and progression of myopia may be interpreted by peripheral refraction.
9.Study on Acupoint Selection Principles of Facial Paralysis Treatment for Acupuncture and Moxibustion Based on Ancient Literature
Dan LI ; Minghui XIA ; Jia DU ; Zhaosheng YAN ; Zhongren SUN
Chinese Journal of Information on Traditional Chinese Medicine 2015;22(8):47-49
Objective To investigate principles of acupoint selection for the ancient acupuncture and moxibustion treatment of facial paralysis.Methods The ancient literature about acupuncture and moxibustion was collected and reviewed from the pre-Qin period to the late Qing Dynasty. According to the data selection criteria, acupuncture and moxibustion prescriptions of facial paralysis were collected and input into an ancient acupuncture and moxibustion prescription database. By use of frequency analysis and factor analysis, the principles of acupoint selection were obtained.ResultsTotally 257 prescriptions were collected which involve 74 acupoints. Through frequency analysis, 19 acupoints were obtained whose frequencies were equal or greater than 9, such as Dicang (ST 4), Jiache (ST 6), Tinghui (GB 2), Shuigou (GV 26), Hegu (LI 4), etc. Through factor analysis of acupoint variables, 6 common factors were obtained.Conclusion Based on analysis of the common factors, the principles of acupoint selection are summarized which were according to meridian, part and etiology for the ancient acupuncture treatment of facial paralysis. The results could provide a reference for clinical acupuncture and moxibustion treatment. It is feasible that factor analysis was applied to study principles of acupoint selection for acupuncture and moxibustion prescriptions.
10.Protective effect of conjugated linoleic acid on vascular endothelial cells
Dan YI ; Guangyou LI ; Fayi LIU ; Xiuqin SUN
Chinese Journal of Marine Drugs 1994;0(01):-
Objective To investigate the effects of conjugated linoleic acid(CLA)on survival rate of vascular endothelial cells(VEC).Methods The survival rate of VEC was assessed using MTT method after treating with CLA.Apoptosis and necrosis of the cells were determined using annexin V-propidium iodine double staining.Results CLA significantly increased the cell survival rate to 126.3% after treating for 72h at a dose of 2?mol?L-1,survival in a time-and dose-dependent manner when the concentration was less than 5?mol?L-1.CLA could partly prevent the death of VEC induced by PA and SA.Conclusion CLA can partly prevent the death of VEC induced by PA and SA.The results suggest that CLA are important for the survival of vascular endothelial cells.