Objective:To investigate the relationship between poly(ADP-ribose)polymerase(PARP) inhibition and the growth activity of murine colon carcinoma CT26 cell lines in vitro.Methods:The murine colon carcinoma CT26 cell lines were treated with PARP inhibitior 5-AIQ in vitro.MTT assay was used to determine the growth activity of CT26 cells.The expressions of PARP and NF-?B p65 in the nuclei of CT26 cells were investigated by double labelling immunofluorescence and laser scanning cofocal microscope.Results:The inhibitory rates of the growth in 5-AIQ-treated colon carcinoma CT26 cell groups were 17.52%、27.63% and 39.93%,respectively.The inhibitory rate increased with the raising of the 5-AIQ concentration(0.1mmol/L,0.5mmol/L,and1.0mmol/L),and the difference was significant(P

The pathogenesis of barium-induced severe myocardial necrosis in dietary potassum-depleted rats was investigated. In this study four experiments were performed. The results of these experiments suggest that there exist a complex pathogenesis of myocardial lesion produced by barium in condition of potassium deficiency. The main mechanism seems to be the direct stimulating effect of barium on myocardial contractibility. It seems unlikely that barium-induced constriction of coronary arteries, hypertension, hypokalemia, and increased catecholamine secretion are the basic mechanism of the myocardial necrosis. But these factors may play a promoting or aggravating role in the development of the myocardial lesion. Potassium deficiency not only acts as a conditioning factor for barium-induced myocardial necrosis, but also relatively increases the cardiotoxity of barium. This may be the principal pathogenesis of severe myocardial necrosis produced by barium in combination with potassium deficiency.

The effects of nitrendipine (Nit) on myocardial contractility, myocardial succinate de-hydrogenase (SDH) and cytochrome oxidase (CCO) activities in streptozotocin (65mg/kg) diabetic rats were studied. Four weeks after the induction of diabetes, the rats were treated with Nit (30mg ?kg -2/day) for 4 weeks. The results showed that ventricular diastolic function was affected after 4 weeks of diabetes, and both ventricular diastolic and systolic functions were obviously involved after 8 weeks. These changes were significantly improved in diabetic rats receiving Nit treatment. The myocardial SDH and CCO activities in diabetic animals were markedly lower as compared to controls. The attenuation of these enzyme activities in diabetic rats was significantly reversed by administration of Nit. These findings suggest that Nit treatment may exhibit some beneficial effects on diabetic cardiomyopathy.

ObjectiveTo investigate the effect of down-regulating epidermal growth factor receptor (EGFR) expression on Bleomycin induced pulmonary fibrosis in mice.MethodsForty 4 ～6 week aged C57BL/c male mice were randomly divided into control,bleomycin,bleomycin plus EGFR RNAi groups and RNAi negative control group.Bleomycin group were treated with bleomycin (3 mg/kg) by endotracheal injection on day 0,control group were treated with PBS.And bleomycin plus EGFR RNAi group were received EGRF siRNA plus bleomycin intratracheal administration.RNAi negative control group received negative EGRF siRNA plus bleomycin intratracheal administration.Mice were sacrificed 10 days after the treatments.Hydroxyproline (HYP) assay was performed in the lung tissue.The lung tissue slides were examined pathologically with H.E.staining,and EGFR mRNA expression was detected by reverse transcriptasepolymerase chain reaction (RT-PCR) technique.Western blot were performed to identify the protein level.of total EGFR and phosphorylated EGFR.ResultsHistological examination of lung specimens demonstrated that EGFR siRNA administration lessened lung fibrosis induced by bleomycin and significantly reduced HYP content (543.00±25.89 vs 900.73±31.77,P＜0.01).EGFR mRNA (0.31±0.05 vs 0.75±0.08,P ＜0.01) and protein expression(1.53±0.47 vs 2.56±0.37,P ＜0.01) in EGFR siRNA-treated mice was significantly decreased.The expression of phosphorylation of EGFR protein (1.78±0.35 vs 2.84±0.51,P ＜0.01) and EGFR protein in RNAi group was less than in bleomycin group.Conclusions EGFR RNAi reduced the BLM-induced lung fibrosis by inhabiting EGFR activation.

Oxidative stress plays an important role in the progression of diabetes mellitus. As a potent antioxidant, alpha-lipoic acid is able to clear free radicals and alleviate oxidative damages and therefore has been widely applied in the clinical management of diabetes mellitus. This article summarizes the clinical application of alpha-lipoic acid in alleviating diabetes mellitus-related oxidative damages, protecting vascular lesions, treating diabetic polyneuropathies, and modulating insulin sensitivity.

Sysmex CA-1500 is used for in vitro diagnostic fully automatic blood coagulation analyzer, can rapid precision analysis batch sample. It is the use of condensation, yield color and immunoassay methods to detect and analyze data, and the result can be saved, display and print. This article mainly introduces Sysmex CA-1500 Full-Automatic Coagulation Analyzer analysis and fault field analysis and in the use process, share met fault point inspection analysis, for their peers to provide a platform.

Objective To explore the relevance between writing characteristic and therapeutic effect in schizophrenia and to discuss the influence of aggressive behavior on writing characteristic. Methods Re-coding the casual and fixed writing in admission, one week, two weeks, four weeks, eight weeks after treatment and rating Positive and Negative Syndrome Scale (PANSS) and Modified Overt Aggression Scale (MOAS). Choosing two characteristics, “relationship between font and grid lines” and “having big strokes or not”, and comparing before and after treatment. Results Eight weeks after treatment, the score of PANSS decreased. The condition of patients and the writing characteristic improved as well. The differ-ences of writing characteristics were statistically significant in patients with aggressive behavior before and after treatment (P<0.05). Conclusion The writing characteristic has relation with therapeutic effects and improved with therapeutic effects in aggressive patients.

Objective To understand the level of tulnor necrosis factor-α(TNF-α)and asymmetric dimethylarginine (ADMA) in serum of healthy adults and primary hypertension patients with different left ventricle,and to explore the role of TNF-α and ADMA in the pathophysiological mechanism of heart damage due to hypertension.Methods Thirty health adults were recruited as control.Based on the left ventricular mass index(LVMI)and relative wall thickness(RWT),66 patients of hypertension were divided into 4 groups:normal left ventricle group with normal LVMI and RWT,concentric remolding group with normal LVMI and incremental RWT,typical concentric hypertrophy group with incremental LVMI and RWT,eccentric left ventricular group incremental LVMI and normal RWT.TNF-α was measured by radioimmunoassay and ADMA was measured through reversed phase high-performance liquid chromatography.Resuits TNF-α and ADMA increased with the severity of the left ventricle.Each left ventricle group was significantly higher than control group (P<0.01).The differences between left ventricle groups were statistically significant (P<0.01).Conclusions TNF-α and ADMA may participate in the development of left ventricle.