1. The internal iliac artery was investigated in 100 cases. In 23 cases this arterywas divided into anterior and posterior trunks, while in the remaining 77 cases suchtrunks were absent. 2. The mode of origin of the superior, inferior gluteal arteries and the internalpudendal artery from the internal iliac artery, may be classified into five principal typesand four subtypes. Among them the second type is most frequent (54 cases), the firstand the third type are next in order, their occurrence are 24 cases and 17 cases respec-tively. The fifth type possesses 2 common trunks and occurs in 4 cases. 3. The obturator artery, according to its origin, is divided into three types. Thefirst type, arises from the internal iliac artery, comprises the largest number (81 cases).The second type, arises from the external iliac artery, is next in number (15 cases). Thethird type has two obturator arteries arising from the internal and external arteries (4cases) respectively. 4. The iliolumbar artery arises from the internal iliac artery (54 cases), some fromthe superior gluteal artery (12 cases). 5. The lateral sacral artery, mostly arises from the superior gluteal artery, occursfrom 1--3 branches. There are 2 branches in 43 cases, one branch in 32 and threebranches in 25 cases.

Child ; Child, Preschool ; Drug Therapy ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Remission Induction ; methods ; Survival Analysis ; Treatment Outcome

Objective:To observe the effects of double-stranded small interfering RNA (siRNA) of the silent mating-type infor-mation regulation 2 homolog 1 (SIRT1) on the cell proliferation, cell cycle progression, and expression levels of the cell cycle negative regulators. These regulators include P21, P27, and phosphorylated retinoblastoma (PRb) proteins present in prostate cancer PC3 cells. This work further aims to explore the possible underlying mechanism for such effects. Methods:PC3 cells were cultured in vitro and then randomly divided into the mock group, scramble siRNA transfected group, and SIRT1 siRNA-transfected group. SIRT1 siRNA ef-ficiency was examined through reverse transcription polymerase chain reaction and Western blot analysis. The inhibitory rate of PC3 cell growth was determined through a methyl thiazolyl tetrazolium assay, and the cell cycle was investigated with the use of flow cytom-etry. The P21 and P27 protein expression levels and PRb status were determined by Western blot assay. Results:Compared with those of the mock and scramble siRNA groups, the expression levels of SIRT1 mRNA and protein significantly decreased in SIRT1 siR-NA-transfected cells. In addition, the inhibitory rate of PC3 cell growth was markedly increased, and the cell cycle of the PC3 cells was arrested at the G1 stage. The expression levels of negative cell cycle regulators, including P21 and P27 protein levels increased, whereas Rb protein phosphorylation was inhibited in SIRT1 siRNA-transfected PC3 cells. Conclusion: SIRT1 RNA interference inhibits PC3 cell growth and arrests cell cycle progression through the upregulation of the P21 and P27 proteins and the inhibition of Rb protein phosphorylation.

ObjectiveTo seek an effective protocol fortreating spinal cord injury-induced limb dysfunction by comparing the therapeutic effects of electroacupuncture at Huatuo jiaji points plus Du meridian points versus conventional electroacupuncture. MethodForty patients with spinal cord injury-induced limb dysfunction were allocated, using a random number table, to treatment and control groups, 20 cases each. Both groups were treated with ganglioside and routine rehabilitation therapy. In addition,the treatment group received electroacupuncture at Huatuo jiaji points and Du meridian points and the control group, conventional acupuncture. Lower limb function was assessed using ASIA Motor Score, Sensory Score and the MBI in the two groups of patients before treatment and after one and two courses. The clinical therapeutic effects were evaluated.ResultThere was a statistically significant pre-/post-treatment difference in lower limb dysfunction in both treatment and control groups (P<0.05). The improving effect was better in the treatment group than in the control group (P<0.05,P<0.01).ConclusionElectroacupuncture at Huatuo jiaji points and Du meridian points has a definite therapeutic effect on spinal cord injury-induced limb dysfunction. It is superior to conventional electroacupuncture.

Objective To study CT findings of sacral tuberculosis.Methods CT findings of sacral tuberculosis confirmed pathologically in 13 cases were analysed combined with literature review.Results The S 1～2 tuberculosis was most common.The types of sacrum destruction were osteolytic and bone destruction along the sacral foramen.The cold abscess were distributed at the presacral,unilateral iliac fossa and/or the gluteal region.Three patients(23.1%) were associated with sacroiliac joint tuberculosis(right 2,left 1).Conclusion In the patients with sacral tuberculosis the patterns of sacrum destruction,region of cold abscess and its relationship with the neighboring structures can be well showed by CT,and it can help surgeons to choose the appropriate surgical approach.

Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.

Central Nervous System ; pathology ; Central Nervous System Neoplasms ; drug therapy ; Child ; Child, Preschool ; Humans ; Methotrexate ; therapeutic use ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy

Objective To elucidate the relationship between liver mitochondria calcium content and respi- ratory function in obstructive jaundice and biliary drainage.Methods The model of obstructive jaundice and bil- iary drainage was produced in dogs by common bile duct ligation and choledochoduodenostomy.The liver mitochon- dria were isolated and the calcium content and respiratory control rate were monitored.Results Mitochondria res- piratory control rate and calcium content significantly decreased and elevated respectively after bile duct ligation and there were varying degrees of recovery after biliary drainage,and the degree of recovery was evidently correlated with the duration of obstruction before drainage and the time after drainage.There was a strong negative correlation between the mitochondria calcium content and change of respiratory function.Conclusion The mitochondria cal- cium overload may be one of important factors for suppressed mitochondrial respiration in obstructive jaundice and early relief of the obstruction could favour the recovery of damaged liver mitochondria.

Streptococcus pneumoniae is one of the most common cause of invasive disease,such as bacteremia,meningitis,and empyema,et al.But there has significant difference on the incidence of invasive pneumococcal disease among different regions and differernt groups of people.In addition the severity and mortality of invasive pneumococcal disease are closely related to the changes of serotypes,virulence of streptococcus pneumoniae and also the human immune response.The pneumococcal vaccination is an important measure to prevent streptococcus pneumoniae infection,providing good protection to vaccinees and createing herd immunity effect.This article briefly describes the pathogenesis,risk factors and preventive strategies of invasive pneumococcal disease.

AIM: To observe the effect on visual quality by traditional Chinese medicine (TCM) warm-reinforcing method for early idiopathic macular epiretinal membrane(IMEM).METHODS: Totally 21 patients (21 eyes) diagnosed as early IMEM with spleen-kidney yang deficiency were treated by Gubenmingmu powder for 2mo.The best corrected visual acuity(BCVA), the contrast sensitivity and the modified vision quality of questionnaire score were detected to compare the life influence of pretherapy with posttherapy in visual changes.RESULTS: The BCVA were separately 0.24±0.07、0.22±0.06、0.27±0.08 in the treating time of 1, 2 and 5mo.Contrast sensitivity function in special frequencies of 6,12 and 18c/d increased than pretherapy (P<0.01), those of 3c/d were no statistically significant (P>0.05).Scales of night fixation difficulty, guidepost difficulty, TV font difficulty, satisfaction, annoyance, understanding, newspaper easiness had significant differences(P<0.01), while the rest of the scales had no significant differences(P>0.05).CONCLUSION: The TCM warm-reinforcing method could improve the visual function of IMEM patients and improve patients` quality of life.