1. The internal iliac artery was investigated in 100 cases. In 23 cases this arterywas divided into anterior and posterior trunks, while in the remaining 77 cases suchtrunks were absent. 2. The mode of origin of the superior, inferior gluteal arteries and the internalpudendal artery from the internal iliac artery, may be classified into five principal typesand four subtypes. Among them the second type is most frequent (54 cases), the firstand the third type are next in order, their occurrence are 24 cases and 17 cases respec-tively. The fifth type possesses 2 common trunks and occurs in 4 cases. 3. The obturator artery, according to its origin, is divided into three types. Thefirst type, arises from the internal iliac artery, comprises the largest number (81 cases).The second type, arises from the external iliac artery, is next in number (15 cases). Thethird type has two obturator arteries arising from the internal and external arteries (4cases) respectively. 4. The iliolumbar artery arises from the internal iliac artery (54 cases), some fromthe superior gluteal artery (12 cases). 5. The lateral sacral artery, mostly arises from the superior gluteal artery, occursfrom 1--3 branches. There are 2 branches in 43 cases, one branch in 32 and threebranches in 25 cases.

Objective To study CT findings of sacral tuberculosis.Methods CT findings of sacral tuberculosis confirmed pathologically in 13 cases were analysed combined with literature review.Results The S 1～2 tuberculosis was most common.The types of sacrum destruction were osteolytic and bone destruction along the sacral foramen.The cold abscess were distributed at the presacral,unilateral iliac fossa and/or the gluteal region.Three patients(23.1%) were associated with sacroiliac joint tuberculosis(right 2,left 1).Conclusion In the patients with sacral tuberculosis the patterns of sacrum destruction,region of cold abscess and its relationship with the neighboring structures can be well showed by CT,and it can help surgeons to choose the appropriate surgical approach.

ObjectiveTo seek an effective protocol fortreating spinal cord injury-induced limb dysfunction by comparing the therapeutic effects of electroacupuncture at Huatuo jiaji points plus Du meridian points versus conventional electroacupuncture. MethodForty patients with spinal cord injury-induced limb dysfunction were allocated, using a random number table, to treatment and control groups, 20 cases each. Both groups were treated with ganglioside and routine rehabilitation therapy. In addition,the treatment group received electroacupuncture at Huatuo jiaji points and Du meridian points and the control group, conventional acupuncture. Lower limb function was assessed using ASIA Motor Score, Sensory Score and the MBI in the two groups of patients before treatment and after one and two courses. The clinical therapeutic effects were evaluated.ResultThere was a statistically significant pre-/post-treatment difference in lower limb dysfunction in both treatment and control groups (P<0.05). The improving effect was better in the treatment group than in the control group (P<0.05,P<0.01).ConclusionElectroacupuncture at Huatuo jiaji points and Du meridian points has a definite therapeutic effect on spinal cord injury-induced limb dysfunction. It is superior to conventional electroacupuncture.

Child ; Child, Preschool ; Drug Therapy ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Remission Induction ; methods ; Survival Analysis ; Treatment Outcome

Objective:To observe the effects of double-stranded small interfering RNA (siRNA) of the silent mating-type infor-mation regulation 2 homolog 1 (SIRT1) on the cell proliferation, cell cycle progression, and expression levels of the cell cycle negative regulators. These regulators include P21, P27, and phosphorylated retinoblastoma (PRb) proteins present in prostate cancer PC3 cells. This work further aims to explore the possible underlying mechanism for such effects. Methods:PC3 cells were cultured in vitro and then randomly divided into the mock group, scramble siRNA transfected group, and SIRT1 siRNA-transfected group. SIRT1 siRNA ef-ficiency was examined through reverse transcription polymerase chain reaction and Western blot analysis. The inhibitory rate of PC3 cell growth was determined through a methyl thiazolyl tetrazolium assay, and the cell cycle was investigated with the use of flow cytom-etry. The P21 and P27 protein expression levels and PRb status were determined by Western blot assay. Results:Compared with those of the mock and scramble siRNA groups, the expression levels of SIRT1 mRNA and protein significantly decreased in SIRT1 siR-NA-transfected cells. In addition, the inhibitory rate of PC3 cell growth was markedly increased, and the cell cycle of the PC3 cells was arrested at the G1 stage. The expression levels of negative cell cycle regulators, including P21 and P27 protein levels increased, whereas Rb protein phosphorylation was inhibited in SIRT1 siRNA-transfected PC3 cells. Conclusion: SIRT1 RNA interference inhibits PC3 cell growth and arrests cell cycle progression through the upregulation of the P21 and P27 proteins and the inhibition of Rb protein phosphorylation.

Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.

Central Nervous System ; pathology ; Central Nervous System Neoplasms ; drug therapy ; Child ; Child, Preschool ; Humans ; Methotrexate ; therapeutic use ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy

Objective To investigate if diastolic function could be improved by cardiac sympathetic nerve blockade in patients with dilated cardiomyopathy (DCM). Methods Sixty consecutive cases of DCM were randomed into therapeutic group and control group. Patients in therapeutic group were administered thoracic epidural blockade (TEB) (T 1-5 ) with 0.5 % lidocaine intermittent injection every two hours for four weeks, in addition to some routine medicine, while patients in control group were only administered routine treatment. Then the parameters of left ventricular diastolic function were evaluated with Doppler echocardiography before and after four weeks. Results ①All the patients in TEB group felt better within five minutes after injection of lidocaine within local epidural cavity.Their symptoms were relieved more rapidly than those of the drug therapy alone. Some patients with NYHA class Ⅳ could lie down supine in a short time. Exercise tolerance increased in patients with NYHA class Ⅱ to Ⅲ. But symptoms and signs of patients in control group were relieved very slowly, some patients even got worsen. ② Thirteen of thirty in therapeutic group got improvement on diastolic function after treatment. One of thirty in this group deteriorated. In detail, six of fifteen patients with demonstrated improvement of left ventricular diastolic filling changed from having a restrictive filling pattern to having a pseudonormal left ventricular filling pattern. Four of nine patients with a pseudonormal filling pattern changed to have a delayed filling pattern.Three of six patients, left ventricular filling pattern moved from a pseudonormal left ventricular filling pattern to a normal filling pattern. Only one patient acquired a restrictive filling pattern from a pseudonormal filling pattern. In contrast, in control group one of thirty got improvement on diastolic function after treatment. Nine of thirty in this group deteriorated. Individual changes of left ventricular filling pattern showed that only one patient improved from a delayed filling pattern to normal filling pattern, whereas seven patients had a restrictive left ventricular filling pattern developed from a pseudonormal filling pattern and another two patients achieved a pseudonormal filling pattern from delayed filling pattern. Conclusions Diastolic function in patients with DCM was improved significantly by cardiac sympathetic nerve blockade. The approach has beneficial effects on the patients of DCM.

OBJECTIVE:To evaluate the economics of penehyclidine hydrochloride and atropine in the treatment of acute organophosphorus poisoning (AOPP).METHODS:The information of 118 AOPP patients were collected and divided into group A (59 cases) and B (59 cases) according to therapy plan.There were 22 cases of mild poisoning,20 cases of moderate poisoning and 17 cases of severe poisoning in group A.There were 21 mild cases,21 moderate cases and 17 severe cases in group B.Based on routine treatment,group A was given Penehyclidine hydrochloride injection intramuscularly with initial dose of 1 mg (mild),2 mg (moderate) and 4 mg (severe).Group B was given Atropine sulfate injection intravenously,with initial dose of 2 mg (mild),5 mg (moderate) and 10 mg (severe).Both received maintenance treatment according to patients condition and stopped taking medicine after symptoms disappeared.Clinical efficacies,the time of acetylcholinesterase recovery and ADR were observed in 2 groups.The economics of therapy plans for mild,moderate and severe poisoning were evaluated in 2 groups by cost-effectiveness analysis.RESULTS:There was no statistical significance in total response rate of mild poisoning or the time of acetylcholinesterase recovery between 2 groups (P＞0.05).Total effective rates of moderate and severe poisoning in group A were significantly higher than group B,and the time of acetylcholinesterase recovery was significantly shorter than group B,with statistical significance (P＜0.05).There was no statistical significance in the incidence of ADR in mild,moderate and severe poisoning patients (P＞0.05).Cost-effectiveness ratio of penehyclidine hydrochloride was similar to that of atropine in mild poisoning patients;that of penehyclidine hydrochloride were significantly lower than that of atropine in moderate and severe patients.It was inline with the results of sensitivity analysis.CONCLUSIONS:Based on routine treatment,penehyclidine hydrochloride is similar to atropine in therapeutic efficacy of AOPP and the time of acetylcholinesterase recovery.For moderate and severe AOPP patients,penehyclidine hydrochloride is significantly better than atropine in improving therapeutic efficacy and the time of acetylcholinesterase recovery.The safety of 2 drugs are satisfactory;penehyclidine hydrochloride possesses cost-effectiveness advantage.

Long-term prognosis of stroke rehabilitation is associated with a number of factors, such as age, sex, race, lesion sites and sides, lesion nature and various complications. Under-standing these related factors not only contribute to clinical judgement of prognosis, but also provide the basis for the targeted rehabilitation therapy.