The pathogenesis of barium-induced severe myocardial necrosis in dietary potassum-depleted rats was investigated. In this study four experiments were performed. The results of these experiments suggest that there exist a complex pathogenesis of myocardial lesion produced by barium in condition of potassium deficiency. The main mechanism seems to be the direct stimulating effect of barium on myocardial contractibility. It seems unlikely that barium-induced constriction of coronary arteries, hypertension, hypokalemia, and increased catecholamine secretion are the basic mechanism of the myocardial necrosis. But these factors may play a promoting or aggravating role in the development of the myocardial lesion. Potassium deficiency not only acts as a conditioning factor for barium-induced myocardial necrosis, but also relatively increases the cardiotoxity of barium. This may be the principal pathogenesis of severe myocardial necrosis produced by barium in combination with potassium deficiency.

Objective:To investigate the relationship between poly(ADP-ribose)polymerase(PARP) inhibition and the growth activity of murine colon carcinoma CT26 cell lines in vitro.Methods:The murine colon carcinoma CT26 cell lines were treated with PARP inhibitior 5-AIQ in vitro.MTT assay was used to determine the growth activity of CT26 cells.The expressions of PARP and NF-?B p65 in the nuclei of CT26 cells were investigated by double labelling immunofluorescence and laser scanning cofocal microscope.Results:The inhibitory rates of the growth in 5-AIQ-treated colon carcinoma CT26 cell groups were 17.52%、27.63% and 39.93%,respectively.The inhibitory rate increased with the raising of the 5-AIQ concentration(0.1mmol/L,0.5mmol/L,and1.0mmol/L),and the difference was significant(P

The effects of nitrendipine (Nit) on myocardial contractility, myocardial succinate de-hydrogenase (SDH) and cytochrome oxidase (CCO) activities in streptozotocin (65mg/kg) diabetic rats were studied. Four weeks after the induction of diabetes, the rats were treated with Nit (30mg ?kg -2/day) for 4 weeks. The results showed that ventricular diastolic function was affected after 4 weeks of diabetes, and both ventricular diastolic and systolic functions were obviously involved after 8 weeks. These changes were significantly improved in diabetic rats receiving Nit treatment. The myocardial SDH and CCO activities in diabetic animals were markedly lower as compared to controls. The attenuation of these enzyme activities in diabetic rats was significantly reversed by administration of Nit. These findings suggest that Nit treatment may exhibit some beneficial effects on diabetic cardiomyopathy.

ObjectiveTo investigate the effect of down-regulating epidermal growth factor receptor (EGFR) expression on Bleomycin induced pulmonary fibrosis in mice.MethodsForty 4 ～6 week aged C57BL/c male mice were randomly divided into control,bleomycin,bleomycin plus EGFR RNAi groups and RNAi negative control group.Bleomycin group were treated with bleomycin (3 mg/kg) by endotracheal injection on day 0,control group were treated with PBS.And bleomycin plus EGFR RNAi group were received EGRF siRNA plus bleomycin intratracheal administration.RNAi negative control group received negative EGRF siRNA plus bleomycin intratracheal administration.Mice were sacrificed 10 days after the treatments.Hydroxyproline (HYP) assay was performed in the lung tissue.The lung tissue slides were examined pathologically with H.E.staining,and EGFR mRNA expression was detected by reverse transcriptasepolymerase chain reaction (RT-PCR) technique.Western blot were performed to identify the protein level.of total EGFR and phosphorylated EGFR.ResultsHistological examination of lung specimens demonstrated that EGFR siRNA administration lessened lung fibrosis induced by bleomycin and significantly reduced HYP content (543.00±25.89 vs 900.73±31.77,P＜0.01).EGFR mRNA (0.31±0.05 vs 0.75±0.08,P ＜0.01) and protein expression(1.53±0.47 vs 2.56±0.37,P ＜0.01) in EGFR siRNA-treated mice was significantly decreased.The expression of phosphorylation of EGFR protein (1.78±0.35 vs 2.84±0.51,P ＜0.01) and EGFR protein in RNAi group was less than in bleomycin group.Conclusions EGFR RNAi reduced the BLM-induced lung fibrosis by inhabiting EGFR activation.

During transportation of patients with cerebral infarction and OSAHS, 54 Patients in comparison group was not in the ambulance with ventilator while 73 patients in observation group with ventilation. After two weeks treatment, the observation group's heart rate, systolic blood pressure, diastolic blood pressure and carbon dioxide partial pressure were significantly lower than that of the comparison group, while the oxygen partial pressure and oxygen saturation was significantly higher than the comparison group,observation group mortality rate was 2.74%, significantly lower than the comparison group which was 7.41%.
Aged ; Ambulances ; Cerebral Infarction ; complications ; therapy ; Female ; Humans ; Male ; Middle Aged ; Sleep Apnea, Obstructive ; complications ; therapy ; Transportation of Patients ; Ventilators, Mechanical

Humans ; Maintenance Chemotherapy ; Multiple Myeloma ; therapy

Hydroxyapatite artificial eye mount has no toxicity,no sensitization and good histocompatibility.Its porous structure is beneficial for the ingrowth of new vessels and fibrous tissues,thereby it is the main implant of orbital reconstruction following enucleation.With the wide application in clinics,hydroxyapatite artificial eye mount also show some complications such as conjunctival humbling,erosion,split and artificial eye mount exposure.There are many causes for these complications and the most one is the stimulation and wearing of orbital soft tissues induced by crude implants,as well as the delaying in vessels.Using autologous or variant tissue for covering or encapsulating the anterior artificial eye mount,the mount will be strengthen and become smooth,thereby reducing the friction.Autologous sclera is superior to other encapsulating materials.

In the current multimedia application of medical theory teaching there exist many problems such as infusion,relation estrangedness between teachers and students,the moral education marginalization,the abstract thinking ability attenuation and so on.Facing these problems,in the medical theory teaching,the multimedia application should take"help","reality","transfer"as the value pursue,and be located in the exaggeration atmosphere,the interest stimulation,induction inspiration,difficulty breakthrough,thought expansion and memory optimization,emotion cultivation and moral promotion.

ObjectiveTo study the clinical significance of serum type Ⅳ collagen( Ⅳ - C) levelsfor the diagnosis of hepatic fibrosis. MethodsSerum levels of Ⅳ - C were measured in 209 patientswith viral hepatitis and 53 healthy persons by ELISA. ResultsCompared with the normal group,the serum Ⅳ - C level was significantly increased in patients with chronic moderate hepatitis and wasthe highest in patients with chronic severe hepatitis and hepatic cirrhosis( P < 0.01 ). There was no sig-nificant difference in the serum Ⅳ- C level among acute viral hepatitis, chronic mild hepatitis and con-trols ( P >0.05). There was significant difference in the serum Ⅳ - C level in patients with hepaticcirrhosis by Child- Pugh grading( P < 0.01 ). The serum Ⅳ - C level was positively correlated withthe degree of hepatic fibrosis and the severity of hepatic diseases. ConclusionThe meastrement of serumⅣ- C may be useful for assessment of the degree of hepatic fibrosis and progrosis of chronic hepatitis.

Objective To investigate the characteristics of lung involvement in microscopic polyangiitis(MPA)patients and its influence on treatment and prognosis.Methods From Jan 2006 to Dec 2007,29 patients were diagnosed with MPA in Peking Union Medical College Hospital.Their clinical data,including clinical manifestations,radiologic and laboratory test results,were retrospectively analyzed.Results The patients included 14 males and 15 females.The mean age was(63.7?10.1)years;28 patients(96.6%)were involved in the lung;20 patients(69.0%)had respiratory symptoms and 15 patients(51.7%)had respiratory signs.Two kinds of patterns were found in radiology:hemorrhage and fibrosis.Seventeen patients(17/27)were hypoxemia in room air blood gas analysis.Five patients(5/18)were diagnosed with pulmonary hypertension by echocardiograghy.Seven patients(7/10)were abnormal in lung function test.Four patients(4/6)were abnormal in BALF.Ten patients(34.5%)were diagnosed with alveolar hemorrhage and 18 patients(62.1%)were complicated with pulmonary infection.Totally 10 patients(34.5%)died,2 patients of diffuse alveolar hemorrhage and 8 of pulmonary infection.Conclusion Most MPA patients have pulmonary involvement and the clinical manifestations vary.There is a high mortality in MPA patients and the main causes are pulmonary infection and diffuse alveolar hemorrhage.